Near-infrared fluorescent dyes with large stokes shifts
09958451 ยท 2018-05-01
Assignee
Inventors
- Robert Michael Strongin (Portland, OR, US)
- Martha Sibrian-Vazquez (Portland, OR)
- Jorge Omar Escobedo-Cordova (Portland, OR, US)
- Mark Allen Lowry (Portland, OR, US)
Cpc classification
Y10T436/141111
GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
C09B11/22
CHEMISTRY; METALLURGY
C09B11/12
CHEMISTRY; METALLURGY
C09B11/24
CHEMISTRY; METALLURGY
G01N21/6486
PHYSICS
International classification
C09B11/12
CHEMISTRY; METALLURGY
C09B11/24
CHEMISTRY; METALLURGY
C09B11/22
CHEMISTRY; METALLURGY
Abstract
Embodiments of near-infrared (NIR) dyes are disclosed, along with methods and kits for detecting analytes with the NIR dyes. The NIR dyes have a structure according to the general structure ##STR00001##
At least one of R.sup.1/R.sup.2, R.sup.2/R.sup.3, R.sup.3/R.sup.4, R.sup.5/R.sup.6, R.sup.6/R.sup.7, and/or R.sup.7/R.sup.8 together forms a substituted or unsubstituted cycloalkyl or aryl.
Claims
1. A compound having a chemical structure selected from ##STR00034## ##STR00035##
2. A kit for detecting an analyte, comprising at least one compound according to claim 1, wherein the compound when combined with a sample comprising the analyte will undergo a change in its absorbance spectrum, emission spectrum, or both compared to the compound in a sample that does not comprise the analyte.
3. The kit of claim 2, further comprising at least one buffer solution in which the compound when combined with a sample comprising the analyte will undergo a change in its absorbance spectrum, emission spectrum, or both compared to the compound combined with the buffer solution and a sample that does not comprise the analyte.
4. The kit of claim 2, where the analyte comprises glutathione, cysteine, homocysteine, succinyl-5-amino-4-imidazolecarboxamide riboside, succinyladenosine, or a combination of succinyl-5-amino-4-imidazolecarboxamide riboside and succinyladenosine.
5. The kit of claim 2, further comprising a plurality of disposable containers in which a reaction between the compound and the analyte can be performed.
6. The kit of claim 5, where an amount of the compound effective to undergo a detectable change in the absorbance spectrum, the emission spectrum, or both when reacted with the analyte is premeasured into the plurality of disposable containers.
7. A method for selectively detecting an analyte in a biological fluid, comprising: (a) combining a compound according to general formula (III) or (IV) with a biological fluid to form a solution ##STR00036## where each bond depicted as is a single or double bond as needed to satisfy valence requirements; X.sup.1 is O, S, or N(H); R.sup.6 is hydroxyl, amino, alkyl amino, or NHR.sup.c when the bond between R.sup.6 and ring A is a single bond, or R.sup.6 is oxygen, imino, iminium, alkyl imino, or alkyl iminium when the bond between R.sup.6 and ring A is a double bond, where R.sup.C is ##STR00037## R.sup.7 is hydrogen or halogen; R.sup.9-R.sup.12 independently are hydrogen, lower alkyl, carboxyl, amino, or SO.sub.3H; R.sup.13 is hydrogen, lower alkyl, lower alkoxy, SO.sub.3H or COOR.sup.14 where R.sup.14 is hydrogen or lower alkyl and the bond depicted as
in ring B is a double bond, or R.sup.13 is one or more atoms forming a ring system with rings B and D and the bond depicted as
in ring B is a single bond; R.sup.16 and R.sup.18 independently are hydrogen, hydroxyl, thiol, oxygen, lower alkoxy, amino, alkyl amino, or NHR.sup.c, and at least one of R.sup.16 and R.sup.18 is other than hydrogen; R.sup.19 and R.sup.21 independently are hydrogen, hydroxyl, thiol, oxygen, amino, alkyl amino, imino, iminium, alkyl imino, alkyl iminium, or NHR.sup.c where R.sup.c is as defined above, and at least one of R.sup.19 and R.sup.21 is other than hydrogen; and wherein if X.sup.1 is oxygen in general formula (III), then R.sup.6 is other than oxygen or alkyl amino, or R.sup.13 is other than one or more atoms forming a ring system with rings B and D, or R.sup.16 is other than hydroxyl or hydrogen, or R.sup.18 is other than hydroxyl or hydrogen, or at least one of R.sup.7, R.sup.9, R.sup.10, R.sup.11, R.sup.12, R.sup.13 is other than hydrogen, or if the compound has a chemical structure according to general formula (IV), then at least one of R.sup.9-R.sup.13, R.sup.16, R.sup.18, R.sup.19, or R.sup.21is other than hydrogen, hydroxyl, halogen, oxygen, lower alkyl, amino, or thiol, or R.sup.13 is one or more atoms forming a ring system with rings B and D and the bond depicted as
in ring B is a single bond; (b) exposing the solution to a light source; and (c) detecting the analyte by detecting fluorescence from the compound, wherein the analyte comprises cysteine, homocysteine, glutathione, succinyl-5-amino-4-imidazolecarboxamide riboside, succinyladenosine, or a combination thereof.
8. The method of claim 7 where detecting fluorescence from the compound comprises detecting fluorescence at a wavelength corresponding to an emission spectrum maximum of the compound.
9. The method of claim 8, further comprising quantitating the analyte by measuring an amount of fluorescence from the compound at a wavelength corresponding to an emission spectrum maximum of the compound.
10. The method of claim 7 where the biological fluid comprises blood or urine.
Description
BRIEF DESCRIPTION OF THE DRAWINGS
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DETAILED DESCRIPTION
(33) Disclosed herein are embodiments of novel near-infrared (NIR) dyes based on xanthene architectures. Xanthene dyes such as fluorescein and rhodamine derivatives are commonly used fluorescent dyes due to their bright fluorescence and compatibility with common laser line excitations. Fluorescein emission generally falls in the green or yellow spectral region while some rhodamines exhibit red emission (>600 nm).
(34) Embodiments of the disclosed NIR dyes form a unique series of xanthene-based regioisomeric naphthofluorone dyes exhibiting a combination of desirable characteristics, including (i) relatively low molecular weight, (ii) aqueous solubility, and/or (iii) dual excitation and emission from their fluorescent neutral and anionic forms. Systematic changes in the regiochemistry of benzannulation and the ionizable moieties afford (iv) tunable deep-red to NIR emission and (v) enhanced Stokes shifts.
(35) I. Terms and Definitions
(36) The following explanations of terms and abbreviations are provided to better describe the present disclosure and to guide those of ordinary skill in the art in the practice of the present disclosure. As used herein, comprising means including and the singular forms a or an or the include plural references unless the context clearly dictates otherwise. The term or refers to a single element of stated alternative elements or a combination of two or more elements, unless the context clearly indicates otherwise.
(37) Unless explained otherwise, all technical and scientific terms used herein have the same meaning as commonly understood to one of ordinary skill in the art to which this disclosure belongs. Although methods and materials similar or equivalent to those described herein can be used in the practice or testing of the present disclosure, suitable methods and materials are described below. The materials, methods, and examples are illustrative only and not intended to be limiting. Other features of the disclosure are apparent from the following detailed description and the claims.
(38) Unless otherwise indicated, all numbers expressing quantities of ingredients, properties such as molecular weight, percentages, and so forth, as used in the specification or claims are to be understood as being modified by the term about. Unless otherwise indicated, non-numerical properties such as amorphous, crystalline, homogeneous, and so forth as used in the specification or claims are to be understood as being modified by the term substantially, meaning to a great extent or degree. Accordingly, unless otherwise indicated, implicitly or explicitly, the numerical parameters and/or non-numerical properties set forth are approximations that may depend on the desired properties sought, limits of detection under standard test conditions/methods, limitations of the processing method, and/or the nature of the parameter or property. When directly and explicitly distinguishing embodiments from discussed prior art, the embodiment numbers are not approximates unless the word about is recited.
(39) Definitions of common terms in chemistry may be found in Richard J. Lewis, Sr. (ed.), Hawley's Condensed Chemical Dictionary, published by John Wiley & Sons, Inc., 1997 (ISBN 0-471-29205-2). Definitions of common terms in molecular biology may be found in Benjamin Lewin, Genes VII, published by Oxford University Press, 2000 (ISBN 019879276X); Kendrew et al. (eds.), The Encyclopedia of Molecular Biology, published by Blackwell Publishers, 1994 (ISBN 0632021829); and Robert A. Meyers (ed.), Molecular Biology and Biotechnology: a Comprehensive Desk Reference, published by Wiley, John & Sons, Inc., 1995 (ISBN 0471186341); and other similar references.
(40) In order to facilitate review of the various embodiments of the disclosure, the following explanations of specific terms are provided:
(41) Absorbance is the retention by a compound or substance of certain wavelengths of radiation incident upon it; a measure of the amount of light at a particular wavelength absorbed as the light passes through a compound or substance, or through a solution of a compound or substance.
(42) Alkyl refers to a hydrocarbon group having a saturated carbon chain. The chain may be cyclic, branched or unbranched. The term lower alkyl means the chain includes 1-10 carbon atoms.
(43) An analogue or derivative is a molecule that differs in chemical structure from a parent compound, for example a homologue (differing by an increment in the chemical structure, such as a difference in the length of an alkyl chain), a molecular fragment, a structure that differs by one or more functional groups, a change in ionization. Structural analogues are often found using quantitative structure activity relationships (QSAR), with techniques such as those disclosed in Remington (The Science and Practice of Pharmacology, 19th Edition (1995), chapter 28).
(44) Annelation/annulation is a chemical reaction in which one cyclic or ring structure is added to another to form a polycyclic, or annulated, compound. Annulation can be categorized as type [a], type [b], or type [c], depending on the position of the newly added ring. Type [a] refers to down annulation, type [b] refers to across annulation, and type [c] refers to up annulation, as shown below:
(45) ##STR00014##
(46) As used herein, the term annulated refers to having or consisting of rings or ringlike segments. The term benzannulated refers to derivatives of cyclic compounds (usually aromatic), which are fused to a benzene ring. Examples of benzannulated compounds include, inter alia, benzopyrenes, quinolines, naphthoquinones, naphthofluoresceins, rhodamines, and xanthenes.
(47) Aromatic or aryl compounds typically are unsaturated, cyclic hydrocarbons having alternate single and double bonds. Benzene, a 6-carbon ring containing three double bonds, is a typical aromatic compound.
(48) A bathochromic shift is a change of spectral band position in the absorption, reflectance, transmittance, or emission spectrum of a molecule to a longer wavelength, or lower frequency. A bathochromic shift commonly is referred to as a red shift. Bathochromic shifts can occur in the spectra of a series of structurally related molecules with different substitutions and/or substitution patterns. A change in environment, e.g., solvent polarity, also can produce a bathochromic shift.
(49) Coumarin: A benzopyrone having the following general structure:
(50) ##STR00015##
(51) As used herein, substituted coumarin refers to a coumarin including one or more substituents. Suitable substituents may include substituted or unsubstituted lower alkyl, substituted or unsubstituted lower alkoxy, lower alkyl carbonyl, substituted or unsubstituted amino, halo, and hydroxyl groups. In some embodiments, the coumarin is substituted at the 3- and/or 7-position with, for example, a lower alkyl, lower alkyl carbonyl, lower alkoxy, amino or substituted amino group.
(52) Emission or an emission signal refers to the light of a particular wavelength generated from a source. In particular examples, an emission signal is emitted from a fluorophore after the fluorophore absorbs light at its excitation wavelength(s).
(53) Excitation or an excitation signal refers to the light of a particular wavelength necessary and/or sufficient to excite an electron transition to a higher energy level. In particular examples, an excitation signal is the light of a particular wavelength necessary and/or sufficient to excite a fluorophore to a state such that the fluorophore will emit a different (such as a longer) wavelength of light than the wavelength of light from the excitation signal.
(54) Fluorescence is the emission of visible radiation by an atom or molecule passing from a higher to a lower electronic state, wherein the time interval between absorption and emission of energy is 10.sup.8 to 10.sup.3 second. As used herein, fluorescence occurs when the atom or molecule absorbs energy from an excitation source (e.g., a lamp producing light within a wavelength range of 190-850 nm) and then emits the energy as visible and/or near-infrared radiation.
(55) A fluorogen is a compound capable of fluorescence.
(56) A fluorophore is the functional group, or portion, of a molecule that causes the molecule to fluoresce when exposed to an excitation source. The term fluorophore also is used to refer to fluorescent compounds used as dyes to mark proteins with a fluorescent label.
(57) A functional group is a specific group of atoms within a molecule that is responsible for the characteristic chemical reactions of the molecule. Exemplary functional groups include, without limitation, alkane, alkene, alkyne, arene, halo (fluoro, chloro, bromo, iodo), epoxide, hydroxyl, carbonyl (ketone), aldehyde, carbonate ester, carboxylate, ether, ester, peroxy, hydroperoxy, carboxamide, amine (primary, secondary, tertiary), ammonium, imide, azide, cyanate, isocyanate, thiocyanate, nitrate, nitrite, nitrile, nitroalkane, nitroso, pyridyl, phosphate, sulfonyl, sulfide, thiol (sulfhydryl), disulfide.
(58) GSH: Glutathione.
(59) Heteroaryl compounds are aromatic compounds having at least one heteroatom, i.e., one or more carbon atoms in the ring has been replaced with an atom having at least one lone pair of electrons, typically nitrogen, oxygen, or sulfur.
(60) Imino refers to a functional group having the formula NH.
