Enhancing the antimicrobial activity of biocides with polymers

Abstract

Disclosed are compositions comprising a) an antimicrobial agent, which is selected from the group consisting of biocides containing halogen atoms and/or containing phenolic moieties, formic acid, chlorine dioxide, chlorine dioxide generating compounds, dialdehydes; components containing an antimicrobial metal such as antimicrobial silver, and b) a polyamine, especially a polyethylenimine. The polyamine is effective as a booster for the antimicrobial agent.

Claims

1. A liquid composition comprising: a) phenoxyethanol, and b) a polyethylenimine, wherein the polyethylenimine is an oligomer or polymer grafted with 0.01 to 100 parts by weight of ethylene oxide on 1 part by weight of the polyethylenimine; wherein the polyethylenimine and the phenoxyethanol form a synergistic mixture in which the polyethylenimine enhances antimicrobial activity of the phenoxyethanol against at least one of Pseudomonas aeruginosa and Staphylococcus aureus; and wherein one of conditions (I) or (II) also apply to the liquid composition: (I) the phenoxyethanol is present in an amount from 0.1% to 1% b.w. relative to the liquid composition total weight, and the polyethylenimine is present in an amount from 0.005% to 1% b.w. relative to the liquid composition total weight; or (II) the phenoxyethanol is present in an amount from 0.1% to 5% b.w. relative to the liquid composition total weight, and the polyethylenimine is present in an amount from 0.001 to 10 parts by weight per 1 part by weight of the phenoxyethanol, the liquid composition further comprises a surfactant, and the liquid composition is in the form of an aqueous composition further comprising water.

2. The liquid composition of claim 1, wherein: condition (I) applies to the liquid composition; and the polyethylenimine is present in an amount from 0.001 to 2 parts by weight per 1 part by weight of the phenoxyethanol.

3. The liquid composition of claim 1, wherein condition (II) applies to the liquid composition.

4. The liquid composition of claim 3, wherein the water is present in the aqueous composition in an amount of at least 50% b.w. relative to the liquid composition total weight.

5. The liquid composition of claim 1, wherein: the phenoxyethanol is present in an amount from 0.1% to 1% b.w. relative to the liquid composition total weight; and the polyethylenimine is present in an amount from 50 ppm to 1000 ppm relative to the liquid composition total weight.

6. The liquid composition of claim 1, wherein the liquid composition is in the form of a liquid home care formulation or a liquid personal care formulation.

7. The liquid composition of claim 1, wherein the polyethylenimine has a number-average molecular weight in the range from 500 to 2,000,000 g/mol.

8. The liquid composition of claim 1, wherein the polyethylenimine is a grafted variant of a branched polyethylenimine homopolymer, whose uncharged form conforms to the empirical formula —(CH.sub.2CH.sub.2NH).sub.n-, wherein n ranges from approximately 10 to 100000.

9. The liquid composition of claim 1, further comprising: at least one of a surface active agent, a hydrotropic agent, a further antimicrobial agent, a stabilizing agent for phenoxyethanol in the composition, and a stabilizing agent for polyamine in the composition.

10. The liquid composition of claim 1, further comprising a surfactant selected from the group consisting of anionic surfactants, cationic surfactants, nonionic surfactants, amphoteric surfactants, and combinations thereof.

11. The liquid composition of claim 1, further comprising a surfactant in an amount from 0.001% to 80% b.w. relative to the liquid composition total weight.

12. The liquid composition of claim 1, wherein the liquid composition is in the form of a liquid home care formulation selected from the group consisting of a disinfectant, all purpose cleaner, laundry detergent, dishwashing liquid, deodorant, fabric conditioner, product for disinfection and sanitization of hard surfaces, floor cleaner, glass cleaner, kitchen cleaner, bath cleaner, sanitary cleaner, hygiene rinse product for fabrics, carpet cleaner, furniture cleaner, or a product for conditioning, sealing, caring or treating hard and soft surfaces.

13. The liquid composition of claim 1, further comprising at least one further component selected from the group consisting of alkoxylated alcohols, sodium C14-17alkyl-sec-sulfonate, lauryl-myristyl sulfate sodium salt, diethyleneglycol monobutyl ether, citric acid, acid blue, a mixture of sodium benzotriazolyl butylphenol sulfonate and buteth-3 and tributylcitrate, hydroxydichlorodiphenyl ether, alkyl polyglycoside, sodium cumenesulfonate, sodium C13-17-alkanesulfonate, trisodiumcitrate dihydrate, isopropyl alcohol, C12-15 pareth-7, C14-15 pareth-7, quaternium 18, dihydrogenated tallowethyl hydroxyethylamonium, sodium dodecylbenzenesulfonate, dodecylbenzenesulfonic acid, a mixture of sodium tallowate and sodium cocoate, triethanolamine, fluorescent whitening agent, trideceth-12, pentasodium triphosphate, sodium sulfate, a mixture of water and sodium hydroxide, glycerin, oleic acid, sodium borate, cellulose gum, oleth-10, magnesium chloride, PPG-2 methylether, sodium lauryl sulfate, capryleth-4, acetic acid, propyl alcohol, hydrogen peroxide, benzoic acid, sodium chloride, sodium sulfate, lauryl glucoside, coco betaine, SD Alcohol 39-C, C9-11 pareth-8, a further cationic polymer, propylene glycol, laureth-9, and combinations thereof.

14. The liquid composition of claim 1, wherein: condition (II) applies to the liquid composition; the phenoxyethanol is present in an amount from 0.1% to 1% b.w. relative to the liquid composition total weight; the polyethylenimine is present in an amount from 0.005% to 1% b.w. relative to the liquid composition total weight; and the polyethylenimine is present in an amount from 0.001 to 1 parts by weight per 1 part by weight of the phenoxyethanol.

15. A method of manufacturing a liquid formulation selected from the group consisting of home care formulations and personal care formulations, which method comprises: adding the liquid composition of claim 1 to the liquid formulation.

16. The liquid composition of claim 1, wherein: condition (II) applies to the liquid composition; the phenoxyethanol is present in an amount from 0.1% to 2% b.w. relative to the liquid composition total weight; the polyethylenimine is present in an amount from 0.005% to 1% b.w. relative to the liquid composition total weight; and the polyethylenimine is present in an amount from 0.001 to 2 parts by weight per 1 part by weight of the phenoxyethanol.

17. The liquid composition of claim 14, wherein: the polyethylenimine is present in an amount from 0.005 to 1 parts by weight per 1 part by weight of the phenoxyethanol.

