Enhancing the antimicrobial activity of biocides with polymers

Abstract

Disclosed are compositions comprising a) an antimicrobial agent, which is selected from the group consisting of biocides containing halogen atoms and/or containing phenolic moieties, formic acid, chlorine dioxide, chlorine dioxide generating compounds, dialdehydes; components containing an antimicrobial metal such as antimicrobial silver, and b) a polyamine, especially a polyethylenimine. The polyamine is effective as a booster for the antimicrobial agent.

Claims

1. A composition comprising: a) 0.05 to 1% b.w. of an antimicrobial agent, which is 2-bromo-2-nitropropane-1,3-diol, b) 10 to 1000 ppm of a polyethyleneimine, which is an oligomer or polymer grafted with 0.01 to 100 parts by weight of ethylene oxide on 1 part by weight of the polyethyleneimine, c) water, and d) 3.5 to 80% b.w. of a surfactant comprising at least one of an anionic surfactant, a cationic surfactant, a nonionic surfactant, and an amphoteric surfactant, relative to the total weight of the composition; wherein: the polyethylenimine and the 2-bromo-2-nitropropane-1,3-diol form a synergistic mixture in which the polyethylenimine enhances antimicrobial activity of the 2-bromo-2-nitropropane-1,3-diol against at least one of Pseudomonas aeruginosa and Staphylococcus aureus, the composition comprises per 1 part by weight of the antimicrobial agent (a) 0.01 to 1 parts by weight of the polyethyleneimine (b), the composition is in the form of an aqueous composition, and the composition is free from isothiazolinones.

2. The composition of claim 1, wherein the polyethyleneimine has a number-average molecular weight in the range from 500 to 2 000 000 g/mol.

3. The composition according to claim 1, comprising at least one further component selected from the group consisting of hydrotropic agents, further additives that improve the antimicrobial effect, and agents stabilizing the antimicrobial agent (a) and/or the polyethyleneimine (b) in the formulation.

4. A method of manufacturing a home care formulation or a personal care formulation, the method comprising: adding the composition of claim 1 to the home care formulation or the personal care formulation; wherein: the home care formulation is selected from the group consisting of a disinfectant, all purpose cleaner, laundry detergent, dishwashing liquid, deodorant, fabric conditioner, product for disinfection and sanitization of hard surfaces, floor cleaner, glass cleaner, kitchen cleaner, bath cleaner, sanitary cleaner, hygiene rinse product for fabrics, carpet cleaner, furniture cleaner, or a product for conditioning, sealing, caring or treating hard and soft surfaces; and the personal care formulation is selected from the group consisting of a deodorant, skin care preparation, bath preparation, cosmetic care preparation, foot-care preparation, light-protective preparation, skin-tanning preparation, depigmenting preparation, insect-repellent, antiperspirant, preparation for cleansing and caring for blemished skin, hair-removal preparation, shaving preparation, fragrance preparation, cosmetic hair-treatment preparation, antidandruff preparation and oral care composition.

5. The composition according to claim 1, wherein the water is present in an amount of at least 57 wt. % relative to the composition.

6. The composition according to claim 1, wherein the surfactant comprises the anionic surfactant.

7. The composition according to claim 1, wherein the surfactant comprises the cationic surfactant.

8. The composition according to claim 1, wherein the surfactant comprises the nonionic surfactant.

9. The composition according to claim 1, wherein the surfactant comprises the amphoteric surfactant.

10. The composition according to claim 1, wherein the composition is in the form of a solution.

11. The composition according to claim 1, wherein the composition comprises 0.1 to 1% b.w. of the antimicrobial agent (a).

12. The composition according to claim 1, wherein the composition comprises 50 to 1000 ppm of the polyethyleneimine (b).

13. The composition according to claim 1, wherein the composition comprises 80 to 1000 ppm of the polyethyleneimine (b).

14. The composition according to claim 13, wherein the composition comprises 0.1 to 1% b.w. of the antimicrobial agent (a).

Description

FORMULATION EXAMPLES

(I) all Purpose Cleaner Providing Antibacterial Protection

(1) TABLE-US-00007 INCI Name/ Chemical Trade name name Supplyer % w/w % w/w % w/w % w/w % w/w % w/w % w/w % w/w % w/w Part A Biocide 0.001-5% 0.001 0.01 0.1 5 0.001 0.01 0.1 5 1 PEI (I), (II), 0.001 0.001 0.001 0.001 2 2 2 2 1 (III), (IV) Part B Plurafac LF 400 Alkoxylated BASF 10.00 10.00 10.00 10.00 10.00 10.00 10.00 10.00 10.00 Alcohol Hostapur SAS 60 Sodium C14-17 Clariant 14.00 14.00 14.00 14.00 14.00 14.00 14.00 14.00 14.00 Alkyl Sec Sulfonate Texapon ® LS 35 Lauryl-myristyl BASF 5.00 5.00 5.00 5.00 5.00 5.00 5.00 5.00 5.00 sulfate Na-salt Diethylene Glycol Diethylene Glycol Merck 5.00 5.00 5.00 5.00 5.00 5.00 5.00 5.00 5.00 Monobutyl Ether Monobutyl Ether Part C Perfume and qs qs qs qs qs qs qs qs qs preservative Citric Acid Citric Acid Merck ad ad ad ad ad ad ad ad ad pH 6.5 pH 6.5 pH 6.5 pH 6.5 pH 6.5 pH 6.5 pH 6.5 pH 6.5 pH 6.5 IRAGON ® Blue Acid Blue BASF 0.01 0.01 0.01 0.01 0.01 0.01 0.01 0.01 0.01 ABL 9 sol. 1% Cibafast ® H Sodium Benzo- BASF 0.05 0.05 0.05 0.05 0.05 0.05 0.05 0.05 0.05 Liquid triazolyl Butylphenol Sulfonate (and) Buteth-3 (and) Tributyl Citrate Part D Water Aqua qs qs qs qs qs qs qs qs qs to 100 % w/w: wt% as supplied

(II) all Purpose Cleaner, Providing Strong Immediate Bactericidal and Long Lasting Effects on Treated Surface

(2) TABLE-US-00008 INCI Name/ Chemical Trade name name Supplyer % w/w % w/w % w/w % w/w % w/w % w/w % w/w % w/w % w/w Part A Biocide 0.001 0.01 0.1 5 0.001 0.01 0.1 5 1 PEI (I), (II), 0.001 0.001 0.001 0.001 2 2 2 2 1 (III), (IV) Part B Tinosan ® HP 100 Hydroxydichloro- BASF 0.30 0.30 0.30 0.30 0.30 0.30 0.30 0.30 0.30 diphenyl Ether Part C Texapon ® LS 35 Lauryl-myristyl BASF 10.00 10.00 10.00 10.00 10.00 10.00 10.00 10.00 10.00 sulfate Na-salt Diethylene Glycol Diethylene Glycol Merck 5.00 5.00 5.00 5.00 5.00 5.00 5.00 5.00 5.00 Monobutyl Ether Monobutyl Ether Glucopon ® 215 Alkyl BASF 3.00 3.00 3.00 3.00 3.00 3.00 3.00 3.00 3.00 CS UP Polyglycoside Part D Perfume and qs qs qs qs qs qs qs qs qs preservative Citric Acid Citric Acid Merck ad ad ad ad ad ad ad ad ad pH 3.0 pH 3.0 pH 3.0 pH 3.0 pH 3.0 pH 3.0 pH 3.0 pH 3.0 pH 3.0 Water Aqua qs qs qs qs qs qs qs qs qs to 100 to 100 to 100 to 100 to 100 to 100 to 100 to 100 to 100