(61) Iminium refers to a protonated or substituted imine, e.g., NH.sub.2.sup.+ or (NR.sub.AR.sub.B).sup.+ where R.sub.A and R.sub.B represent alkyl or substituted alkyl groups. As used herein, (NR.sub.AR.sub.B).sup.+ is referred to as an alkyl iminium group.
(62) MIP: Molecule-imprinted polymer.
(63) Near infrared (NIR) is a region of the electromagnetic spectrum between the visible region and the infrared region. There is no set definition for the boundaries of the near-infrared region, but definitions include the wavelength ranges from 650-2500 nm, 750-2500 nm, 780-2500 nm, 800-2500 nm, 700-1400 nm, or 780-3000 nm. As used herein, NIR typically refers to the wavelength region of 700-1400 nm.
(64) A rhodol is a structural hybrid of fluorescein and a rhodamine. Rhodamines are a family of related fluorone dyes. The structures of fluorescein, a rhodamine, and two rhodol analogues are shown below.
(65) ##STR00016##
(66) SNAFL: Seminaphthofluorescein
(67) SNAFR: Seminaphthofluorone
(68) SNARF: Seminaphthorhodafluor
(69) Stokes shift refers to the difference (in wavelength or frequency units) between absorbance spectrum maximum and the emission spectrum maximum of the same electronic transition. Typically, the wavelength of maximum fluorescence emission is longer than that of the exciting radiation, i.e., the wavelength of maximum absorbance.
(70) Xanthene is an organic heterocyclic compound with the formula C.sub.13H.sub.10O.
(71) ##STR00017##
Xanthene derivatives are referred to as xanthenes, and include fluorescein, rhodamine, and derivatives thereof.
II. Overview Of Representative Embodiments
(72) Embodiments of compounds having emission spectrum maxima in the near-infrared region (near-infrared (NIR) dyes) and that selectively detect analytes in buffered solutions and/or biological media are disclosed. Some embodiments of the disclosed compounds exhibit large bathochromic shifts and/or enhanced Stokes shifts compared to currently available NIR dyes.
(73) Some embodiments of the disclosed compounds have a structure according to general formulas (III)-(XI) as described herein. In some embodiments, X.sup.1 is oxygen. In any or all of the above embodiments, R.sup.13 may be COO and form a lactone ring. In any or all of the above embodiments, the compound may comprise at least one halogen atom positioned adjacent to an ionizable moiety.
(74) In any or all of the above embodiments, the compound may have an emission spectrum maximum at a wavelength greater than or equal to 700 nm, such as greater than or equal to 750 nm. In any or all of the above embodiments, the compound may have a Stokes shift greater than or equal to 80 nm, such as greater than or equal to 100 nm.
(75) In some embodiments, the compound has a chemical structure according to structure (III) where R.sup.6 is amino or alkyl amino, and at least one of R.sup.16 or R.sup.18 is hydroxyl, amino, or alkyl amino. In other embodiments, the compound has a chemical structure according to structure (III), where R.sup.1, R.sup.2, R.sup.5, R.sup.7, and R.sup.8 are hydrogen or halogen; R.sup.6 is oxygen, imino, iminium, lower alkyl iminium, or NHR.sup.c, and R.sup.9-R.sup.12 independently are hydrogen, amino, lower alkyl, carboxyl, or SO.sub.3H. In still other embodiments, the compound has a chemical structure according to structure (III) where R.sup.1, R.sup.2, R.sup.5, R.sup.7-R.sup.12, and R.sup.15-R.sup.17 are hydrogen, R.sup.6 is iminium, R.sup.13 is COOR.sup.14 where R.sup.14 is hydrogen or lower alkyl, and R.sup.18 is amino.
(76) In some embodiments, the compound has a chemical structure according to structure (IV), (IX), or (X) where X.sup.1 is oxygen and R.sup.18 is halogen, hydroxyl, thiol, amino, alkyl amino, or alkoxy. In some embodiments, the compound has a chemical structure according to structure (IV) where X.sup.1 is oxygen and R.sup.19 is hydroxyl, thiol, oxygen, imino, iminium, alkyl imino, alkyl iminium, amino, or alkyl amino; in certain embodiments, R.sup.18 is halogen, hydroxyl, thiol, amino, alkyl amino, or alkoxy.
(77) In some embodiments, the compound has a chemical structure according to structure (VI) where R.sup.17 is halogen, hydroxyl, thiol, amino, alkyl amino, or NHR.sup.c, and R.sup.20 is oxygen, sulfur, imino, iminium, alkyl iminium, or NHR.sup.c.
(78) Embodiments of a method for selectively detecting an analyte in a biological fluid include combining a compound according to any or all of the above embodiments with a biological fluid to form a solution, exposing the solution to a light source, and detecting the analyte by detecting fluorescence from the compound. In some embodiments, the light source has a wavelength in the range of 190 nm to 850 nm. In any or all of the above embodiments, the biological fluid may comprise blood or urine.
(79) In any or all of the above embodiments, detecting fluorescence from the compound may include detecting fluorescence at a wavelength corresponding to an emission spectrum maximum of the compound. In some embodiments, the compound has an emission spectrum maximum at a wavelength greater than or equal to 700 nm, such as greater than or equal to 750 nm. In some embodiments, the analyte is quantitated by measuring an amount of fluorescence from the compound at a wavelength corresponding to an emission spectrum maximum of the compound.
(80) In any or all of the above embodiments, the analyte may be cysteine, homocysteine, glutathione, succinyl-5-amino-4-imidazolecarboxamide riboside, succinyladenosine, or a combination thereof. In some embodiments, the compound has a chemical structure according to general structure (VI) where R.sup.1, R.sup.4, R.sup.5 and R.sup.8 independently are hydrogen, hydroxyl, thiol, lower alkyl, carboxyalkyl, amino, substituted amino, alkoxy, or halogen; and R.sup.19-R.sup.22 independently are hydrogen, hydroxyl, thiol, halogen, oxygen, imino, iminium, alkyl imino, alkyl iminium, amino, alkyl amino, or NHR.sup.c.
(81) In some embodiments, the analyte is glutathione, and the compound has a structure according to general structure (VI) as described above where R.sup.17 is hydroxyl, amino, or alkyl amino, and R.sup.20 is oxygen, hydroxyl, amino, alkyl amino, imino, or alkyl iminium. In one embodiment, the compound is
(82) ##STR00018##
(83) In some embodiments, the analyte is succinyl-5-amino-4-imidazole-carboxamide riboside, succinyladenosine, or a combination thereof, and the compound has a structure according to general structure (VI) as described above where at least one of R.sup.17 and R.sup.20 is NHR.sup.c, and R.sup.13 is COOR.sup.14 where R.sup.14 is hydrogen or lower alkyl. In some embodiments, the analyte is succinyl-5-amino-4-imidazolecarboxamide riboside, succinyladenosine, or a combination thereof, and the compound comprises at least one fluorophore according to general formula (III) where X.sup.1 is oxygen and R.sup.6 and R.sup.16 are NHR.sup.c or general formula (IV) where X.sup.1 is oxygen and R.sup.16 and R.sup.21 are NHR.sup.c.
(84) Embodiments of a kit for detecting an analyte include at least one compound according to any or all of the above embodiments, wherein the compound when combined with a sample comprising the analyte will undergo a change in its absorbance spectrum and/or emission spectrum compared to the compound in a sample that does not comprise the analyte. In some embodiments, the sample is a biological fluid. In any or all of the above embodiments, the kit may include at least one buffer solution in which the compound when combined with a sample comprising the analyte will undergo a change in its absorbance spectrum and/or emission spectrum compared to the compound combined with the buffer solution and a sample that does not comprise the analyte. In any or all of the above embodiments, the analyte may be glutathione, cysteine, homocysteine, succinyl-5-amino-4-imidazolecarboxamide riboside, succinyladenosine, or a combination of succinyl-5-amino-4-imidazolecarboxamide riboside and succinyladenosine. In some embodiments, the compound in the kit has a chemical structure according to general structure (VI) as described above. In some embodiments, the compound in the kit has a chemical structure according to general structure (III) as described above where X.sup.1 is oxygen and R.sup.6 and R.sup.16 are NHR.sup.c or general structure (IV) where X.sup.1 is oxygen and R.sup.16 and R.sup.21 are NHR.sup.c.
(85) In any or all of the above embodiments, the kit may include a color comparison chart for evaluating a color change produced by a reaction between the compound and the analyte. In any or all of the above embodiments, the kit may include a plurality of disposable containers in which a reaction between the compound and the analyte can be performed. In some embodiments, an amount of the compound effective to undergo a detectable change in the absorbance spectrum, the emission spectrum, or both when reacted with the analyte is premeasured into the plurality of disposable containers.
(86) III. Near-Infrared Dyes
(87) Embodiments of the disclosed near-infrared (NIR) dyes are based on an annulated xanthene architecture. Some embodiments of the disclosed NIR dyes exhibit significant bathochromic shifts and enhanced Stokes shifts compared to structurally related analogues. In some embodiments, the NIR dyes are benzannulated xanthenes with single or double annulation. The annulation may be type [a]down, type [b]across, or type [c]up. At least some embodiments of the singly and doubly annulated NIR dyes have substantially red-shifted absorbance and emission spectra compared to commercially available NIR dyes. In some embodiments, the spectra are red shifted by at least 100 nm. In particular, some embodiments of the disclosed NIR dyes have an emission maximum greater than 700 nm. In certain embodiments, emission maxima are well into the near-infrared region, e.g., greater than 700 nm, greater than 750 nm, and even greater than 780 nm. In comparison, some known annulated xanthenes have emission maxima near 560 nm (fully benzannulated dibenzofluorescein), 630 nm (SNAFLs), 650 nm (SNARFs), 670 nm (naphthofluorescein), or 650-675 nm (dibenzorhodamines). In some embodiments, the absorption maxima are greater than 600 nm, greater than 650 nm, greater than 675 nm, and even greater than 800 nm.
(88) Some embodiments of the NIR dyes also exhibit an enhanced Stokes shift compared to known long-wavelength xanthenes. In certain embodiments, the Stokes shift (difference between absorbance spectrum maximum and the emission spectrum maximum of the same electronic transition) is greater than 50 nm, greater than 80 nm, greater than 100 nm, or greater than 150 nm, such as 50-200 nm, 50-150 nm, 80-150 nm, 90-170 nm, 100-150 nm, or 100-200 nm. A large Stokes shift is advantageous since it facilitates use of relatively broad-band light sources, such as light-emitting diodes, with minimal scattered light interference from the light source at the emission wavelength(s) measured.
(89) A. Structures
(90) In some embodiments, the NIR dyes have a structure according to general formula I.
(91) ##STR00019##
In general formula I, each bond depicted as is a single or double bond as needed to satisfy valence requirements; X.sup.1 is O, S, Se, Si(CH.sub.3).sub.2, Ge(CH.sub.3).sub.2, Sn(CH .sub.3).sub.2, CH.sub.2, C(CH.sub.3).sub.2 or NH. R.sup.1, R.sup.2, and R.sup.4 independently are hydrogen, hydroxyl, oxygen, thiol, lower alkyl, carboxyalkyl, amino, substituted amino, alkoxy, halogen, or NHR.sup.c where R.sup.c is
(92) ##STR00020##
R.sup.5, R.sup.7, and R.sup.8 independently are hydrogen, hydroxyl, thiol, lower alkyl, carboxyalkyl, amino, substituted amino, alkoxy, or halogen; R.sup.3 and R.sup.6 independently are hydrogen, hydroxyl, halogen, oxygen, sulfur, thiol, amino, alkyl amino, imino, iminium, alkyl imino, alkyl iminium, cycloalkyl imino, or NHR.sup.c where R.sup.c is as defined above; and at least one of R.sup.1 and R.sup.2, R.sup.2 and R.sup.3, R.sup.3 and R.sup.4, R.sup.5 and R.sup.6, R.sup.6 and R.sup.7, and/or R.sup.7 and R.sup.8 together form a substituted or unsubstituted cycloalkyl or aryl. R.sup.9-R.sup.12 independently are hydrogen, alkyl, acyl, carboxyl, nitro, amino, alkyl amino, or SO.sub.3H. R.sup.13 is hydrogen, hydroxyl, lower alkyl, lower alkoxy, SO.sub.3H or COOR.sup.14 where R.sup.14 is hydrogen or lower alkyl and the bond depicted as in ring B is a double bond, or R.sup.13 is one or more atoms forming a ring system with rings B and D and the bond depicted as
in ring B is a single bond. In some embodiments, R.sup.13 is COO and forms a lactone ring as shown in general formula II.