Description

FORMULATION EXAMPLES

(1) (I) All Purpose Cleaner Providing Antibacterial Protection

(2) TABLE-US-00007 INCI Name/ Trade name Chemical name Supplyer % w/w % w/w % w/w % w/w % w/w % w/w % w/w % w/w % w/w Part Biocide 0.001-5% 0.001 0.01 0.1 5 0.001 0.01 0.1 5 1 A PEI (I), (II), (III), 0.001 0.001 0.001 0.001 2 2 2 2 1 (IV) Part Plurafac LF 400 Alkoxylated BASF 10.00 10.00 10.00 10.00 10.00 10.00 10.00 10.00 10.00 B Alcohol Hostapur SAS 60 Sodium C14-17 Clariant 14.00 14.00 14.00 14.00 14.00 14.00 14.00 14.00 14.00 Alkyl Sec Sulfonate Texapon .sup.® LS 35 Lauryl-myristyl BASF 5.00 5.00 5.00 5.00 5.00 5.00 5.00 5.00 5.00 sulfate Na-salt Diethylene Glycol Diethylene Merck 5.00 5.00 5.00 5.00 5.00 5.00 5.00 5.00 5.00 Monobutyl Ether Glycol Monobutyl Ether Part Perfume and qs qs qs qs qs qs qs qs qs C Preservative Citric Acid Citric Acid Merck ad pH ad pH ad pH ad pH ad pH ad pH ad pH ad pH ad pH 6.5 6.5 6.5 6.5 6.5 6.5 6.5 6.5 6.5 IRAGON .sup.® Blue Acid Blue BASF 0.01 0.01 0.01 0.01 0.01 0.01 0.01 0.01 0.01 ABL 9 sol. 1% Cibafast .sup.® H Sodium Ben- BASF 0.05 0.05 0.05 0.05 0.05 0.05 0.05 0.05 0.05 Liquid zotriazolyl Bu- tylphenol Sul- fonate (and) Buteth-3 (and) Tributyl Citrate Part Water Aqua qs to qs qs qs qs qs qs qs qs D 100 % w/w: wt % as supplied

(3) (II) All Purpose Cleaner, Providing Strong Immediate Bactericidal and Long Lasting Effects on Treated Surface

(4) TABLE-US-00008 INCI Name/ Chemical Sup- Trade name name plyer % w/w % w/w % w/w % w/w % w/w % w/w % w/w % w/w % w/w Part Biocide 0.001 0.01 0.1 5 0.001 0.01 0.1 5 1 A PEI (I), (II), 0.001 0.001 0.001 0.001 2 2 2 2 1 (III), (IV) Part Tinosan .sup.® Hydroxydichlorodi- BASF 0.30 0.30 0.30 0.30 0.30 0.30 0.30 0.30 0.30 B HP 100 phenyl Ether Part Texapon .sup.® Lauryl-myristyl BASF 10.00 10.00 10.00 10.00 10.00 10.00 10.00 10.00 10.00 C LS 35 sulfate Na-salt Diethylene Diethylene Glycol Merck 5.00 5.00 5.00 5.00 5.00 5.00 5.00 5.00 5.00 Glycol Monobutyl Ether Monobutyl Ether Glucopon .sup.® Alkyl BASF 3.00 3.00 3.00 3.00 3.00 3.00 3.00 3.00 3.00 215 CS Polyglycoside UP Part Perfume qs qs qs qs qs qs qs qs qs D and Preservative Citric Acid Citric Acid Merck ad pH ad pH ad pH ad pH ad pH ad pH ad pH ad pH ad pH 3.0 3.0 3.0 3.0 3.0 3.0 3.0 3.0 3.0 Water Aqua qs to qs to qs to qs to qs to qs to qs to qs to qs to 100 100 100 100 100 100 100 100 100

(5) (III) All Purpose Cleaner, Providing Bactericidal and Long Lasting Effects on Treated Surfaces

(6) TABLE-US-00009 INCI Name/ Chemical Trade name name Supplyer % w/w % w/w % w/w % w/w % w/w % w/w % w/w % w/w % w/w Part A Biocide 0.001 0.01 0.1 5 0.001 0.01 0.1 5 1 PEI (I), (II), 0.001 0.001 0.001 0.001 2 2 2 2 1 (III), (IV) Part B Plurafac Alkoxylated BASF 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 LF 400 Alcohol Sodium Sodium Hickson 5.00 5.00 5.00 5.00 5.00 5.00 5.00 5.00 5.00 Cumene Cumene Manro Sulfonate Sulfonate Marlon PS 65 Sodium Sasol 10.0 10.0 10.0 10.0 10.0 10.0 10.0 10.0 10.0 C13-17 Alkane Sulfonate Part C Perfume and q q q q q q q q q Preservative Water Aqua qs to qs to qs to qs to qs to qs to qs to qs to qs to 100 100 100 100 100 100 100 100 100 Trisodium Trisodium Fluka 5.00 5.00 5.00 5.00 5.00 5.00 5.00 5.00 5.00 Citrate Citrate Dihydrate Dihydrate
(IV) All Purpose Cleaner and Disinfectant, Provides Strong Immediate Bactericidal and Long Lasting Effects on Treated Surfaces Even at Low Concentrations

(7) TABLE-US-00010 INCI Name/ Trade name Chemical name Supplyer % w/w % w/w % w/w % w/w % w/w % w/w % w/w % w/w % w/w Part Biocide 0.001 0.01 0.1 5 0.001 0.01 0.1 5 1 A PEI (I), (II), (III), 0.001 0.001 0.001 0.001 2 2 2 2 1 (IV) Part Glucopon .sup.® 215 Alkyl BASF 3.00 3.00 3.00 3.00 3.00 3.00 3.00 3.00 3.00 B CS UP Polyglycoside Texapon .sup.® LS 35 Lauryl-myristyl BASF 10.00 10.00 10.00 10.00 10.00 10.00 10.00 10.00 10.00 sulfate Na-salt Sodium Cumene Sodium Hickson 5.00 5.00 5.00 5.00 5.00 5.00 5.00 5.00 5.00 Sulfonate Cumene Manro Sulfonate 2-Propanol Isopropyl Merck 10.00 10.00 10.00 10.00 10.00 10.00 10.00 10.00 10.00 Alcohol Part Perfume and qs 5.00 5.00 5.00 5.00 5.00 5.00 5.00 5.00 C Preservative Water Aqua qs to qs to qs to qs to qs to qs to qs to qs to qs to 100 100 100 100 100 100 100 100 100 Trisodium Citrate Trisodium Fluka 5.00 5.00 5.00 5.00 5.00 5.00 5.00 5.00 5.00 Dihydrate Citrate Dihydrate
(V) Bactericidal all Purpose Cleaner, Providing Strong Immediate Bactericidal and Long Lasting EfFects on Treated Surfaces

(8) TABLE-US-00011 INCI Name/ Chemical Trade name name Supplyer % w/w % w/w % w/w % w/w % w/w % w/w % w/w % w/w % w/w Part Biocide 0.001 0.01 0.1 5 0.001 0.01 0.1 5 1 A PEI (I), (II), 0.001 0.001 0.001 0.001 2 2 2 2 1 (III), (IV) Part Texapon .sup.® Lauryl-myristyl BASF 10.00 10.00 10.00 10.00 10.00 10.00 10.00 10.00 10.00 B LS 35 sulfate Na-salt Glucopon .sup.® Alkyl BASF 3.00 3.00 3.00 3.00 3.00 3.00 3.00 3.00 3.00 215 CS UP Polyglycoside Sodium Sodium Cumene Hickson 5.00 5.00 5.00 5.00 5.00 5.00 5.00 5.00 5.00 Cumene Sulfonate Manro Sulfonate Part Perfume and qs qs qs qs qs qs qs qs qs C Preservative Citric Acid Citric Acid Merck ad pH 3 ad pH 3 ad pH 3 ad pH 3 ad pH 3 ad pH 3 ad pH 3 ad pH 3 ad pH 3 Water Aqua qs to qs to qs to qs to qs to qs to qs to qs to qs to 100 100 100 100 100 100 100 100 100
(VI) Anti-Bacterial Fabric Softener Based on Conventional Quat