(III) all Purpose Cleaner, Providing Bactericidal and Long Lasting Effects on Treated Surfaces

(3) TABLE-US-00009 INCI Name/ Chemical Trade name name Supplyer % w/w % w/w % w/w % w/w % w/w % w/w % w/w % w/w % w/w Part A Biocide 0.001 0.01 0.1 5 0.001 0.01 0.1 5 1 PH (I), (II), 0.001 0.001 0.001 0.001 2 2 2 2 1 (III), (IV) Part B Plurafac LF 400 Alkoxylated BASF 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 Alcohol Sodium Cumene Sodium Cumene Hickson 5.00 5.00 5.00 5.00 5.00 5.00 5.00 5.00 5.00 Sulfonate Sulfonate Manro Marlon PS 65 Sodium C13-17 Sasol 10.0 10.0 10.0 10.0 10.0 10.0 10.0 10.0 10.0 Alkane Sulfonate Part C Perfume and q q q q q q q q q Preservative Water Aqua qs qs qs qs qs qs qs qs qs to 100 to 100 to 100 to 100 to 100 to 100 to 100 to 100 to 100 Trisodium Trisodium Fluka 5.00 5.00 5.00 5.00 5.00 5.00 5.00 5.00 5.00 Citrate Citrate Dihydrate Dihydrate

(IV) all Purpose Cleaner and Disinfectant, Provides Strong Immediate Bactericidal and Long Lasting Effects on Treated Surfaces Even at Low Concentrations

(4) TABLE-US-00010 INCI Name/ Chemical Trade name name Supplyer % w/w % w/w % w/w % w/w % w/w % w/w % w/w % w/w % w/w Part A Biocide 0.001 0.01 0.1 5 0.001 0.01 0.1 5 1 PEI (I), (II), 0.001 0.001 0.001 0.001 2 2 2 2 1 (III), (IV) Part B Glucopon ® 215 Alkyl BASF 3.00 3.00 3.00 3.00 3.00 3.00 3.00 3.00 3.00 CS UP Polyglycoside Texapon ® LS 35 Lauryl-myristyl BASF 10.00 10.00 10.00 10.00 10.00 10.00 10.00 10.00 10.00 sulfate Na-salt Sodium Cumene Sodium Cumene Hickson 5.00 5.00 5.00 5.00 5.00 5.00 5.00 5.00 5.00 Sulfonate Sulfonate Manro 2-Propanol Isopropyl Merck 10.00 10.00 10.00 10.00 10.00 10.00 10.00 10.00 10.00 Alcohol Part C Perfume and qs 5.00 5.00 5.00 5.00 5.00 5.00 5.00 5.00 Preservative Water Aqua qs qs qs qs qs qs qs qs qs to 100 to 100 to 100 to 100 to 100 to 100 to 100 to 100 to 100 Trisodium Trisodium Fluka 5.00 5.00 5.00 5.00 5.00 5.00 5.00 5.00 5.00 Citrate Citrate Dihydrate Dihydrate

(V) Bactericidal all Purpose Cleaner, Providing Strong Immediate Bactericidal and Long Lasting Effects on Treated Surfaces

(5) TABLE-US-00011 INCI Name/ Chemical Trade name name Supplyer % w/w % w/w % w/w % w/w % w/w % w/w % w/w % w/w % w/w Part A Biocide 0.001 0.01 0.1 5 0.001 0.01 0.1 5 1 PEI (I), (II), 0.001 0.001 0.001 0.001 2 2 2 2 1 (III), (IV) Part B Texapon ® LS 35 Lauryl-myristyl BASF 10.00 10.00 10.00 10.00 10.00 10.00 10.00 10.00 10.00 sulfate Na-salt Glucopon ® 215 Alkyl BASF 3.00 3.00 3.00 3.00 3.00 3.00 3.00 3.00 3.00 CS UP Polyglycoside Sodium Cumene Sodium Cumene Hickson 5.00 5.00 5.00 5.00 5.00 5.00 5.00 5.00 5.00 Sulfonate Sulfonate Manro Part C Perfume and qs qs qs qs qs qs qs qs qs Preservative Citric Acid Citric Acid Merck ad ad ad ad ad ad ad ad ad pH 3 pH 3 pH 3 pH 3 pH 3 pH 3 pH 3 pH 3 pH 3 Water Aqua qs qs qs qs qs qs qs qs qs to 100 to 100 to 100 to 100 to 100 to 100 to 100 to 100 to 100

(VI) Anti-Bacterial Fabric Softener Based on Conventional Quat

(6) TABLE-US-00012 INCI Name/ Chemical Trade name name Supplyer % w/w % w/w % w/w % w/w % w/w % w/w % w/w % w/w % w/w Part A Biocide 0.001 0.01 0.1 5 0.001 0.01 0.1 5 1 PEI (I), (II), 0.001 0.001 0.001 0.001 2 2 2 2 1 (III), (IV) Part B Neodol 25-7 E C12-15 Pareth-7 Shell 0.50 0.50 0.50 0.50 0.50 0.50 0.50 0.50 0.50 Chemicals Arquad 2HT-75 Quaternium 18 Akzo 4.00 4.00 4.00 4.00 4.00 4.00 4.00 4.00 4.00 (and) Isopropyl Nobel Alcohol Part D Water Aqua qs qs qs qs qs qs qs qs qs Perfume and qs qs qs qs qs qs qs qs qs Preservative

(VII) Anti-Bacterial Fabric Softener Based on Esterquat

(7) TABLE-US-00013 INCI Name/ Chemical Trade name name Supplyer % w/w % w/w % w/w % w/w % w/w % w/w % w/w % w/w % w/w Part A Biocide 0.001 0.01 0.1 5 0.001 0.01 0.1 5 1 PEI (I), (II), 0.001 0.001 0.001 0.001 2 2 2 2 1 (III), (IV) Part B Rewoquat WE 18 Dihydrogenated Evonik 4.00 0.50 0.50 0.50 0.50 0.50 0.50 0.50 0.50 Tallowethyl Hydroxyethyl- amonium Neodol 25-7 E C12-15 Pareth-7 Shell 0.50 4.00 4.00 4.00 4.00 4.00 4.00 4.00 4.00 Chemicals Antifoam DB 31 Dow 0.10 qs qs qs qs qs qs qs qs Corning Part D Water Aqua qs qs qs qs qs qs qs qs qs to 100 to 100 to 100 to 100 to 100 to 100 to 100 to 100 to 100 Perfume and qs qs qs qs qs qs qs qs qs Preservative