(93) ##STR00021##
(94) In some embodiments, R.sup.3 and R.sup.4 and/or R.sup.5 and R.sup.6 together form an aryl or substituted aryl ring as shown in general formulas III and IV
(95) ##STR00022##
where each bond depicted as is a single or double bond as needed to satisfy valence requirements. R.sup.1-R.sup.14 and X.sup.1 are as defined for general formulas (I) and (II). R.sup.15R.sup.22 independently are hydrogen, halogen, hydroxyl, oxygen, thiol, amino, alkyl amino, alkoxy, such as lower alkoxy, sulfur, imino, iminium, alkyl imino, alkyl iminium, or NHR.sup.c where R.sup.c is as defined above. R.sup.19, R.sup.20, R.sup.21, and R.sup.22 independently are hydrogen, hydroxyl, thiol, halogen, oxygen, amino, alkyl amino, or NHR.sup.c where R.sup.c is as defined above. In certain embodiments, X.sup.1 is oxygen, R.sup.1, R.sup.2, R.sup.5, R.sup.7 and R.sup.8 independently are hydrogen or halogen; R.sup.6 is oxygen, imino, iminium, lower alkyl iminium, or NHR.sup.c where R.sup.c is as defined above; R.sup.9-R.sup.12 independently are hydrogen, amino, lower alkyl, carboxyl, or SO.sub.3H; R.sup.15, R.sup.17, R.sup.20, and R.sup.22 are hydrogen; R.sup.13 is hydrogen, lower alkyl, lower alkoxy, SO.sub.3H, COOR.sup.14 where R.sup.14 is hydrogen or lower alkyl and the bond depicted as
in ring B is a double bond, or R.sup.13 is one or more atoms forming a ring system with rings B and D and the bond depicted as
in ring B is a single bond; R.sup.16 and R.sup.18 independently are hydrogen, hydroxyl, oxygen, lower alkoxy, amino, alkyl amino, or NHR.sup.c where R.sup.c is as defined above, and at least one of R.sup.16 and R.sup.18 is other than hydrogen; R.sup.19 and R.sup.21 independently are oxygen, imino, iminium, lower alkyl iminium, or NHR.sup.c where R.sup.c is as defined above, and at least one of R.sup.19 and R.sup.21 is other than hydrogen. In particular embodiments, X.sup.1 is oxygen, R.sup.1, R.sup.2, R.sup.5, R.sup.8, R.sup.9, R.sup.12, R.sup.15, R.sup.17, R.sup.20, and R.sup.22 are hydrogen; R.sup.6 is oxygen, imino, iminium, lower alkyl iminium, or NHR.sup.c where R.sup.c is as defined above; R.sup.7 is hydrogen or halogen; R.sup.10 and R.sup.11 independently are hydrogen, amino, lower alkyl, carboxyl, or SO.sub.3H; R.sup.13 is hydrogen, lower alkyl, lower alkoxy, SO.sub.3H, or COOR.sup.14 where R.sup.14 is hydrogen or lower alkyl and the bond depicted as
in ring B is a double bond, or R.sup.13 is one or more atoms forming a ring system with rings B and D and the bond depicted as
in ring B is a single bond; R.sup.16 and R.sup.18 independently are hydrogen, hydroxyl, oxygen, lower alkoxy, amino, alkyl amino, or NHR.sup.c where R.sup.c is as defined above, and at least one of R.sup.16 and R.sup.18 is other than hydrogen; R.sup.19 is hydrogen, hydroxyl, oxygen, imino, iminium, or lower alkyl iminium; R.sup.21 is hydrogen, oxygen, or NHR.sup.c where R.sup.c is as defined above, and at least one of R.sup.19 and R.sup.21 is other than hydrogen. In certain embodiments, if X.sup.1 is oxygen in structure III, then R.sup.6 is other than oxygen or alkyl amino, or R.sup.13 is other than one or more atoms forming a ring system with rings B and D, or R.sup.16 is other than hydroxyl or hydrogen, or R.sup.18 is other than hydroxyl or hydrogen, or at least one of R.sup.1, R.sup.2, R.sup.5, R.sup.7, R.sup.8, R.sup.9, R.sup.10, R.sup.11, R.sup.12, or R.sup.13 is other than hydrogen. In certain embodiments, if X.sup.1 is oxygen, sulfur, CH.sub.2, or NH in structure IV, then at least one of R.sup.1, R.sup.2, R.sup.7-R.sup.13, or R.sup.15-R.sup.22 is other than hydrogen, hydroxyl, halogen, lower alkyl, amino, or thiol, or R.sup.13 is one or more atoms forming a ring system with rings B and D and the bond depicted as
in ring B is a single bond. Representative compounds according to general formulas III and IV are shown in Table 1.
(96) TABLE-US-00001 TABLE 1 Cpd R.sup.6* R.sup.7 R.sup.10 R.sup.11 R.sup.13 R.sup.16 R.sup.18 R.sup.19 R.sup.21 3a O H H H OCH.sub.3 H OH 3b O H CH.sub.3 H CH.sub.3 H OH 3c O H H COOH H H OH 3d O H H NH.sub.2 H H OH 3e O H H H SO.sub.3H H OH 3f O H SO.sub.3H H SO.sub.3H H OH 3g O F H H H H OH 3h NH H SO.sub.3H H SO.sub.3H H NH.sub.2 3i N(CH.sub.3).sub.2.sup.+ H H H H H OH 3j N(CH.sub.3).sub.2.sup.+ H H H H H N(CH.sub.3).sub.2 3k N(CH.sub.3).sub.2.sup.+ H SO.sub.3H H SO.sub.3H H N(CH.sub.3).sub.2 4a H CH.sub.3 H CH.sub.3 H OH O H 4b H SO.sub.3H H SO.sub.3H H OH O H 4c H CH.sub.3 H CH.sub.3 H NH.sub.2 NH H 4d H CH.sub.3 H CH.sub.3 H N(CH.sub.3).sub.2 N(CH.sub.3).sub.2.sup.+ H 4e H H COOH H H OH O H 4f H H COOH H H N(CH.sub.3).sub.2 N(CH.sub.3).sub.2.sup.+ H 7 H H H COO.sup. H OH OH H 7a H H H COO.sup. H OH OH H 7b H H H COO.sup. H O OH H 8 H H H COOCH.sub.3 H OH O H 8a H H H COOCH.sub.3 H O.sup. O H 9 H H H COOCH.sub.3 H OCH.sub.3 O H 10 H CH.sub.3 H CH.sub.3 H OH O H 11 H H SO.sub.3H SO.sub.3H H OH O H 14a OH H H H COO.sup. H OH 14b NH.sub.2 H H H COO.sup. H OH 14c NH.sub.2 H H H COO.sup. H OCH.sub.3 14d N(CH.sub.3).sub.2 H H H COO.sup. H OH 14e OCH.sub.3 H H H COO.sup. H OH 14f OH H H H COO.sup. H NH.sub.2 14g OH H H H COO.sup. H N(CH.sub.3).sub.2 14h NH.sub.2 H H H COO.sup. H NH.sub.2 15a O H H H COOCH.sub.3 H OH 15b NH.sub.2.sup.+ H H H COOCH.sub.3 H OH 15c NH.sub.2.sup.+ H H H COOCH.sub.3 H OCH.sub.3 15d N(CH.sub.3).sub.2.sup.+ H H H COOCH.sub.3 H OH 15e O H H H COOCH.sub.3 H NH.sub.2 15f O H H H COOCH.sub.3 H N(CH.sub.3).sub.2 15g NH.sub.2.sup.+ H H H COOCH.sub.3 H NH.sub.2 15h O H H H COOCH.sub.3 H O.sup. 16a O H H H COOCH.sub.3 H OCH.sub.3 16b N(CH.sub.3).sub.2.sup.+ H H H COOCH.sub.3 H OCH.sub.3 19a OH H H H COO.sup. OH H 20a O H H H COOCH.sub.3 OH H 20b .sup.+NH.sub.2 H H H COOCH.sub.3 NH.sub.2 H 20c O H H H COOCH.sub.3 O.sup. H 20d O H H H COOH OH H 21a O H H H COOH OH H 21b O H H H COO O H 22 H H H COOCH.sub.3 OH H H O 22a H H H COOH OH H H O 40 H H H COOCH.sub.3 H OH O H 41 N(CH.sub.3).sub.2 H H H COO.sup. H N(CH.sub.3).sub.2 42 N(CH.sub.3).sub.2.sup.+ H H H COOCH.sub.3 H N(CH.sub.3).sub.2 50 NH.sub.2.sup.+ H H H COO.sup. NH.sub.2.sup.+ H 51 NHR.sup.c+ H H H COO.sup. NHR.sup.c+ H 52 H H H COO.sup. NH.sub.2.sup.+ H H NH.sub.2.sup.+ 53 H H H COO.sup. NHR.sup.c+ H H NHR.sup.c+ *All R substituents not included in Table 1 are hydrogen and X.sup.1 is oxygen. .sup.R.sup.11 forms a lactone ring.
(97) In some embodiments, R.sup.2 and R.sup.3 and/or R.sup.6 and R.sup.7 together form an aryl or substituted aryl ring as shown in general formulas V and VI
(98) ##STR00023##
where each bond depicted as is a single or double bond as needed to satisfy valence requirements, and R.sup.1-R.sup.22 and X.sup.1 are defined as above. In certain embodiments, X.sup.1 is oxygen, R.sup.1, R.sup.4, R.sup.5, R.sup.7 and R.sup.8 independently are hydrogen or halogen; R.sup.6 is oxygen, imino, iminium, lower alkyl iminium, or NHR.sup.c where R.sup.c is as defined above; R.sup.9-R.sup.12 independently are hydrogen, amino, lower alkyl, carboxyl, or SO.sub.3H; R.sup.15, R.sup.17, R.sup.19, and R.sup.22 are hydrogen; R.sup.13 is hydrogen, lower alkyl, lower alkoxy, SO.sub.3H, COOR.sup.14 where R.sup.14 is hydrogen or lower alkyl and the bond depicted as
in ring B is a double bond, or R.sup.13 is one or more atoms forming a ring system with rings B and D and the bond depicted as
in ring B is a single bond; at least one of R.sup.16, R.sup.17 and R.sup.18 is hydroxyl, oxygen, amino, alkyl amino, or NHR.sup.c where R.sup.c is as defined above; and at least one of R.sup.19, R.sup.20 and R.sup.21 is oxygen, imino, iminium, lower alkyl iminium, or NHR.sup.c where R.sup.c is as defined above. In particular embodiments, X.sup.1 is oxygen, R.sup.1, R.sup.4, R.sup.5, R.sup.8, R.sup.9, R.sup.12, R.sup.15, R.sup.16, R.sup.18, R.sup.19, R.sup.21, and R.sup.22 are hydrogen; R.sup.6 is oxygen, imino, iminium, lower alkyl iminium, or NHR.sup.c where R.sup.c is as defined above; R.sup.7 is hydrogen or halogen; R.sup.10 and R.sup.11 independently are hydrogen, amino, lower alkyl, carboxyl, or SO.sub.3H; R.sup.13 is hydrogen, lower alkyl, lower alkoxy, SO.sub.3H, or COOR.sup.14 where R.sup.14 is hydrogen or lower alkyl; R.sup.17 is hydroxyl, oxygen, amino, alkyl amino, or NHR.sup.c where R.sup.c is as defined above; and R.sup.20 is oxygen, imino, iminium, lower alkyl iminium, or NHR.sup.c where R.sup.c is as defined above. In certain embodiments, if X.sup.1 is oxygen and R.sup.15 or R.sup.17 is hydroxyl in structure V, then R.sup.6 is other than oxygen, amino, or alkyl amino, or at least one of R.sup.1, R.sup.8, or R.sup.18 is other than hydrogen, or R.sup.13 is one or more atoms forming a ring system with rings B and D and the bond depicted as
in ring B is a single bond. Representative compounds according to general formulas V and VI are shown in Table 2.
(99) TABLE-US-00002 TABLE 2 Cpd R.sup.6* R.sup.7 R.sup.10 R.sup.11 R.sup.13 R.sup.17 R.sup.20 2a O H H H OCH.sub.3 OH 2b O H CH.sub.3 H CH.sub.3 OH 2c O H H COOH H OH 2d O H H NH.sub.2 H OH 2e O H H H SO.sub.3H OH 2f O H SO.sub.3H H SO.sub.3H OH 2g O F H H H OH 2h NH H SO.sub.3H H SO.sub.3H NH.sub.2 2i N(CH.sub.3).sub.2.sup.+ H H H H OH 2j N(CH.sub.3).sub.2.sup.+ H H H H N(CH.sub.3).sub.2 2k N(CH.sub.3).sub.2.sup.+ H SO.sub.3H H SO.sub.3H N(CH.sub.3).sub.2 5a H CH.sub.3 H CH.sub.3 OH O 5b H SO.sub.3H H SO.sub.3H OH O 5c H CH.sub.3 H CH.sub.3 NH.sub.2 NH 5d H CH.sub.3 H CH.sub.3 N(CH.sub.3).sub.2 N(CH.sub.3).sub.2.sup.+ 5e H H COOH H OH O 5f H H COOH H N(CH.sub.3).sub.2 N(CH.sub.3).sub.2.sup.+ 24 H H H CH(OH)O OH NH.sub.2 34a H H COOH CH.sub.3 OH O 34b H H H COOH OH O 34c H H COOH CH.sub.3 OH N(CH.sub.3).sub.2.sup.+ 34d H H H COOH OH N(CH.sub.3).sub.2.sup.+ 43 H H H COO OH NH 44 H H H COO NH.sub.2 NH 45 H H H COOH OH NH 46 H H H COOH NH.sub.2 NH *All R substituents not included in Table 2 are hydrogen and X.sup.1 is oxygen. .sup.R.sup.11 forms a ring.
