One variation of a system includes a cartridge comprising: a substrate; a sample well integrated into the substrate, defining an upper opening and a lower opening, and configured to receive a test solution comprising a user sample and an amount of a fluorescent probe configured to bind with a target bioparticle to form a target complex; a filter membrane extending across the lower opening and defining a network of pores configured to convey fluid from the sample well and prevent passage of the target complex through the filter membrane. The system further includes a reader comprising: a housing; a cartridge receptacle configured to receive the cartridge; an excitation source configured to illuminate a detection region within the housing; and a detector defining a field of view intersecting the detection region and configured to detect a signal generated by fluid in the sample well and representing presence of the target bioparticle.

The present invention relates to a medical diagnostic device with a cellular biosensor which detects urea and uric acid by means of a synthetic genetic circuit essentially consisting of transcriptional regulator and bio-sensing module.

Provided is a novel fluorescent probe.

A compound of the following general formula (I) or a salt thereof.

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The present invention provides for water-soluble mono- and dicationic fluorescent dyes with the latter exhibiting stable fluorescence at elevated temperatures. The present invention also provides for methods for the production of the fluorescent dyes and for using these dyes in biological assays such as multiplexing qPCR and tissue staining.

An automated microfluidic bioreactor device and methods to rapidly detect drugs of abuse from human sweat are provided. The bioreactor can perform either single-plexed measurements (detecting only one target analyte at a time) or multiplexed measurement (detecting multiple analytes simultaneously). The bioreactor device has a cartridge comprising a capillary array that employs competitive enzyme-linked immunosorbent assay (ELISA) to detect the presence of various drugs or metabolite compounds. For example, four common drugs, methadone, methamphetamine, amphetamine, and tetrahydrocannabinol, were detected rapidly and quantitatively in about 16 minutes with a low sweat sample volume (about 4 μL per analyte) and a large dynamic range (methadone: 0.0016 ng/mL-1 ng/mL; METH: 0.016 ng/mL-25 ng/mL; amphetamine: 0.005 ng/mL-10 ng/mL; THC: 0.02 ng/mL-1000 ng/mL).

The present subject matter is directed to a luminogen exhibiting aggregation induced emission, wherein T1, T2, and T3 comprise one or more polyynes as a conjugated bridge. The present subject matter is also directed to an AIEgen comprising a hydrophilic pyridium group as a strong electron-withdrawing group; a piperazine group as an electron-donating group; and a α-Cyanostilbene; wherein the AIEgen exhibits aggregation induced emission. The present subject matter is directed to a method of synthesizing an AIEgen and is further directed to a method of labeling comprising incubating a subject having cells with a conjugate formed by conjugating an AIEgen with an antibody; and selectively labeling desired cells by turn-on imaging, wherein labeling occurs when the desired cells are selectively stained by fluorescent emission of the AIEgen upon degradation of the antibody after cellular internalization of the conjugate through endocytosis.

Provided is an information processing apparatus (100) including: an image acquiring unit (112) that acquires captured image information of a sample (20) dyed with a fluorescent dye reagent (10), an information acquiring unit (111) that acquires information related to the fluorescent dye reagent (10), a correcting unit (131) that corrects the luminance of the captured image information using a fluorescence fading coefficient that represents the rapidness at which the fluorescence intensity of the fluorescent dye reagent (10) drops, the fluorescence fading coefficient being included in the fluorescent dye reagent (10), and a calculating unit (132) that calculates information corresponding to fluorescent molecules in the captured image information, using the corrected luminance.

The present invention provides the methods of synthesis of [5]helicene compounds and the use of the said compounds conjugating with biomolecules to work as molecular reporter for diagnostic. The compounds in the present invention have the chemical structure illustrated in the formula (1): wherein G is a connecting group composes of 2 carbon atoms selected from the group consisting of ethane and ethylene; A is a separated or connected group selected from the group consisting of cyano and imide; D1 is selected from the group consisting of oxyalkanoic acid, oxyalkanal and oxyalkanesulfonate; and D2 has structure selected from the group consisting of hydroxyl, oxyalkanoic acid, oxyalkanal, alkyl oxyalkanoate, oxyalkanol and oxyalkanesulfonate. The compounds in the present invention compose of aromatic [5]helicene core comprising long it-conjugating system. The said compounds contain functional groups which able to link with biomolecules and they are soluble in water or other solvents that used in binding process with biomolecules. Moreover, owing to having proper chemical structure, the compounds in the present invention exhibit good fluorescent emission in wavelength of 425-675 nm. When the said compounds connected with biomolecules, the biomolecules give good fluorescence and can be detected under ultraviolet radiation. ##STR00001##

Bioluminescent biosensors useful for monitoring and/or quantifying, in vitro or in vivo, activity of the Hippo signaling pathway. The biosensors monitor LATS kinase activity or YAP-TEAD interaction. The biosensors may be used in methods for monitoring and/or quantifying in real-time, in vitro or in vivo, activity of the Hippo signaling pathway, wherein the activity may be LATS kinase activity and/or YAP-TEAD interaction. The biosensors may be provided in kits for monitoring and/or quantifying in real-time, in vitro or in vivo, activity of the Hippo signaling pathway, wherein the activity may be LATS kinase activity and/or YAP-TEAD interaction.

The invention relates to novel fluorescent dyes with multiple negatively charged groups in their ionized form which are 9-aminoacridines or 1-aminopyrene shaving of one of the following general formulae A-E: or salts or protonated forms thereof, wherein the ionizable groups are typically selected from the following: OH, SH, COOH, SO.sub.3H, OSO.sub.3H, SO.sub.2NHCN, P(O)(OH).sub.2, P(O) (OH).sub.2. The invention further relates to the use of these dyes as fluorescent tags, in particular for reducing sugars and glycans, and to carbohydrate-dye conjugates comprising these dyes as well as to methods for preparing the same.

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