(VIII) Antibacterial Heavy Duty Liquid Detergent

(8) TABLE-US-00014 INCI Name/ Chemical Trade name name Supplyer % w/w % w/w % w/w % w/w % w/w % w/w % w/w % w/w % w/w Part A Biocide 0.001 0.01 0.1 5 0.001 0.01 0.1 5 1 PEI (I), (II), 0.001 0.001 0.001 0.001 2 2 2 2 1 (III), (IV) Part B Marlon A 375 Sodium Sasol 15.00 15.00 15.00 15.00 15.00 15.00 15.00 15.00 15.00 Dodecylbenzene- sulfonate Neodol 45-7 E C14-15 Pareth-7 Shell 14.00 14.00 14.00 14.00 14.00 14.00 14.00 14.00 14.00 Chemicals Alcohol SD Alcohol 39-C Berkel 9.00 9.00 9.00 9.00 9.00 9.00 9.00 9.00 9.00 Part C Soap Base Sodium Mettler 10.00 10.00 10.00 10.00 10.00 10.00 10.00 10.00 10.00 Tallowate (and) Sodium Cocoate Trisodium Trisodium Fluka 4.00 4.00 4.00 4.00 4.00 4.00 4.00 4.00 4.00 Citrate Citrate Dihydrate Dihydrate Triethanolamine Triethanolamine BASF 5.00 5.00 5.00 5.00 5.00 5.00 5.00 5.00 5.00 Part D Tinopal ® CBS-X Fluorescent BASF 0.10 0.10 0.10 0.10 0.10 0.10 0.10 0.10 0.10 Whitening Agent (FWA) Part E Water Aqua qs qs qs qs qs qs qs qs qs to 100 to 100 to 100 to 100 to 100 to 100 to 100 to 100 to 100

(IX) Anti-Bacterial Heavy Duty Liquid Detergent

(9) TABLE-US-00015 INCI Name/ Chemical Trade name name Supplyer % w/w % w/w % w/w % w/w % w/w % w/w % w/w % w/w % w/w Part A Biocide 0.001 0.01 0.1 5 0.001 0.01 0.1 5 1 PEI (I), (II), 0.001 0.001 0.001 0.001 2 2 2 2 1 (III), (IV) Part B Nansa SSA/A Dodecylbenzene Albright & 7.50 7.50 7.50 7.50 7.50 7.50 7.50 7.50 7.50 Sulfonic Wilson/ Acid Rhodia Edenor ® K12-18 Coconut Fatty BASF 12.50 12.50 12.50 12.50 12.50 12.50 12.50 12.50 12.50 Acid Neodol 23-6, C12-13 Pareth-7 Shell 10.00 10.00 10.00 10.00 10.00 10.00 10.00 10.00 10.00 5 E Chemicals Elfan NS 243 S Sodium Laureth Akzo 17.00 17.00 17.00 17.00 17.00 17.00 17.00 17.00 17.00 Sulfate 3 EO Nobel Part C 2-Propanol Isopropyl Merck 6.00 6.00 6.00 6.00 6.00 6.00 6.00 6.00 6.00 Alcohol Alcohol SD Alcohol 39-C Berkel 6.00 6.00 6.00 6.00 6.00 6.00 6.00 6.00 6.00 Trisodium Trisodium Fluka 5.50 5.50 5.50 5.50 5.50 5.50 5.50 5.50 5.50 Citrate Citrate Dihydrate Dihydrate Sodium Water (and) Fluka 10.70 10.70 10.70 10.70 10.70 10.70 10.70 10.70 10.70 Hydroxide Sodium (30% solution) Hydroxide Part D Water Aqua qs qs qs qs qs qs qs qs qs to 100 to 100 to 100 to 100 to 100 to 100 to 100 to 100 to 100

(X) Antibacterial Liquid Laundry Detergent

(10) TABLE-US-00016 INCI Name/ Chemical Trade name name Supplyer % w/w % w/w % w/w % w/w % w/w % w/w % w/w % w/w % w/w Part A Biocide 0.001 0.01 0.1 5 0.001 0.01 0.1 5 1 PEI (I), (II), 0.001 0.001 0.001 0.001 2 2 2 2 1 (III), (IV) Part B Marlon A 375 Sodium Sasol 8.7 8.7 8.7 8.7 8.7 8.7 8.7 8.7 8.7 Dodecylbenzene- sulfonate Marlipal O Trideceth-12 Sasol 3.50 3.50 3.50 3.50 3.50 3.50 3.50 3.50 3.50 13/129 Penta Sodium Penta Sodium Fluka 24.00 24.00 24.00 24.00 24.00 24.00 24.00 24.00 24.00 Triphosphate Triphosphate Sodium Sulfate Sodium Sulfate Merck 1.20 1.20 1.20 1.20 1.20 1.20 1.20 1.20 1.20 Sodium Water (and) Fluka 1.60 1.60 1.60 1.60 1.60 1.60 1.60 1.60 1.60 Hydroxide Sodium (10% solution) Hydroxide Glycerin Glycerin Merck 1.00 1.00 1.00 1.00 1.00 1.00 1.00 1.00 1.00 Oleic Acid Oleic Acid Fluka 0.90 0.90 0.90 0.90 0.90 0.90 0.90 0.90 0.90 Sodium Sodium Borate Fluka 1.80 1.80 1.80 1.80 1.80 1.80 1.80 1.80 1.80 Tetraborate Part C Tylose C 10000 Cellulose Gum Clariant 0.40 0.40 0.40 0.40 0.40 0.40 0.40 0.40 0.40 Part D Water Aqua qs qs qs qs qs qs qs qs qs to 100 to 100 to 100 to 100 to 100 to 100 to 100 to 100 to 100

(XI) Concentrated Anti-Bacterial Fabric Softener Based on Conventional Quat

(11) TABLE-US-00017 INCI Name/ Chemical Trade name name Supplyer % w/w % w/w % w/w % w/w % w/w % w/w % w/w % w/w % w/w Part A Biocide 0.001 0.01 0.1 5 0.001 0.01 0.1 5 1 PEI (I), (II), 0.001 0.001 0.001 0.001 2 2 2 2 1 (III), (IV) Part B Genapol O 100 Oleth-10 Clariant 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 Arquad 2HT-75 Quaternium 18 Akzo 15.00 15.00 15.00 15.00 15.00 15.00 15.00 15.00 15.00 (and) Nobel Isopropyl Alcohol Part C 2-Propanol Isopropyl Merck 3.00 3.00 3.00 3.00 3.00 3.00 3.00 3.00 3.00 Alcohol Magnesium Magnesium 1.00 1.00 1.00 1.00 1.00 1.00 1.00 1.00 1.00 Chloride Chloride saturated solution Part D Water Aqua qs qs qs qs qs qs qs qs qs to 100 to 100 to 100 to 100 to 100 to 100 to 100 to 100 to 100 Perfume and qs qs qs qs qs qs qs qs qs Preservative