(9) TABLE-US-00012 INCI Name/ Chemical Trade name name Supplyer % w/w % w/w % w/w % w/w % w/w % w/w % w/w % w/w % w/w Part Biocide 0.001 0.01 0.1 5 0.001 0.01 0.1 5 1 A PEI (I), (II), (III), 0.001 0.001 0.001 0.001 2 2 2 2 1 (IV) Part Neodol 25-7 E C12-15 Pareth- Shell 0.50 0.50 0.50 0.50 0.50 0.50 0.50 0.50 0.50 B 7 Chemicals Arquad 2HT-75 Quaternium 18 Akzo 4.00 4.00 4.00 4.00 4.00 4.00 4.00 4.00 4.00 (and) Isopropyl Nobel Alcohol Part Water Aqua qs qs qs qs qs qs qs qs qs D Perfume and qs qs qs qs qs qs qs qs qs Preservative
(VII) Anti-Bacterial Fabric Softener Based on Esterquat

(10) TABLE-US-00013 INCI Name/ Chemical Trade name name Supplyer % w/w % w/w % w/w % w/w % w/w % w/w % w/w % w/w % w/w Part Biocide 0.001 0.01 0.1 5 0.001 0.01 0.1 5 1 A PEI (I), (II), (III), 0.001 0.001 0.001 0.001 2 2 2 2 1 (IV) Part Rewoquat WE 18 Dihydrogenated Evonik 4.00 0.50 0.50 0.50 0.50 0.50 0.50 0.50 0.50 B Tallowethyl Hydroxyethyla monium Neodol 25-7 E C12-15 Pareth- Shell 0.50 4.00 4.00 4.00 4.00 4.00 4.00 4.00 4.00 7 Chemicals Antifoam DB 31 Dow 0.10 qs qs qs qs qs qs qs qs Corning Part D Water Aqua qs to qs to qs to qs to qs to qs to qs to qs to qs to 100 100 100 100 100 100 100 100 100 Perfume and qs qs qs qs qs qs qs qs qs Preservative
(VIII) Antibacterial Heavy Duty Liquid Detergent

(11) TABLE-US-00014 INCI Name/ Chemical Trade name name Supplyer % w/w % w/w % w/w % w/w % w/w % w/w % w/w % w/w % w/w Part Biocide 0.001 0.01 0.1 5 0.001 0.01 0.1 5 1 A PEI (I), (II), (III), 0.001 0.001 0.001 0.001 2 2 2 2 1 (IV) Part Marlon A375 Sodium Sasol 15.00 15.00 15.00 15.00 15.00 15.00 15.00 15.00 15.00 B Dodecylbenzene- sulfonate Neodol 45-7 E C14-15 Pareth- Shell 14.00 14.00 14.00 14.00 14.00 14.00 14.00 14.00 14.00 7 Chemicals Alcohol SD Alcohol 39- Berkel 9.00 9.00 9.00 9.00 9.00 9.00 9.00 9.00 9.00 C Part Soap Base Sodium Tal- Mettler 10.00 10.00 10.00 10.00 10.00 10.00 10.00 10.00 10.00 C lowate (and) Sodium Co- coate Trisodium Citrate Trisodium Fluka 4.00 4.00 4.00 4.00 4.00 4.00 4.00 4.00 4.00 Dihydrate Citrate Dihydrate Triethanolamine Triethanolamine BASF 5.00 5.00 5.00 5.00 5.00 5.00 5.00 5.00 5.00 Part Tinopal .sup.® CBS-X Fluorescent BASF 0.10 0.10 0.10 0.10 0.10 0.10 0.10 0.10 0.10 D Whitening Agent (FWA) Part Water Aqua qs to qs to qs to qs to qs to qs to qs to qs to qs to E 100 100 100 100 100 100 100 100 100
(IX) Anti-Bacterial Heavy Duty Liquid Detergent

(12) TABLE-US-00015 INCI Name/ Chemical Trade name name Supplyer % w/w % w/w % w/w % w/w % w/w % w/w % w/w % w/w % w/w Part Biocide 0.001 0.01 0.1 5 0.001 0.01 0.1 5 1 A PEI (I), (II), (III), 0.001 0.001 0.001 0.001 2 2 2 2 1 (IV) Part Nansa SSA/A Dodecylbenzene Albright & 7.50 7.50 7.50 7.50 7.50 7.50 7.50 7.50 7.50 B Sulfonic Acid Wilson/ Rhodia Edenor .sup.® K12-18 Coconut Fatty BASF 12.50 12.50 12.50 12.50 12.50 12.50 12.50 12.50 12.50 Acid Neodol 23-6, 5 E C12-13 Pareth- Shell 10.00 10.00 10.00 10.00 10.00 10.00 10.00 10.00 10.00 7 Chemicals Elfan NS 243 S Sodium Akzo 17.00 17.00 17.00 17.00 17.00 17.00 17.00 17.00 17.00 Laureth Sulfate Nobel 3 EO Part 2-Propanol Isopropyl Merck 6.00 6.00 6.00 6.00 6.00 6.00 6.00 6.00 6.00 C Alcohol Alcohol SD Alcohol 39- Berkel 6.00 6.00 6.00 6.00 6.00 6.00 6.00 6.00 6.00 C Trisodium Citrate Trisodium Fluka 5.50 5.50 5.50 5.50 5.50 5.50 5.50 5.50 5.50 Dihydrate Citrate Dihydrate Sodium Water (and) Fluka 10.70 10.70 10.70 10.70 10.70 10.70 10.70 10.70 10.70 Hydroxide (30% Sodium solution) Hydroxide Part Water Aqua qs to qs to qs to qs to qs to qs to qs to qs to qs to D 100 100 100 100 100 100 100 100 100
(X) Antibacterial Liquid Laundry Detergent

(13) TABLE-US-00016 INCI Name/ Chemical Trade name name Supplyer % w/w % w/w % w/w % w/w % w/w % w/w % w/w % w/w % w/w Part Biocide 0.001 0.01 0.1 5 0.001 0.01 0.1 5 1 A PEI (I), (II), (III), 0.001 0.001 0.001 0.001 2 2 2 2 1 (IV) Part Marlon A 375 Sodium Sasol 8.7 8.7 8.7 8.7 8.7 8.7 8.7 8.7 8.7 B Dodecylbenzene- sulfonate Marlipal O 13/129 Trideceth-12 Sasol 3.50 3.50 3.50 3.50 3.50 3.50 3.50 3.50 3.50 Penta Sodium Penta Sodium Fluka 24.00 24.00 24.00 24.00 24.00 24.00 24.00 24.00 24.00 Triphosphate Triphosphate Sodium Sulfate Sodium Sulfate Merck 1.20 1.20 1.20 1.20 1.20 1.20 1.20 1.20 1.20 Sodium Water (and) Fluka 1.60 1.60 1.60 1.60 1.60 1.60 1.60 1.60 1.60 Hydroxide (10% Sodium solution) Hydroxide Glycerin Glycerin Merck 1.00 1.00 1.00 1.00 1.00 1.00 1.00 1.00 1.00 Oleic Acid Oleic Acid Fluka 0.90 0.90 0.90 0.90 0.90 0.90 0.90 0.90 0.90 Sodium Sodium Borate Fluka 1.80 1.80 1.80 1.80 1.80 1.80 1.80 1.80 1.80 Tetraborate Part Tylose C 10000 Cellulose Gum Clariant 0.40 0.40 0.40 0.40 0.40 0.40 0.40 0.40 0.40 C Part Water Aqua qs to qs to qs to qs to qs to qs to qs to qs to qs to D 100 100 100 100 100 100 100 100 100
(XI) Concentrated Anti-Bacterial Fabric Softener Based on Conventional Quat

