Use of a cysteine protease of plasmodium vivax

Abstract

A use of vivapain-4 (VX-4), which is a cysteine protease of Plasmodium vivax, showing pH-dependent switching of substrate specificity, is provided. More specifically, a method of treating a parasitic disease caused by Plasmodium vivax by inhibiting VX-4; a method of screening a protease inhibitor acting on VX-4, wherein the protease inhibitor is useful as an anti-malarial agent acting on Plasmodium species, for example, Plasmodium vivax; and a method of identifying the activity of VX-4, are provided.

Claims

1. A cysteine protease, wherein the amino acid sequence of the cysteine protease is the amino acid sequence from SEQ ID NO: 1, and wherein glutamic acid at 180th position of SEQ ID NO: 2, which is a mature domain of SEQ ID NO: 1, is deleted, or substituted with an amino acid selected from the group consisting of arginine, histidine, lysine, alanine, isoleucine, leucine, methionine, phenylalanine, proline, tryptophane, valine, and cysteine.

2. The cysteine protease of claim 1, wherein the cysteine protease loses a degrading activity against a dipeptidyl substrate favored by cathepsin B at pH 6.6 to 9, wherein the dipeptidyl substrate has at least one hydrophilic amino acid.

3. The cysteine protease of claim 2, wherein the hydrophilic amino acid is selected from aspartic acid, glutamic acid, arginine, histidine, and lysine.

4. The cysteine protease of claim 2, wherein the dipeptidyl substrate is benzyloxycarbonyl-L-arginyl-L-arginine 4-methyl-coumaryl-7-amide.

5. A method of altering a substrate specificity of a cysteine protease according to claim 1, comprising deleting glutamic acid at 180th position of SEQ ID NO: 2, or substituting glutamic acid at 180th position of a mature domain of SEQ ID NO: 2 with an amino acid selected from the group consisting of arginine, histidine, lysine, alanine, isoleucine, leucine, methionine, phenylalanine, proline, tryptophane, valine, and cysteine, wherein the SEQ ID NO: 2 is a mature domain of SEQ ID NO: 1.

6. The method of claim 5, wherein a degrading activity against a dipeptidyl substrate is eliminated, wherein the dipeptidyl substrate is favored by cathepsin B at pH 6.6 to 9, and has at least one hydrophilic amino acid.

7. The method of claim 6, wherein the hydrophilic amino acid is selected from aspartic acid, glutamic acid, arginine, histidine, and lysine.

8. The method of claim 6, wherein the dipeptidyl substrate is benzyloxycarbonyl-L-arginyl-L-arginine 4-methyl-coumaryl-7-amide.

Description

BRIEF DESCRIPTION OF DRAWINGS

(1) FIGS. 1A and 1B show a multiple alignment of amino acid sequence of vivapain-4 and its homologs in Plamodium genomes.

(2) FIG. 2 shows results of phylogenetic analysis and amino acid substations of vavapain-4.

(3) FIG. 3 shows biochemical properties of recombinant vivapain-4.

(4) FIG. 4 shows results of modeling and mutation analyses.

(5) FIG. 5 shows reactivity of vivapain-4 against macromolecular substrates.

(6) FIG. 6 shows spatiotemporal localization of vivapain-4 by immunocytochemical staining.

EXAMPLE

(7) All animals used in this study were housed in accordance with guidelines from the Association for the Assessment and Accreditation of Laboratory Animal Care (AAALAC). All protocols were approved by the Institutional Review Board and conducted in the Laboratory Animal Research Center of Sungkyunkwan University.

Example 1

In Silico Identification of Cysteine Protease Gene

(8) 1.1: Sequencing of Novel Cysteine Proteases

(9) Genes putatively coding for cysteine proteases were identified from primate and rodent Plasmodium sequences deposited in PlasmoDB (http://plasmodb.org), TIGR (http://www.tigr.org), and GenBank (http://www.ncbi.nlm.nih.gov/) through BLAST searches. The amino acid (AA) sequences of cysteine proteases of P. falciparum (FP-2 [XP_001347836](SEQ ID NO: 19), FP-2B [XP_001347832](SEQ ID NO: 20), and FP-3 [XP_001347833](SEQ ID NO: 18)), P. vivax (VX-2 [XP_001615274] (SEQ ID NO: 14) and VX-3 [XP_001615273](SEQ ID NO: 16)), P. yoelii (yoelipain-2 [YP-2; XP_726900](SEQ ID NO: 21)), and P. berghei (bergheipain-2 [BP]-2 [XP_680416](SEQ ID NO: 22)), P. chabaudi (chabaupain-2 [CP-2], AAP43630(SEQ ID NO: 23)), P. vinckei (vinckepain-2 [VP-2], AAL48319(SEQ ID NO: 24)), P. knowlesi (knowlepain-2 [KP-2], CAQ39926(SEQ ID NO: 15); KP-3, CAQ39925(SEQ ID NO: 17); and KP-4, CAQ39924(SEQ ID NO: 13)), P. falciparum (FP-2, XP_001347836(SEQ ID NO: 19); FP-2B, XP_001347832(SEQ ID NO: 20); and FP-3, XP_001347833(SEQ ID NO: 18)), and P. vivax (VX-2 [XP_001615274] (SEQ ID NO: 14) and VX-3 [XP_001615273](SEQ ID NO: 16)) were used in multiple queries, with a threshold at 0.001 (E-value cut-off). After excluding redundancies, the AA sequences were aligned with ClustalX and optimized with GeneDoc. The alignment was used as an input in the construction of neighbor joining and maximum likelihood trees using PHYLIP (ver. 3.6b) and TREE_PUZZLE (ver. 5.2). The standard error in each of the connecting nodes was estimated by bootstrapping of 1000 replicates.

(10) Two novel cysteine proteases isolated from P. vivax were annotated as P. vivax cysteine protease 1 (VX-1; XP_001615807) and 4 (VX-4; XP_001615272), according to their clustering patterns in the trees.