(XII) Concentrated Anti-Bacterial Fabric Softener Based on Esterquat

(12) TABLE-US-00018 INCI Name/ Chemical Trade name name Supplyer % w/w % w/w % w/w % w/w % w/w % w/w % w/w % w/w % w/w Part A Biocide 0.001 0.01 0.1 5 0.001 0.01 0.1 5 1 PEI (I), (II), 0.001 0.001 0.001 0.001 2 2 2 2 1 (III), (IV) Part B Rewoquat WE 18 Dihydrogenated Evonik 15.00 15.00 15.00 15.00 15.00 15.00 15.00 15.00 15.00 Tallowethyl Hydroxyethyl- amonium Genapol O 100 Oleth-10 Clariant 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 Part C Antifoam DB 31 Dow 0.50 0.50 0.50 0.50 0.50 0.50 0.50 0.50 0.50 Corning 2-Propanol Isopropyl Merck 3.00 3.00 3.00 3.00 3.00 3.00 3.00 3.00 3.00 Alcohol Magnesium Magnesium 1.00 1.00 1.00 1.00 1.00 1.00 1.00 1.00 1.00 Chloride Chloride saturated solution Part D Water Aqua qs qs qs qs qs qs qs qs qs to 100 to 100 to 100 to 100 to 100 to 100 to 100 to 100 to 100 Perfume and qs qs qs qs qs qs qs qs qs Preservative

(XIII) Antibacterial Surface Disinfectant Spray, Providing Strong Immediate Bactericidal and Long Lasting Effects on Treated Surfaces

(13) TABLE-US-00019 INCI Name/ Chemical Trade name name Supplyer % w/w % w/w % w/w % w/w % w/w % w/w % w/w % w/w % w/w Part A Biocide 0.001 0.01 0.1 5 0.001 0.01 0.1 5 1 PEI (I), (II), (III), 0.001 0.001 0.001 0.001 2 2 2 2 1 (IV) Part B 2-Propanol Isopropyl Alcohol Merck 10.00 10.00 10.00 10.00 10.00 10.00 10.00 10.00 10.00 Texapon ® K12 Sodium Lauryl BASF 1.00 1.00 1.00 1.00 1.00 1.00 1.00 1.00 1.00 Sulfate Citric Acid Citric Acid Merck 3.00 3.00 3.00 3.00 3.00 3.00 3.00 3.00 3.00 Preservative qs qs qs qs qs qs qs qs qs Part C Sodium Water (and) Fluka ad pH ad pH ad pH ad pH ad pH ad pH ad pH ad pH ad pH Hydroxide Sodium Hydroxide 5.0 5.0 5.0 5.0 5.0 5.0 5.0 5.0 5.0 (10% solution) Part D Water Aqua qs to qs to qs to qs to qs to qs to qs to qs to qs to 100 100 100 100 100 100 100 100 100

(XIV) Surface Disinfectant Wet Wipes, Providing Strong Immediate Bactericidal and Long Lasting Effects on Treated Surfaces

(14) TABLE-US-00020 INCI Name/ Chemical Trade name name Supplyer % w/w % w/w % w/w % w/w % w/w % w/w % w/w % w/w % w/w Part A Biocide 0.001 0.01 0.1 5 0.001 0.01 0.1 5 1 PEI (I), (II), (III), 0.001 0.001 0.001 0.001 2 2 2 2 1 (IV) Part B Dowanol DPM PPG-2 Methyl Dow 10.00 10.00 10.00 10.00 10.00 10.00 10.00 10.00 10.00 Ether Chemical Texapon ® K12 Sodium Lauryl BASF 1.00 1.00 1.00 1.00 1.00 1.00 1.00 1.00 1.00 Sulfate Citric Acid Citric Acid Merck 3.00 3.00 3.00 3.00 3.00 3.00 3.00 3.00 3.00 Preservative qs qs qs qs qs qs qs qs qs Part C Sodium Hydroxide Water (and) Fluka ad pH ad pH ad pH ad pH ad pH ad pH ad pH ad pH ad pH (10% solution) Sodium Hydroxide 5.0 5.0 5.0 5.0 5.0 5.0 5.0 5.0 5.0 Water Aqua qs qs qs qs qs qs qs qs qs

(XV) Surface Disinfectant Spray, Providing Strong Immediate Bactericidal and Long Lasting Effects on Treated Surfaces

(15) TABLE-US-00021 INCI Name/ Chemical Trade name name Supplyer % w/w % w/w % w/w % w/w % w/w % w/w % w/w % w/w % w/w Part A Biocide 0.001 0.01 0.1 5 0.001 0.01 0.1 5 1 PEI (I), (II), (III), 0.001 0.001 0.001 0.001 2 2 2 2 1 (IV) Part B Glucopon ® 215 Alkyl Polyglycoside BASF 3.50 3.50 3.50 3.50 3.50 3.50 3.50 3.50 3.50 CS UP Dehydol ® 04 Deo Capryleth-4 BASF 1.70 1.70 1.70 1.70 1.70 1.70 1.70 1.70 1.70 Part C Perfume and qs qs qs qs qs qs qs qs qs Preservative Acetic Acid conc. Acetic Acid Fluka 2.40 2.40 2.40 2.40 2.40 2.40 2.40 2.40 2.40 Water Aqua qs to qs to qs to qs to qs to qs to qs to qs to qs to 100 100 100 100 100 100 100 100 100 Citric Acid Citric Acid Merck 9.00 9.00 9.00 9.00 9.00 9.00 9.00 9.00 9.00

(XVI) Surface Disinfectant Spray, Providing Strong Immediate Bactericidal and Long Lasting Effects on Treated Surfaces

(16) TABLE-US-00022 INCI Name/ Chemical Trade name name Supplyer % w/w % w/w % w/w % w/w % w/w % w/w % w/w % w/w % w/w Part A Biocide 0.001 0.01 0.1 5 0.001 0.01 0.1 5 1 PEI (I), (II), (III), 0.001 0.001 0.001 0.001 2 2 2 2 1 (IV) Part B Glucopon ® 215 Alkyl Polyglycoside BASF 3.50 3.50 3.50 3.50 3.50 3.50 3.50 3.50 3.50 CS UP Dehydol ® 04 Deo Capryleth-4 BASF 1.70 1.70 1.70 1.70 1.70 1.70 1.70 1.70 1.70 Sodium Cumene Sodium Cumene Hickson 5.00 5.00 5.00 5.00 5.00 5.00 5.00 5.00 5.00 Sulfonate Sulfonate Manro Part C Perfume and qs qs qs qs qs qs qs qs qs Preservative Citric Acid Citric Acid Merck ad pH ad pH ad pH ad pH ad pH ad pH ad pH ad pH ad pH 3.0 3.0 3.0 3.0 3.0 3.0 3.0 3.0 3.0 Water Aqua qs to qs to qs to qs to qs to qs to qs to qs to qs to 100 100 100 100 100 100 100 100 100

