Automated airborne particulate matter collection, imaging, identification, and analysis

Abstract

The following is an apparatus and a method that enables the automated collection and identification of airborne particulate matter comprising dust, pollen grains, mold spores, bacterial cells, and soot from a gaseous medium comprising the ambient air. Once ambient air is inducted into the apparatus, aerosol particulates are acquired and imaged under a novel lighting environment that is used to highlight diagnostic features of the acquired airborne particulate matter. Identity determinations of acquired airborne particulate matter are made based on captured images. Abundance quantifications can be made using identity classifications. Raw and summary information are communicated across a data network for review or further analysis by a user. Other than routine maintenance or subsequent analyses, the basic operations of the apparatus may use, but do not require the active participation of a human operator.

Claims

1. A system for capturing images of particulate matter, the system comprising: a deposition surface, wherein the particulate matter is disposed on the deposition surface; an illumination apparatus comprising plurality of sources of electromagnetic radiation; a camera; and a control unit in communication with the camera and the plurality of sources of electromagnetic radiation, wherein the control unit is configured to execute instructions comprising: cycling the plurality of sources of electromagnetic radiation to illuminate the airborne particulate matter with a plurality of different wavelength combinations; and causing the camera to capture a plurality of images of the particulate matter, wherein each of the plurality of images corresponds with one of the plurality of different wavelength combinations emitted by the illumination apparatus.

2. The system of claim 1, wherein the plurality of sources of electromagnetic radiation are disposed in a ring configuration forming a pixel light ring, and wherein the pixel light ring illuminates the deposition surface with the plurality of sources of electromagnetic radiation.

3. The system of claim 2, wherein the pixel light ring further comprises one or more reflective light baffles, and wherein each of the one or more reflective light baffles is configured to increase an amount of light incident on the deposition surface.

4. The system of claim 2, wherein the plurality of images of the particulate matter comprises one or more obliquely lit images, and wherein the one or more obliquely lit images are captured by the camera when an adaxial orientation of the pixel light ring is disposed at an oblique angle relative to the deposition surface.

5. The system of claim 1, wherein the plurality of images of the particulate matter comprises one or more dark-field images.

6. The system of claim 1, wherein the instructions are such that cycling the plurality of sources of electromagnetic radiation comprises individually modulating each of the plurality of sources of electromagnetic radiation; and wherein at least a portion of the plurality of sources of electromagnetic radiation are tuned to emit electromagnetic radiation within a range from 200 nm to 800 nm.

7. The system of claim 6, wherein the plurality of sources of electromagnetic radiation further comprises one or more of: an ultraviolet source tuned to emit electromagnetic radiation within an ultraviolet waveband of the electromagnetic spectrum; or a near infrared source tuned to emit electromagnetic radiation within a near infrared waveband of the electromagnetic spectrum.

8. The system of claim 6, wherein each of the plurality of sources of electromagnetic radiation is tuned to emit electromagnetic radiation within the range from 200 nm to 800 nm.

9. The system of claim 1, wherein the plurality of sources of electromagnetic radiation comprises a red light emitting diode (LED), a green LED, and a blue LED.

10. The system of claim 1, wherein the plurality of images of the particulate matter comprises two or more different images each corresponding with a different adaxial illumination direction for illuminating the deposition surface with the illumination apparatus.

11. The system of claim 1, wherein the plurality of images of the particulate matter comprises two or more different images each corresponding with a different diffuse illumination direction for illuminating the deposition surface with the illumination apparatus.

12. The system of claim 1, wherein the plurality of images of the particulate matter comprises two or more different images each corresponding with a different illumination intensity emitted by the illumination apparatus on to the deposition surface.

13. The system of claim 1, wherein the control unit instructs the camera to capture two or more image frames for each of the plurality of different wavelength combinations.

14. The system of claim 13, wherein the two or more image frames corresponding with the same wavelength combination are averaged into a composite image.

15. The system of claim 14, wherein the composite image comprises reduced noise relative to each of the two or more image frames individually.

16. The system of claim 1, wherein the plurality of images is processed to infer topological features of the particulate matter based at least in part on image capture specifications.