(14) TABLE-US-00017 INCI Name/ Chemical % % % % % % % % % Trade name name Supplyer w/w w/w w/w w/w w/w w/w w/w w/w w/w Part A Biocide 0.001 0.01 0.1 5 0.001 0.01 0.1 5 1 PEI (I), (II), 0.001 0.001 0.001 0.001 2 2 2 2 1 (III), (IV) Part B Genapol O 100 Oleth-10 Clariant 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 Arquad 2HT-75 Quatemium Akzo 15.00 15.00 15.00 15.00 15.00 15.00 15.00 15.00 15.00 18 (and) Nobel Isopropyl Alcohol Part C 2-Propanol Isopropyl Merck 3.00 3.00 3.00 3.00 3.00 3.00 3.00 3.00 3.00 Alcohol Magnesium Magnesium Chloride Chloride 1.00 1.00 1.00 1.00 1.00 1.00 1.00 1.00 1.00 saturated solution Part D Water Aqua qs to qs to qs to qs to qs to qs to qs to qs to qs to 100 100 100 100 100 100 100 100 100 Perfume and qs qs qs qs qs qs qs qs qs Preservative
(XII) Concentrated Anti-Bacterial Fabric Softener Based on Esterquat

(15) TABLE-US-00018 INCI Name/ Chemical % % % % % % % % % Trade name name Supplyer w/w w/w w/w w/w w/w w/w w/w w/w w/w Part A Biocide 0.001 0.01 0.1 5 0.001 0.01 0.1 5 1 PEI (I), (II), 0.001 0.001 0.001 0.001 2 2 2 2 1 (III), (IV) Part B RewoquatWE 18 Dihydrogenated Evonik 15.00 15.00 15.00 15.00 15.00 15.00 15.00 15.00 15.00 Tallowethyl Hydroxyethyl amonium Genapol O 100 Oleth-10 Clariant 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 Part C Antifoam DB 31 Dow 0.50 0.50 0.50 0.50 0.50 0.50 0.50 0.50 0.50 Corning 2-Propanol Isopropyl Merck 3.00 3.00 3.00 3.00 3.00 3.00 3.00 3.00 3.00 Alcohol Magnesium Magnesium 1.00 1.00 1.00 1.00 1.00 1.00 1.00 1.00 1.00 Chloride Chloride saturated solution Part D Water Aqua qs to qs to qs to qs to qs to qs to qs to qs to qs to 100 100 100 100 100 100 100 100 100 Perfume and qs qs qs qs qs qs qs qs qs Preservative
(XIII) Antibacterial Surface Disinfectant Spray, Providing Strong Immediate BacteRicidal and Long Lasting Effects on Treated Surfaces

(16) TABLE-US-00019 INCI Name/ Chemical % % % % % % % % % Trade name name Supplyer w/w w/w w/w w/w w/w w/w w/w w/w w/w Part A Biocide 0.001 0.01 0.1 5 0.001 0.01 0.1 5 1 PEI (I), (II), 0.001 0.001 0.001 0.001 2 2 2 2 1 (III), (IV) Part B 2-Propanol Isopropyl Merck 10.00 10.00 10.00 10.00 10.00 10.00 10.00 10.00 10.00 Alcohol Texapon ® K12 Sodium Lauryl BASF 1.00 1.00 1.00 1.00 1.00 1.00 1.00 1.00 1.00 Sulfate Citric Acid Citric Acid Merck 3.00 3.00 3.00 3.00 3.00 3.00 3.00 3.00 3.00 Preservative qs qs qs qs qs qs qs qs qs Part C Sodium Water (and) Fluka ad pH ad pH ad pH ad pH ad pH ad pH ad pH ad pH ad pH Hydroxide Sodium 5.0 5.0 5.0 5.0 5.0 5.0 5.0 5.0 5.0 (10% solution) Hydroxide Part D Water Aqua qs to qs to qs to qs to qs to qs to qs to qs to qs to 100 100 100 100 100 100 100 100 100
(XIV) Surface Disinfectant Wet Wipes, Providing Strong Immediate Bactericidal and Long Lasting Effects on Treated Surfaces

(17) TABLE-US-00020 INCI Name/ Chemical % % % % % % % % % Trade name name Supplyer w/w w/w w/w w/w w/w w/w w/w w/w w/w Part A Biocide 0.001 0.01 0.1 5 0.001 0.01 0.1 5 1 PEI (I), (II), 0.001 0.001 0.001 0.001 2 2 2 2 1 (III), (IV) Part B Dowanol DPM PPG-2 Methyl Dow 1000 10.00 10.00 10.00 10.00 10.00 10.00 10.00 10.00 Ether Chemical Texapon ® K12 Sodium Lauryl BASF 1.00 1.00 1.00 1.00 1.00 1.00 1.00 1.00 1.00 Sulfate Citric Acid Citric Acid Merck 3.00 3.00 3.00 3.00 3.00 3.00 3.00 3.00 3.00 Preservative qs qs qs qs qs qs qs qs qs Part C Sodium Water (and) Fluka ad pH ad pH ad pH ad pH ad pH ad pH ad pH ad pH ad pH Hydroxide (10% 5.0 5.0 5.0 5.0 5.0 5.0 5.0 5.0 5.0 Sodium solution) Hydroxide Water Aqua qs qs qs qs qs qs qs qs qs
(XV) Surface Disinfectant Spray, Providing Strong Immediate Bactericidal and Long Lasting Effects on Treated Surfaces

(18) TABLE-US-00021 INCI Name/ Chemical % % % % % % % % % Trade name name Supplyer w/w w/w w/w w/w w/w w/w w/w w/w w/w Part A Biocide 0.001 0.01 0.1 5 0.001 0.01 0.1 5 1 PEI (I), (II), 0.001 0.001 0.001 0.001 2 2 2 2 1 (III), (IV) Part B Glucopon ® 215 Alkyl BASF 3.50 3.50 3.50 3.50 3.50 3.50 3.50 3.50 3.50 CS UP Polyglycoside Dehydol ® 04 Deo Capryleth-4 BASF 1.70 1.70 1.70 1.70 1.70 1.70 1.70 1.70 1.70 Part C Perfume and qs qs qs qs qs qs qs qs qs Preservative Acetic Acid conc. Acetic Acid Fluka 2.40 2.40 2.40 2.40 2.40 2.40 2.40 2.40 2.40 Water Aqua qs to qs to qs to qs to qs to qs to qs to qs to qs to 100 100 100 100 100 100 100 100 100 Citric Acid Citric Acid Merck 9.00 9.00 9.00 9.00 9.00 9.00 9.00 9.00 9.00
(XVI) Surface Disinfectant Spray, Providing Strong Immediate Bactericidal and Long Lasting Effects on Treated Surfaces