(11) The obtained results revealed that the P. vivax genome encodes four closely related vivapains. By data-mining of the P. vivax genome (TIGR, Release 2.0), two genes putatively coding for novel cysteine proteases were identified, in addition to the previously identified genes encoding VX-2 (PlasmoDB code PVX_091415) and VX-3 (PVX_091410). The inventors designated these genes as VX-1 (PVX_195290) and VX-4 (PVX_091405). The other primate Plasmodium genomes examined, such as P. falciparum, P. reichenowi and P. knowlesi, also harbored four closely related cysteine protease genes. Conversely, avian and rodent malaria parasites including P. gallinaceum, P. yoelii, and P. berghei possessed only two paralogous genes (FIGS. 1A and 1B).

(12) FIGS. 1A and 1B show a multiple alignment of amino acid sequence of vivapain-4 and its homologs in Plamodium genomes. Numbers of amino acids (AAs) in full-length polypeptides are marked at right side of each of the alignments and numerical in parentheses indicates those of mature forms. Dots indicate gaps introduced into the alignment to maximize similarity values. Boxes indicate sequence motifs of interest based on the FP-2 structure. The ERFNIN and GNFD signatures of prodomains are marked by red letters. Shading marks a putative starting position of each mature domain (SEQ ID NO: 2). Red arrow in inhibitor 129 domain box (FIG. 1A) indicates amino acid position corresponding to the N-terminal region of recombinant VX-4. Three AA residues of S2 pocket, which were selected for the mutagenesis experiments, are indicated by dotted red circles (FIG. 1B). In the figures, YP-2 refers to yoelipain-2 (XP_726900); BP-2, berghepain-2 (XP_680416); CP-2, chabaupain-2 (AAP43630); VP-2, vinckepain-2 (AAL48319); FP-2, falcipain-2 (XP_001347836); FP-2B, falcipain-2B (XP_001347832); FP-3, falcipain-3 (XP_001347833); KP-4, knowlepain-4 (CAQ39924); VP-4, vivapain-4 (XP_001615272); KP-2, knowlepain-2 (CAQ39926); VP-2, vivapain-2 (XP_001615274); KP-3, knowlepain-3 (CAQ39925); VP-3, vivapain-3 (XP_001615273).

(13) The deduced AA sequence of VX-4 (TC5625, 484 AAs) revealed considerable degrees of identity to that of VX-2 (TC5622, 59%) and VX-3 (TC5618, 48%), while that of VX-1 (TC5613, 583 AAs) was highly related to the FP-1-like proteases of P. falciparum, P. knowlesi, P. ovale and P. fragile (37-77% identity). The greater length of VX-1 might be attributable to an N-terminal extension [Na B K, Kim T S, Rosenthal P J, Kong Y (2004) Evaluation of cysteine proteases of Plasmodium vivax as antimalarial drug targets: Sequence analysis and sensitivity to cysteine protease inhibitors. Parasitol Res 94:312-317].

(14) Physiological implications and specific domain(s)/signature(s) of VX-1 remain largely elusive. The primary structure of VX-4 tightly conserved the AA residues lining the catalytic site (Gln, Cys, His, Asn and Trp) that are essential for the stabilization of a thiolate-imidazolium ion pair and/or the transition state of the catalytic site (AA positions highlighted in blue in FIG. 1B). As shown in FIGS. 1 and 1B, the regulatory motifs of the plasmodial cysteine proteases such as a bipartite trafficking domain, inhibitor domain with ERFNIN signature and hemoglobin-binding FP2 arm were also clearly identified in each of the corresponding regions.

(15) The eight Cys residues, which are involved in the maintenance of structural geometry, were well conserved in these proteins, whereas the last Cys was replaced by Asn in VX-4 and KP-4 (arrowheads in FIG. 1B). Given the fact that a disulfide bridge between the seventh and eighth Cys residues is intimately engaged in the stabilization of the S2 and S1 sites of FP-2, the more flexible binding pocket of VX-4 might allow broader accessibility of proteolytic substrates. In addition, several AA substitutions found in critical domains of VX-4 suggest a distinctive physiological role for this protease (FIGS. 1A and 1B). These collective data demonstrate that VX-4 is a distinct cysteine protease that shares significant identity with, but clearly differs from previously characterized P. vivax cysteine proteases.

(16) 1.2: Phylogenetic Analysis

(17) This experiment suggests that Plasmodium cysteine proteases exhibit differential evolutionary episodes along with their donor organisms.

(18) A neighbor-joining tree of VX-1 and VX-4 homologs, which were retrieved from PlasmoDB and GenBank, was constructed employing the AA sequences of mature domains (FIGS. 2). The Plasmodium proteases were largely separated into two distinct clusters consistent with their predicted biological roles: FP-1 clade, of which members are implicated in host cell invasion and oocyst production, and FP-2clade, the majority of which play central roles in hemoglobin degradation.

(19) An overall topology similar to that of the neighbor-joining tree was observed in a quartet maximum likelihood tree (TREE_PUZZLE program; ver. 5.2) and the major branching nodes were supported by significant bootstrapping or quartet values. The falcipain homolog genes appeared to have duplicated from a common ancestor before diverging into each of the avian and mammalian parasite lineages. The FP-1-family proteins seemed to have diverged along with their specific donor organisms without any provocative genetic event. Meanwhile, members of FP-2 clade might have more complicated evolutionary pathways, including either multiplication(s) in primate malaria or deletion(s) in rodent malaria. The genes orthologous to VX-2 and VX-3 may have been deleted in the rodent parasites, considering the polytomic relationships among the P. vivax and P. knowlesi paralogs and the tight clustering of VX-4/KP-4 with rodent malarial proteins. This suggestion is further supported by the fact that P. falciparum and P. reichenowi, which comprise a basal clade in mammalian Plasmodium lineages, contain three paralogous genes. The three paralogous genes occupying distinct but highly linked genomic loci (cysteine protease island) may have undergone a kind of convergent evolution events in these basal malaria genomes.

