FULL-AUTOMATIC BIOCHEMICAL ANALYZER, AND SAMPLING DEVICE AND SAMPLING METHOD THEREOF

Abstract

The present invention provides an auto biochemical analyzer and a sampling device and a sampling method thereof. The auto biochemical analyzer comprises a frame (100), a horizontal motion system, a vertical motion system, a sampling component and a transfer guide track (101), wherein the horizontal motion system comprises a first stepping motor (116); the vertical motion system comprises a second stepping motor (103); the first stepping motor (116) and the second stepping motor (103) are fixedly installed on the frame (100) respectively; the sampling component comprises a sampling needle holder block (114) and a sampling needle (111); and the stepping motors (116, 103) are both fixed to the frame. The analyzer is smart in motion, low in cost and compact in structure, and has the functions of open sampling and closed puncturing as well as conveying a sample to a specific position.

Claims

1. An auto biochemical analyzer, comprising a frame, a horizontal motion system, a vertical motion system, a sampling component and a transfer guide track, wherein the horizontal motion system comprises a first stepping motor; the vertical motion system comprises a second stepping motor; the first stepping motor and the second stepping motor are fixedly installed on the frame respectively; the said sampling component comprises a sampling needle holder block and a sampling needle; the second stepping motor and the sampling component are separately arranged; the sampling needle holder block comprises a guiding column; a guide track holder block is provided with a hole matched with the guiding column at a corresponding position; and when the sampling component is in contact with the guide track holder block, the guiding column is received in the hole to keep the close contact between the sampling component and the holder block.

2. The auto biochemical analyzer of claim 1, wherein the vertical motion system further comprises a second synchronous belt, a first guiding shaft parallel to the second synchronous belt and the guide track holder block capable of sliding on the first guiding shaft, the guide track holder block being fixedly connected with the second synchronous belt.

3. The auto biochemical analyzer of claim 1, wherein when the sampling component comes into contact with the guide track holder block, the sampling needle holder block combines with the guide track holder block through the guiding column and the conical hole, and then the sampling needle holder block, together with the guide track holder block, drives the sampling needle to execute tube puncturing and sample aspirating actions under the traction of the second synchronous belt, and after the sample aspirating action is completed, the sampling needle holder block breaks away from the guide track holder block, and then drives the sampling needle to convey the sample in the horizontal direction.

4. The auto biochemical analyzer of 1, wherein further comprising a transfer guide track, one end of said transfer guide track is in rigid connection with the guide track holder block, and the other end of the transfer guide track is fixedly connected with the sampling needle holder block.

5. The auto biochemical analyzer of claim 1, wherein the sampling needle respectively stays at a sampling position, a reaction cell position and a counting cell position, and the sampling position is closer to the second stepping motor than the reaction cell position and the counting cell position.

6. A sampling device of an auto biochemical analyzer, comprising a frame, a horizontal motion system, a vertical motion system, a sampling component and a transfer guide track, the horizontal motion system comprises a first stepping motor; the vertical motion system comprises a second stepping motor; the first stepping motor and the second stepping motor are fixedly installed on the frame respectively; the sampling component comprises a sampling needle holder block and a sampling needle; the transfer guide track is horizontally placed and is in rigid connection with a specified component of the vertical motion system; the sampling component is in sliding connection with the transfer guide track; the horizontal motion system drives the sampling needle to move in the horizontal direction; and the vertical motion system drives the sampling needle to move in the vertical direction.

7. The sampling device of claim 6, wherein the horizontal motion system further comprises a first synchronous belt and a horizontal guide track; the vertical motion system further comprises a second synchronous belt, a first guiding shaft and a guide track holder block, the guide track holder block being fixedly connected with the second synchronous belt; and the sampling component further comprises a sampling component bracket and a second guiding shaft.

8. The sampling device of claim 7, wherein the sampling component bracket is fixedly connected with the first synchronous belt, and the sampling component bracket is fixed to a sliding block of the horizontal guide track; under the drive of the first stepping motor, the sampling component moves in the horizontal direction under the guide of the horizontal guide track; and the sliding block of the transfer guide track is fixedly connected with the sampling needle holder block, and the sampling needle holder block moves in the vertical direction with the transfer guide track under the drive of the second stepping motor.

9. The sampling device of claim 7, wherein the guide track holder block is fixedly connected with the transfer guide track.

10. The sampling device of claim 7, wherein the sampling needle holder block comprises a partial cone-shaped guiding column, and the guide track holder block is provided with a hole matched with the guiding column.

11. (canceled)

12. The sampling device of claim 6, further comprising an optocoupler switch which that is installed on the frame, and wherein optocoupler switch sensor chips are respectively installed on the sampling component bracket and the guide track holder block.