(19) TABLE-US-00022 INCI Name/ Chemical % % % % % % % % % Trade name name Supplyer w/w w/w w/w w/w w/w w/w w/w w/w w/w Part A Biocide 0.001 0.01 0.1 5 0.001 0.01 0.1 5 1 PEI (I), (II), (III), 0.001 0.001 0.001 0.001 2 2 2 2 1 (IV) Part B Glucopon ® 215 Alkyl BASF 3.50 3.50 3.50 3.50 3.50 3.50 3.50 3.50 3.50 CS UP Polyglycoside Dehydol ® 04 Deo Capryleth-4 BASF 1.70 1.70 1.70 1.70 1.70 1.70 1.70 1.70 1.70 Sodium Cumene Sodium Hickson 5.00 5.00 5.00 5.00 5.00 5.00 5.00 5.00 5.00 Sulfonate Cumene Manro Sulfonate Part C Perfume and qs qs qs qs qs qs qs qs qs Preservative Citric Acid Citric Acid Merck ad pH ad pH ad pH ad pH ad pH ad pH ad pH ad pH ad pH 3.0 3.0 3.0 3.0 3.0 3.0 3.0 3.0 3.0 Water Aqua qs to qs to qs to qs to qs to qs to qs to qs to qs to 100 100 100 100 100 100 100 100 100
(XVII) Surface Disinfectant Spray, Providing Strong Immediate Bactericidal and Long Lasting Effects on Treated Surfaces

(20) TABLE-US-00023 INCI Name/ Chemical % % % % % % % % % Trade name name Supplyer w/w w/w w/w w/w w/w w/w w/w w/w w/w Part A Biocide 0.001 0.01 0.1 5 0.001 0.01 0.1 5 1 PEI (I), (II), 0.001 0.001 0.001 0.001 2 2 2 2 1 (III), (IV) Part B Texapon ® K12 Sodium BASF 1.00 1.00 1.00 1.00 1.00 1.00 1.00 1.00 1.00 Lauryl Sulfate Citric Acid Citric Acid Merck 3.00 3.00 3.00 3.00 3.00 3.00 3.00 3.00 3.00 Preservative qs qs qs qs qs qs qs qs qs Dowanol DPM PPG-2 Dow 1000 10.00 10.00 10.00 10.00 10.00 10.00 10.00 10.00 Methyl Ether Chemical Part C Sodium Water (and) Fluka ad pH ad pH ad pH ad pH ad pH ad pH ad pH ad pH ad pH Hydroxide Sodium 5.0 5.0 5.0 5.0 5.0 5.0 5.0 5.0 5.0 (10% solution) Hydroxide
(XVIII) Surface Disinfectant Spray, Providing Strong Immediate Bactericidal and Long Lasting Effects on Treated Surfaces.

(21) Formulation can be diluted: 1 part to 9 parts of water

(22) TABLE-US-00024 INCI Name/ Chemical % % % % % % % % % Trade name name Supplyer w/w w/w w/w w/w w/w w/w w/w w/w w/w Part A Biocide 0.001 0.01 0.1 5 0.001 0.01 0.1 5 1 PEI (I), (II), 0.001 0.001 0.001 0.001 2 2 2 2 1 (III), (IV) Part B Texapon ® K12 Sodium BASF 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 Lauryl Sulfate 1-Propanol Propyl Merck 10.00 10.00 10.00 10.00 10.00 10.00 10.00 10.00 10.00 Alcohol Part C Hydrogen Hydrogen Merck 10.00 10.00 10.00 10.00 10.00 10.00 10.00 10.00 10.00 Peroxide 30% Peroxide Part D Citric Acid Citric Merck ad pH ad pH ad pH ad pH ad pH ad pH ad pH ad pH ad pH sol. 20% Acid 3.0 3.0 3.0 3.0 3.0 3.0 3.0 3.0 3.0 Preservative qs qs qs qs qs qs qs qs qs Part E Water Aqua qs to qs to qs to qs to qs to qs to qs to qs to qs to 100 100 100 100 100 100 100 100 100
(XIX) Surface Disinfectant Spray, Providing Immediate Bactericidal and Long LastIng Effects on Treated Surfaces

(23) TABLE-US-00025 INCI Name/ Chemical % % % % % % % % % Trade name name Supplyer w/w w/w w/w w/w w/w w/w w/w w/w w/w Part A Biocide 0.001 0.01 0.1 5 0.001 0.01 0.1 5 1 PEI (I), (II), 0.001 0.001 0.001 0.001 2 2 2 2 1 (III), (IV) Part B 2-Propanol Isopropyl Merck 10.00 10.00 10.00 10.00 10.00 10.00 10.00 10.00 10.00 Alcohol Glucopon ® Alkyl BASF 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 215 CS UP Polyglycoside Part C Perfume and qs qs qs qs qs qs qs qs qs Preservative Water Aqua qs to qs to qs to qs to qs to qs to qs to qs to qs to 100 100 100 100 100 100 100 100 100 Citric Acid Citric Acid Merck 1.00 1.00 1.00 1.00 1.00 1.00 1.00 1.00 1.00 Sodium Water (and) Fluka ad pH ad pH ad pH ad pH ad pH ad pH ad pH ad pH ad pH Hydroxide Sodium 5.0 5.0 5.0 5.0 5.0 5.0 5.0 5.0 5.0 (10% solution) Hydroxide
(XX) Surface Disinfectant Wet Wipes, Providing Immediate Bactericidal and Long Lasting Effects on Treated Surfaces

(24) TABLE-US-00026 INCI Name/ Chemical % % % % % % % % % Trade name name Supplyer w/w w/w w/w w/w w/w w/w w/w w/w w/w Part A Biocide 0.001 0.01 0.1 5 0.001 0.01 0.1 5 1 PEI (I), (II), 0.001 0.001 0.001 0.001 2 2 2 2 1 (Ill), (IV) Part B 2-Propanol Isopropyl Merck 10.00 10.00 10.00 10.00 10.00 10.00 10.00 10.00 10.00 Alcohol Glucopon ® Alkyl BASF 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 215 CS UP Polyglycoside Part C Perfume and qs qs qs qs qs qs qs qs qs Preservative Water Aqua qs to qs to qs to qs to qs to qs to qs to qs to qs to 100 100 100 100 100 100 100 100 100 Citric Acid Citric Acid Merck 1.00 1.00 1.00 1.00 1.00 1.00 1.00 1.00 1.00 Sodium Water (and) Fluka ad pH ad pH ad pH ad pH ad pH ad pH ad pH ad pH ad pH Hydroxide Sodium 5.0 5.0 5.0 5.0 5.0 5.0 5.0 5.0 5.0 (10% solution) Hydroxide
(XXI) Antibacterial Window Cleaner, Providing Immediate Bactericidal and Long Lasting Effects on Treated Surfaces