(100) In some embodiments, R.sup.1 and R.sup.2 and/or R.sup.7 and R.sup.8 together form an aryl or substituted aryl ring as shown in general formulas VII and VIII
(101) ##STR00024##
where each bond depicted as is a single or double bond as needed to satisfy valence requirements. R.sup.3-R.sup.18 and X.sup.1 in general formula VII are defined as above. R.sup.3-R.sup.5, R.sup.9-R.sup.22, and X.sup.1 in general formula VIII are defined as above, and R.sup.6 is hydrogen, hydroxyl, oxygen, halogen, thiol, amino, alkyl amino, or NHR.sup.c where R.sup.c is as defined above. In certain embodiments, X.sup.1 is oxygen, R.sup.3, R.sup.4, R.sup.5 independently are hydrogen or halogen; R.sup.7 and R.sup.8, if present, independently are hydrogen or halogen; R.sup.9-R.sup.12 independently are hydrogen, amino, lower alkyl, carboxyl, or SO.sub.3H; R.sup.15, R.sup.17, R.sup.19, and R.sup.22 are hydrogen; R.sup.13 is hydrogen, lower alkyl, lower alkoxy, SO.sub.3H, COOR.sup.14 where R.sup.14 is hydrogen or lower alkyl and the bond depicted as
in ring B is a double bond, or R.sup.13 is one or more atoms forming a ring system with rings B and D and the bond depicted as
in ring B is a single bond; at least one of R.sup.16, R.sup.17, and R.sup.18 is hydroxyl, oxygen, amino, alkyl amino, or NHR.sup.c where R.sup.c is as defined above; at least one of R.sup.19, R.sup.20 and R.sup.21 is oxygen, imino, iminium, lower alkyl iminium, or NHR.sup.c where R.sup.c is as defined above; in general formula VII, R.sup.6 is oxygen, imino, iminium, lower alkyl iminium, or NHR.sup.c where R.sup.c is as defined above, and in general formula VIII, R.sup.6 is hydrogen or NHR.sup.c where R.sup.c is as defined above. In particular embodiments, X.sup.1 is oxygen, R.sup.3, R.sup.4, R.sup.5, R.sup.8 (if present), R.sup.9, R.sup.12, R.sup.15, R.sup.17, R.sup.18, R.sup.19, R.sup.20, and R.sup.22 are hydrogen; R.sup.7 (if present) is hydrogen or halogen; R.sup.10 and R.sup.11 independently are hydrogen, amino, lower alkyl, carboxyl, or SO.sub.3H; R.sup.13 is hydrogen, lower alkyl, lower alkoxy, SO.sub.3H, or COOR.sup.14 where R.sup.14 is hydrogen or lower alkyl; R.sup.16 is hydroxyl, amino, lower alkyl iminium, or NHR.sup.c where R.sup.c is as defined above; R.sup.21 is oxygen, imino, iminium, or lower alkyl iminium; R.sup.6 is oxygen, imino, iminium, lower alkyl iminium, or NHR.sup.c where R.sup.c is as defined above in general formula VII, or R.sup.6 is hydrogen or NHR.sup.c where R.sup.c is as defined above in general formula VIII. In certain embodiments, if X.sup.1 is oxygen and either R.sup.16 or R.sup.18 is hydroxyl in structure VII, then R.sup.6 is other than oxygen, amino, or alkyl amino, or at least one of R.sup.3, R.sup.8 or R.sup.15 is other than hydrogen, or R.sup.13 is one or more atoms forming a ring system with rings B and D and the bond depicted as
in ring B is a single bond. Representative compounds according to general formulas VII and VIII are shown in Table 3.
(102) TABLE-US-00003 TABLE 3 Cpd R.sup.6* R.sup.7 R.sup.10 R.sup.11 R.sup.13 R.sup.16 R.sup.18 R.sup.19 R.sup.21 1a O H H H OCH.sub.3 OH H 1b O H CH.sub.3 H CH.sub.3 OH H 1c O H H COOH H OH H 1d O H H NH.sub.2 H OH H 1e O H H H SO.sub.3H OH H 1f O H SO.sub.3H H SO.sub.3H OH H 1g O F H H H OH H 1h NH H SO.sub.3H H SO.sub.3H NH.sub.2 H 1i N(CH.sub.3).sub.2.sup.+ H H H H OH H 1j N(CH.sub.3).sub.2.sup.+ H H H H N(CH.sub.3).sub.2 H 1k N(CH.sub.3).sub.2.sup.+ H SO.sub.3H H SO.sub.3H N(CH.sub.3).sub.2 H 6a H CH.sub.3 H CH.sub.3 OH H H O 6b H SO.sub.3H H SO.sub.3H OH H H O 6c H CH.sub.3 H CH.sub.3 NH.sub.2 H H NH 6d H CH.sub.3 H CH.sub.3 N(CH.sub.3).sub.2 H H N(CH.sub.3).sub.2.sup.+ 6e H H COOH H OH H H O 6f H H COOH H N(CH.sub.3).sub.2 H H N(CH.sub.3).sub.2.sup.+ 36a H H COOH CH.sub.3 H OH O H 36b H H H COOH H OH O H 36c H H COOH CH.sub.3 H OH N(CH.sub.3).sub.2.sup.+ H 36d H H H COOH H OH N(CH.sub.3).sub.2.sup.+ H *All R substituents not included in Table 3 are hydrogen and X.sup.1 is oxygen.
(103) In some embodiments, the NIR dyes have mixed annulation, e.g., type [a] and type [c], type [a] and type [b], or type [b] and type [c]. For example, in some embodiments, R.sup.3 and R.sup.4 together form an aryl or substituted aryl ring and R.sup.7 and R.sup.8 together form an aryl or substituted aryl ring as shown in general formula IX. In other embodiments, R.sup.3 and R.sup.4 together form an aryl or substituted aryl ring and R.sup.6 and R.sup.7 together form an aryl or substituted aryl ring as shown in general formula X. In still other embodiments, R.sup.1 and R.sup.2 together form an aryl or substituted aryl ring and R.sup.6 and R.sup.7 together form an aryl or substituted aryl ring as shown in general formula XI.
(104) ##STR00025##
In general formulas IX-XI, each bond depicted as is a single or double bond as needed to satisfy valence requirements. R.sup.1-R.sup.5, R.sup.7-R.sup.22 and X.sup.1 are defined as above, and R.sup.6 is hydrogen, hydroxyl, oxygen, halogen, thiol, amino, alkyl amino, or NHR.sup.c where R.sup.c is as defined above. In certain embodiments, X.sup.1 is oxygen; R.sup.1-R.sup.6 (if present), R.sup.8 (if present), R.sup.9, R.sup.10, R.sup.12, R.sup.15, R.sup.17, and R.sup.22 are hydrogen; R.sup.11 is hydrogen, amino, lower alkyl, carboxyl, or SO.sub.3H; R.sup.13 is hydrogen, lower alkyl, lower alkoxy, SO.sub.3H, COOR.sup.14 where R.sup.14 is hydrogen or lower alkyl and the bond depicted as
in ring B is a double bond, or R.sup.13 is one or more atoms forming a ring system with rings B and D and the bond depicted as
in ring B is a single bond; R.sup.16, R.sup.17, and R.sup.18 independently are hydrogen, hydroxyl, oxygen, lower alkoxy, amino, alkyl amino, or NHR.sup.c where R.sup.c is as defined above, and at least one of R.sup.16 and R.sup.18 is other than hydrogen; R.sup.19-R.sup.21 independently are hydrogen, hydroxyl, thiol, oxygen, imino, iminium, alkyl imino, alkyl iminium, amino, alkyl amino, or NHR.sup.c where R.sup.c is as defined above, and at least one of R.sup.19-R.sup.21 is other than hydrogen. In particular embodiments, X.sup.1 is oxygen; R.sup.1-R.sup.6 (if present), R.sup.8 (if present) R.sup.9, R.sup.10, R.sup.12, R.sup.15, R.sup.17, and R.sup.22 are hydrogen; R.sup.11 is hydrogen or carboxyl; R.sup.13 is lower alkyl or carboxyl; R.sup.16 and R.sup.18 independently are hydrogen, hydroxyl, or oxygen, or NHR.sup.c where R.sup.c is as defined above, and at least one of R.sup.16 and R.sup.18 is other than hydrogen; R.sup.19-R.sup.21 independently are hydrogen, oxygen alkyl iminium, or NHR.sup.c where R.sup.c is as defined above, and at least one of R.sup.19-R.sup.21 is other than hydrogen. Representative compounds according to general formulas IX-VI are shown in Table 4.
(105) TABLE-US-00004 TABLE 4 Cpd R.sup.11* R.sup.13 R.sup.16 R.sup.18 R.sup.19 R.sup.20 R.sup.21 33a COOH CH.sub.3 H OH H H O 33b H COOH H OH H H O 33c COOH CH.sub.3 H OH H H N(CH.sub.3).sub.2.sup.+ 33d H COOH H OH H H N(CH.sub.3).sub.2.sup.+ 35a COOH CH.sub.3 H OH O H H 35b H COOH H OH O H H 35c COOH CH.sub.3 H OH N(CH.sub.3).sub.2.sup.+ H H 35d H COOH H OH N(CH.sub.3).sub.2.sup.+ H H 37a COOH CH.sub.3 H OH H O H 37b H COOH H OH H O H 37c COOH CH.sub.3 H OH H N(CH.sub.3).sub.2.sup.+ H 37d H COOH H OH H N(CH.sub.3).sub.2.sup.+ H 38a COOH CH.sub.3 OH H H O H 38b H COOH OH H H O H 38c COOH CH.sub.3 OH H H N(CH.sub.3).sub.2.sup.+ H 38d H COOH OH H H N(CH.sub.3).sub.2.sup.+ H 39a COOH CH.sub.3 OH H O H H 39b H COOH OH H O H H 39c COOH CH.sub.3 OH H N(CH.sub.3).sub.2.sup.+ H H 39d H COOH OH H N(CH.sub.3).sub.2.sup.+ H H *All R substituents not included in Table 4 are hydrogen and X.sup.1 is oxygen.
(106) B. Structural Effects on Spectra and Stokes Shifts
(107) Systematic structure-based evaluation surprisingly demonstrated that shifting the position of a substituent on the annulated ring of a singly annulated xanthene substantially red-shifted the emission and absorption maxima. For example, in some embodiments as shown in
(108) Thus, the 3-1 transposition provides several advantages. Both absorbance and emission maxima are red-shifted. Because the emission spectra typically have a greater red shift than the absorbance spectra, the Stokes shift is enhanced. This behavior occurs across symmetric (e.g., general formula IV) and asymmetric structures (e.g., general formula III), and was observed across various ionizable groups (e.g., hydroxyl and amine functionalities). In some embodiments, combining the 3-1 transposition with transposition of ionizable functionalities also provides an unexpected pH response. Some rhodols absorb and emit long wavelengths at high pH. However, with some embodiments of the disclosed fluorophores that combine a 3-1 transposition with ionizable moiety transposition, long wavelength behavior was observed at acidic pH values.
(109) In some embodiments, anionic forms of the fluorophores having a 3-1 transposition produce a greater red shift than the corresponding neutral forms of the fluorophores. For example, the absorbance maximum of compound 15a is red-shifted 15 nm compared to compound 20a. However, the absorbance maximum of compound 15h, the anionic form of compound 15a, has a red-shift of 50 nm compared to compound 20c, the anionic form of compound 20a.
(110) There are through-space polar interactions between R.sup.18 and the internal bridge oxygen in compounds according to general formulas III, IX, and X. The field effect is further enhanced in the case of symmetric compounds, such as those according to general formula IV when R.sup.19 is oxygen. A unique intramolecular hydrogen-bonding network also is present in such compounds, e.g., compound 8 (
(111) In some embodiments, e.g., compound 8, NIR fluorescence was observed in compounds exhibiting hydrogen bonding up to pH 9, while removal of the shared proton was observed to quench the fluorescence. Notably, compound 7 exists as its corresponding colorless lactone in 1:9 DMSO:buffer below physiological pH of 7.4, and thus embodies a unique pH probe.
(112) Some embodiments of fluorophores having a chemical structure according to general formula III or IV have longer absorption and emission wavelengths than rhodamine. Analogues having an NHR.sup.c (boronic acid) substituent at R.sup.16 and R.sup.6 (formula III) or R.sup.21 (formula IV) are further red-shifted that the corresponding fluorophores having an NH.sub.2 at R.sup.16 and R.sup.6/R.sup.21. In the absence of sugars, the boronic acids may be quenched through a PET (photoinduced electron transfer) mechanism. In certain embodiments, bathochromic shifts and increased quantum yields were observed for both asymmetric (formula III) and symmetric (formula IV) bis-boronic acid analogues upon sugar binding.
(113) IV. SYNTHESIS
(114) Some embodiments of naphthofluorescein analogues having a chemical structure according to general formula IV are synthesized as shown in Scheme 1 (
(115) Analogues having a chemical structure according to general formula III are synthesized by acid condensation as shown in Scheme 2 (
(116) Certain asymmetric seminaphthofluorescein and rhodamine analogues having a chemical structure according to general formula III are synthesized as shown in Scheme 3 (
(117) A commercially available, lactone-containing naphthofluorescein analogue was converted to its methyl ester via Fischer esterification as shown in Scheme 4 (
(118) Some analogues having chemical structures according to general formula VI and some asymmetric, extended-conjugation analogues having chemical structures according to general formulas VIII-XI are synthesized as shown in Scheme 5 (
(119) Some analogues having chemical structures according to general formula V and VII are synthesized as shown in Scheme 6 (
(120) Some analogues having chemical structures according to general formula I where X.sup.1 is Si(CH.sub.3).sub.2, Ge(CH.sub.3).sub.2, or Sn(CH.sub.3).sub.2 may be synthesized according to Scheme 7 (
(121) Some analogues having chemical structures according to general formula I where X.sup.1 is S or Se may be synthesized according to Scheme 8 (
(122) Some analogues having chemical structures according to general formula (III) where R.sup.6 and R.sup.16 are amino or boronic acid are synthesized according to Scheme 9 (
(123) Some analogues having chemical structures according to general formula (iv) where R.sup.16 and R.sup.21 are amino or boronic acid are synthesized according to Scheme 10 (
(124) In some embodiments, it may be advantageous to reduce the dye pKa and/or to increase aqueous solubility of the dyes. In certain embodiments, pKa may be reduced by halogenating the dye, such as by position one or more fluorine atoms ortho to one or more ionizable moieties such that a majority of the dye molecules are in an ionic form in a neutral aqueous solution. The inventors have discovered that ionized species of the disclosed dyes generally have a larger Stokes shift than neutral dye molecules. Embodiments of the dyes may be fluorinated by using a fluorinated naphthol during the synthesis, i.e., during the condensation reaction. Fluorinated naphthols may be synthesized by reacting hydroxynaphthalenes with a fluorine donor (e.g., Selectfluor (1-chloromethyl-4-fluoro-1,4-diazoniabicyclo[2.2.2]octane bis(tetrafluoroborate)) in methyl cyanide at reflux as described in the literature (Yang et al., Heteroatom Chem., 1998, 9, 229-239; Bluck et al., J. Fluor. Chem., 2004, 125, 1873-1877).