17. The system of claim 16, wherein the image capture specifications comprise one or more of: a wavelength of electromagnetic radiation that was emitted by the illumination apparatus on to the deposition surface when an image was captured; an incident angle of one or more of the plurality of sources of electromagnetic radiation relative to the deposition surface when the image was captured; an intensity of light emitted by the illumination apparatus when the image was captured; or a frequency of electromagnetic radiation emitted by the illumination apparatus when the image was captured.

18. The system of claim 17, wherein the topological features of the particulate matter comprise one or more of a presence of pores within the particulate matter, a presence of furrows within the particulate matter, a presence of septae within the particulate matter, or a presence of mycelia fragments of spores within the particulate matter.

19. The system of claim 1, further comprising a linear focus apparatus comprising: a linear carriage comprising the camera and the plurality of sources of electromagnetic radiation; a screw stepper configured to translate the linear carriage; and a motor controller configured to translate the linear carriage to facilitate focusing of the plurality of images of the particulate matter.

20. The system of claim 19, wherein the control unit issues instructions to incrementally adjust a position of the linear carriage to achieve an optimal focus location for capturing the plurality of images of the particulate matter.

Description

BRIEF DESCRIPTION OF DRAWINGS

(1) (Note: this disclosure references components within each figure described below. The naming convention used throughout the specification is first to list the figure number, followed by a decimal point, followed by the specific component number of the given figure, prefaced by the word “Figure” or “Figures”, as the situation demands, and all included within parentheses, e.g., (x). For example, a reference to the illustration of the induction unit would be made as follows: (FIG. 1.2).)

(2) The features and advantages of the disclosure will become clearer with the following detailed description in connection with the accompanying drawings, wherein:

(3) FIG. 1 is a front-view illustration depicting an embodiment of a portion of the disclosure, with the following components identified:

(4) 1: ambient air

(5) 2: induction unit

(6) 3: airborne particulate inlet aperture

(7) 4: weather-resistant enclosure de-emphasized and indicated by a dashed line

(8) 5: air chamber

(9) 6: electrode (may be the anode)

(10) 7: an embodiment of airborne particulate matter

(11) 8: deposition surface (or medium of deposition)

(12) 9: translation or rotation mechanism

(13) 10: spacer tube—part of the perception unit

(14) 11: high-resolution magnified digital camera—part of the perception unit

(15) 12: pixel light ring—part of the perception unit

(16) 13: objective lens—part of the perception unit

(17) 14: linear focus apparatus part of the perception unit

(18) 15: main controller board and on-board computer with integrated Wi-fi communication capability

(19) 16: motor controllers

(20) 17: high-voltage electric field generator unit

(21) 18: filter

(22) 19: power and network cabling

(23) 20: sampling disk and with embedded electrode (may be the cathode). This component bears the deposition surface (FIGS. 1.8 and 3.8) and is the medium of deposition

(24) 24: environmental sensors

(25) 26: screw stepper.

(26) FIG. 2 is an illustration of a front oblique view some features have been omitted for clarity) depicting an embodiment of the disclosure, with the following components identified:

(27) 2: induction unit

(28) 3: airborne particulate inlet aperture

(29) 5: static charge air chamber and electrode

(30) 10: spacer tube

(31) 12: pixel light ring with reflective light baffles

(32) 13: objective lens

(33) 20: sampling disk and with embedded electrode (may be the cathode). This component bears the deposition surface (FIGS. 1.8 and 3.8)

(34) 21: cleaning mechanism electrode.

(35) FIG. 3 is an illustration of a back oblique view (some features have been omitted for clarity) depicting an embodiment of the disclosure, with the following components identified:

(36) 2: induction unit

(37) 3: airborne particulate inlet aperture

(38) 5: air chamber

(39) 6: electrode (may be the anode)

(40) 8: deposition surface

(41) 10: spacer tube

(42) 12: light pixel ring with reflective light baffles

(43) 13: objective lens

(44) 18: filter

(45) 20: sampling disk and with embedded electrodes

(46) 22: deposition surface cleaning area

(47) 23: cleaning brush

(48) 25: imaging area.

(49) FIG. 4 is an illustration of a rear oblique view (some features have been omitted for clarity) depicting an embodiment of the disclosure, with the following components identified:

(50) 2: induction unit

(51) 3: airborne particulate inlet aperture

(52) 9: translation or rotation mechanism

(53) 10: spacer tube

(54) 12: light pixel ring with reflective baffles

(55) 13: objective lens

(56) 14: focus mechanism comprising a linear rail, a motor and end-stops

(57) 18: fiber

(58) 20: sampling disk and with embedded electrode (may be the cathode). This component bears the deposition surface (FIGS. 1.8 and 3.8)

(59) 25: imaging area

(60) 26: screw stepper.