(XVII) Surface Disinfectant Spray, Providing Strong Immediate Bactericidal and Long Lasting Effects on Treated Surfaces

(17) TABLE-US-00023 INCI Name/ Chemical Trade name name Supplyer % w/w % w/w % w/w % w/w % w/w % w/w % w/w % w/w % w/w Part A Biocide 0.001 0.01 0.1 5 0.001 0.01 0.1 5 1 PEI (I), (II), (III), 0.001 0.001 0.001 0.001 2 2 2 2 1 (IV) Part B Texapon ® K12 Sodium Lauryl BASF 1.00 1.00 1.00 1.00 1.00 1.00 1.00 1.00 1.00 Sulfate Citric Acid Citric Acid Merck 3.00 3.00 3.00 3.00 3.00 3.00 3.00 3.00 3.00 Preservative qs qs qs qs qs qs qs qs qs Dowanol DPM PPG-2 Methyl Dow 10.00 10.00 10.00 10.00 10.00 10.00 10.00 10.00 10.00 Ether Chemical Part C Sodium Hydroxide Water (and) Fluka ad pH ad pH ad pH ad pH ad pH ad pH ad pH ad pH ad pH (10% solution) Sodium Hydroxide 5.0 5.0 5.0 5.0 5.0 5.0 5.0 5.0 5.0

(XVIII) Surface Disinfectant Spray, Providing Strong Immediate Bactericidal and Long Lasting Effects on Treated Surfaces. Formulation can be Diluted: 1 Part to 9 Parts of Water

(18) TABLE-US-00024 INCI Name/ Chemical Trade name name Supplyer % w/w % w/w % w/w % w/w % w/w % w/w % w/w % w/w % w/w Part A Biocide 0.001 0.01 0.1 5 0.001 0.01 0.1 5 1 PEI (I), (II), (III), (IV) 0.001 0.001 0.001 0.001 2 2 2 2 1 Part B Texapon ® K12 Sodium Lauryl BASF 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 Sulfate 1-Propanol Propyl Alcohol Merck 10.00 10.00 10.00 10.00 10.00 10.00 10.00 10.00 10.00 Part C Hydrogen Peroxide Hydrogen Merck 10.00 10.00 10.00 10.00 10.00 10.00 10.00 10.00 10.00 30% Peroxide Part D Citric Acid sol. 20% Citric Acid Merck ad pH ad pH ad pH ad pH ad pH ad pH ad pH ad pH ad pH 3.0 3.0 3.0 3.0 3.0 3.0 3.0 3.0 3.0 Preservative qs qs qs qs qs qs qs qs qs Part E Water Aqua qs to qs to qs to qs to qs to qs to qs to qs to qs to 100 100 100 100 100 100 100 100 100

(XIX) Surface Disinfectant Spray, Providing Immediate Bactericidal and Long Lasting Effects on Treated Surfaces

(19) TABLE-US-00025 INCI Name/ Chemical Trade name name Supplyer % w/w % w/w % w/w % w/w % w/w % w/w % w/w % w/w % w/w Part A Biocide 0.001 0.01 0.1 5 0.001 0.01 0.1 5 1 PEI (I), (II), (III), 0.001 0.001 0.001 0.001 2 2 2 2 1 (IV) Part B 2-Propanol Isopropyl Alcohol Merck 10.00 10.00 10.00 10.00 10.00 10.00 10.00 10.00 10.00 Glucopon ® 215 Alkyl Polyglycoside BASF 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 CS UP Part C Perfume and qs qs qs qs qs qs qs qs qs Preservative Water Aqua qs to qs to qs to qs to qs to qs to qs to qs to qs to 100 100 100 100 100 100 100 100 100 Citric Acid Citric Acid Merck 1.00 1.00 1.00 1.00 1.00 1.00 1.00 1.00 1.00 Sodium Hydroxide Water (and) Fluka ad pH ad pH ad pH ad pH ad pH ad pH ad pH ad pH ad pH (10% solution) Sodium Hydroxide 5.0 5.0 5.0 5.0 5.0 5.0 5.0 5.0 5.0

(XX) Surface Disinfectant Wet Wipes, Providing Immediate Bactericidal and Long Lasting Effects on Treated Surfaces

(20) TABLE-US-00026 INCI Name/ Chemical Trade name name Supplyer % w/w % w/w % w/w % w/w % w/w % w/w % w/w % w/w % w/w Part A Biocide 0.001 0.01 0.1 5 0.001 0.01 0.1 5 1 PEI (I), (II), (III), 0.001 0.001 0.001 0.001 2 2 2 2 1 (IV) Part B 2-Propanol Isopropyl Alcohol Merck 10.00 10.00 10.00 10.00 10.00 10.00 10.00 10.00 10.00 Glucopon ® 215 Alkyl Polyglycoside BASF 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 CS UP Part C Perfume and qs qs qs qs qs qs qs qs qs Preservative Water Aqua qs to qs to qs to qs to qs to qs to qs to qs to qs to 100 100 100 100 100 100 100 100 100 Citric Acid Citric Acid Merck 1.00 1.00 1.00 1.00 1.00 1.00 1.00 1.00 1.00 Sodium Hydroxide Water (and) Fluka ad pH ad pH ad pH ad pH ad pH ad pH ad pH ad pH ad pH (10% solution) Sodium Hydroxide 5.0 5.0 5.0 5.0 5.0 5.0 5.0 5.0 5.0

(XXI) Antibacterial Window Cleaner, Providing Immediate Bactericidal and Long Lasting Effects on Treated Surfaces

(21) TABLE-US-00027 INCI Name/ Chemical Trade name name Supplyer % w/w % w/w % w/w % w/w % w/w % w/w % w/w % w/w % w/w Part A Biocide 0.001 0.01 0.1 5 0.001 0.01 0.1 5 1 PEI (I), (II), (III), 0.001 0.001 0.001 0.001 2 2 2 2 1 (IV) Part B 2-Propanol Isopropyl Alcohol Merck 10.00 10.00 10.00 10.00 10.00 10.00 10.00 10.00 10.00 Glucopon ® 215 Alkyl Polyglycoside BASF 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 CS UP Part C Perfume and qs qs qs qs qs qs qs qs qs Preservative Water Aqua qs to qs to qs to qs to qs to qs to qs to qs to qs to 100 100 100 100 100 100 100 100 100 Citric Acid Citric Acid Merck 1.00 1.00 1.00 1.00 1.00 1.00 1.00 1.00 1.00 Sodium Hydroxide Water (and) Fluka ad pH ad pH ad pH ad pH ad pH ad pH ad pH ad pH ad pH (10% solution) Sodium Hydroxide 5.0 5.0 5.0 5.0 5.0 5.0 5.0 5.0 5.0

(XXII) Bactericidal Dishwashing Liquid, Providing Strong Bactericidal and Long Lasting Antibacterial Protection of Dishwashing Utensils