(125) In some embodiments, it also may be advantageous to reduce non-specific binding in biological media. To reduce non-specific binding, oligopegylated derivatives of the disclosed dyes can be prepared by conjugating the dye to an oligoethylene glycol. If bi-functional pegylating reagents are used, the dye may further be conjugated to other biological molecules of interest. Suitable bi-functional pegylating reagents comprising 6-8 ethylene glycol units are commercially available or can be prepared using literature protocols (Svedhem et al., J. Org. Chem., 2001, 66, 4494-4503; Wosnick et al., J. Am. Chem. Soc., 2005, 127, 3400-3405). Pegylated reagents can be conjugated via amide bond formation to embodiments of the disclosed dyes that comprise a carboxylic group. Pegylation also may increase solubility of dyes having hydrophobic properties.
(126) V. APPLICATIONS
(127) Some embodiments of disclosed NIR dyes are suitable for use in challenging biological media such as blood, plasma, and urine. Blood has strong background absorption, significant autofluorescence, and scatter in the visible region. Certain embodiments of the disclosed NIR dyes have emission spectra maxima at wavelengths that are long enough to overcome interference from blood hemoglobin, e.g., an emission spectrum maximum at greater than 640 nm, or greater than 680 nm. In particular embodiments, the disclosed NIR dyes also have an absorbance spectrum maximum that is greater than 640 nm, such as greater than 680 nm. In such embodiments, a light source having a wavelength greater than 640 nm can be utilized, thereby minimizing light absorption by blood. Dyes that function beyond the optical range of blood will simplify testing for analytes and biomarkers by limiting dilution and sample preparation/handling, thereby reducing sources of error, reducing time to obtain results, and/or reducing health hazards due to sample handling and manipulation. Current methods and detection agents utilize chromatography, fragile materials (e.g., those that are unstable in aqueous solution, require storage below 20 C. and/or require storage in the dark), and/or a high degree of sample processing. For example, in current methods, samples may be diluted more than 1000-fold to overcome optical interference from blood, or testing may be performed in plasma instead of whole blood. In contrast, some embodiments of the disclosed NIR dyes are stable for weeks at ambient temperature, even in solution. Certain embodiments of the disclosed NIR dyes also are more photostable in cell culture media than fluorescein.
(128) In particular, some embodiments of the disclosed NIR dyes include functional groups that may facilitate detection of specific molecular biomarkers. Embodiments of the NIR dyes may be functionalized to (a) produce a desired geometry having a combination of covalent and/or supramolecular interactions between the dye and a desired biomarker and/or (b) to alter oxidation-reduction and/or energy transfer properties in the presence of a desired biomarker. For instance, molecular modeling also indicates that some embodiments having a chemical structure according to general formula VI and polar end groups such as amino, oxygen, and/or hydroxyl groups at positions R.sup.17 and R.sup.20 may exhibit favorable electrostatic interactions with the polar ends of glutathione (
(129) Molecular modeling also indicates that some embodiments of the NIR fluorophores having a chemical structure according to general formula VI wherein at least one of R.sup.17 and R.sup.20 is NHR.sup.c where R.sup.c is
(130) ##STR00026##
may preferentially interact with specific nucleosides (
(131) Biomarkers of interest include glutathione (GSH), which is diminished in whole blood, leukocytes and plasma in mitochondrial disorders (e.g., diabetes, atherosclerosis, neurodegenerative diseases, hypoxic-ischemic encephalopathy, autism, retinopathy of prematurity, chronic progressive external ophthalmoplegia, and cancer (Akturi et al., PNAS U.S.A., 2009, 106, 3941-3945)) and organic acidemias (e.g., methylmalonic acidemia). Some commercially available methods for detecting GSH utilize detection agents that are not selective for GSH over other thiols (e.g., cysteine, dithiothreitol) and/or require testing in plasma. However, GSH levels in whole blood are in the millimolar range, or 100-1000 fold higher than in plasma.
(132) Embodiments of the disclosed NIR dyes may be suitable for selective detection of GSH in blood. For example, molecular modeling shows that compound 5a (Table 2) has very favorable structural interactions with GSH. Additionally, compound 5a was found to be detectable in a 5% blood solution (see Example 2). Embodiments of NIR dyes functionalized to promote multipoint, glutathione-selective covalent, supramolecular and/or redox interactions may serve as indicators for oxidative stress and mitochondrial disorders.
(133) Rhodamine B lactol has GSH selectivity, which is thought to arise from reaction with the aldehyde tautomer, thereby affording the observed emission and absorbance increases.
(134) ##STR00027##
It is believed that the equilibrium favors the thiohemiacetal in the case of GSH due, at least in part, to favorable electrostatic interactions between the polar groups of the dye (which actually modulate the ionization state and optical properties) and those of the GSH tripeptide. In order to understand potential salt bridge formation and ion pairing, the covalent complexes formed in the reaction of dyes such as compound 24 were simulated, demonstrating that the relatively extended GSH moiety can interact with the polar ends of the dyes simultaneously (
(135) Other biomarkers of interest include succinyl-5-amino-4-imidazole-carboxamide riboside (SAICA-riboside) and succinyladenosine (S-Ado), which are indicators of adenylosuccinate lyase (ADSL) deficiency.
(136) ##STR00028##
ADSL deficiency is a rare (approximately 1 in 200,000) inborn error of purine metabolism, which can result in mental retardation and seizure (Jaeken, Lancet, 1984, 2, 1058-1061). Undiagnosed genetic defects in purine and pyrimidine (PP) metabolism may result in early death and/or institutionalization. ADSL deficiency is characterized by massive urinary excretion (millimolar levels) of SAICA-riboside and S-Ado, the nucleosides corresponding to SAICA-ribotide (SAICAr) and adenylosuccinate.
(137) Embodiments of the disclosed dyes functionalized with covalent and supramolecular binding sites targeted for specific nucleosides may serve as indicators for ADLS deficiency. In some embodiments, the NIR dye includes at least one boronic acid functional group, i.e., at least one R group is NHR.sup.c where R.sup.c is
(138) ##STR00029##
(139) A rhodamine modified with a phenyl boronic acid can exhibit unprecedented affinity for ribose and congeners as compared to fructose (Jiang et al., J. Am. Chem. Soc., 2006, 128, 12221-12228).
(140) ##STR00030##
Computer-assisted molecular simulations indicate that, apart from the preference of the boronic acid to react with the 2,3-cis diol of the furanose form of ribose and the strong electrostatic interaction BOHN.sup.+, non-covalent secondary interactions play an important role modifying the ionization state of the chromophore.
(141) In some instances, if evaluation in biological media is unsatisfactory, a molecule-imprinted polymer (MIP) specific to a particular analyte may be synthesized and utilized in a solid-phase extraction step prior to evaluation with embodiments of the disclosed NIR dyes. The MIP will allow capture and concentration of the analyte. Excellent synergism may be achieved as any deficiency in selectivity by the fluorophore-viologen conjugates and/or the MIP may compensate for the other component.
(142) Some embodiments of the disclosed NIR dyes may be useful for cell imaging, intracellular pH sensing (e.g., by evaluating the absorbance and/or emission spectra and identifying dye ionization states within the cell), and/or detection of intracellular analytes such as thiols, hydrogen peroxide, hydrogen sulfide, and/or cyanide.
(143) VI. KITS
(144) Kits are also a feature of this disclosure. Embodiments of the kits include at least one compound according to any one of general formulas I-XI and suitable for selectively detecting an analyte in a sample (e.g., a biological fluid such as blood or urine). In some embodiments, the kits also include at least one buffer solution in which the compound, when combined with a sample including, or suspected of including, an analyte, will undergo a change in its absorbance spectrum and/or emission spectrum compared to the compound in the buffer solution combined with a sample that does not include the analyte. The kits may include a color comparison chart for evaluating a color change produced by a reaction between the compound and the analyte. The kits also may include one or more containers, such as a disposable test tube or cuvette, in which the detection can be performed. The kits may further include instructions for performing the detection. In some embodiments, the kits include control samples of analytes, e.g., glutathione, cysteine, homocysteine, succinyl-5-amino-4-imidazolecarboxamide riboside, and/or succinyladenosine. Typically the control samples are provided in solid form.
(145) In some embodiments of the kits, the compound is provided as a solid, and the buffer is provided in liquid form. The buffer may be provided at a concentration suitable for detecting Cys, Hcy, GSH, succinyl-5-amino-4-imidazolecarboxamide riboside, succinyladenosine or a mixture thereof. Alternatively, the buffer may be provided as a concentrated solution, which is subsequently diluted prior to use. In certain embodiments, the compound may be premeasured into one or more containers (e.g., test tubes or cuvettes), and the detection is subsequently performed by adding the buffer and test sample to the container.
(146) VII. EXAMPLES
(147) Reagents and General Procedures:
(148) Chemistry. Unless otherwise indicated, all commercially available starting materials were used directly without further purification. Naphthofluorescein was obtained from Sigma-Aldrich. Silica gel (Sorbent Technologies) 32-63 m was used for flash column chromatography. .sup.1H-NMR was obtained on an ARX-400 Advance Bruker spectrometer. Chemical shifts () are given in ppm relative to d.sub.6-DMSO (2.50 ppm, .sup.1H, 39.52 .sup.13C) unless otherwise indicated. MS (HRMS, ESI) spectra were obtained at the Portland State University Bioanalytical Mass Spectrometry Facility on a ThermoElectron LTQ-Orbitrap high resolution mass spectrometer with a dedicated Accela HPLC system. General acid condensation using methanesulfonic acid. Dihydroxynaphtahlene (3.12 mmol) and phthalic anhydride (1.56 mmol) are dissolved in 3 mL of methanesulfonic acid. The mixture is stirred at 90 C. for 24 hours. The mixture is allowed to cool down to room temperature, then poured into distilled water (50 mL). The precipitate is filtered and washed with water (350 mL). If no precipitate is formed, the mixture is neutralized to pH 5-7 by portion-wise addition of solid NaHCO.sub.3. The precipitate is dried under vacuum. The target compound is isolated by flash column chromatography on silica gel. General condensation method using CH.sub.3SO.sub.3H:TFA 1:1 mixture. Hydroxybenzophenone (918 mol) and 1,8-naphthalene derivative (1380 mol) are dissolved in 1.5 mL of methanesulfonic acid, then 1.5 mL of trifluoroacetic acid (TFA) are added. The mixture is heated and stirred at 80 C. for 16-24 hours. The reaction mixture is allowed to warm to room temperature, and then poured into 50 mL of deionized (DI) water. The mixture is neutralized to pH 6-7 by portion-wise addition of solid NaHCO.sub.3. The resulting precipitate is filtered, washed with DI water and air dried. The target compound is isolated by flash column chromatography on silica gel. General esterification method A. Carboxylate (0.243 mol) is dissolved in 2 mL of methanol (MeOH). To this solution is added concentrated H.sub.2SO.sub.4 (100 L) dropwise, then the mixture is refluxed 24 hours. The mixture is allowed to cool down to room temperature, then poured into 50 mL of ice water and 200 mg of NaHCO.sub.3 is added in one portion. If a precipitate forms, the solid is filtered and washed with 2% NaHCO.sub.3 (210 mL), then with water (210 mL). If no precipitate is obtained, the neutralized aqueous phase is extracted with CHCl.sub.3 (350 mL). The organic phase is dried over Na.sub.2SO.sub.4 and the solvent evaporated under vacuum. The target compound is then isolated by flash column chromatography. General esterification method B. Under an argon atmosphere, the compound (0.131 mmol) is dissolved in 25 mL of anhydrous methanol. The solution is cooled to 0 C. in an ice bath. Acetyl chloride (750 L) is added dropwise. The mixture is stirred and kept at 50 C. for 48 hours. Acetyl chloride (300 L) is added dropwise, and the mixture kept at 50 C. for additional 24 hours. The mixture is allowed to cool down to room temperature, and the solvent is evaporated under vacuum.
Example 1
Syntheses and Characterization
(149) As shown in Scheme 1, analogues having a structure according to general formula IV were synthesized either via acid condensation of 1,8-dihydroxy-naphthalene and phthalic anhydride in methanesulfonic acid or by condensation of 1,8-dihydroxynaphthalene with the corresponding aldehydes in 85% H.sub.3PO.sub.4 at 125 C. /24 hours. The naphthofluorescein methyl ester 22 was obtained via a typical Fischer esterification protocol from 21 (Scheme 4). Asymmetric seminaphthofluorescein, rhodol and rhodamine analogues according to general formula III (Schemes 2 and 3) were synthesized by acid condensation of hydroxybenzophenones with the corresponding naphthols in a mixture of CH.sub.3SO.sub.3H:TFA 1:1 at 80 C. for 16-24 hours. The hydroxybenzophenones and 1,8-naphthalene derivatives required were synthesized according to described or modified literature protocols. The methyl ester derivatives were obtained by esterification in MeOH catalyzed by either H.sub.2SO.sub.4 or HCl; further methyl alkylation was furnished by treatment of either the carboxylate or methyl ester intermediate with methyl iodide in the presence of K.sub.2CO.sub.3 in dimethylformamide. In general, overall good yields were obtained for most of the compounds included in this series with the exception of the condensation products between dihydroxybenzophenone and 8-amino naphthol derivatives, where the major isolated product corresponds to fluorescein. All compounds were isolated by flash column chromatography (normal or reversed phase) and characterized by NMR and MS. The structure of compound 14a (
(150) Compounds 21 and 7 (type [c] fully annulated naphthofluorescein with known regiochemistry versus fully annulated type [c] naphthofluorescein with 3-1 transposition)
(151) Compounds 21 and 7 were characterized by UV-visible spectroscopy (
(152) Compounds 21 and 7 also were characterized by their fluorescence emission spectra. Fluorescence was measured using 15 M solutions of each compound in a solution that was 90% aqueous, 10% DMSO. For compound 21, the longest wavelength emission observed from the dianions occurred near 680 nm at pH 9. For compound 7 (transposed), the longest wavelength emission observed from the monoanion occurred near 800 nm at pH 9. Emission from the transposed dianion in sodium hydroxide was either too weak to be detected or, more likely, beyond the working range of the instrument (>850 nm). The 3-1 transposition resulted in red-shifted emission of about 120 nm, and possibly more depending on the ionization state.