(61) FIG. 5 is an illustration of a front view depicting an embodiment of the disclosure, detailing the flow of particulates through the system with the following components identified:

(62) 1: an airborne particulate (enlarged and not to scale) enters the airborne particulate inlet aperture

(63) 2: electrostatic charge imparted to particulate(s)

(64) 3: particulate(s) deposited on deposition surface

(65) 4: illumination and imaging of particulate(s)

(66) 5: brush, airstream, electrostatic charge, gravity, and filter clean deposition surface.

(67) FIG. 6 A flowchart representing the steps of the analysis method for collecting, observing, and identifying airborne particulate matter dispersed in a gaseous medium with the following components identified:

(68) 1: collect airborne particulate matter onto the surface of a deposition medium

(69) 2: use an imaging device directed towards the medium of deposition to assess the locations and sizes of acquired particulates within the field of view, may be accomplished through image segmentation

(70) 3: determine the optimal focus location for each particulate using a focus assessment function limited to each particulate's segment boundary

(71) 4: capture one or more images from many different lighting configurations for each particle at the ideal local location

(72) 5: store captured images in a local or remote data repository for identity determination or other analysis.

BEST MODE FOR CARRYING OUT THE INVENTION

(73) The subject matter of this disclosure represents an automated, computerized, electro-mechanical apparatus (FIG. 1, FIG. 2, FIG. 3, FIG. 4, and FIG. 5) utilizing a combination of hardware and software to acquire, image, identify, quantify, and communicate resulting data describing airborne particulate matter (FIG. 1.7, FIG. 6) dispersed in a gaseous medium (FIG. 1.1). Other than installation, the continued supply of the requisite electrical power (FIG. 1.19), the continuity of a communication network (FIG. 1.19 and FIG. 1.15), and periodic maintenance to the apparatus, the operations of the disclosure described herein can have, but do not require, the presence or actions of a human operator. The typical composition of airborne particulate matter in ambient air is comprised of a complex mixture of dust, pollen grains, fungal spores, bacterial cells, viruses, by-products of internal engine combustion that comprise components of air pollution including hydrocarbons, and other compounds. The resulting data, which are the net product of the apparatus and its associated software, are images, identities, and quantifications of aerosol particulates. Such data may be used for a variety of purposes which include improving the diagnosis and avoidance of airborne particulate matter-related human health issues, plant reproduction, and other air pollution applications.

(74) The disclosure is comprised of the following components: a collection system (FIG. 1.2, FIG. 1.3, FIG. 1.5, FIG. 1.6, FIG. 1.8, and FIG. 1.20); a lighting and imaging system (FIG. 1.10, FIG. 1.11, FIG. 1.12, FIG. 1.13, and FIG. 1.14); a release and cleaning system (FIG. 1.2, FIG. 1.18, FIG. 2.21, FIG. 3.18, FIG. 3.22, and FIG. 3.23); and an analysis method (FIG. 6). Each component is described in greater detail below.

(75) The collection system enables the acquisition of airborne particulate matter from the ambient air. An embodiment of this disclosure may utilize an induction unit comprising, but not limited to, a blower fan (FIGS. 1,2, 2.2, 3.2 and 4.2) or other air-flow mechanism to draw ambient air (FIG. 1.1) from the atmosphere into the device through an aperture (FIGS. 1.3, 2.3, 3.3 and 4.3) that may project through an enclosure FIG. 1.4). The size of the aperture is not important except that it must be known for determining the volume of air flow for calibration and airborne particulate matter quantification purposes. After passing through the aperture (FIGS. 1.3, 2, 3, 3.3 and 4.3) and entering the device, the inducted air stream may enter into a larger venturi air chamber (FIGS. 1,5, 2.5, 3.5), and may pass a small electrode (FIGS. 1.6 and 3.6). The electrode may be negatively charged (an anode) and may act to increase the negative charge of passing airborne particulates (FIG. 1.7). A high-voltage electric field generator unit (FIG. 1.17) may create an electrostatic force and may also act to generate an oppositely charged field on a second electrode (a cathode) which may be situated under the deposition surface (FIG. 1.8 and FIG. 3.8). The electrostatic force created between the negatively charged airborne particulate matter and the positive electric field induced on the deposition surface may draw airborne particulates onto the deposition surface (FIG. 1.8 and FIG. 3.8), also referred to as the medium of deposition (FIG. 1.20), Periodically, a motor (FIGS. 1.9 and 4.9) or other translation or rotation mechanism (FIGS. 1.20, 2.20, 3.20 and 4.20) may engage to move the deposition surface such that additional airborne particulate matter may deposit on a different portion of the deposition surface (FIG. 1.8, FIG. 3.8, and FIG. 1.20). An embodiment of the disclosure may utilize a rotating disk with an embedded electrode (FIGS. 2.20, 3.20 and 4.20) that is oppositely charged relative to the airborne particulate matter and which may attract the airborne particulates to the edge of a disk (FIGS. 1.8, 3.8 and 5.3). The deposition surface is continuous, allowing the observation of airborne particulate matter to occur simultaneous with collection or after a delay following collection.