(20) FIG. 2 shows results of phylogenetic analysis of malaria cysteine proteases including VX-4. The phylogeny was based on the AA sequence alignment of mature regions. Divergence rates were calculated with the Jones-Taylor-Thornton (JTT) substitution model (see Jones D T, Taylor W R & Thornton J M (1992) The rapid generation of mutation data matrices from protein sequences. Computer Applications in the Biosciences 8: 275-282), and the tree was constructed using the neighbor joining algorithm (see Saitou N, Nei M (1987) The neighbor-joining methoda new method for reconstructing phylogenetic trees. Molecular and Biology and Evolution 4:406-425). The tree was rooted with GP-1 of Plasmodium gallinaceum, which was taken as an out-group. The number at each of the branching nodes indicates the likelihood (percentage) of its appearance in the bootstrapping analysis with 1000 replicates. The enzymes from P. vivax are in bold. The box indicates AAs found in the S2 pocket of primate plasmodial proteases, with position numbers based on mature VX-4. Red, blue, and black AAs are acidic, uncharged polar and hydrophobic, respectively. Note: The vivax protein with accession no. XP_001615272 was annotated as VX-2 during primary analysis of the whole genome sequence of the P. vivax Sal I strain. The name is changed to VX-4 according to our current result (AAT91956).

(21) Adding to increased genic dosage, the degree of sequence divergence was prominent among the primate FP-2-clade members (0.8120.078), compared to related rodent proteins (0.2710.034). The members of primate (0.2660.035) and rodent (0.3770.056) FP-1 clade displayed values similar to that of the rodent FP-2-like proteins (Table 1).

(22) TABLE-US-00001 TABLE 1 Pairwise divergence matrix of plasmodial falcipain homologs (FPs) based on the Jones-Taylor-Thornton model.sup.a Group.sup.b 1 2 3 4 1, Rodent FP-1 0.377 0.056 1.261 0.130 3.032 0.480 2.735 0.393 2. Primate FP-1 0.266 0.035 2.840 0.366 2.466 0.338 3. Rodent FP-2 0.271 0.034 0.917 0.0112 4. Primate FP-2.sup.c 0.812 0.078 .sup.aDistance values are presented as mean standard error. The standard error was computed by bootstrapping of 1,000 replicates after removing gaps as missing information in a pairwise manner. .sup.bThe malaria cysteine proteases were categorized into each of the groups based on a phylogenetic analysis (see FIG. 2). .sup.cThe highly redundant cysteine proteases of P. falciparum (FP-2B) and P. reichenowi (RP-3) were excluded in the analysis.

(23) Alteration in gene copy number provides a simple way to change expression levels or to enlarge protein pools with non-overlapping functions. Biochemical studies have demonstrated that the primate malaria proteins belonging to the FP-2 clade exhibit similar enzymatic properties; however, those of P. vinckei (VP-2) and P. berghei (BP-2) demonstrated quite dissimilar features, particularly in terms of their substrate preference and inhibitor specificity. Therefore, the large divergence among the primate FP-2 proteins and tight clustering of VX-4 and KP-4 with rodent Plasmodium proteins (bootstrapping value 76) further suggest biological roles of VX-4 that are distinct from those previously described for VX-2 and VX-3.

Example 2

Expression and Refolding of a Recombinant VX-4 (rVX-4)

(24) The open reading frame (ORF) of VX-4 was amplified with forward (5-ATGGAATATCACATGGAGTACTCGAAC-3, SEQ ID NO: 3) and reverse (5-CTAGTCAAGCAGGGGGACGTACGCCTC-3, SEQ ID NO: 4) primers using Ex Taq DNA polymerase (Takara, Japan) and P. vivax genomic DNA (100 ng) isolated from a Korean patient (a generous gift from Dr. J S Yeom [Sungkyunkwan University Hospital]). The PCR conditions were as follows:

(25) 94 C. 5 min

(26) 94 C. 1 min, 50 C. 1 min, 72 C. 2 min: 30 cycles

(27) 72 C. 10 min

(28) The product was gel-purified, ligated into the pCR2.1 vector (Invitrogen, CA) nd transformed into competent E. coli Top10 cells (Invitrogen) by heat shock (ice 30 minutes, 42 C. 45 seconds, ice 5 minutes). The purification, ligation and transformation were conducted according to a manual provided by Invitrogen. The nucleotide sequence was determined with an ABI PRISM 377 DNA sequencer (Applied Biosystems, CA).

(29) The obtained DNA fragment harboring the mature region and a portion of the prodomain from AA position 182 of SEQ ID NO: 1 were amplified using Ex Taq DNA polymerase (Takara, Japan) and 2 primers; 5-GAGCTCGAGATGCAGCAGAGGTACCT-3 (SEQ ID NO: 5, containing a 5 Sac I site) and 5-CTGCAGCTAATCCACGAGCGCAACGA-3 (SEQ ID NO: 6, containing a 5 Pst I site).

(30) The PCR conditions were as follows:

(31) 94 C. 5 min

(32) 94 C. 1 min, 50 C. 1 min, 72 C. 2 min: 30 cycles

(33) 72 C. 10 min

(34) The PCR product was ligated and transformed as described above, and ligated into the pQE-30 expression vector (Qiagen, CA) using Sad and PstI. The plasmid was transformed into competent E. coli M15 (pREP4) cells (Qiagen) by heat shock (ice 30 minutes, 42 C. 45 seconds, ice 5 minutes), grown overnight in LB broth (containing 100 ug/ml ampicillin and 50 ug/ml kanamycine) and induced with 1 mM isopropyl-1-thio--D-galactopyranoside for 3 h at 37 C. The bacterial cells were suspended in lysis buffer (8 M urea, 10 mM Tris-HCl, 100 mM NaH2PO4, pH 8.0) and then centrifuged at 12,000 g for 20 minutes to collect supernatant.

(35) rVX-4 was purified from the supernatant by nickel-nitrilotriacetic acid (Ni-NTA agarose, Qiagen) chromatography, following the manufacturer's instruction. Optimal refolding conditions for rVX-4 were determined with 100 different buffer combinations in a microplate format [Sijwali P S, Brinen L S, Rosenthal P J (2001) Systematic optimization of expression and refolding of the Plasmodium falciparum cysteine protease falcipain-2. Protein Expr Purif 22:128-134].

(36) The expressed and purified rVX-4 protein comprising a portion of the prodomain and entire mature domain was subject to 12% SDS-PAGE analysis with coomassie blue staining, and the obtained results are shown in FIG. 3(A) (Lanes a, uninduced E. coli lysate; b, IPTG-induced E. coli lysate; c, Ni-NTA purified rVX-4. M.sub.r, molecular masses in kDa).

