Abstract
The present disclosure, according to one embodiment, describes a laboratory vessel for storing and treating a tissue sample comprises a closable opening, and additionally comprises a pipe part which is insertable in the opening of the laboratory vessel, wherein in inserted position, the pipe part seals against the laboratory vessel with its outer circumference and is clampingly displaceable with respect to said laboratory vessel, wherein a pestle is mounted in the laboratory vessel, over which the pipe part can be placed sealingly with its inner circumference. The laboratory vessel also comprises one or more tissue sample treatment agents. The tissue sample and the treatment agents are separated by placing the pipe part over the pestle, which separates a treatment agent region from a sample region.
Claims
1. A laboratory vessel for storing and treating a tissue sample (38), with a closable opening, comprising a pipe part (18), which is insertable through the opening of the laboratory vessel, wherein in inserted position, it seals against the laboratory vessel with its outer circumference and is clampingly displaceable with respect to said laboratory vessel (36), characterized by a pestle (40) mounted in the laboratory vessel, over which the pipe part (18) can be sealingly placed with its inner circumference.
2. The laboratory vessel according to claim 1, characterized in that the passage of the pipe part (18) is closed with a closure element (16).
3. The laboratory vessel according to claim 2, characterized in that the closure element (16) closes the pipe part (18) on the end which faces away from the pestle (40).
4. The laboratory vessel according to one of the claim 2 or 3, characterized in that the closure element (16) is a stopper.
5. The laboratory vessel according to one of the claim 2 or 4, characterized in that the closure element (16) is designed so as to be puncturable.
6. The laboratory vessel according to one of the previous claims, characterized in that the pestle (40) is mounted non-relocatably in the laboratory vessel.
7. The laboratory vessel according to one of the previous claims, characterized in that the end of the pestle (40) that faces in the direction of the opening of the laboratory vessel is arranged at a distance to the opening of the laboratory vessel.
8. The laboratory vessel of claim 1, comprising a treatment agent region located between the inner wall of the laboratory vessel (36) and the outer wall of the pestle (40), wherein at least one treatment agent 42 is arranged.
9. The laboratory vessel of claim 1, wherein a punch (44) is disposed above the laboratory vessel 36.
10. The laboratory vessel of claim 9, wherein the punch (44) is designed in a sleeve-like manner, with which the pipe part (18) can be displaced within the laboratory vessel (36).
11. A method for using the laboratory vessel of claim 1 to extract a tissue sample (38), wherein the extracted tissue sample (38) is at least initially not in contact with the one or more treatment agents (42).
12. The method of claim 8, further comprising processing the extracted tissue sample (38), wherein the extracted tissue sample (38) is brought in contact with one or more treatment agent (42).
Description
[0041] The invention is explained in detail by the drawings.
[0042] FIG. 1a shows a cross-sectional view of a part of an ear tag prior to punching;
[0043] FIG. 1b shows a cross-sectional view of an ear tag completely attached to the ear with a laboratory vessel according to the invention; and
[0044] FIGS. 2a-2e show cross-sectional views of the container according to the invention in different operating positions.
[0045] FIG. 1a shows a cross-sectional view of one of two parts of an ear tag, specifically a spike plate 10, having a spike 12 attached to it, which at its tip is detachably connected to a pipe part 18 by means of a separation surface 14 having, e.g. an adhesive. The tip of the spike 12 is designed to be on its spike head 28. In this embodiment, the pipe part 18 is designed as a pipe knife with a circular cutter 20. The circular cutter 20 is arranged on the edge of the pipe part 18 which faces away from the spike head 28. Opposite of the circular cutter 20, the pipe part 18 is closed with a closure element 16 designed in this case as closure wall. In this case, the closure element 16 is connected as one piece with the pipe part 18. As shown in FIG. 1a, a chamber 22, which is delimited by its wall, is formed within the pipe part 18, said chamber 22 being used, e.g. for receiving and retaining a tissue sample 38. The chamber 22 is enclosed by the outer wall of the pipe part 18 and is open in the region of the circular cutter 20. The circular cutter 20 thus forms a mouth 24, through which the chamber is accessible from outside.
[0046] FIG. 1b shows the arrangement according to FIG. 1a, after punching, attached to an ear 30. The ear tag part of FIG. 1a with the spike plate 10 and the spike 12 is connected to a second ear tag part, comprising a perforated plate 26 and a holder 34 arranged with the perforated plate.
[0047] As shown in FIG. 1b, the spike 12 is punched through the ear with its spike head 28 which carries the pipe part 18 and inserted through the perforated plate 26. As is shown in FIG. 1b, the perforated plate 26 is engaged in an annular groove 32 in the spike head 28. As a result, the plates 10 and 26 are securely connected to one another and to the ear 30.
[0048] At the punch point, the holder 34 is attached to the side of the perforated plate 26 which faces away from the spike plate 10, said holder 34 holding a laboratory vessel 36 according to the invention. As shown in FIG. 1b, during punching, the pipe part 18 was inserted in the laboratory vessel 36 and closes said vessel in the manner of a stopper. In this closed position, the container 36 can be detached from the holder 34, and the pipe part 18 can be detached from the spike head 28.
[0049] During punching, a sample 38 accumulated in the chamber 22 and which was formed by punching the ear 30 by means of the circular cutter 20. This sample can be further processed in the laboratory and particularly within the laboratory vessel. A design of the laboratory vessel 36 according to the invention is shown in five operating positions in FIGS. 2a through 2e.
[0050] FIG. 2a shows the laboratory vessel 36 according to the invention in an operating position, in which the pipe part 18as in FIG. 1ais inserted through the opening of the laboratory vessel 36 into said vessel's interior. In this design, the pipe part 18 is closed with a closure element 16 which is designed as stopper. The tissue sample 38 is held approximately centrally from the wall of the pipe part 18.