(76) In an embodiment of the disclosure, the collection system may temporarily shut off and discontinue acquisition of airborne particulate matter from the ambient air in the event of inclement weather conditions. Inclement weather may comprise stormy conditions with abnormally high levels of wind that may allow moisture or excessive levels of dust to enter the aperture (FIG. 1.3), information regarding the local weather or other environmental information used to determine control of the collection system may be retrieved over a communication network via a cable (FIG. 1.19), a Wi-Fi connection integrated with the onboard computer (FIG. 1.15), or from environmental sensors (FIG. 1.24) comprising air pressure, humidity, and temperature, integrated with the main controller board and on-board computer (FIG. 1.15).

(77) The lighting and imaging system enables the capture, recording, and storage of images of sufficient quality for analyses and identification of the acquired airborne particulate matter. In an embodiment of the disclosure, a motor (FIGS. 1.9 and 4.9) may rotate the deposition surface (FIG. 1.8, FIG. 3.8, and FIG. 1.20) and thus convey the acquired airborne particulate matter from the collection area to the imaging area (FIG. 3.25 and FIG. 4.25). The imaging area (FIG. 3.25 and FIG. 4.25) may be encased in glass to avoid air flow at the imaging location. An embodiment of this disclosure may utilize a finite objective lens (FIG. 1.13), a spacing tube (FIG. 1.10), and a high-resolution magnified camera (FIG. 1.11) or other optical or electronic sensor technology and an associated light/radiation system (FIG. 1.12, FIG. 2.12, and FIG. 3.12) to capture and record digital representations of the acquired airborne particulate matter. The objective lens (FIG. 1.13), spacing tube (FIG. 1.10), a high-resolution magnified camera (FIG. 1.11) or other electronic/optical sensor technology, and associated light/radiation system (FIG. 1.12, FIG. 2.12, and FIG. 3.12) comprise the perception unit for human or machine observation of the airborne particulate matter. Magnification of the perception unit may be accomplished via optical means, digital means or both and may comprise an objective lens or series of lenses (FIG. 1.13), a spacing tube (FIG. 1.10), a high-resolution magnified camera (FIG. 1.11), or other optical or electronic sensor technology. The field of view of the objective lens (FIG. 1.13, FIG. 2.13, FIG. 3.13 and FIG. 4.13) may be recorded with a digital camera (FIG. 1.11 and FIG. 4.11) or other electronic/optical sensor technology that may be oriented so as to examine the deposition surface (FIG. 1.8, FIG. 3.8, and FIG. 1.20) and the acquired airborne particulate matter thereon.