(37) For large-scale refolding, purified rVX-4 (100 mg) was diluted 100-fold in optimized refolding buffer (250 mM L-arginine, 1 mM ethylenediaminetetraacetic acid [EDTA], 5 mM reduced glutathione [GSH], 1 mM oxidized glutathione [GSSG], and 100 mM Tris-HCl, pH 8.0), and incubated overnight at 4 C. To allow processing to the active enzyme, the pH was adjusted to 5.5 in the presence of 10 mM dithiothreitol (DTT), the sample was incubated at 37 C. for 2 h, and the pH was then readjusted to 6.5. The protein was concentrated with a Centriprep concentrator (cut-off: 10 kDa, Millipore).

(38) The rVX-4, which was refolded followed by maturation under reducing and mild acidic (pH 5.5) conditions, was subject to 12% SDS-PAGE analysis with coomassie blue staining, and the obtained results are shown in FIG. 3(B). FIG. 3(B) shows processing of the refolded rVX-4. The purified rVX-4 was refolded and activated, and aliquots collected every 30 min were analyzed by 12% SDS-PAGE with Coomassie staining (left). The proteolytic activity of fully processed rVX-4 was analyzed by a gelatin gel-zymogram (right). The fully processed 28 kDa protein (left panel, FIG. 2(B)) exhibited protease activity by gelatin-gel electrophoresis (right panel, FIG. 2(B)), which was completely inhibited by the cysteine protease inhibitor E-64.

Example 3

N-Terminal Amino Acid Sequencing

(39) The fully processed rVX-4 was separated by 12% SDS-PAGE. The protein was transferred to a polyvinylidene difluoride (PVDF) membrane (Millipore) and stained with Coomassie blue. The band was excised and subjected to protein sequencing on an ABI model 477A protein sequencer and an ABI model 120A PTH analyzer (Applied Biosystems) at the Korea Basic Science Institute (Daejeon, Korea). The obtained N-terminal amino acid sequence is shown in red box of FIG. 1B, from which mature rVX-4 is initiated.

Example 4

Specific Antibodies

(40) Six-week-old, specific pathogen free (SPF) BALB/c female mice were subcutaneously immunized 3 times with the purified rVX-4 (30 g per each mouse per each time) in Freund's adjuvants (Sigma-Aldrich) at 2-week intervals. One week after the final inoculation, 10 g protein were injected via tail vein. One week later, the blood was collected by heart puncture, after which the antiserum was prepared. BALB/c mouse (6-week-old) serum obtained from SPF strain was used as a normal control.

Example 5

Cysteine Protease Activity Assay and Kinetics

(41) Cysteine protease activity was ascertained by the hydrolysis of benzyloxycarbonyl-L-leucyl-L-arginine 4-methyl-coumaryl-7-amide (Z-LR-MCA) (Peptide International, Louisville, Ky.). Enzyme (xVX, 30 l; 200 nM) was added to 100 mM sodium acetate (220 l, pH 5.5) containing 5 M Z-LR-MCA and 10 mM DTT. The release of fluorescence was assessed at excitation and emission wavelengths of 355 nm and 460 nm with a SpectraMAX Gemini fluorometer (Molecular Devices, Sunnyvale, Calif.).

(42) For activity gel electrophoresis, the obtained refolded rVX-4 was mixed with SDS-PAGE sample buffer lacking 2-mercaptoethanol and subjected to 12% SDS-PAGE co-polymerized with 0.1% gelatin. The gel was washed with 2% Triton X-100 (30 min), incubated overnight with 100 mM sodium acetate (pH 5.5) containing 10 mM DTT at 37 C. and stained with Coomassie Blue.

(43) For kinetic analysis, the rVX-4 (25 nM) was incubated with varying concentrations of peptide substrates (Z-LR-MCA) at pH 5.5, 6.5 and 7.5 in appropriate buffers (100 mM sodium acetate (pH 4.5-5.5), 100 mM sodium phosphate (pH 6.0-6.5) and 100 mM Tris-HCl (pH 7.0-8.5)), each supplemented with 10 mM DTT. The release of MCA was monitored over 10 min at room temperature as described above. Activities were compared as fluorescence over time. The kinetic constants K.sub.m and V.sub.max were determined using GraphPad software.

(44) The optimal pH was assessed in 100 mM sodium acetate (pH 4.5-5.5), 100 mM sodium phosphate (pH 6.0-6.5) and 100 mM Tris-HCl (pH 7.0-8.5). The enzymes (50 nM) were added to each buffer supplemented with 10 mM DTT and 5 M Z-L-phenylalanyl-L-arginine 4-methyl-coumaryl-7-amide (Z-FR-MCA), Z-leucyl-L-arginine-MCA (Z-LR-MCA), or Z-L-arginyl-L-arginine 4-methyl-coumaryl-7-amide (Z-RR-MCA) (Peptide International). The appropriate buffers were separately employed as controls at each pH. Enzyme activity was measured as described above. The effects of reducing agents were examined under various concentrations of GSH, and pH stability was examined at pH 5.0 and 8.0 by incubating rVX-4 at 37 C. in the appropriate buffer. Active site titration was done using a specific inhibitor, trans-epoxysuccinyl-L-leuciloamido-(4-guanidino) butane (E-64).

(45) rVX-4 hydrolyzed synthetic dipeptidyl substrates with hydrophobic AA residues at their P2 site such as Z-LR-MCA and Z-FR-MCA under acidic conditions as described above. The determined enzyme activity, stability, and inhibition of The VX-4 are shown in FIGS. 3(C), 3(D), and 3(E), respectively.

(46) FIG. 3(C) relates to determination of pH optimum. The VX-4 enzyme activity was assayed in 100 mM sodium acetate (pH 4.5-5.5), sodium phosphate (pH 6.0-6.5) or Tris/HCl (pH 7.0-8.5), each supplemented with 10 mM DTT. Activity was measured at 37 C. each against Z-FR-MCA (.square-solid.), Z-LR-MCA (), and Z-RR-MCA (.circle-solid.). Maximal activity was presented as 100%. As shown in FIG. 3(C), the enzymatic activity was highest at pH 5.5. The pH-optimum was substantially different with a substrate containing a basic AA at P2 (Z-RR-MCA) with maximal activity at pH 6.5, and activity seen above pH 8. These results suggest either that electrostatic conditions near the S2 site are highly dependent on the surrounding pH or that the geometry of the catalytic site can be changed in a pH-dependent manner.