(25) TABLE-US-00027 INCI Name/ Chemical % % % % % % % % % Trade name name Supplyer w/w w/w w/w w/w w/w w/w w/w w/w w/w Part A Biocide 0.001 0.01 0.1 5 0.001 0.01 0.1 5 1 PEI (I), (II), 0.001 0.001 0.001 0.001 2 2 2 2 1 (III), (IV) Part B 2-Propanol Isopropyl Merck 10.00 10.00 10.00 10.00 10.00 10.00 10.00 10.00 10.00 Alcohol Glucopon ® Alkyl BASF 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 215 CS UP Polyglycoside Part C Perfume and qs qs qs qs qs qs qs qs qs Preservative Water Aqua qs to qs to qs to qs to qs to qs to qs to qs to qs to 100 100 100 100 100 100 100 100 100 Citric Acid Citric Acid Merck 1.00 1.00 1.00 1.00 1.00 1.00 1.00 1.00 1.00 Sodium Water (and) Fluka ad pH ad pH ad pH ad pH ad pH ad pH ad pH ad pH ad pH Hydroxide Sodium 5.0 5.0 5.0 5.0 5.0 5.0 5.0 5.0 5.0 (10% solution) Hydroxide
(XXII) Bactericidal Dishwashing Liquid, Providing Strong Bactericidal and Long Lasting Antibacterial Protection of Dishwashing Utensils

(26) TABLE-US-00028 INCI Name/ Chemical % % % % % % % % % Trade name name Supplyer w/w w/w w/w w/w w/w w/w w/w w/w w/w Part A Biocide 0.001 0.01 0.1 5 0.001 0.01 0.1 5 1 PEI (I), (II), 0.001 0.001 0.001 0.001 2 2 2 2 1 (III), (IV) Part B Marlon A 375 Sodium Sasol 7.00 7.00 7.00 7.00 7.00 7.00 7.00 7.00 7.00 Dodecyl benzenesulfonate Sodium Sodium Hickson 5.00 5.00 5.00 5.00 5.00 5.00 5.00 5.00 5.00 Cumene Cumene Manro Sulfonate Sulfonate Part C Citric Acid Citric Acid Merck 3.00 3.00 3.00 3.00 3.00 3.00 3.00 3.00 3.00 Benzoic Acid Benzoic Acid Merck 0.50 0.50 0.50 0.50 0.50 0.50 0.50 0.50 0.50 Sodium Sodium Fluka 1.00 1.00 1.00 1.00 1.00 1.00 1.00 1.00 1.00 Chloride Chloride Sodium Sodium Merck 3.50 3.50 3.50 3.50 3.50 3.50 3.50 3.50 3.50 Sulfate Sulfate Sodium Water (and) Fluka ad pH ad pH ad pH ad pH ad pH ad pH ad pH ad pH ad pH Hydroxide Sodium 5.0 5.0 5.0 5.0 5.0 5.0 5.0 5.0 5.0 (30% solution) Hydroxide Part D Water Aqua qs qs qs qs qs qs qs qs qs
(XVII) High Foaming and Mild Dishwashing Liquid, Providing Strong Bactericidal and Long Lasting Antibacterial Protection of Dishwashing Utensils and Sponges

(27) TABLE-US-00029 INCI Name/ Chemical % % % % % % % % % Trade name name Supplyer w/w w/w w/w w/w w/w w/w w/w w/w w/w Part A Biocide 0.001 0.01 0.1 5 0.001 0.01 0.1 5 1 PEI (I), (II), 0.001 0.001 0.001 0.001 2 2 2 2 1 (III), (IV) Part B Texapon ® LS 35 Lauryl-myristyl BASF 7.00 7.00 7.00 7.00 7.00 7.00 7.00 7.00 7.00 sulfate Na-salt Texapon ® K14 Sodium Myreth BASF 7.00 7.00 7.00 7.00 7.00 7.00 7.00 7.00 7.00 Special Sulfate Plantacare ® 1200 Lauryl BASF 4.00 4.00 4.00 4.00 4.00 4.00 4.00 4.00 4.00 UP Glucoside Dehyton ® AB 30 Coco Betaine BASF 1.1 1.1 1.1 1.1 1.1 1.1 1.1 1.1 1.1 Alcohol SD Alcohol 39-C Berkel 5.00 5.00 5.00 5.00 5.00 5.00 5.00 5.00 5.00 Part C Sodium Chloride Sodium Fluka 1.00 1.00 1.00 1.00 1.00 1.00 1.00 1.00 1.00 Chloride Part D Citric Acid Citric Acid Merck ad pH ad pH ad pH ad pH ad pH ad pH ad pH ad pH ad pH 6.5 6.5 6.5 6.5 6.5 6.5 6.5 6.5 6.5 Preservative qs qs qs qs qs qs qs qs qs Water Aqua qs qs qs qs qs qs qs qs qs
(XXIII) Disinfectant Toilet Cleaner, Providing Strong Immediate Bactericidal and Long Lasting Effects on Treated Surfaces

(28) TABLE-US-00030 INCI Name/ Chemical % % % % % % % % % Trade name name Supplyer w/w w/w w/w w/w w/w w/w w/w w/w w/w Part A Biocide 0.001 0.01 0.1 5 0.001 0.01 0.1 5 1 PEI (I), (II), 0.001 0.001 0.001 0.001 2 2 2 2 1 (III), (IV) Glucopon ® 215 Alkyl BASF 3.50 3.50 3.50 3.50 3.50 3.50 3.50 3.50 3.50 CS UP Poly glycoside Neodol 91-8E 9-11 pareth-8 Caldic 1.70 1.70 1.70 1.70 1.70 1.70 1.70 1.70 1.70 Part B RHEOVIS ® Cationic BASF 1.30 1.30 1.30 1.30 1.30 1.30 1.30 1.30 1.30 CSP Polymer Part C Acetic Acid conc. Acetic Acid Fluka 2.40 2.40 2.40 2.40 2.40 2.40 2.40 2.40 2.40 Citric Acid Citric Acid Merck 9.00 9.00 9.00 9.00 9.00 9.00 9.00 9.00 9.00 PartD IRAGON ® Blue Acid blue 80 BASF 0.50 0.50 0.50 0.50 0.50 0.50 0.50 0.50 0.50 ABL80 sol. 1% Water Aqua qs to qs to qs to qs to qs to qs to qs to qs to qs to 100 100 100 100 100 100 100 100 100
(XXIV) Shoe Deodorant (Alcohol Based Spray Formulation)

(29) TABLE-US-00031 INCI Name/ Chemical % % % % % % % % % Trade name name Supplyer w/w w/w w/w w/w w/w w/w w/w w/w w/w Part A Biocide 0.001 0.01 0.1 5 0.001 0.01 0.1 5 1 PEI (I), (II), 0.001 0.001 0.001 0.001 2 2 2 2 1 (III), (IV) Part B Ethanol absolut. Alcohol Riedel 20.00 20.00 20.00 20.00 20.00 20.00 20.00 20.00 20.00 Propylene Propylene BASF 20.00 20.00 20.00 20.00 20.00 20.00 20.00 20.00 20.00 Glycol Glycol Marlipal 24/99 Laureth-9 Sasol 1.00 1.00 1.00 1.00 1.00 1.00 1.00 1.00 1.00 Glycerin Glycerin Merck 1.00 1.00 1.00 1.00 1.00 1.00 1.00 1.00 1.00 Perfume qs qs qs qs qs qs qs qs qs Part C Water Aqua qs to qs to qs to qs to qs to qs to qs to qs to qs to 100 100 100 100 100 100 100 100 100 Biocides used in each of the above formulations (I) to (XXIV) are: Bronopol, Phenoxyethanol, Glutaraldehyde, Formic Acid, Glyoxal, 2,4-Dichlorobenzylalcohol, Dazomet (3,5-Dimetyl-1,3-5-thiadiazinane-2-thione), 1,3,5-Tris-(2-hydroxyethyl)-1,3,5-hexahydrotriaxine, Cybutryne (2-Methylthio-4-tert-butylamino cyclopropylamino-6-(1,3,5-triazine)), Irgaguard ® B5000, Irgaguard ® B6000, Irgaguard ® B7000, Triclosan, 4,4′-Dichloro 2′-hydroxydiphenylether (Diclosan, a.i. of Tinosan ®HP 100).