(78) For lighting, an embodiment of this disclosure may use multiple light and electromagnetic radiation sources, individually controlled, and situated in a ring (FIG. 1.12) surrounding the acquired airborne particulate matter on the deposition surface (FIG. 1,8, FIG. 3.8, and FIG. 1.20). The lighting apparatus may utilize red-green-blue (RGB) light emitting diodes (LED) or other electromagnetic radiation sources similarly situated within the pixel light ring (FIG. 1.12) to illuminate the deposition surface (FIG. 1.8, FIG. 3.8, and FIG. 1.20) within the field of view of the objective lens (FIG. 2.13, FIG. 3.13, and FIG. 4.13) with ultraviolet, visible, and/or near infrared electromagnetic light at a high angle of incidence onto the top adaxial surface of the airborne particulate matter. An embodiment of the disclosure may also include reflective light baffles on the pixel light ring (FIG. 3.12 and FIG. 4.12) to increase the amount of diffuse light in addition to direct light incident to the acquired airborne particulate matter on the deposition surface (FIG. 1.8, FIG. 3.8, and FIG. 1.20). A control unit comprised of a software system, which may be integrated with the main controller board (FIG. 1.15), may individually modulate and rotate the LED pixels through a variety of wavelength combinations from 200 to 800 nanometers and lighting perspectives while the software may cause images of the acquired airborne particulate matter to be captured under each lighting combination in order to obtain a series of obliquely lit, dark-field images captured from different adaxial and diffuse illumination directions, over a range of the electromagnetic frequencies, and across a range of illumination intensities. Obliquely lit and dark-field refer to the adaxial orientation of pixel light ring (FIG. 1.12) and the high-resolution magnified digital camera (FIG. 1.11) relative to the acquired airborne particulate matter on the deposition surface (FIG. 1.8, FIG. 3.8, and FIG. 1.20) which is illuminated with light and other electromagnetic radiation both on its top surface and/or diffusely. To reduce camera noise, multiple camera frames may be captured of each perspective and lighting combination, and the frames may be averaged together into a composite image. Knowledge of the direction, frequency, and intensity of the light source may be used to highlight and infer topological features of the acquired airborne particulate matter including, pores and furrows of pollen grains and septae or mycelia fragments of spores.

(79) In an embodiment of this disclosure, the imaging system may have a linear focus apparatus (FIG. 1.14) which may allow the software to seek out ideal focus on a particulate-specific basis. The high-resolution magnified digital camera (FIG. 1.11), the spacer tube (FIG. 1.10), the objective lens (FIG. 1.13), or the pixel light ring (FIG. 1.12) may ride on a linear carriage (FIGS. 1.14 and 4.14) to facilitate focusing, winch carriage may be translated via a screw stepper (FIG. 1.26 and FIG. 4)) controlled by a motor controller (FIG. 1.16). Focus may be accomplished in two phases: coarse and fine. Using the digital camera (FIG. 1.11), the computer control system may assess the best focus for the overall field of view (the coarse focus) by incrementally advancing the focus motor (FIG. 4.14) and processing the image through a focus assessment function. Such focus function may consist of bilinear squared difference factored with the overall histogram width. Once the optimal focus location is determined for the overall field of view, image segmentation algorithms (described below) may be used to determine individual particulate locations and sizes. A fine focus process may then be performed whereby the focal point may be translated through a range proximal to the overall optimal. Through this process, the software may take multiple image samples constrained to the boundaries of each segment and at ranged focal positions. The optimal focus position for each segment region may then be determined by taking the best of the focus values for that region. This focus position may then be used as the focus position in the subsequent imaging of each region.

(80) The release and cleaning system enables the evacuation and discharge of the acquired airborne particulate matter front the deposition surface (FIG. 1.8, FIG. 3.8, and FIG. 1.20). Once a portion of the deposition surface (FIG. 1.8, FIG. 3.8, and FIG. 1.20) has been imaged, it may be cleaned before being rotated or translated back into the collection area near the airborne particulate inlet aperture (FIG. 1.3, FIG. 2.3, FIG. 3.3, and FIG. 4.3). Release and cleaning of airborne particulate matter may be accomplished via one or more of the following mechanisms or a combination thereof: reversing the electric charge; airflow; mechanical; physical; gravity; or filter. In an embodiment of the disclosure, the deposition surface (FIG. 1,8, FIG. 3.8, and FIG. 1.20) may be rotated or otherwise translated into a region (FIG. 3.22) where oppositely charged electrodes (FIG. 2.21), a foam or brush cleaning mechanism (FIG. 3.23), passing air being evacuated from the device which may be from the action of the induction unit (FIGS. 1.2, 2.2, 3.2 and 4.2), and gravity may combine to remove the acquired airborne particulate matter and carry it or let it fall passively into a filter (FIGS. 1.18, 3.18, 4.18 and 5.5).