(153) The Stokes shift of each compound was measured and determined to be about 80 nm for the compound 21 dianion, and about 150 nm for the compound 7 (transposed) monoanion. Thus, the 3-1 transposition also enhanced the Stokes shift.
(154) Compounds 22 and 8 (type [c] fully annulated naphthofluorescein methyl ester with known regiochemistry versus fully annulated type [c] naphthofluorescein methyl ester with 3-1 transposition)
(155) Compound 22 was synthesized according to Scheme 4. Naphthofluorescein (compound 21, 0.05 g, 0.115 mmol) was dissolved in 2 mL methanol. The solution was cooled to 0 C. in an ice bath. Concentrated sulfuric acid (100 L) was added to the solution, and the mixture was refluxed for 24 hours. The mixture was allowed to cool to room temperature, and then poured into 250 mL of ice water; 200 mg NaHCO.sub.3 was added. The precipitate was filtered and washed twice with 2% NaHCO.sub.3 and then twice with water. Compound 22 was isolated by flash column chromatography on silica gel using CH.sub.2Cl.sub.2:MeOH 9:1 for elution. The yield was 26 mg, 50%. .sup.1H NMR (400 MHz, DMSO) 10.05 (s, 1H), 8.96 (d, J-9.2 Hz, 2H), 8.60 (d, J-9.0 Hz, 1H), 8.33 (d, J=7.9 Hz, 1H), 7.98 (t, J=6.9 Hz, 1H), 7.89 (t, J=7.7 Hz, 1H), 7.63 (d, J=9.2 Hz, 2H), 7.56 (d, J=6.8 Hz, 1H), 7.32-7.21 (m, 1H), 7.18-7.03 (m, 2H), 6.91 (d, J=9.1 Hz, 2H), 3.56 (s, 3H). HR ESI [M+H.sup.+] m/z 447.1231 calc for C.sub.29H.sub.19O.sub.5; 447.1237.
(156) Compound 8 was synthesized by dissolving 1,8-dihydroxy-naphthalene (0.5 g, 3.12 mmol) and phthalic anhydride (0.231 g, 1.56 mmol) in 2 mL of methanesulfonic acid, and then adding 2 mL trifluoroacetic acid. The mixture was heated and stirred at 80 C. for 24 h. The mixture was allowed to cool to room temperature, and then poured into 100 mL of deionized water. The precipitate (compound 7) was filtered and washed with deionized water, and then isolated by flash column chromatography on silica gel using CH.sub.2Cl.sub.2:MeOH 9:1 for elution. Yield 0.122 g, 18%. Esterification then was carried out as described for compound 22. The target compound was isolated by flash column chromatography on silica gel using CH.sub.2Cl.sub.2:MeOH 9:1 for elution. Yield 0.023 g, 86%. .sup.1H NMR (400 MHz, DMSO) 14.45 (s, 1H), 8.32 (dd, J=7.9, 1.0 Hz, 1H), 7.97 (td, J=7.5, 1.3 Hz, 1H), 7.87 (td, J=7.7, 1.3 Hz, 1H), 7.62-7.66 (m, 4H), 7.56 (dd, J=7.6, 0.9 Hz, 1H), 7.13, (d, J=7.2 Hz, 2H), 6.91 (dd, J=8.4, 0.8 Hz, 2H), 6.77 (d, J=9.1 Hz, 2H), 3.55 (s, 3H). .sup.13C NMR (101 MHz, DMSO) 167.48, 165.22, 154.58, 137.69, 135.10, 134.52, 133.66, 130.95, 130.56, 130.40, 130.05, 129.20, 120.79, 119.99, 119.87, 116.26, 112.80, 52.42. HR ESI [M+H.sup.+] m/z 447.1236, calc for C.sub.29H.sub.19O.sub.5; 447.1237.
(157) Compounds 22 and 8 were characterized by UV-visible spectroscopy (
(158) It is interesting to note that for the transposed compound, ionization of the hydroxyl leads to shorter wavelength absorption. This is counter to what is observed for the known regiochemistry. It is also apparent from these first two examples that the 3-1 transposition's effect on spectral properties (red shifted spectra) is more pronounced when the carboxylate is replaced by a methyl ester.
(159) The unique location of hydroxyl groups provided by the 3-1 transposition leads to increased interaction with the xanthene oxygen. This is one possible explanation for the observed red shift and unexpected behavior. Molecular modeling has demonstrated that in the neutral form, the hydroxyl proton of the transposed structure is held tightly with two hydrogen bonds and that in the anionic form, the xanthene oxygen is more electropositive for the transposed vs. the known regiochemistry (Mulliken charge of 0.289 vs. 0.386). Work is currently ongoing to further characterize this interaction and employ this strategy to further tune the spectral properties of xanthene-based structures without the need for complicated synthetic strategies which can include replacing the xanthene oxygen with more electropositive elements (i.e. S, Se, C, Si, etc.). In recent work Nagano and co-workers have used a similar strategy to extend the absorption and emission wavelength of rhodamines and pyronines by approximately 80 nm each (DOI: 10.1021/cb1002416). It should be noted that this approach retains the relatively small Stokes shifts (15-20 nm) present in the parent structure. However, the transposition approach disclosed herein increased the Stokes shift. Additionally, the emission of compounds reported by Nagano and co-workers are ca. 100 nm or more to the blue of those resulting from the 3-1 transposition approach described herein.
(160) Compounds 22 and 8 also were characterized by their fluorescence emission spectra. Fluorescence was measured using 15 M solutions of each compound in a solution that was 90% aqueous, 10% DMSO For compound 22, the longest wavelength emission observed from the anion occurred near 690 nm at pH 9. For compound 8 (transposed), emission was not observed. Given the greater than 800 nm absorbance peak and the typically large Stokes shifts observed in this class of compounds, it is likely that the emission was beyond the working range of the instrument (>850 nm). Assuming that emission of the transposed compound is beyond 850 nm, the 3-1 transposition resulted in red-shifted emission of more than 160 nm.
(161) The Stokes shift of each compound was measured and determined to be about 80 nm for the compound 22 anion, and unmeasurable for the compound 8 (transposed) neutral form. It is likely that the 3-1 transposition also enhanced the Stokes shift.
(162) Absorbance and fluorescence of compounds 22 and 8 also were measured in methanol. Compound 22 exhibited an absorption maximum at 598 nm. Compound 8 exhibited an absorption maximum at 701 nm. Compound 22 exhibited an emission maximum at 688 nm. Compound 8 exhibited an emission maximum at 816 nm. Thus, placement of the hydroxyl group proximal to the xanthene internal oxygen in compound 8 produced a bathochromic shift of 103 nm in the absorption spectrum. The transposition also imparted a corresponding shift in the excitation spectrum, and a large bathochromic shift of 128 nm in the fluorescence emission. Importantly, compound 8 absorbs and emits in the near-infrared with absorption and emission maxima of 701 nm and 816 nm, respectively. Analogous trends were observed in other solvent systems, e.g., DMSO, DMSO:aqueous 1:1 (pH 9), DMSO: aqueous 1:9 (pH9), DMSO: aqueous 1:1 (pH 12.1, NaOH), and DMSO: aqueous 1:9 (pH 12.1, NaOH).
(163) Compounds 20a and 15a (type [c] annulated seminaphthofluorescein methyl ester with known regiochemistry versus annulated type [c] seminaphthofluorescein methyl ester with 3-1 transposition)
(164) Some type [c] Seminaphthofluoresceins with the regiochemistry of the ionizable group on the carbon 3 have been commercially available. However, given the large effect observed when the carboxylate was replaced with a methyl ester on compounds with the transposed geometry above, the previously unreported seminaphthofluorescein methyl ester analogue was prepared for direct comparison with new transposed compounds.
(165) Compound 20a was synthesized (Scheme 3) by condensing compound 17a (0.25 g, 0.968 mmol) and compound 18a (0.232 g, 1.45 mmol) under acidic conditions. Compound 19a was isolated by flash column chromatography on silica gel using EtOAc:MeOH 9:1 for elution. Yield 303 mg, 82%. Compound 19a (50 mg, 131 mol) was esterified as described previously for compound 22. Compound 20a was isolated by flash column chromatography on silica gel using EtOAc:MeOH 9:1 for elution. Yield 27 mg, 52%. .sup.1H NMR (600 MHz, DMSO) 10.57 (s, 1H), 8.53 (d, J=9.1 Hz, 1H), 8.25 (dd, J=8.0, 1.0 Hz, 1H), 7.90 (td, J=7.5, 1.3 Hz, 1H), 7.81 (td, J=7.8, 1.3 Hz, 1H), 7.53 (dd, J=14.7, 7.7 Hz, 2H), 7.34 (dd, J=9.1, 2.4 Hz, 1H), 7.23 (d, J=2.3 Hz, 1H), 6.86 (d, J=9.6 Hz, 1H), 6.78 (d, J=89 Hz, 1H), 6.50 (D, J=2.0 Hz, 1H), 6.47 (dd, J=9.6, 2.0 Hz, 1H), 3.56 (s, 3H). .sup.13C NMR (151 MHz, DMSO) 183.52, 165.24, 159.27, 158.26, 150.81, 149.36, 137.52, 134.26, 133.27, 130.72, 130.69, 130.04, 192.92, 129.85, 129.48, 124.47, 123.27, 122.90, 119.85, 117.45, 116.08, 113.91, 110.07, 104.55, 52.10. HR ESI [M+H.sup.+] m/z 397.1068; calc for C.sub.25H.sub.17O.sub.5; 397.1081.
(166) Compound 15a was synthesized (Scheme 2) by condensing compound 12a (0.250 mg, 0918 mmol) and compound 13a (0.232 g, 1.45 mmol) under acidic conditions. Compound 14a was isolated by flash column chromatography on silica gel using CH.sub.2Cl.sub.2:MeOH 9:1 for elution. Yield 300 mg, 81%. Compound 14a was esterified as described previously for compound 22. Compound 15a was isolated by flash column chromatography on silica gel using CH.sub.2Cl.sub.2:MeOH 9:1 for elution. Yield 40 mg, 77%. .sup.1H NMR (600 MHz, DMSO) 10.78 (s, 1H), 8.25 (d, J=7.9 Hz, 1H), 7.91 (td, J=7.6, 1.3 Hz, 1H), 7.86-7.77 (m, 1H), 7.63-7.51 (m, 3H), 7.40 (s, 1H), 7.13 (s, 1H), 6.86 (d, J=9.7 Hz, 1H), 6.81 (d, J=8.7 Hz, 1H), 6.50 (dd, J=9.7, 1.9 Hz, 1H), 6.34 (d, J=2.0 Hz, 1H), 3.55 (s, 3H). HR ESI [M+H.sup.+]m/z 397.1079; calc for C.sub.25H.sub.17O.sub.5; 397.1081.
(167) Compounds 20a and 15a were characterized by UV-visible spectroscopy (
(168) Compounds 20 and 15a also were characterized by their fluorescence emission spectra. Fluorescence was measured using 15 M solutions of each compound in a solution that was 90% aqueous, 10% DMSO. For compound 20a, the longest wavelength emission observed from its corresponding anion, compound 20c, occurred near 640 nm in NaOH (pH 12). For compound 15a (transposed), the longest wavelength emission observed from the corresponding anion, compound 15h, occurred near 760 nm in NaOH. The 3-1 transposition resulted in red-shifted emission by about 120 nm for the longest wavelength-emitting species.
(169) The Stokes shift of each compound was measured and determined to be about 95 nm for the compound 20c anion, and about 165 nm for the compound 15h (transposed) anion. Thus, the 3-1 transposition enhanced the Stokes shift by about 70 nm.
(170) Absorbance and fluorescence of compounds 20a and 15a also were measured in methanol. Compound 20a exhibited absorption maxima at 487 nm and 521 nm. Compound 15a exhibited absorption maxima at 501 nm and 536 nm. Compound 20a exhibited an emission maximum at 550 nm. Compound 15a exhibited an emission maximum at 582 nm. Thus, placement of the hydroxyl group proximal to the xanthene internal oxygen in compound 15a produced a bathochromic shift of 15 nm in the absorbance spectrum. The transposition also imparted a corresponding shift in the excitation spectrum, and a large bathochromic shift of 32 nm in the fluorescence emission.
(171) Although the red shift of compound 15a in methanol was modest, its corresponding anion (compoundl5h) in DMSO:aqueous base 1:9 was shifted more substantially by 50 nm to 599 nm as compared to anion 20c under the same conditions. Its fluorescence emission was shifted by an even larger 130 nm, emitting at 760 nm. The comparatively large shift of the anion 15h supports a polar effect as playing a key role in modulating the HOMO-LUMO gap in the compound. The oxoanion of asymmetric 15h is expected to have a more significant field effect on the polarity of the proximal ether oxygen as compared to the corresponding oxoanion 20c. Like many other NIR-emitting compounds, compound 15h displays a relatively low quantum yield (less than 1%) in aqueous solution. The brightness of compound 15h is comparable to other NIR probes.