(30) The following examples illustrate the invention. Wherever noted, room temperature (r.t.) depicts a temperature from the range 22-25° C.; over night means a period of 12 to 15 hours; percentages are given by weight, if not indicated otherwise.

Abbreviations

(31) HPLC high pressure liquid chromatography

(32) Mw molecular weight (usually as detected by GPC)

(33) GPC gel permeation chromatography

(34) DSC differential scanning calorimetry

(35) NMR nuclear magnetic resonance

(36) a.i. active ingredient

Example 1

(37) Microbiological data demonstrating synergistic efficacy of biocides (reduction of the number of viable cells expressed as common logarythm (log redn.)) in combinations with Polyethylenimines are summarized in the following tables. Lines in grey shading indicate control sample (no PEI or no biocide added):

(38) TABLE-US-00032 TABLE 1 Examples for synergistic bactericidal effects of biocides in combination with Polyethylenimines. The bactericidal activities are determined according to to method EN1040 against Pseudomonas aeruginosa ATCC 15442. log redn. after Conc. of PEI Conc. of Biocide Contact time [ppm [% 1 3 6 24 PEI a.i.] Biocide a.i.] hr hrs hrs hrs Lupasol .sup.® 100 2.3 3.2 4.1 >6 FG Phenoxyethanol 1 <1 1.3 2.8 >6 Lupasol .sup.® 100 Phenoxyethanol 1 >5 >5 >5 >5 FG PEI (II) 50 3.5 4.4 >5 >6 Phenoxyethanol 1 <1 1.3 2.8 >6 PEI (II) 50 Phenoxyethanol 1 >5 >5 >5 >5 Lupasol .sup.® 50 >5 5.0 5 >5 WF Phenoxyethanol 1 <1 1.3 2.8 >6 Lupasol .sup.® 50 Phenoxyethanol 1 >5 5.0 5.0 >5 WF PEI (IV) 80 <1 <1 <1 >1 Phenoxyethanol 1 <1 1.3 2.8 >6 PEI (IV) 80 Phenoxyethanol 1 >5 >5 >5 >5 PEI (IV) 80 <1 <1 <1 >6 Bronopol 0.1 <1 <1 4.2 2.4 PEI (IV) 80 Bronopol 0.1 <1 1.7 >5 >5

(39) TABLE-US-00033 TABLE 2 Examples for synergistic bactericidal effects of biocides in combination with Polyethylenimines. The bactericidal activities are determined according to to method EN1040 against Staphylococcus aureus ATCC 6538. log redn. after Conc. of PEI Conc. of Biocide Contact time [ppm [% 1 3 6 24 PEI a.i.] Biocide a.i.] hr hrs hrs hrs Lupasol .sup.® 100 <1 1.2 1.2 2.3 FG Phenoxyethanol 1 <1 <1 <1 1.1 Lupasol .sup.® 100 Phenoxyethanol 1 1.0 1.7 4.5 >5 FG PEI (II) 50 1.3 2.1 1.9 4.4 Phenoxyethanol 1 <1 <1 <1 1.1 PEI (II) 50 Phenoxyethanol 1 1.3 4.4 >5 >5 Lupasol .sup.® 50 <1 2.1 3.1 >5 WF Phenoxyethanol 1 <1 <1 <1 1.1 Lupasol .sup.® 50 Phenoxyethanol 1 >5 >5 >5 >5 WF PEI (IV) 50 <1 <1 <1 <1 Phenoxyethanol 1 <1 <1 <1 1.1 PEI (IV) 50 Phenoxyethanol 1 <1 <1 <1 4.2 PEI (IV) 80 <1 <1 <1 >1 Bronopol 0.1 <1 <1 <1 2.4 PEI (IV) 80 Bronopol 0.1 <1 <1 <1 >5

(40) TABLE-US-00034 TABLE 3 Boosting efficacy of Polyethylenimines and Tinosan .sup.® HP 100. The bactericidal activities are determined according to to method EN1276 against Staphylococcus aureus ATCC 6538. Conc. of PEI log redn. after [ppm Conc. of Biocide Contact time PEI a.i.] Biocide [ppm a.i.] 5 min Tinosan .sup.® HP 100 250 4.9 Lupasol .sup.® FG 100 Tinosan .sup.® HP 100 250 5.1 Lupasol .sup.®FG 1000 Tinosan .sup.® HP 100 250 5.4

(41) An independent study (control) shows that 1000 ppm (a.i.) Lupasol® FG alone has no bactericidal activity at a contact time of 5 minutes (<1 log reduction) against S. aureus ATCC 6538.

Example 2

(42) Preservation Challenge Test according to the test method for topical products described in Eur. Pharm. edition 7.1 against several typical bacterial or fungal spoilage microorganisms. A surfactant formulation (45% Cocoamidopropyl betaine in water) is preserved with 0.5% phenoxyethanol alone or in combination with 0.1% polyethyleneimine (referring to active ingredient).

(43) Samples are inoculated with 1.5 to 3.5×105 cfu/ml as indicated in Table 5 below. After 7 days of incubation at 20° C., samples are taken and the total viable count is determined. Log reductions are calculated with reference to the initial germ load. The results show that the combination of phenoxyethanol with polyethylenimines perform better with regard to antimicrobial activity than phenoxyethanol alone. Table 4 shows the composition of formulations, Table 5 shows antimicrobial efficacies (log red.) after 7 d of contact time.

(44) TABLE-US-00035 TABLE 4 Formulations Sample 25 Sample 26 Sample 27 Sample 28 Ingredient (comparison) (invention) (invention) (invention) surfactant formulation 11.11% 11.11% 11.11% 11.11% phenoxyethanol  0.5%  0.5%  0.5%  0.5% Lupasol .sup.® WF —  0.1% — — Lupasol .sup.® FG — —  0.1% — PEI (IV) — — —  0.1% Water to 100 % to 100% to 100% to 100% pH is adjusted to 7 by addition of citric acid and/or NaOH.

(45) TABLE-US-00036 TABLE 5 Antimicrobial effect (log red.) after 7 days of contact time Sample Sample 28 Initial germ 25 Sample 26 Sample 27 (inven Test organism load [cfu/ml] (comp.) (invention) (invention) -tion) P. aeruginosa 2.5 × 10.sup.5 ≤1 ≥3 ≥3 ≤3 ATCC 9027 S. aureus 1.9 × 10.sup.5 ≥3 5 5 3 ATCC 6538 C. albicans 3.4 × 10.sup.5 ≥4 6 6 6 ATCC 10231

Example 3: Bactericidal Effect

(46) Bactericidal activities are determined according to method EN1040 against Staphylococcus aureus ATCC 6538, Pseudomonas aeruginosa ATCC 15442 and Escherichia coli ATCC 10536. Suspensions adjusted to pH 4, pH 7 and pH 9 by addition of citric acid and/or NaOH are used. Bactericidal efficacies are given in the following tables as log reduction calculated from total viable counts compared to the water control. The microbicidal activities are determined after the following contact times: 5 minutes, 15 minutes, 30 minutes, 60 minutes, 3 hours, 6 hours and 24 hours. Effects (log red.) by bactericide alone, polyethylenimine alone, and by the combination of bactericide and polyethylenimine are compiled in Tables 6 (Lupasol® FG), Table 7 (Lupasol® WF) and Table 8 (PEI (IV)).