(81) In an embodiment of the disclosure, an electric field may be utilized to repel the acquired airborne particulate matter front the deposition surface (FIG. 1.8, FIG. 3.8, and FIG. 1.20). The creation of an electric field of opposite polarity to that used for collection, between an electrode beneath the deposition surface (FIG. 1.8, FIG. 3.8, and FIG. 1.20) and another electrode (FIG. 2.21), which may be a plate or ring beneath an air filter (FIGS. 1.18 and 3.18), may result in a strong repulsive force being exerted on to the acquired airborne particulate matter located on the deposition surface (FIG. 1.8, FIG. 3.8, and FIG. 1.20). Air flow may additionally be concentrated on the cleaning area (FIG. 3.22) generating an additional force due to a relative difference of atmospheric pressure on the acquired airborne particulate matter located on the deposition surface (FIG. 1.8, FIG. 3.8, and FIG. 1.20) and creating an additional cleaning effect. Furthermore, a charged or uncharged piece of foam, brush, sponge, stopper, or other physical object (FIG. 3.23) may be used to generate a physical force on the acquired airborne particulate matter located on the deposition surface (FIG. 1.8, FIG. 3.8, and FIG. 1.20) and mechanically remove persistent acquired airborne particulate matter located on the deposition surface (FIG. 1.8, FIG. 3.8, and FIG. 1.20). In an embodiment of the disclosure, the deposition surface cleaning area (FIG. 3.22) may be oriented in such a way that a gravitational force acts on the acquired airborne particulate matter located on the deposition surface (FIG. 1.8, FIG. 3.8, and FIG. 1.20), encouraging the acquired airborne particulate matter to drop away from the deposition surface (FIG. 1.8, FIG. 3.8, and FIG. 1.20) within the deposition surface cleaning area (FIG. 3.22) and may be in combination with the exertion of an electrostatic force, a force created from the relative difference of atmospheric pressure, and physical force. In an embodiment of this disclosure, the electrostatic, difference in atmospheric pressure, physical, and gravitational forces represent separate removal mechanisms and may all be present individually or in combination within a concentrated area (FIG. 3.22) to give maximal cleaning and avoid contaminating of the deposition surface (FIG. 1.8, FIG. 3.8, and FIG. 1.20) during sampling collection cycles.

(82) The analysis method of this disclosure (FIG. 6) enables the determination of the identity of acquired airborne particulate matter. The images acquired by an embodiment of this disclosure may be processed partially or completely by the computer processor within the disclosure or may be transmitted to off-site servers for partial or complete processing. Image processing may comprise three dimensional (3D) reconstruction from lighting, image compositing, correction, or other processing to enhance image quality including, but not limited to, histogram equalization, sharpness enhancement, and edge detection. Images may be analyzed manually by a human, via automated counting algorithms, or both. After analysis, the images may be kept in a storage repository for later analysis, or may be discarded. In an embodiment of this disclosure, the device may send raw imagery to a cloud service wirelessly or over a network cable (FIG. 1.19), but may also run a neural network-based algorithm (described below) trained to recognize various identities of acquired airborne particulate matter including species, genera, or family of pollen grains; species, genera, or family of fungal spores; species, genera, or family of bacterial cells; species of internal engine combustion by-products; or the identity of other airborne particulate matter.

(83) An embodiment of this disclosure may identify acquired airborne particulate matter in the captured digital images by using image segmentation algorithms, known informally as “blobbing” techniques. Maximally Stable Extremal Regions (MSER) {13} and other algorithms produce a series of regions pertaining to each identified feature. Each identified acquired airborne particulate matter feature may be known as a segment. For segments larger than a configured threshold, the segment's image may be passed into a proximal classifier function which may determine whether the segment is most likely to be a) a single simple object; b) a single compound object; or c) a cluster of objects. If the segment appears to be a cluster, a declustering function utilizing concavity and seam based splitting may iteratively break overlapping segments into a series of masked out sub-segments. The classifier function thus described may be necessary to identify certain airborne particulate matter such as pollen which may have compound features, resulting in concavities.

(84) For each segment (acquired airborne particulate matter features identified by the MSER or similar algorithm), or group of segments if there are multiple acquired airborne particulate matter features located at the same optimal focus location, an embodiment's software may step to said focus position and engage the lighting and imaging system described above. The lighting and capture system involves sequentially turning on LED lights (FIGS. 1.12, 2.12, 3.12 and 4.12) and capturing images lighted under various illumination intensities and from various illumination perspectives. RGB LED lights may be strategically placed on a reflective surface (FIGS. 1.12, 2.12, 3.12 and 4.12) around the perimeter of the objective lens (FIG. 1.13 and FIG. 2.13) and may be individually controlled to illuminate the acquired airborne particulate matter from a multitude of perspectives, light intensity levels, and wavelengths. Acquired airborne particulate matter may be sequentially imaged with oblique lighting from multiple vantage points, highlighting the features, topology, transparency, and absorption at light wavelengths from 200 to 800 nanometers.