(47) FIG. 3(D) relates to determination of enzyme stability depending on pH. rVX-4 was incubated at different pHs in the respective buffers as in panel C. Residual activity was assayed with Z-LR-MCA in 100 mM sodium acetate (pH 5.5) supplemented with 1 mM DTT after indicated incubations at pH 4.5 (.circle-solid.), 5.0 (), 5.5 (.box-tangle-solidup.), 6.0 (), 7.0 (), 8.0 () and 8.5 (.square-solid.). rVX-4 was relatively stable after incubation at acidic and neutral pH, while it was highly unstable under alkaline conditions (pH 8.0 and 8.5) against Z-LR-MCA, which was similar to results observed for FP-2 and FP-3. These data suggest that decreased hydrolyzing activity of rVX-4 against Z-LR-MCA and Z-FR-MCA at alkaline conditions might be due to an irreversible change of the protein conformation.

(48) FIG. 3(E) shows a inhibition profile for E-64, which was determined by incubating rVX-4 (1 M) with different concentrations of E-64 in 100 mM sodium acetate (pH 5.5; .circle-solid.), 100 mM sodium phosphate (pH 6.5; ) or 100 mM Tris-HCl (pH 7.5; .square-solid.) at room temperature for 30 min. Residual activities (%) were determined using Z-LR-MCA as a substrate. The requirement for increased concentrations of E-64 to inhibit rVX-4 at higher pH also supports altered structural geometry as the explanation for altered substrate preference.

(49) Steady-state kinetic analyses confirmed varied substrate utilization depending on pH, and the obtained results are shown in Table 1.

(50) TABLE-US-00002 TABLE 1 Comparison of substrate hydrolysis kinetics for vivapains k.sub.cat/K.sub.m (s.sup.1M.sup.1) VX-2 VX-3 VX-4 pH 5.5 Z-FR-MCA NH.sup.a NH 1.55 10.sup.4 Z-LR-MCA 7.05 10.sup.5 8.62 10.sup.4 1.65 10.sup.4 Z-RR-MCA NH NH 1.34 10.sup.4 pH 6.5 Z-FR-MCA NH NH 5.52 10.sup.3 Z-LR-MCA 7.34 10.sup.5 6.36 10.sup.4 8.84 10.sup.3 Z-RR-MCA NH NH 3.49 10.sup.4 pH 7.5 Z-FR-MCA NH NH NH Z-LR-MCA 4.15 10.sup.5 6.26 10.sup.3 3.05 10.sup.3 Z-RR-MCA NH NH 3.45 10.sup.4 Activity values for each enzyme represent mean from three independent experiments. .sup.aNH, no hydrolysis.

(51) As shown in Table 1, rVX-4 showed a similar catalytic efficiency against three peptide substrates at pH 5.5. However, at pH 7.5 k.sub.cat/K.sub.m against Z-RR-MCA increased 2.6-fold whereas that against Z-LR-MCA decreased 5.6-fold and Z-FR-MCA was not hydrolyzed. The rVX-2 and rVX-3 proteins exhibited much higher k.sub.act/K.sub.m values than that of rVX-4 toward Z-LR-MCA at the pH conditions selected, although the optimal pH for rVX-2 was 6.5, rather than 5.5. Interestingly, rVX-2 and rVX-3 could not hydrolyze Z-FR-MCA or Z-RR-MCA. Phe has a large aromatic R group, and it might not fit into the S2 pocket of rVX-2 and rVX-3, which are stabilized by the disulfide bond between the seventh and eighth Cys residues (FIGS. 1A and 1B).

Example 7

Hydrolysis of Macromolecular Substrates

(52) To observe possible roles of VX-4 in the processing of plasmepsin (PM), the inventors cloned P. vivax plasmepsin (PvPM) 4 (XM_001616821) and 5 (XM_001615583) employing P. vivax genomic DNA obtained from the Korean patient as previously described [Dame J B, Yowell C A, Omara-Opyene L, Carlton J M, Cooper R A, Li T (2003) Plasmepsin 4, the food vacuole aspartic proteinase found in all Plasmodium spp. infecting man. Mol Biochem Parasitol 130:1-12].

(53) Recombinant PvPMs expressed in E. coli cells were purified by Ni-NTA chromatography (Qiagen) and refolded as described above. rVX-4 (50 nM) was incubated with PvPMs (20 g each) in 100 mM sodium acetate (pH 5.0-5.5), 100 mM sodium phosphate (pH 6.0-6.5), or 100 mM Tris-HCl (pH 7.0-7.5) supplemented with 10 mM DTT for 3 h. The experiments were also performed in the presence of E-64 (1 M) and/or pepstatin A (10 M, Sigma-Aldrich). Hemoglobinase activity of rVX-4 (30 nM), as well as those of rVX-2 and rVX-3 expressed as previously described [Na B K, Shenai B R, Sijwali P S, Choe Y, Pandey K C, Singh A, Craik C S, Rosenthal P J (2004) Identification and biochemical characterization of vivapains, cysteine proteases of the malaria parasite Plasmodium vivax. Biochem J 378:529-538], was assessed using human hemoglobin (Sigma-Aldrich) in different pHs (5.0-7.5) in the presence of 1 mM GSH at 37 C.

(54) Erythrocyte ghosts purified from fresh human blood by hypotonic lysis were incubated with rVX-4 (200 nM) at pH 7.0 or 7.5 at 37 C. for 3 h, after which reaction products were analyzed by reducing SDS-PAGE. For immunoblotting, the electrophoretically resolved proteins (rVX-4) were transferred to PVDF membranes (Millipore) followed by blocking with 0.05% Tween 20 in phosphate buffered saline (PBST) containing 2% bovine serum albumin. The membrane was incubated with appropriate antibodies including anti-human spectrin (Sigma-Aldrich, 1:500 dilutions), anti-human band 3 (Sigma-Aldrich, 1:3000 dilutions), or anti-human actin (Sigma-Aldrich, 1:1000 dilutions). Blots were subsequently incubated with horseradish peroxidase-conjugated host specific antibodies (Cappell). The immunoreactive bands were visualized using 4-chloro-1-naphthol (4C1N; Sigma-Aldrich) supplemented with 3% hydrogen peroxide.