(22) TABLE-US-00028 INCI Name/ Chemical Trade name name Supplyer % w/w % w/w % w/w % w/w % w/w % w/w % w/w % w/w % w/w Part A Biocide 0.001 0.01 0.1 5 0.001 0.01 0.1 5 1 PEI (I), (II), (III), 0.001 0.001 0.001 0.001 2 2 2 2 1 (IV) Part B Marlon A 375 Sodium Sasol 7.00 7.00 7.00 7.00 7.00 7.00 7.00 7.00 7.00 Dodecylbenzene- sulfonate Sodium Cumene Sodium Cumene Hickson 5.00 5.00 5.00 5.00 5.00 5.00 5.00 5.00 5.00 Sulfonate Sulfonate Manro Part C Citric Acid Citric Acid Merck 3.00 3.00 3.00 3.00 3.00 3.00 3.00 3.00 3.00 Benzoic Acid Benzoic Acid Merck 0.50 0.50 0.50 0.50 0.50 0.50 0.50 0.50 0.50 Sodium Chloride Sodium Chloride Fluka 1.00 1.00 1.00 1.00 1.00 1.00 1.00 1.00 1.00 Sodium Sulfate Sodium Sulfate Merck 3.50 3.50 3.50 3.50 3.50 3.50 3.50 3.50 3.50 Sodium Hydroxide Water (and) Fluka ad pH ad pH ad pH ad pH ad pH ad pH ad pH ad pH ad pH (30% solution) Sodium Hyroxide 5.0 5.0 5.0 5.0 5.0 5.0 5.0 5.0 5.0 Part D Water Aqua qs qs qs qs qs qs qs qs qs

(XVII) High Foaming and Mild Dishwashing Liquid, Providing Strong Bactericidal and Long Lasting Antibacterial Protection of Dishwashing Utensils and Sponges

(23) TABLE-US-00029 INCI Name/ Chemical Trade name name Supplyer % w/w % w/w % w/w % w/w % w/w % w/w % w/w % w/w % w/w Part A Biocide 0.001 0.01 0.1 5 0.001 0.01 0.1 5 1 PEI (I), (II), (III), 0.001 0.001 0.001 0.001 2 2 2 2 1 (IV) Part B Texapon ® LS 35 Lauryl-myristyl BASF 7.00 7.00 7.00 7.00 7.00 7.00 7.00 7.00 7.00 sulfate Na-salt Texapon ® K14 Sodium Myreth BASF 7.00 7.00 7.00 7.00 7.00 7.00 7.00 7.00 7.00 Special Sulfate Plantacare ® 1200 Lauryl Glucoside BASF 4.00 4.00 4.00 4.00 4.00 4.00 4.00 4.00 4.00 UP Dehyton ® AB 30 Coco Betaine BASF 1.1 1.1 1.1 1.1 1.1 1.1 1.1 1.1 1.1 Alcohol SD Alcohol 39-C Berkel 5.00 5.00 5.00 5.00 5.00 5.00 5.00 5.00 5.00 Part C Sodium Chloride Sodium Chloride Fluka 1.00 1.00 1.00 1.00 1.00 1.00 1.00 1.00 1.00 Part D Citric Acid Citric Acid Merck ad pH ad pH ad pH ad pH ad pH ad pH ad pH ad pH ad pH 6.5 6.5 6.5 6.5 6.5 6.5 6.5 6.5 6.5 Preservative qs qs qs qs qs qs qs qs qs Water Aqua qs qs qs qs qs qs qs qs qs

(XXIII) Disinfectant Toilet Cleaner, Providing Strong Immediate Bactericidal and Long Lasting Effects on Treated Surfaces

(24) TABLE-US-00030 INCI Name/ Chemical Trade name name Supplyer % w/w % w/w % w/w % w/w % w/w % w/w % w/w % w/w % w/w Part A Biocide 0.001 0.01 0.1 5 0.001 0.01 0.1 5 1 PEI (I), (II), (III), 0.001 0.001 0.001 0.001 2 2 2 2 1 (IV) Glucopon ® 215 Alkyl Polyglycoside BASF 3.50 3.50 3.50 3.50 3.50 3.50 3.50 3.50 3.50 CS UP Neodol 91-8E C9-11 pareth-8 Caldic 1.70 1.70 1.70 1.70 1.70 1.70 1.70 1.70 1.70 Part B RHEOVIS ® CSP Cationic Polymer BASF 1.30 1.30 1.30 1.30 1.30 1.30 1.30 1.30 1.30 Part C Acetic Acid conc. Acetic Acid Fluka 2.40 2.40 2.40 2.40 2.40 2.40 2.40 2.40 2.40 Citric Acid Citric Acid Merck 9.00 9.00 9.00 9.00 9.00 9.00 9.00 9.00 9.00 Part D IRAGON ®Blue Acid blue 80 BASF 0.50 0.50 0.50 0.50 0.50 0.50 0.50 0.50 0.50 ABL80 sol. 1% Water Aqua qs to qs to qs to qs to qs to qs to qs to qs to qs to 100 100 100 100 100 100 100 100 100

(XIV) Shoe Deodorant (Alcohol Based Spray Formulation)

(25) TABLE-US-00031 INCI Name/ Chemical Trade name name Supplyer % w/w % w/w % w/w % w/w % w/w % w/w % w/w % w/w % w/w Part A Biocide 0.001 0.01 0.1 5 0.001 0.01 0.1 5 1 PEI (I), (II), (III) 0.001 0.001 0.001 0.001 2 2 2 2 1 (IV) Part B Ethanol absolut. Alcohol Riedel 20.00 20.00 20.00 20.00 20.00 20.00 20.00 20.00 20.00 Propylene Glycol Propylene BASF 20.00 20.00 20.00 20.00 20.00 20.00 20.00 20.00 20.00 Glycol Marlipal 24/99 Laureth-9 Sasol 1.00 1.00 1.00 1.00 1.00 1.00 1.00 1.00 1.00 Glycerin Glycerin Merck 1.00 1.00 1.00 1.00 1.00 1.00 1.00 1.00 1.00 Perfume qs qs qs qs qs qs qs qs qs Part C Water Aqua qs to 100 qs to 100 qs to 100 qs to 100 qs to qs to qs to qs to qs to 100 100 100 100 100 Biocides used in each of the above formulations (I) to (XXIV) are: Bronopol, Phenoxyethanol, Glutaraldehyde, Formic Acid, Glyoxal, 2,4-Dichlorobenzylalcohol, Dazomet (3,5-Dimetyl-1,3-5-thiadiazinane-2-thione), 1,3,5-Tris-(2-hydroxyethyl)-1,3,5-hexahydrotriaxine, Cybutryne (2-Methylthio-4-tert-butylamino cyclopropylamino-6-(1,3,5-triazine)), Irgaguard ® B5000, Irgaguard ® B6000, Irgaguard ® B7000, Triclosan, 4,4′-Dichloro 2′-hydroxydiphenylether (Diclosan, a.i. of Tinosan ®HP 100).