(85) In an embodiment of the disclosure, multiple obliquely lit image segments may be used to infer three dimensional (3D) shape characteristics and surface features of the acquired airborne particulate matter. Light transmissibility and reflectivity may also be inferred using this multiple lighting angle approach. To infer 3D features, a software model may be constructed starting with a malleable primitive object, a sphere may be used as a reasonable malleable primitive object. The model may be scaled to match the maximal extent of the aggregate captured image and may then be sculpted inward based on the perimeter shape. Highlights from each directionally lit image may be then used to push or pull portions of the model according to the 3D vector of the particular light. If the object's facing surface is determined to be convex, highlights that appear on the side opposite the light may be treated as being on the far end of the translucent object, thus shaping may be possible on both the facing and opposing sides. Once a 3D representation of the object is constructed, its position may be normalized, a color or texture is applied to it based on the captured image(s) of the acquired airborne particulate matter, and may be rendered with high-contrast lighting. The resulting rendering may be composed with the original image, or may be used for direct observation. Alternatively, the 3D representation may serve as input to a classifier that is suitable for working with 3D models.

(86) An embodiment of this disclosure may implement machine vision to recognize and classify acquired airborne particulate matter via a neural network classifier. Prior to classification, various image pre-processing commonly used in machine vision may be applied, comprising histogram equalization, sharpness enhancement, and edge detection. Neural networks may be generally defined by a set of interconnected input “neurons”. The connections may have numeric weights that can be tuned based on experience, making the neural network adaptive to inputs and thus capable of learning. The characterization algorithms may activate and weight neurons by the pixel values of an individual input image. After initial weighting, the values may be passed to other neurons where they may be transformed b other functions relative to a library of identification criteria and then may be passed on again to other neurons. This process may be iterative and may repeat until the output neuron is activated and classification probabilities are achieved. The resulting probabilities may be further weighed and identification may be achieved using a statistical model of spatial and temporal abundance of the airborne particulate matter, so that a particular acquired airborne particulate matter such as a pollen grain that may be deemed by the classifier to be equally likely to be either of two genera, will be weighted towards the genera that is most likely for that location and time of year,

(87) Segment images and associated analysis data may be, in an embodiment of this disclosure, available online to those authorized to access them. In addition to displaying the images, a human operator may be able to give feedback regarding classification, which feedback and corrections may be used to improve the training of the classifier. The series of segmentation images, comprising the digital imagery or particle topology inputs as well as initial particle determinations, may be used directly or with further processing as inputs to classification software or may be analyzed by a human, either remotely or locally, to produce airborne particulate matter identity determinations. The statistical, spatial and temporal model may also be improved, using data coming from devices, as well as correctional feedback from software users.

(88) In an embodiment of the disclosure, captured images may be processed by the onboard computer (FIG. 1.15), and the resulting images and data may be stored until internet communication is established with a remote network storage system via a cable (FIG. 1.19) or Wi-Fi connection integrated with the onboard computer (FIG. 1.15). The computer processor within the disclosure (FIG. 1.15) or off-site cloud servers may make the captured images and count results available online and via push notification. Quantification of the acquired airborne particulate matter based on the time each portion of the deposition surface (FIG. 1.8, FIG. 3.8, and FIG. 1.20) was actively sampled and collected may be accomplished and included with the results.

(89) The disclosure has been described in an illustrative manner, and it is to be understood that the terminology which has been used is intended to be in the nature of words of description rather than of limitation. Obviously, many modifications and variations of the present disclosure are possible in light of the above teachings. For example, the device could include mechanisms to direct air currents strategically or to protect itself from adverse environmental and weather conditions. Embodiments may use various deposition media on which to collect particulates, various methods for positioning the particulates for imaging, and various methods of lighting and imaging specimens. Embodiments may also implement practical variants by allowing users flexibility in the kinds of enclosures and mounting mechanisms.

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