[0051] As can be seen when comparing FIGS. 2a through 2e, the pipe part 18 is displaceably mounted within the laboratory vessel 36. Furthermore, the pipe part 18 is designed so as to be sealing on its outer circumference with regard to the inner wall of the laboratory vessel 36. A rod-like pestle 40 is supported with a first end by the bottom of the laboratory vessel 36 and with its other end, which protrudes approximately axially into the interior of the laboratory vessel 36, is aligned to the pipe part 18. In a treatment agent region located between the inner wall of the laboratory vessel 36 and the outer wall of the pestle 40, a treatment agent 42 is arranged. One or more treatment agents 42 can be located here. Above the laboratory vessel 36, a punch 44 designed in a sleeve-like manner is shown, with which the pipe part 18 can be displaced within the laboratory vessel 36.
[0052] FIG. 2b shows the laboratory vessel 36 from FIG. 2a in a second operating position, in which the punch 44 is placed over the edge of the pipe part 18 which faces away from the pestle 40. FIG. 2c shows the laboratory vessel 36 from FIG. 2a in a next operating position, in which the punch 44 is inserted in the laboratory vessel 36. Through application of force by means of the punch 44, the pipe part 18 is displaced within the laboratory vessel 36 in the direction of the pestle 40 and placed over it. As can be seen, the pestle 40 pushes against the tissue sample 38 and squeezes it against the closure element 16 designed as stopper. In this operating position, the stopper 16 is already pushed out, for the most part, from the pipe part 18 by the application of force of the pestle 40 and the tissue sample 38. It is understood that in this embodiment of the laboratory vessel 36, the punch 44 has on its end which faces the pipe part 18 a receiving bore or the like, into which the closure element 16 and the tissue sample 38 can plunge when the pipe part 18 is punctured.
[0053] FIG. 2d shows the laboratory vessel 36 from FIG. 2a in a fourth operating position. Due to a further displacement of the punch 44 in the direction of the bottom of the laboratory vessel 36, the pipe part 18 is completely placed over the pestle 40. The pestle 40 has pushed the tissue sample 38 entirely through the pipe part 18. It can also be seen that the closure element 16 is completely removed from the pipe part 18. The region enclosing the tissue sample 38 is completely separated from the region enclosing the treatment agent 42. As can be seen in FIGS. 2c-2e, the tissue sample 38 is no longer enclosed by the treatment agent 42 or enclosed by only few parts of the treatment agent 42 after the pipe part 18 is placed over the pestle 40.
[0054] FIG. 2e shows the laboratory vessel 36 from FIG. 2a in a fifth operating position. It shows that the punch 44 was removed from the laboratory vessel 36, wherein the closure element 16, carried by the punch 44, was also removed from the laboratory vessel 36. It can be provided for the closure element 16 to be retained in a receptacle of the punch 44, for example by means of a catch mechanism or the like, when the pipe part 18 is displaced into the laboratory vessel 36. Instead of a catch mechanism, it is also possible that the closure element 16 is clampingly retained at its circumferential region, for example at its upper edge, in a receptacle of the punch 44.
[0055] In a modified design of the closure element 16, the closure element 16 is not removed from the laboratory vessel 36 by the punch 44 after the pipe part 18 is placed over the pestle 40 but, together with the tissue sample 38, remains within the laboratory vessel 36.
[0056] Taking into account the sealing function between pipe part 18, pestle 40, and inner wall of the laboratory vessel 36, it can be provided for all embodiments that at least one part of the chamber 22 of the pipe part 18 has an inner diameter that is greater than the outer diameter of the pestle 40. In this embodiment, the sealing between the pipe part 18 and the outer wall of the pestle 40 can be provided on the edge region of the pipe part 18 which faces the opening of the laboratory vessel 36. For that purpose, the edge of the pipe part 18 which faces the opening of the laboratory vessel 36 has a radially inwardly directed ring bead which seals with the pestle 40 when the pipe part 18 is placed over the pestle 40.
[0057] In FIGS. 1a and 1b, the circular cutter 20 is formed by an inwardly directed bevel of the wall of the pipe part 18, whereas the outer wall of the pipe part 18 runs axially parallel in the region of the circular cutter 20. In an alternative embodimentas in FIGS. 2a to 2ethe edge region of the pipe part 18 which faces the pestle 40 for forming a circular cutter is beveled only at the outer circumference of the pipe part. Here, the inner wall of the pipe part 18 runs axially parallel in the region of the circular cutter. Alternatively, it is easily also conceivable that an edge of the pipe part 18 for forming a circular cutter is beveled inwardly as well as outwardly.
[0058] The present disclosure describes methods for using one or more embodiments of the laboratory vessel of the invention, as variously described above, to extract a tissue sample (38), wherein the extracted tissue sample (38) is at least initially not in contact with the one or more treatment agents (42).
[0059] A method of the present invention, further comprises processing the extracted tissue sample (38), wherein the extracted tissue sample (38) is brought in contact with one or more treatment agent (42) located in the treatment agent location region.
LIST OF REFERENCE SIGNS
[0060] 10 Spike plate [0061] 12 Spike [0062] 14 Separation surface [0063] 16 Closure element [0064] 18 Pipe part [0065] 20 Circular cutter [0066] 22 Chamber [0067] 24 Mouth [0068] 26 Perforated plate [0069] 28 Spike head [0070] 30 Ear [0071] 32 Annular groove [0072] 34 Holder [0073] 36 Laboratory vessel [0074] 38 Tissue sample [0075] 40 Pestle [0076] 42 Treatment agent [0077] 44 Punch