(26) The following examples illustrate the invention. Wherever noted, room temperature (r.t.) depicts a temperature from the range 22-25° C.; over night means a period of 12 to 15 hours; percentages are given by weight, if not indicated otherwise.

ABBREVIATIONS

(27) HPLC high pressure liquid chromatography

(28) Mw molecular weight (usually as detected by GPC)

(29) GPC gel permeation chromatography

(30) DSC differential scanning calorimetry

(31) NMR nuclear magnetic resonance

(32) a.i. active ingredient

Example 1

(33) Microbiological data demonstrating synergistic efficacy of biocides (reduction of the number of viable cells expressed as common logarythm (log redn.)) in combinations with Polyethylenimines are summarized in the following tables. Lines in grey shading indicate control sample (no PEI or no biocide added):

(34) TABLE-US-00032 TABLE 1 Examples for synergistic bactericidal effects of biocides in combination with Polyethylenimines. The bactericidal activities are determined according to to method EN1040 against Pseudomonas aeruginosa ATCC 15442. log redn. Conc. of PEI Conc. of Biocide after Contact time [ppm [% 24 PEI a.i.] Biocide a.i.] 1 hr 3 hrs 6 hrs hrs Lupasol ® 100 2.3 3.2 4.1 >6 FG Phenoxyethanol 1 <1 1.3 2.8 >6 Lupasol ® 100 Phenoxyethanol 1 >5 >5 >5 >5 FG PEI (II) 50 3.5 4.4 >5 >6 Phenoxyethanol 1 <1 1.3 2.8 >6 PEI (II) 50 Phenoxyethanol 1 >5 >5 >5 >5 Lupasol ® 50 >5 5.0 5 >5 WF Phenoxyethanol 1 <1 1.3 2.8 >6 Lupasol ® 50 Phenoxyethanol 1 >5 5.0 5.0 >5 WF PEI (IV) 80 <1 <1 <1 >1 Phenoxyethanol 1 <1 1.3 2.8 >6 PEI (IV) 80 Phenoxyethanol 1 >5 >5 >5 >5 PEI (IV) 80 <1 <1 <1 >6 Bronopol 0.1 <1 <1 4.2 2.4 PEI (IV) 80 Bronopol 0.1 <1 1.7 >5 >5

(35) TABLE-US-00033 TABLE 2 Examples for synergistic bactericidal effects of biocides in combination with Polyethylenimines. The bactericidal activities are determined according to to method EN1040 against Staphylococcus aureus ATCC 6538. Conc. of PEI Conc. of Biocide log redn. after Contact time [ppm [% 24 PEI a.i.] Biocide a.i.] 1 hr 3 hrs 6 hrs hrs Lupasol ® 100 <1 1.2 1.2 2.3 FG Phenoxyethanol 1 <1 <1 <1 1.1 Lupasol ® 100 Phenoxyethanol 1 1.0 1.7 4.5 >5 FG PEI (II) 50 1.3 2.1 1.9 4.4 Phenoxyethanol 1 <1 <1 <1 1.1 PEI (II) 50 Phenoxyethanol 1 1.3 4.4 >5 >5 Lupasol ® 50 <1 2.1 3.1 >5 WF Phenoxyethanol 1 <1 <1 <1 1.1 Lupasol ® 50 Phenoxyethanol 1 >5 >5 >5 >5 WF PEI (IV) 50 <1 <1 <1 <1 Phenoxyethanol 1 <1 <1 <1 1.1 PEI (IV) 50 Phenoxyethanol 1 <1 <1 <1 4.2 PEI (IV) 80 <1 <1 <1 >1 Bronopol 0.1 <1 <1 <1 2.4 PEI (IV) 80 Bronopol 0.1 <1 <1 <1 >5

(36) TABLE-US-00034 TABLE 3 Boosting efficacy of Polyethylenimines and Tinosan ® HP 100. The bactericidal activities are determined according to to method EN1276 against Staphylococcus aureus ATCC 6538. Conc. of PEI Conc. of Biocide log redn. after [ppm [ppm Contact time PEI a.i.] Biocide a.i.] 5 min Tinosan ® HP 100 250 4.9 Lupasol ® FG 100 Tinosan ® HP 100 250 5.1 Lupasol ® FG 1000 Tinosan ® HP 100 250 5.4

(37) An independent study (control) shows that 1000 ppm (a.i.) Lupasol® FG alone has no bactericidal activity at a contact time of 5 minutes (<1 log reduction) against S. aureus ATCC 6538.

Example 2

(38) Preservation Challenge Test according to the test method for topical products described in Eur. Pharm. edition 7.1 against several typical bacterial or fungal spoilage microorganisms. A surfactant formulation (45% Cocoamidopropyl betaine in water) is preserved with 0.5% phenoxyethanol alone or in combination with 0.1% polyethyleneimine (referring to active ingredient).

(39) Samples are inoculated with 1.5 to 3.5×10.sup.5 cfu/ml as indicated in Table 5 below. After 7 days of incubation at 20° C., samples are taken and the total viable count is determined. Log reductions are calculated with reference to the initial germ load. The results show that the combination of phenoxyethanol with polyethylenimines perform better with regard to antimicrobial activity than phenoxyethanol alone. Table 4 shows the composition of formulations, Table 5 shows antimicrobial efficacies (log red.) after 7d of contact time.

(40) TABLE-US-00035 TABLE 4 Formulations Sample 25 Sample 26 Sample 27 Sample 28 Ingredient (comparison) (invention) (invention) (invention) surfactant 11.11% 11.11% 11.11% 11.11% formulation phenoxyethanol 0.5% 0.5% 0.5% 0.5% Lupasol ® WF — 0.1% — — Lupasol ® FG — — 0.1% — PEI (IV) — — — 0.1% Water to 100% to 100% to 100% to 100% pH is adjusted to 7 by addition of citric acid and/or NaOH.

(41) TABLE-US-00036 TABLE 5 Antimicrobial effect (log red.) after 7 days of contact time Sam- Sam- Sam- Initial Sam- ple 26 ple 27 ple 28 germ load ple 25 (inven- (inven- (inven- Test organism [cfu/ml] (comp.) tion) tion) tion) P. aeruginosa 2.5 × 10.sup.5 ≤1 ≥3 ≥3 ≤1 ATCC 9027 S. aureus 1.9 × 10.sup.5 ≥3 5 5 ≥3 ATCC 6538 C. albicans 3.4 × 10.sup.5 ≥4 6 6 6 ATCC 10231

Example 3

Bactericidal Effect

(42) Bactericidal activities are determined according to method EN1040 against Staphylococcus aureus ATCC 6538, Pseudomonas aeruginosa ATCC 15442 and Escherichia coli ATCC 10536. Suspensions adjusted to pH 4, pH 7 and pH 9 by addition of citric acid and/or NaOH are used. Bactericidal efficacies are given in the following tables as log reduction calculated from total viable counts compared to the water control. The microbicidal activities are determined after the following contact times: 5 minutes, 15 minutes, 30 minutes, 60 minutes, 3 hours, 6 hours and 24 hours. Effects (log red.) by bactericide alone, polyethylenimine alone, and by the combination of bactericide and polyethylenimine are compiled in Tables 6 (Lupasol® FG), Table 7 (Lupasol® WF) and Table 8 (PEI (IV)).