(55) The obtained results suggest that VX-4 may exert its activity in maturation of plasmepsin and digestion of erythrocytic actin, while having adjuvant roles in hemoglobin hydrolysis. Comparative analysis revealed that two motifs, the FP2 nose and FP2 arm, specific to the hemoglobin-degrading falcipain homologs, were conserved in VX-4 (FIGS. 1A and 1B). The FP2 nose interacts with the protease core via a highly conserved KEA motif to provide proper folding of the mature protein, while the FP2 arm mediates interaction between the enzyme and hemoglobin. The inventors recognized some differences in the FP2 arm motif of VX-4, in which residues Phe192, Ser194 and Ala198 (numbered from the mature sequence of VX-4) showed different degrees of hydropathy compared to those of other VXs. In addition, Ala198 of VX-4 offered a unique hydrophobic polymorphism, which in structural modeling contributed considerable change in the arm structure. These observations suggested that VX-4 may act principally on substrates other than hemoglobin.

(56) It was assessed whether VX-4 plays a role in plasmepsin processing since a recent study has revealed that FPs function as maturases for plasmepsins within the food vacuole of P. falciparum. Plasmodium species infecting mammals harbored genes for seven PMs (PM4-PM10), of which PM4 orthologs were found in the food vacuole. P. falciparum genome encoded additional food vacuole-related proteins, PM1, PM2, and histo-aspartic protease (HAP), although genes orthologous to these proteins genes were not detected in non-falciparum species. These results suggest that PvPM4 is the major, if not all, plasmepsin targeted into the food vacuole of P. vivax.

(57) The inventors examined possible roles for VX-4 during maturation of recombinant PvPM4 (rPvPM4), which was expressed in E. coli as above, and the results are shown in FIG. 5(A). FIG. 5(A) relates to processing of P. vivax plasmepsin 4 (PvPM4) by rVX-4. Recombinant PvPM 4 (20 g) was incubated with rVX-4 (50 nM) supplemented with 10 mM DTT at different pH values with or without pepstatin A (PepA, 10 M) or E-64 (1 M) for 3 h at 37 C. The reactants were analyzed by 12% SDS-PAGE. As shown in FIG. 5(A), autocatalytic processing of rPvPM4 occurred at acidic pH and, to a less extent, at neutral pH (6.5-7.0). This processing was completely blocked by the aspartic protease inhibitor pepstatin A. This cleavage was significantly accelerated in the presence of VX-4 in a dose- and time-dependent manner. In the presence of pepstatin A to block autocatalysis, rVX-4 effectively cleaved rPvPM4 at pH 5.0-7.0, and this process was specifically and significantly inhibited by E-64. These results suggest that VX-4 is a key molecule regulating PvPM4 maturation. Processing may occur during trafficking of the enzymes from endoplasmic reticulum (ER)-derived transport vesicles or the parasitophorous vacuolar space (PVS), where pH is neutral, or in the acidic food vacuole (pH 5.4-5.5). VX-2/VX-3 might also participate in the processing in the food vacuole.

(58) The major hemoglobinases of P. falciparum are targeted into the food vacuole through ER-derived vesicles, but it is unclear whether the ER-derived, protease-containing vesicles fuse with hemoglobin-containing transport vesicles derived from cytosomes, or if they directly contact the food vacuole. The bipartite signals, composed of cytoplasmic, transmembrane and lumenal motifs, were found to be required for trafficking of FP-2 and FP-3 to the food vacuole, and they are conserved in VX-2, VX-3, and VX-4 (FIGS. 1A and 1B). The hemoglobinase activity of VX-4 was compared to that of VX-2 and VX-3. pH-dependent and time-lapse analyses demonstrated that the hemoglobinolytic activity of VX-4 was relatively weak. Maximal hemoglobin degrading activity of VX-2, VX-3 and VX-4 was observed between pH 6.0-6.5, 5.0-6.0, and 5.5-6.0, respectively (FIG. 5(B)). FIG. 5(B) shows the comparison of hemoglobinolytic activity of VX-2, VX-3 and VX-4. Native human hemoglobin was incubated with the respective enzymes in appropriate buffers (pH ranges 5.0-7.5) supplemented with 1 mM GSH for 3 h at 37 C., after which resolved by 10% SDS-PAGE. Considering their peak activities at different pHs, the action points of different VXs may be temporally segregated during hemoglobin degradation. However, it is unclear whether the biochemical differences between VX-2, VX-3, and VX-4 are most important to foster cooperative action against hemoglobin or to provide activities against different substrates over the course of erythrocytic infection by P. vivax.

(59) To consider other potential substrates for VX-4, hydrolytic activity against erythrocyte cytoskeletal proteins was examined, and the results are shown in FIGS. 5(C) and 5(D). FIG. 5(C) shows the results of hydrolysis of erythrocyte membrane proteins by rVX-4 at different pHs. Fresh erythrocyte ghosts were incubated with rVX-4 in appropriate buffers (pHs 5.0-7.5) for 3 h at 37 C. and reaction products were analyzed by 10% SDS-PAGE. Molecular masses in kDa are shown to the right. FIG. 5(D) shows the results of western blotting of erythrocyte ghost proteins. The reactions were done at pH 7 and 7.5. The reactants were separated by 10% SDS-PAGE, transferred to a PVDF membrane and probed with specific antibodies against human erythrocyte spectrin (1:500), band 3 (1:30000) and actin (1:1000) followed by horseradish peroxidase conjugated anti-human IgG (1:1000). The blots were developed with 4C1N. C, control without enzyme.

(60) VX-4 cleaved the majority of erythrocytic ghost proteins under acidic conditions (pH 5.0-6.0), whereas some activities were negligible at neutral pH (6.5-7.5). However, VX-2, VX-3, and VX-4 all degraded band-3 (anion exchanger 1, AE1) and actin at neutral pH. The proteolytic activities of VX-4 against erythrocyte actin and band-3 suggest an additional role for the protease in remodeling of erythrocyte cytoskeleton during the process of egress of merozoites from erythrocytes at the conclusion of the parasite erythrocytic cycle. Alternatively, actin degradation may be directly related to hemoglobin transport into the food vacuole, as a recent study showed that actin filament turnover in P. falciparum might be essential for both cytostome formation and hemoglobin translocation.