(172) Compounds 15b and 15d (type [c] annulated seminaphthorhodafluor methyl esters (free amino and dimethyl, respectively) with 3-1 transposed regiochemistries)
(173) The methyl ester analogue of known seminaphthofluorescein behaves similarly to previously reported compounds. Only a slight red shift, on the order of approximately 10 nm as compared to the carboxylate containing compound (Whitaker et al. Analytical Biochemistry, 1991, 194, 330-344), was observed. Thus the methyl ester analogues of known seminaphthorhodafluors (alternatively known as seminaphthorhodols) were not prepared for direct comparison. Literature values for similar compounds with the known regiochemistry were used for comparison with compounds 15b and 15d.
(174) Compound 15b (free amino) was characterized by UV-visible spectroscopy (
(175) Compound 15b also was characterized by its fluorescence emission spectrum and compared to C.SNARF-5. Fluorescence was measured using a 15 M solution of compound 15b in a solution that was 90% aqueous, 10% DMSO. For C.SNARF-5, the longest wavelength emission observed from the anion occurred near 632 nm (Whitaker et al., Anal. Biochem. 1991, 194, 330-344). For compound 15b (transposed), the longest wavelength emission observed from the phenoxide occurred near 770 nm. The 3-1 transposition resulted in red-shifted emission by about 138 nm for the longest wavelength-emitting species.
(176) The Stokes shift of each compound was measured and determined to be about 82 nm for the C.SNARF-5 anion, and about 155 nm for the compound 15b (transposed) phenoxide. Thus, the 3-1 transposition enhanced the Stokes shift by about 73 nm.
(177) Compound 15d (dimethyl) was characterized by UV-visible spectroscopy (
(178) Compound 15d also was characterized by its fluorescence emission spectrum and compared to SNARF-1. Fluorescence was measured using a 15 M solution of compound 15d in a solution that was 90% aqueous, 10% DMSO. For SNARF-1, the longest wavelength emission observed from the anion occurred near 631 nm (Whitaker et al., Anal. Biochem. 1991, 194, 330-344). For compound 15d (transposed), the longest wavelength emission observed from the phenoxide occurred near 780 nm (NaOH, pH 12). The 3-1 transposition resulted in red-shifted emission by about 149 nm for the longest wavelength-emitting species.
(179) The Stokes shift of each compound was measured and determined to be about 58 nm for the SNARF-1 anion, and about 150 nm for the compound 15d (transposed) phenoxide. Thus, the 3-1 transposition enhanced the Stokes shift by about 92 nm.
(180) Compounds 20b and 15g (type [c] annulated seminaphthorhodamine methyl esters (free amino) with known and 3-1 transposed regiochemistries, respectively)
(181) To the best of the inventors' knowledge, the full seminaphthorhodamine analog with type [c] annulation and the known regiochemistry has not been reported. We prepared this compound (20b) for direct comparison with a new seminaphthorhodamine with the 3-1 transposition (15g).
(182) Compounds 20b and 15g were characterized by UV-visible spectroscopy (
(183) Compounds 20b and 15g also were characterized by their fluorescence emission spectra. Fluorescence was measured using 15 M solutions of each compound in a solution that was 90% aqueous, 10% DMSO. For compound 20b, the longest wavelength emission observed from the cation occurred near 640 nm at pH 7.4. For compound 15g (transposed), the longest wavelength emission observed from the cation occurred near 770 nm at pH 7.4. The 3-1 transposition resulted in red-shifted emission by about 130 nm.
(184) The Stokes shift of each compound was measured and determined to be about 100 nm for the compound 20b cation, and about 200 nm for the compound 15g (transposed) cation. Thus, the 3-1 transposition enhanced the Stokes shift by about 100 nm.
(185) Compounds 15e and 15f (Type [c] annulated Seminaphthorhodafluor methyl esters (free amino and dimethyl) with 3-1 transposed regiochemistries and the ionizable moieties transposed)
(186) In addition to the previous examples, a second series of seminaphthorhodafluors (15e-15f) with the 3-1 transposition was prepared in which the ionizable hydroxyl and amine (free amino and dimethyl) functionalities also were transposed. To the best of the inventors' knowledge, no directly comparable structure has been reported in the literature. The nearest compound for comparison, a type [c] annulated seminaphthorhodafluor with the known regiochemistry, was reported to display longer wavelength fluorescence, emitting at 600 nm in MeOH when excited at 525 nm. (Clark et al., Tetrahedron Lett., 2004, 45, 7129-7131.)
(187) The new series of compounds based on transposition of the ionizable hydroxyl and amine functionalities combined with the 3-1 transposed regiochemistry disclosed herein displays unanticipated acid-base properties. Known seminaphthorhodafluors display long wavelength absorption at high pH; however, the free amino compound in this series respond to low pH with increased long wavelength absorption.
(188) Preliminary data also indicate that this series (free amino and dimethyl) exhibit red-shifted spectra comparable to others compounds with the 3-1 transposition disclosed herein. Emission maxima of the longest wavelength emitting species are in the range of 740-780 nm. It is apparent the red-shifted compounds based on the combination of transpositions are considerably more red shifted as compared to the previously reported compounds.
(189) Compound 10-7-(2,5-dimethylphenyl)-13-hydroxy-1H-dibenzo[c,h]xanthen-1-one
(190) ##STR00031##
(191) 1,8-dihydroxynaphthalene (0.298 mg, 1.86 mmol) and 2,5-dimethyl-benzaldehyde were suspended in 2 mL of 85% H.sub.3PO.sub.4, the mixture was vigorously stirred and heated at 125 C. for 24 hours. The mixture was allowed to cool down to room temperature and then poured into 50 mL of water. The precipitate formed was filtered and washed with water (250 mL). The target compound is isolated by flash column chromatography on silica gel using CH.sub.2Cl.sub.2:MeOH 9:1 for elution. Yield 67 mg, 17%. .sup.1H NMR (400 MHz, CDCl.sub.3) 7.59-7.45 (m, 4H), 7.35 (s, 2H), 7.16 (s, 2H), 7.10 (s, 2H), 7.04 (d, J=6.8 Hz, 2H), 6.94 (d, J=8.9 Hz, 2H), 2.45 (s, 3H), 2.00 (s, 3H). .sup.13C NMR (101 MHz, CDCl.sub.3) 155.96, 138.17, 136.37, 135.49, 133.06, 132.79, 130.87, 130.65, 129.51, 121.04, 120.73, 120.52, 117.21, 113.46, 21.18, 19.43. HR ESI [M+H.sub.+] m/z 417.1476, calc for C.sub.29H.sub.21O.sub.3; 417.1496.
(192) Compound 16a-methyl 2-(1-methoxy-10-oxo-10H-benzo[c]xanthen-7-yl)benzoate
(193) ##STR00032##
(194) Under argon atmosphere, compound 14a (0.050 g, 0.131 mmol) and K.sub.2CO.sub.3 (0.072 g, 0.523 mmol) were suspended in 600 L of anhydrous DMF. CH.sub.3I (0.111 g, 0.785 mmol) was added in one portion and the mixture heated at 60 C. for 24 hours. The mixture was allowed to cool down to room temperature and then 5 mL of saturated NH.sub.4Cl aqueous solution was added. Compound 16a was isolated by flash column chromatography on silica gel using CH.sub.2Cl.sub.2:MeOH 9:1 for elution. Yield 9.8 mg, 18%. .sup.1H NMR (400 MHz, CDCl.sub.3) 8.29 (dd, J=7.8, 1.1 Hz, 1H), 7.80-7.73 (m, 1H), 7.74-7.67 (m, 1H), 7.60 (t, J=8.0 Hz, 1H), 7.47 (d, J=8.9 Hz, 1H), 7.42 (d, J=7.6 Hz, 1H), 7.35 (dd, J=7.5, 1.0 Hz, 1H), 7.07 (d, J=7.5 Hz, 1H), 6.93 (dd, J=9.1, 5.3 Hz, 2H), 6.68 (q, J=1.8 Hz, 2H), 4.13 (s, 3H), 3.59 (s, 3H). .sup.13C NMR (101 MHz, CDCl.sub.3) 185.38, 165.66, 158.82, 158.40, 151.35, 150.61, 137.97, 135.12, 132.78, 131.21, 130.67, 130.38, 130.32, 129.67, 129.27, 124.52, 123.41, 120.49, 118.58, 116.77, 114.92, 107.91, 105.48, 56.26, 52.39. HR ESI [M+H.sub.+] m/z 411.1231; calc for C.sub.26H.sub.19O.sub.5; 411.1237.
(195) Methylation at R.sup.18 to produce methyl ether 16a dramatically improved the quantum yield. The quantum yield of yellow-orange emitting 16a was 0.4649, 40 times greater than the corresponding neutral compound 15a. It was slightly higher than the yellow-green emitting neutral compound 20a, and >2 times higher than orange-red emitting anion 20c. The compounds (7.5 M) were analyzed in 10:90 DMSO:aqueous solutions. Fluorescence emission was readily visible in a darkened room when the solutions were excited from below with 3-watt megaMAX 505 nm ALS system. The emission-enhancing properties afforded through the combination of the 3-1 transposition and methylation is in contrast to previously reported methyl ethers of seminaphthofluorescein compounds.
(196) The interested reader is referred to online resources at pubs.acs.org/doi/supp1/10.1021/ja302445w/supp1_file/ja302445w_si_001.pdf for additional NMR, ESI, and absorption spectra, as well as HOMO-LUMO surfaces of some embodiments of the disclosed compounds.
Example 2
Emission in Blood
(197) Compound 15d (Table 1) was diluted to 40 M in 50% DMSO:50% 25 mM pH 9 phosphate buffer. As shown in
(198) The close match between compound 15d's absorption and the output of a common laser pointer allowed its excitation with a simple inexpensive light source.
(199) Furthermore, its NIR emission was detected with a commercial-grade digital camera in normal room light combined with an inexpensive Hoya R72 infrared filter (
(200) The absorption of compound 4a (Table 1) was clearly visible and remained stable over extended periods of time in the blood solution. Compound 5a (Table 2) was also found to function in 5% blood. Its absorbance and fluorescence spectra faded over time, but remained visible after about an hour (although with only 10-20% of its original emission intensity). Molecular modeling has shown favorable structural interactions of compounds 5f and 24 with glutathione (
(201) Spectral behavior of compound 15g (type [c] annulated seminaphtho-rhodamine methyl ester (free amine) with the 3-1 transposition) was investigated to determine its potential for sensing use in whole blood. Solutions of compound 15g in 10% or 90% by volume whole blood (porcine blood in Na-EDTA, Lampire Biological Laboratories) were investigated in a 33 mm cell. The fluorophore was first dissolved in 50 mM, pH 7.4 phosphate buffer to ensure complete dissolution, and in the case of 90% whole blood, this stock solution was mixed with whole blood in a 1:9 ratio. In the case of 10% whole blood, 1 part whole blood was added to 9 parts of an appropriately diluted (with deionized water) solution of compound 15g stock. In both cases, the final concentration of compound 15g was 150 M with a final phosphate buffer concentration of 5 mM.
(202) Compound 8 (type [c] fully annulated naphthofluorescein methyl ester with 3-1 transposition) displays a maximum absorption at greater than 800 nm, well past that of hemoglobin absorption in blood. As a result, compound 8 also may perform well in blood.
Example 3
Synthesis and Characterization of Rhodamine Bis-Boronic Acids
(203) Compound 51seminaphthorhodamine bis-boronic acid: With reference to Scheme 9 (
(204) Compound 53naphthorhodamine bis-boronic acid: With reference to Scheme 10 (
(205) Characterization: The spectral properties of compounds 50-53 were determined. Absorption and fluorescence spectra of solutions of the rhodamine bis-boronic acids 51 and 53, and their respective precursors 50 and 52, are shown in
(206) The results are summarized below in Table 5. The seminapthorhodamine 50 maximum absorption in DMSO buffer 1:9 was at 535 nm (40 nm to the red of rhodamine 110). Its maximum emission at 628 nm displayed a reasonable quantum yield of 19% and was red shifted nearly 100 nm as compared to rhodamine 110. The corresponding bis-boronic acid 51 was further red shifted with maximum absorption and emission at 560 and 638 nm, respectively. Emission was slightly quenched through photoinduced electron transfer (PET) in the boron-nitrogen system as evidenced by the lower quantum yield. It is interesting to note that the quenching for the original RhoBo and the fully annulated bis-boronic acid 53 discussed below is much greater than the asymmetric bis-boronic acid 51.
(207) The maximum absorption of napthorhodamine 52 was further shifted to 578 nm with emission wavelength and quantum yield (668 nm, 10%) comparable to commercially available naphthofluorescein. The corresponding bis-boronic acid 53 was further red shifted with maximum absorption and emission at 628 and 692 nm, respectively. Although it displayed a reasonably strong blue/green color, its fluorescence was nearly completely quenched, allowing for a potential turn-on type sensor. Like the other compounds, its emission was slightly redder and its quantum yield was slightly higher in DMSO buffer 1:1. However, like its corresponding rhodamine precursor 52, it exists primarily in the closed and colorless lactone form in this solvent.