(43) TABLE-US-00037 TABLE 6 Bactericidal effect on Staphylococcus aureus (S), Pseudomonas aeruginosa (P) and Escherichia coli (E) by combination of biocide with Lupasol ® FG log redn. at Polymer log redn. Active Concentration Active Biocide Lupasol ® FG Conc. alone [1000 [100 [10 Biocide [ppm] (comparison) ppm] ppm] ppm] 5-chloro-2-(4- 10000 chlorophenoxy) 1000 phenol 100 10 1 0.1 2,4,4′- 10000 trichloro-2′ 1000 hydroxyl- 100 diphenylether 10 1 0.1 1,5- 10000 pentanedial 5000 2500 500 50 5 1,2-ethanedial 200000 100000 20000 4000 2000 400 formic acid 20000 16000 8000 4000 1000 500 2-bromo-2- 10000 nitropropane- 5000 1,3-diol 1000 500 100 50 2-phenoxy-1- 10000 ethanol 5000 1000 500 100 50 Polymer (comp.)

(44) TABLE-US-00038 TABLE 7 Bactericidal effect (log redn.) on Staphylococcus aureus (S), Pseudomonas aeruginosa (P) and Escherichia coli (E) by combination of biocide with Lupasol ® WF Polymer Active Concentration Active Biocide Lupasol ® WF Conc. alone [1000 [100 [10 Biocide [ppm] (comparison) ppm] ppm] ppm] 5-chloro-2-(4- 10000 chlorophenoxy) 1000 phenol 100 10 1 0.1 2,4,4′- 10000 trichloro-2′ 1000 hydroxyl- 100 diphenylether 10 1 0.1 1,5- 10000 pentanedial 5000 2500 500 50 5 1,2-ethanedial 200000 100000 20000 4000 2000 400 formic acid 20000 16000 8000 4000 1000 500 2-bromo-2- 10000 nitropropane- 5000 1,3-diol 1000 500 100 50 2-phenoxy-1- 10000 ethanol 5000 1000 500 100 50 Polymer alone (comparison)

(45) TABLE-US-00039 TABLE 8 Bactericidal effect (log redn.) on Staphylococcus aureus (S), Pseudomonas aeruginosa (P) and Escherichia coli (E) by combination of biocide with PEI (IV) Polymer Active Concentration Active Biocide PEI (IV) Conc. alone [1000 [100 [10 Biocide [ppm] (comparison) ppm] ppm] ppm] 5-chloro-2-(4- 10000 chlorophenoxy) 1000 phenol 100 10 1 0.1 2,4,4′- 10000 trichloro-2′ 1000 hydroxyl- 100 diphenylether 10 1 0.1 1,5- 10000 pentanedial 5000 2500 500 50 5 1,2-ethanedial 200000 100000 20000 4000 2000 400 formic acid 20000 16000 8000 4000 1000 500 2-bromo-2- 10000 nitropropane- 5000 1,3-diol 1000 500 100 50 2-phenoxy-1- 10000 ethanol 5000 1000 500 100 50 Polymer alone (comparison)

Example 4

Fungicidal Effect

(46) Fungicidal activities are determined according to method EN1275 against Candida albicans ATCC 10231 and Aspergillus brasiliensis ATCC 16404. Suspensions adjusted to pH 4, pH 7 and pH 9 by addition of citric acid and/or NaOH are used. Efficacies are given as log reduction calculated from total viable counts compared to the water control. The microbicidal activities are determined after the following contact times: 5 minutes, 15 minutes, 30 minutes, 60 minutes, 3 hours, 6 hours and 24 hours. Fungicidal effect (log red.) by the biocide alone, polyethylenimine alone, and by the combination of biocide and polyethylenimine are compiled in Tables 9 (Lupasol® FG), Table 10 (Lupasol® WF) and Table 11 (PEI (IV)).

(47) TABLE-US-00040 TABLE 9 Fungicidal effect (log redn.) on Candida albicans (C) and Aspergillus brasiliensis (A) by combination of biocide with Lupasol ® FG Polymer Active Concentration Active Biocide Lupasol ® FG Conc. alone [1000 [100 [10 Biocide [ppm] (comparison) ppm] ppm] ppm] 5-chloro-2-(4- 10000 chlorophenoxy) 1000 phenol 100 10 1 0.1 2,4,4′- 10000 trichloro-2′ 1000 hydroxyl- 100 diphenylether 10 1 0.1 1,5- 10000 pentanedial 5000 2500 500 50 5 1,2-ethanedial 200000 100000 20000 4000 2000 400 formic acid 20000 16000 8000 4000 1000 500 2-bromo-2- 10000 nitropropane- 5000 1,3-diol 1000 500 100 50 2-phenoxy-1- 10000 ethanol 5000 1000 500 100 50 Polymer alone (comparison)

(48) TABLE-US-00041 TABLE 10 Fungicidal effect (log redn.) on Candida albicans (C) and Aspergillus brasiliensis (A) by combination of biocide with Lupasol ® WF Polymer Active Concentration Active Biocide Lupasol ® WF Conc. alone [1000 [100 [10 Biocide [ppm] (comparison) ppm] ppm] ppm] 5-chloro-2-(4- 10000 chlorophenoxy) 1000 phenol 100 10 1 0.1 2,4,4′- 10000 trichloro-2′ 1000 hydroxyl- 100 diphenylether 10 1 0.1 1,5- 10000 pentanedial 5000 2500 500 50 5 1,2-ethanedial 200000 100000 20000 4000 2000 400 formic acid 20000 16000 8000 4000 1000 500 2-bromo-2- 10000 nitropropane- 5000 1,3-diol 1000 500 100 50 2-phenoxy-1- 10000 ethanol 5000 1000 500 100 50 Polymer alone (comparison)

(49) TABLE-US-00042 TABLE 8 Fungicidal effect (log redn.) on Candida albicans (C) and Aspergillus brasiliensis (A) by combination of biocide with PEI (IV) Polymer Active Concentration Active Biocide PEI (IV) Conc. alone [1000 [100 [10 Biocide [ppm] (comparison) ppm] ppm] ppm] 5-chloro-2-(4- 10000 chlorophenoxy) 1000 phenol 100 10 1 0.1 2,4,4′- 10000 trichloro-2′ 1000 hydroxyl- 100 diphenylether 10 1 0.1 1,5- 10000 pentanedial 5000 2500 500 50 5 1,2-ethanedial 200000 100000 20000 4000 2000 400 formic acid 20000 16000 8000 4000 1000 500 2-bromo-2- 10000 nitropropane- 5000 1,3-diol 1000 500 100 50 2-phenoxy-1- 10000 ethanol 5000 1000 500 100 50 Polymer alone (comparison)