Example 8

Comparative Protein Structure Modeling

(61) Computational analyses were accomplished in a Silicon Graphics Octane 2 workstation, equipped with two parallel R12000 processors (SGI). Homology modeling was orchestrated within the SYBYL 6.9 COMPOSER module (Tripos Associates, MO). Energy minimization and molecular dynamic studies were performed with the DISCOVER module of InsightII 2000 (Accelrys). The geometrical and local environmental consistency of the model was assessed within the PROSTAT and InsightII 2000 Profiles-3D modules, together with the SYBYL 6.9 Matchmaker module. Structural models of FP-2, FP-3, VX-2, VX-3 and VX-4 mature domains were prepared on the basis of their sequence homology with several cysteine proteases using an analogous approach [Desai P V, Avery M A (2004) Structural characterization of vivapain-2 and vivapain-3, cysteine proteases from Plasmodium vivax: comparative protein modeling and docking studies. J Biomol Struct Dyn 21:781-790]. More than 35% sequence identity was observed between the protein homologs and the target AA sequence. The homologs used in this analysis included human cathepsins K (1ATK), V (1FH0) and S (1MS6); cruzain (1AIM), a cysteine protease from Ginger rhizome (1CQD) and actinidin (1AEC). Terms in parentheses refer to the Protein DataBank accession numbers for the corresponding crystal structures.

(62) Homology modeling of VX-4 demonstrated an overall topology similar to those of FP-2, FP-3, VX-2 and VX-3 with the average pairwise RMSD of 0.98 for the C atoms as above. However, a number of substitutions are recognized between VX-4 and the other VXs, including three prominent AA residues delineating the S2 pocket (Ala90, Gly154 and Glu180; numbering from the mature domain (SEQ ID NO: 2) of VX-4) (FIG. 4(A); see also box in FIGS. 1A, 1B, and 2). FIG. 4(A) shows superimposition of amino acid residues lining the binding pockets in VX-3 (cyan) and VX-4 (yellow). The residues are shown as sticks and the numbers of residues are indicated in reference to the corresponding enzymes.

Example 9

Mutation Analyses

(63) Site-directed mutagenesis was performed using a QuickChange II Site-Directed Mutagenesis Kit (Stratagene, Calif.). A pair of complementary primers with 39 bases was designed and a mutation to replace Ala90 to Ile (A90I), Gly154 to Ser (G154S) or Glu180 to Ala (E180A) was placed in the middle of the primers, as follows:

(64) TABLE-US-00003 PrimersformutationofAla90toIle(A90I) Forward: (SEQIDNO:7) 5-GGCTGCTTTGGTGGTTTAATCTCCCTTGCATTCGACGAC-3 Reverse: (SEQIDNO:8) 5-GTCGTCGAATGCAAGGGAGATTAAACCACCAAAGCAGCC-3 PrimersformutationofGly154toSer(G154S) Forward: (SEQIDNO:9) 5-GGCCCTCTCACCTTATCACTCACTGTGAATGATGATTTTTACG-3 Reverse: (SEQIDNO:10) 5-CGTAAAAATCATCATTCACAGTGAGTGATAAGGTGAGAGGGCC-3' PrimersformutationofGlu180toAla(E180A) Forward: (SEQIDNO:11) 5-GAAGAACCCAACCATGCAGTCATGATCGTGGGTTATGG-3 Reverse: (SEQIDNO:12) 5-CCATAACCCACGATCATGACTGCATGGTTGGGTTCTTC-3

(65) Parental DNA inserted in pQE-30 (Qiagen, Calif.) as prepared in Example 2 was amplified using Pfu Ultra HF DNA polymerase with these primers for 16 cycles in a DNA thermal cycler (Perkin-Elmer). After digestion of the parental DNA with Dpn I, the amplified DNA with nucleotide substitution was incorporated and transformed into E. coli XL1-Blue (Stratagene). The mutations were verified by DNA sequencing. Double and triple point mutagenesis of A90I, G154S, and E180A were also done as described above. Each mutant plasmid was transformed into competent E. coli M15 (pREP4) cells (Qiagen). Each recombinant protein was individually expressed, purified and refolded as described above.

(66) The substrate preferences of VX-4 were found to depend on AA residues occupying P2 site and thus, the diagnostic AA substitution might be relevant to the differential biochemistry of VX-4 compared to those of VX-2 and VX-3. Seven mutant forms of VX-4, in which these three AA residues were substituted by single, double, or triple site-directed mutagenesis (A90I, G154S, E180A, A90I/G154S, A90I/E180A, G154S/E180A, and A90I/G154S/E180A), were expressed in E. coli, and their proteolytic activities were examined as above. All of the refolded recombinant proteins showed hydrolytic activity against gelatin (FIG. 4(B)). Each mutant was expressed and analyzed by 12% SDS-PAGE (upper panel, FIG. 4(B), and by a gelatin substrate gel (lower panel, FIG. 4(B)).

(67) The results of assays against peptide substrates are shown in FIG. 4(C). In assays against peptide substrates, all of the mutants harboring A901 and G154S by single or multiple substitutions maintained the pH-dependent substrate specificity of wild-type VX-4. Conversely, those containing E180A lost activity against Z-RR-MCA at pH 7.5 (FIG. 4(C)). The activities of wild-type and mutant VX-4s were assayed in 100 mM sodium acetate (pH 5.5) against Z-LR-MCA and 100 mM Tris-HCl (pH 7.5) against Z-RR-MCA at 37 C. In each case, 10 mM DTT was supplemented in reaction buffer. Maximal activity was presented as 100%. MeanS.D. (n=3). These results demonstrate that Glu180 plays a key role in the pH-mediated switching of substrate specificity of VX-4.

(68) The impact of a single AA substitution at a critical position has been shown in a Leishmania major cathepsin B-like protease, in which a Gly residue at the putative S2 pocket provided no detectable proteolytic activity against Z-RR-AMC, while its replacement with Glu restored activity. A similar result was also observed for papain, which exhibited a preference for Phe over Arg at the P2 position, but exhibited cathepsin B-like specificity when the S2 subsite was altered. A cathepsin B-like cysteine protease of Giardia lamblia that harbors a Glu residue at the S2 pocket was active against both Z-FR-MCA and Z-LR-MCA. The crystallographic structure of cruzain, an essential cysteine protease of Trypanosoma cruzi, demonstrated that the side chain of Glu205 might vary positions and interact with different substrates according to pH and availability of an electrostatically appropriate partner in the S2 pocket. Therefore, the S2 subsite might be intimately involved in the determination of ligand specificity. Although most of the residues delineating the S2 pocket are hydrophobic, a polar residue is present at the pocket's hollow end in some cysteine proteases including VX-4 (FIGS. 1A and 1B; see also above). The S2 pocket of these enzymes might retain a negative charge at physiologic pH, allowing the capability to bind the polar guanadino group of Arg at the P2 position.