(208) TABLE-US-00005 TABLE 5 Abs DMSO:buffer Compound (, M.sup.1cm.sup.1) Ex/Em (Q.Y.) Brightness ratio.sup.a Rhodamine 110.sup.b 495 (66800 @ 492) NA/523 (0.91) 60788 @ 492 NA RhoBo 501 (22149) 502/528 (0.30).sup.c,f 6645 1:9 50 535 (42701) 534/628 (0.19) 8083 1:9 Bis-boronic acid 51 560 (20326) 568/638 (0.15).sup.e 3032.sup.e 1:9 52 578 (11994).sup.d 584/668 (0.10) 1144.sup.d 1:9 Bis-boronic acid 53 628 (10759).sup.d 612/692 (0.004).sup.f 43.sup.d,f 1:9 Rhodamine 110 503 (67905) 502/526 (NA) NA 1:1 RhoBo 504 (21003) 504/528 (NA) NA 1:1 50 542 (21064) 542/630 (0.30) 6298 1:1 Bis-boronic acid 51 579 (23096) 580/643 (0.35) 8037 1:1 52 602 (1081).sup.g 596/676 (0.11) 121.sup.g 1:1 Bis-boronic acid 53 616 (1616).sup.g 624/700 (0.18) 292.sup.g 1:1 .sup.aFinal pH 7.4 phosphate buffer concentration was 12.5 mM. .sup.bSolvent was 10 mM HEPES, pH 7.5, 15% (v/v) EtOH; Values from Leytus et al., Biochem. J. 209, 299 (*1983). .sup.cValue taken from Halo et al., J. Am. Chem. Soc. 131, 438 (2008). .sup.dPartial lactone formation. .sup.eWeak PET quenching, .sup.fStrong PET quenching, .sup.gNear complete lactone formation.
Example 4
Saccharide Sensing and Selectivity
(209) Responses of the boronic acid probes, compounds 51 and 53, were monitored over a wide range of glucose, ribose, and fructose concentrations in various solvents. Solvents including MeCN, MeOH, EtOH, DMSO and buffer were initially screened for sugar sensing. Probes were partially soluble in MeCN and buffer, and soluble in MeOH, EtOH and DMSO. DMSO mixtures were chosen for further studies.
(210) Due to the solvent-dependent lactone ring opening-closing equilibrium (Scheme 11) that these type of probes exhibit, a DMSO titration was carried out, in order to determine the range of possible conditions for sugar sensing. Samples were titrated from 0-60% DMSO. The final pH 7.4 phosphate buffer concentration was 12.5 mM. Absorbance values were measured as follows: seminaphthorhodamine 50532 nm, bis-boronic acid 51578 nm, naphthorhodamine 52578 nm, bis-boronic acid 53627 nm. As shown in
(211) It is interesting to note that the boronic acid derivatives are less prone to lactone formation than their corresponding rhodamine precursors.
(212) ##STR00033##
(213) Initial screening for sensing of glucose, fructose and ribose using these probes in mixtures DMSO:buffer 9:1 (final pH 7.4 phosphate buffer concentration of 12.5 mM) used in our previous work, showed that no changes in either absorbance or fluorescence were observable. Incubation either at room temperature or 37 C. for up to 24 h, gave similar results. It appears that sugar binding did not sufficiently alter the lactone equilibria in this solvent.
(214) The spectral behavior of both compounds 51 and 53 in the presence of saccharides was further investigated in both 1:1 and 1:9 DMSO-buffer (final pH 7.4 phosphate buffer concentration of 12.5 mM) solutions. Both bis-boronic acids responded to binding of sugars through a red shift in their emission and the expected increase in fluorescence intensity.
(215)
(216) There were significant differences between the responses of asymmetric bis-boronic acid 51 and symmetric bis-boronic acid 53.
(217) When the solvent system is mostly aqueous (1:9 DMSO:buffer), solvation by water inhibits intramolecular interactions by salt bridges, allowing the sugar boronate complexes in compound 51 to adopt practically any possible conformation reaching the carboxyl group without problem, especially for the side where the rhodamine system ring is not extended. For the relatively less aqueous system (1:1 DMSO:buffer), the selectivity turns slightly towards ribose due to the enhancement of intramolecular binding interactions between the bound sugar and dye.
(218) Compound 53 has both boronic acid groups far from each other and from the carboxylate compared to compound 51. In the 1:9 DMSO:buffer solvent system the selectivity followed the normal behavior (fructose>ribose>glucose). When the ratio DMSO:buffer is 1:9, the intramolecular electrostatic interactions are practically nonexistent, allowing more freedom to adopt many possible conformations. If saccharide bis-boronates are formed, the interactions between them can bend the planar chromophore slightly to allow interactions with the carboxylate. Upon changing the solvent system to 1:1 DMSO-buffer, the affinity of symmetric compound 53 for ribose was greatly increased, approaching the response observed for fructose at relatively low concentrations. In this more organic solvent system, the asymmetric boronic acid compound 51 reversed its selectivity and responded most strongly to ribose. For the system with DMSO:buffer 1:1 ratio, the electrostatic interactions are very weak, but strong enough to restrict the molecule from adopting many possible conformations.
(219) Both compounds 51 and 53 exhibited selectivity trends (fructose >ribose >glucose) in the 1:9 DMSO:buffer system. Compound 53 is particularly attractive as a candidate for selective sugar determination as it responded exclusively to fructose through a clear to blue-green color change with corresponding turn-on NIR fluorescence enhancement of up to 140-fold and no interference from other sugars at concentrations below 100 mM.
Example 5
Screening Dyes
(220) Embodiments of the disclosed NIR dyes can be screened by determining properties of each fluorophore, including : 1) wavelength of maximum absorption of each form (i.e., neutral and ionized); 2) molar absorptivity of each form; 3) wavelength of maximum excitation of each form; 4) wavelength of maximum emission of each form (corrected for pmt (photomultiplier) response); 5) Stokes shift in units of both nm and energy of each form; 6) complete Excitation-Emission Matrix, EEM (corrected for pmt response) covering a wavelength region that includes solvent adduct, neutral and ionized forms (excitation 335-800 nm; emission 360-850 nm); 7) relative quantum yields of each form; and/or 8) pK.sub.a (or apparent pK.sub.a dependent on the solvent).
(221) A standardized procedure including absorption spectra, excitation emission matrices (EEMs), emission spectra upon common laser-line excitations, and excitation spectra of the observed emission peaks, and/or serial dilutions of a pH titration series will provide the data required to characterize the dyes and their various forms.
(222) The screening can be divided into two steps. The first step would be an initial screen of compounds comprised of:
(223) Step 1-A: A single absorption spectra and EEM of 7.5 M each compound (concentration will be lowered if the absorption is greater than about 0.25, or increased if the absorption is less than about 0.05) in pH 8.25 buffer with 5% DMSO (pH 8.25 is near or slightly greater than the likely pK.sub.a of most of the dyes (with the exception of the proposed fluorinated derivatives), so both anionic and neutral forms will be present in significant (nearly equal) amounts). The DMSO will help with any potential solubility issues of the neutral form (the DMSO amount can be reduced if the dye is sufficiently soluble).
(224) Step 1-B: The same procedure as 1-A, but with 5% buffer, 95% DMSO.
(225) Step 2: Multiple concentrations of the dyes (for molar absorptivity and quantum yield measurements) and multiple pHs (for pK.sub.a measurements) will be investigated for the most promising candidates. When available, appropriate quantum yield standards will allow estimation of absolute quantum yields.
(226) Step 3: Computer-aided molecular modeling can be employed to investigate potential interactions with analytes of interest provided by the various geometries of the analytes and the dye molecules.
(227) Interpretation of results. Steps 1A and 1B will provide a wealth of preliminary data (absorption and emission maxima of each species in both solvents, and an estimate of the relative quantum yield of the long wavelength species in both solvents). This initial first screen will not give any information about molar absorptivity, pK.sub.a, or absolute quantum yields. Selected compounds can be investigated further in Steps 2 and/or 3.
Example 6
Detecting and Quantitating Analytes in Biological Fluids
(228) Promising dyes can be investigated to determine their binding with analytes of interest (e.g., GSH, cysteine, homocysteine, SAICAr, S-Ado) and to determine any potential interferences from blood and/or urine. A known procedure used to quantify binding of albumin dyes to blood components can be utilized (Omoefe et al., J. of Biomed. Optics July 2001, 6(3), 359-365).
(229) Measurements can be made in artificial blood or urine solutions containing the major fluid components and spiked with an analyte of interest. The major blood components and their mean concentration (mg/L) in blood are: HSA41,000, HDL850, LDL810, globulin32,000, red blood cells (43.5% hematocrit) 489,375 (Abugo et al., J. Biomed. Opt., 2001, 6, 359-365; Abugo et al., Anal. Biochem., 2000, 279, 142-150). The chemical composition of human urine has been thoroughly investigated and reported by NASA (NASA Contractor Report CR-1802). The composition of simulated urine and urine solutions will be based on their findings. Five major components (urea, creatinine, oxalate, uric acid and citrate) have previously been used to simulate urine content (Ow et al. IFMBE Proceedings, 2008, Vol. 21, Part 3, Part 11, 742-745; Osman et al., Biomed 2008 Proceedings 2008, 21, 742-745).
(230) Apparent binding constants can be estimated directly from raw titration data without any model assumptions; however, the analytical representation of data may be used to simulate dye behavior in a complex mixture with multiple potential interferences. A phenomenological approach involving fits of experimental titration curves may be used model the experimental data and allow rough estimates of the binding of various dyes in mixtures containing components of biological fluids. It may not be possible to test all blood (or urine) components; however, inclusion of the major components should be sufficient to screen embodiments of the disclosed dyes for selectivity toward analytes of interest. Matrix effects may be evaluated by comparing standard calibration curves in the biological fluids with standard calibration curves in an optimal buffer system.
(231) Selectivity may be fine-tuned based on the experimental results combined with further computer aided molecular simulations. Analysis of GSH and SAICAr and S-Ado may be carried out in authentic biological fluids (GSH in blood and SAICAr and S-Ado in urine) using samples spiked with calibration (reference) standards and using quality control (QC) samples. The developed methodology may be evaluated according to standard procedures for method development including (a) accuracy, (b) precision, (c) selectivity, (d) sensitivity, (e) reproducibility, and (f) stability. Recovery of analyte can be determined using standard addition protocols. All biological fluids to be used may be purchased from commercial sources.
(232) Results from optimal conditions for quantification of the analytes of interest in solution along with a similar methodology as described by Orfanos (Anal. Biochem., 1980, 104, 70-74) may be adapted for the detection and quantitation of the analytes of interest in biological fluids deposited in filter paper. Dried blood (or urine) specimens on filter paper are obtainable. For blood spots, disks may be punched from the paper and the blood contents typically eluted/extracted into a small volume of solvent. In conventional analyses, the absorption from hemoglobin remains a major interference. This is typically overcome by further dilution in order to ensure the hemoglobin content is less than 30 g/ml. Such dilution complicates measurements of analytes present at high concentration and may even prevent analysis of trace analytes. Based on preliminary results (see Example 2), embodiments of the disclosed NIR dyes will function in the presence of at least 5% whole blood. Thus, extraction of the contents of a typical 4.8-mm disk into a small volume (0.6 mL or less, containing one of our dyes) may be used directly without dilution.
(233) In some instances, the dyes may be conjugated to a polymer, e.g., a polyethylene glycol, to provide enhanced selectivity and minimization of interferences. Fluorination of certain embodiments of the disclosed dyes may facilitate solubility in aqueous solutions. In certain instances, dithiothreitol (DTT) may be added to the analysis solution to prevent and/or minimize disulfide formation. At least some embodiments of the disclosed dyes function in the presence of DTT; furthermore, monitoring total GSH-GSSG (via adding DTT to the solution) is feasible since about 90% of blood GSH is in the reduced form and glutathione disulfide (GSSG) levels are similar in both patients and controls (Atkuri et al., PNAS U.S.A., 2009, 106, 3941-3945).
(234) These studies may enable identification of NIR dyes that bind strongly and selectively to analytes of interest. Fluorescence enhancement upon binding may be evaluated and utilized to determine what dye geometries and functional groups favor target analytes or specific interfering components.
Example 7
Glutathione Selectivity Evaluation
(235) Mechanistic studies may be conducted in control experiments (buffered media at physiological conditions or in the presence of a co-solvent if needed) to determine the origin of the selectivity observed for the analytes of interest by embodiments of the disclosed NIR dyes. Conjugates, adducts or reaction products may be isolated and characterized by HPLC and LC-MS ESI. Crystal structures of isolated complexes, adducts or reaction products may be determined by X-Ray crystallography. Binding constants for complex formation between the analytes of interest and NIR fluorescent probes may be obtained using spectrometric methods including: 1H NMR, 2D 1H NMR (ROESY, NOESY), UV-vis or fluorescence.
(236) Selectivity and crossreactivity may be evaluated in the presence of possible interferences (e.g., Cys, Cys-Gly, and/or Cys-Glu for GSH). Influence of factors involved in selectivity/specificity including pH, ionic strength, type of buffering system, and molar ratios of analyte:NIR fluorophore-viologen conjugate may be evaluated using spectrometric methodologies.
Example 8
Selective Detection of SAICAr and S-Ado in Urine
(237) Embodiments of the disclosed NIR dyes functionalized with at least one boronic acid functional group (i.e., NHR.sup.c where R.sup.c is as defined above) may serve as indicators for ADLS deficiency. Mechanistic investigations and characterization studies similar to those performed in Example 5 may be used to determine selectivity for SAICAr and/or S-Ado detection. Promising candidates may be evaluated in urine and/or synthetic urine samples.
(238) Additional patent documents describing subject matter or background information which may be pertinent to the present disclosure include U.S. Publication No. 2008/0261315, U.S. Publication No. 2010/0051826, and WO 2008/011508, each of which is incorporated in its entirety herein by reference.
(239) In view of the many possible embodiments to which the principles of the disclosed invention may be applied, it should be recognized that the illustrated embodiments are only preferred examples of the invention and should not be taken as limiting the scope of the invention. Rather, the scope of the invention is defined by the following claims. We therefore claim as our invention all that comes within the scope and spirit of these claims.