(47) TABLE-US-00037 TABLE 6 Bactericidal effect on Staphylococcus aureus (S), Pseudomonas aeruginosa (P) and Escherichia coli (E) by combination of biocide with Lupasol .sup.® FG log redn. Biocide log redn. at Polymer Active alone Active Concentration Conc. (compar- Lupasol .sup.® FG Biocide [ppm] ison) [1000 ppm] [100 ppm] [10 ppm] 5-chloro-2-(4- 10000 chlorophenoxy) 1000 phenol 100 10 1 0.1 2, 4, 4’- 10000 trichloro-2’ 1000 hydroxyl- 100 diphenylether 10 1 0.1 1,5- 10000 pentanedial 5000 2500 500 50 5 1,2-ethanedial 200000 100000 20000 4000 2000 400 formic acid 20000 16000 8000 4000 1000 500 2-bromo-2- 10000 nitropropane- 5000 1,3-diol 1000 500 100 50 2-phenoxy-1- 10000 ethanol 5000 1000 500 100 50 Polymer (comp.)

(48) TABLE-US-00038 TABLE 7 Bactericidal effect (log redn.) on Staphylococcus aureus (S), Pseudomonas aeruginosa (P) and Escherichia coli (E) by combination of biocide with Lupasol .sup.® WF Biocide Polymer Active Active alone Concentration Conc. (compar- Lupasol .sup.® FG Biocide [ppm] ison) [1000 ppm] [100 ppm] [10 ppm] 5-chloro-2-(4- 10000 chlorophenoxy) 1000 phenol 100 10 1 0.1 2, 4, 4’- 10000 trichloro-2’ 1000 hydroxyl- 100 diphenylether 10 1 0.1 1,5- 10000 pentanedial 5000 2500 500 50 5 1,2-ethanedial 200000 100000 20000 4000 2000 400 formic acid 20000 16000 8000 4000 1000 500 2-bromo-2- 10000 nitropropane- 5000 1,3-diol 1000 500 100 50 2-phenoxy-1- 10000 ethanol 5000 1000 500 100 50 Polymer alone (compar- ison)

(49) TABLE-US-00039 TABLE 8 Bactericidal effect (log redn.) on Staphylococcus aureus (S), Pseudomonas aeruginosa (P) and Escherichia coli (E) by combination of biocide with PEI (IV) Biocide Polymer Active Active alone Concentration Conc. (compar- PEI (IV) Biocide [ppm] ison) [1000 ppm] [100 ppm] [10 ppm] 5-chloro-2-(4- 10000 chlorophenoxy) 1000 phenol 100 10 1 0.1 2, 4, 4’- 10000 trichloro-2’ 1000 hydroxyl- 100 diphenylether 10 1 0.1 1,5- 10000 pentanedial 5000 2500 500 50 5 1,2-ethanedial 200000 100000 20000 4000 2000 400 formic acid 20000 16000 8000 4000 1000 500 2-bromo-2- 10000 nitropropane- 5000 1,3-diol 1000 500 100 50 2-phenoxy-1- 10000 ethanol 5000 1000 500 100 50 Polymer alone (compar- ison)

Example 4: Fungicidal Effect

(50) Fungicidal activities are determined according to method EN1275 against Candida albicans ATCC 10231 and Aspergillus brasiliensis ATCC 16404. Suspensions adjusted to pH 4, pH 7 and pH 9 by addition of citric acid and/or NaOH are used. Efficacies are given as log reduction calculated from total viable counts compared to the water control. The microbicidal activities are determined after the following contact times: 5 minutes, 15 minutes, 30 minutes, 60 minutes, 3 hours, 6 hours and 24 hours. Fungicidal effect (log red.) by the biocide alone, polyethylenimine alone, and by the combination of biocide and polyethylenimine are compiled in Tables 9 (Lupasol® FG), Table 10 (Lupasol® WF) and Table 11 (PEI (IV)).

(51) TABLE-US-00040 TABLE 9 Fungicidal effect (log redn.) on Candida albicans (C) and Aspergillus brasili- ensis (A) by combination of biocide with Lupasol .sup.® FG Biocide Polymer Active Active alone Concentration Conc. (compar- Lupasol .sup.® FG Biocide [ppm] ison) [1000 ppm] [100 ppm] [10 ppm] 5-chloro-2-(4- 10000 chlorophenoxy) 1000 phenol 100 10 1 0.1 2, 4, 4’- 10000 trichloro-2’ 1000 hydroxyl- 100 diphenylether 10 1 0.1 1,5- 10000 pentanedial 5000 2500 500 50 5 1,2-ethanedial 200000 100000 20000 4000 2000 400 formic acid 20000 16000 8000 4000 1000 500 10000 2-bromo-2- 5000 nitropropane- 1000 1,3-diol 500 100 50 2-phenoxy-1- 10000 ethanol 5000 1000 500 100 50 Polymer alone (compar- ison)

(52) TABLE-US-00041 TABLE 10 Fungicidal effect (log redn.) on Candida albicans (C) and Aspergillus brasili- ensis (A) by combination of biocide with Lupasol .sup.® WF Biocide Polymer Active Active alone Concentration Conc. (compar- Lupasol .sup.® WF Biocide [ppm] ison) [1000 ppm] [100 ppm] [10 ppm] 5-chloro-2-(4- 10000 chlorophenoxy) 1000 phenol 100 10 1 0.1 2, 4, 4’- 10000 trichloro-2’ 1000 hydroxyl- 100 diphenylether 10 1 0.1 1,5- 10000 pentanedial 5000 2500 500 50 5 1,2-ethanedial 200000 100000 20000 4000 2000 400 formic acid 20000 16000 8000 4000 1000 500 2-bromo-2- 10000 nitropropane- 5000 1,3-diol 1000 500 100 50 2-phenoxy-1- 10000 ethanol 5000 1000 500 100 50 Polymer alone (compar- ison)

(53) TABLE-US-00042 TABLE 11 Fungicidal effect (log redn.) on Candida albicans (C) and Aspergillus brasili- ensis (A) by combination of biocide with PEI (IV) Biocide Polymer Active Active alone Concentration Conc. (compar- PEI (IV) Biocide [ppm] ison) [1000 ppm] [100 ppm] [10 ppm] 5-chloro-2-(4- 10000 chlorophenoxy) 1000 phenol 100 10 1 0.1 2, 4, 4’- 10000 trichloro-2’ 1000 hydroxyl- 100 diphenylether 10 1 0.1 1,5- 10000 pentanedial 5000 2500 500 50 5 1,2-ethanedial 200000 100000 20000 4000 2000 400 formic acid 20000 16000 8000 4000 1000 500 2-bromo-2- 10000 nitropropane- 5000 1,3-diol 1000 500 100 50 2-phenoxy-1- 10000 ethanol 5000 1000 500 100 50 Polymer alone (compar- ison)