Example 10

Immunocytochemical Staining and Visualizing the Localization in Cells

(69) Thin blood smears (2 l) were prepared from EDTA-containing venipuncture blood immediately after sampling from patients infected with P. vivax (gift from Dr. J S Yeom). A part of the slides were stained with 3% Giemsa, rinsed and air dried. The unstained thin films were treated with 3% H.sub.2O.sub.2 for 5 min and incubated with 1% bovine serum albumin. The films were incubated with mouse anti-rVX-4 antibody (1:500 dilutions in PBS). The reactions were visualized with an avidin-biotin complex (DAKO, Carpentaria, Calif.) and examined under a light microscope (Axiophot, Carl Zeiss).

(70) The results reveal that VX-4 localizes densely within the food vacuoles and adjacent areas, as well as diffusely in the cytoplasm in erythrocytic Stages of P. vivax. The major hemoglobinases of P. falciparum (FP-2, FP-3 and plasmepsins) are targeted into a food vacuole through the ER-derived vesicles. However, it could not be clearly concluded whether the ER-derived, protease-containing vesicles are fuse with hemoglobin-containing transport vesicles, which were pinched off from cytosomes, or they directly contact with the food vacuole. However, investigations have been highly limited with the P. vivax proteins, not only due to low parasitemia in the patients' blood, but also due to failure of experimental maintenance of the parasite. The N-terminal regions of vavapains conserved the characteristic bipartite signal for trafficking to the food vacuole, which included cytoplasmic, transmembrane and lumenal motifs (FIGS. 1A and 1B). Alternatively, the proteolytic activities of VX-4 against erythrocytic actin and band-3 might suggest its additional role(s) in the remodeling of erythrocytic cytoskeleton, although the low activity with spectrin, which is one of the major cytoskeletal proteins in erythrocyte, makes it unclear (FIG. 5(D)).

(71) The inventors prepared a mouse antiserum specific to rVX-4, which showed negligible cross-reactions with rVX-2 and rVX-3 as well as erythrocyte proteins, as shown in FIG. 6(A). FIG. 6(A) shows rVX-2, rVX-3 and rVX-4 proteins, which were separated by 12% SDS-PAGE and transferred to PVDF membrane. The membrane was incubated with ant-rVX-4 (1:1000 dilutions) for 4 h, with an additional incubation with peroxidase-conjugated anti-mouse IgG (1:2000 dilutions) for 4 h. The blot was developed using 4C1N chromogen.

(72) The spatiotemporal expression pattern of VX-4 was also examined, and the results are shown in FIG. 6(B). In FIG. 6(B), a thin blood smear from a patient with vivax malaria was visualized by staining with anti-rVX-4 conjugated with avidin-biotin complex system. The protein was densely labeled in the food vacuoles and adjacent areas, as compared to the staining of the dark hemozoin pigment. As shown in FIG. 6(B), VX-4 was shown to be expressed through all of the intraerythrocytic stages of P. vivax, from ring to schizont/gametocyte stages. VX-4 localization appeared to be largely limited to the food vacuoles with dark homozoin pigment, while the protein was also labeled diffusely in the parasite cytoplasm. P. falciparum FP-3 seemed to have a biological implication(s), which is pivotal to the parasite's survival, in addition to the hemoglobin degradation and showed a distribution pattern similar to that of VX-4. Given the fact that VX-4 has hydrolytic activity against cytoskeletal proteins, the cytoplasmic distributions of VX-4 and FP-3 might suggest their cytosolic roles such as cytoskeletal remodeling and hemoglobin transportation, which is pivotal for the maintenance of intraerythrocytic stage of the parasites.

(73) The substrate specificity of proteases depends largely on interactions between a substrate and the enzyme active site. The binding efficiency is greatly affected by the physicochemical micromilieu. The reaction pH may confer substrate preference, as has been seen with cruzain. The pH-dependent substrate switching of VX-4 might be relevant to its multiple biological roles; the protein might function as a maturase of P. vivax plasmepsin 4 in the plasma membrane or cytosomes at neutral pH, while it participates in the degradation of hemoglobin in the acidic food vacuole. VX-4 might also be involved in cytoskeletal remodeling for the invagination of parasite plasma membrane to form cytostomes and/or the hydrolysis of host proteins to facilitate parasite egress from the erythrocyte. VX-4 thus may be a multifunctional enzyme, performing pivotal functions to ensure parasite survival during the complex life cycle of P. vivax. Given the multifunctional activities of VX-4, which are critical for the survival and/or metabolic homeostasis of the parasite, the enzyme might be an attractive target for the development of new antimalarial chemotherapeutics. Work toward further identification of natural substrates and distinct protease functions are currently underway to facilitate a more comprehensive understanding of the biological significance of this enzyme.

REFERENCES CITED

(74) All references cited herein are incorporated herein by reference in their entirety and for all purposes to the same extent as if each individual publication or patent or patent application was specifically and individually indicated to be incorporated by reference in its entirety for all purposes.

(75) The discussion of references herein is intended merely to summarize the assertions made by their authors and no admission is made that any reference constitutes prior art. Applicants reserve the right to challenge the accuracy and pertinence of the cited references.

(76) The present invention is not to be limited in terms of the particular embodiments described in this application, which are intended as single illustrations of individual aspects of the invention. Many modifications and variations of this invention can be made without departing from its spirit and scope, as will be apparent to those skilled in the art. Functionally equivalent methods and apparatus within the scope of the invention, in addition to those enumerated herein will be apparent to those skilled in the art from the foregoing description. Such modifications and variations are intended to fall within the scope of the appended claims. The present invention is to be limited only by the terms of the appended claims, along with the full scope of equivalents to which such claims are entitled.