13. (canceled)

14. (canceled)

15. (canceled)

16. A sampling method using an auto biochemical analyzer, said auto biochemical analyzer comprising a sampling device, wherein the sampling device comprises a frame, a horizontal motion system, a vertical motion system, a sampling component and a transfer guide track, wherein the horizontal motion system comprises a first stepping motor; the vertical motion system comprises a second stepping motor; the first stepping motor and the second stepping motor are installed on the frame respectively; the sampling component comprises a sampling needle holder block and a sampling needle; and the transfer guide track is horizontally placed and is fixedly connected with the vertical motion system and the sampling needle holder block respectively, the method comprising the following steps: a) starting the first stepping motor to drive the sampling component to move to a sampling position in the horizontal direction, and turning off the first stepping motor; b) starting the second stepping motor to drive the sampling needle to move in the vertical direction and penetrate through a container containing a sample, and return to the sampling position after aspirating the sample, and turning off the second stepping motor; c) starting the first stepping motor to drive the sampling component to move to a reaction cell position in the horizontal direction, and turning off the first stepping motor; d) starting the second motor to drive the sampling needle to move in the vertical direction and inject the sample into a reaction cell, and after a preset reaction time, to drive the sampling needle to aspirate part of mixed liquid after reaction and move to the reaction cell position, and turning off the second motor; e) starting the first motor to drive the sampling needle to move to a counting cell position in the horizontal direction, and turning off the first motor; f) starting the second motor to drive the sampling needle to move in the vertical direction and inject the part of the mixed liquid after reaction aspirated by the sampling needle into a counting cell and to drive the sampling needle to return to the counting cell position, and turning off the second motor; and g) starting the first motor to drive the sampling needle to move to an initial position in the horizontal direction, and turning off the first motor.

17. The method of claim 16, wherein the horizontal motion system further comprises a first synchronous belt and a horizontal guide track; the vertical motion system further comprises a second synchronous belt, a first guiding shaft and a guide track holder block, the guide track holder block being fixedly connected with the second synchronous belt; and the sampling component further comprises a sampling component bracket and a second guiding shaft.

18. The method of claim 17, wherein a first synchronous belt wheel is installed on a rotating shaft of the first stepping motor; the first synchronous belt moves in the horizontal direction under the drive of the first synchronous belt wheel; the sampling component bracket is fixedly connected with the first synchronous belt; the sampling component bracket is fixedly connected with a sliding block of the transfer guide track; the sliding block of the transfer guide track is fixedly connected with the sampling needle holder block; the transfer guide track and the horizontal guide track are placed in parallel; and when the first synchronous belt moves in the horizontal direction, the sampling component performs horizontal reciprocating motion under the guide of both the horizontal guide track and the transfer guide track.

19. The method of claim 16, wherein the guide track holder block and the sampling needle holder block vertically slide on the first guiding shaft and the second guiding shaft respectively; the guide track holder block is fixedly connected with the synchronous belt; the sliding block of the transfer guide track is fixedly connected with the sampling needle holder block; when the second synchronous belt moves in the vertical direction under the drive of the second stepping motor, the guide track holder block indirectly drives, through the transfer guide track, the sampling needle holder block to perform vertical reciprocating motion under the guide of the first guiding shaft and the second guiding shaft; the sampling needle is fixedly connected with the sampling needle holder block; and the sampling needle performs vertical lifting motion with the transfer guide track under the drive of the second synchronous belt so as to accomplish a sample aspirating action and a sample conveying action.

20. The method of claim 17, wherein when the second stepping motor is turned off and the first stepping motor is turned on, the sampling component moves to a specified position in the horizontal direction; and when the first stepping motor is turned off and the second stepping motor is turned on, the sampling needle performs the vertical lifting motion with the transfer guide track under the drive of the second synchronous belt, wherein the specified position comprises the sampling position, the reaction cell position and the counting cell position.

21. (canceled)

22. The method of claim 16, wherein the sampling device further comprises an optocoupler switch, which is installed on the frame; and optocoupler switch sensor chips are respectively installed on the sampling component bracket and the guide track holder block, and when the sensor chips shield the optocoupler, a switching signal is sent to a control system.

23. The method of claim 16, wherein the sampling needle holder block comprises a partial cone-shaped guiding column, and a conical hole matched with the guiding column is formed in a corresponding position of the guide track holder block, and when the sampling component is in contact with the guide track holder block, the guiding column is screwed into the conical hole to keep close contact between the sampling component and the holder block.

24. (canceled)

25. The method of claim 23, wherein when the sampling component moves into contact with the guide track holder block in the horizontal direction, the sampling needle holder block combines with the guide track holder block through the guiding column and the conical hole, and then the sampling needle holder block, together with the guide track holder block, drives the sampling needle to execute tube puncturing and sample aspirating actions under the traction of the second synchronous belt, and after the sample aspirating action is completed, the sampling needle holder block breaks away from the guide track holder block, and then drives the sampling needle to convey the sample in the horizontal direction.

Description

BRIEF DESCRIPTION OF THE DRAWINGS

[0047] FIG. 1 is a planar schematic diagram of a two-dimensional freedom degree motion mechanism of the present invention.

[0048] FIG. 2 is a stereoscopic schematic diagram of a two-dimensional freedom degree motion mechanism of the present invention.

[0049] FIG. 3 is a planar schematic diagram of an open sampling position of another two-dimensional freedom degree motion mechanism of the present invention.

[0050] FIG. 4 is a stereoscopic schematic diagram of an open sampling position of another two-dimensional freedom degree motion mechanism of the present invention.

[0051] FIG. 5 is a planar schematic diagram when another two-dimensional freedom degree motion mechanism of the present invention moves to a specific position in the horizontal direction.

[0052] FIG. 6 is a planar schematic diagram when another two-dimensional freedom degree motion mechanism of the present invention moves to another specific position in the vertical direction.

[0053] FIG. 7 is a planar schematic diagram when another two-dimensional freedom degree motion mechanism of the present invention moves to a puncturing position in the horizontal direction.

[0054] FIG. 8 is a cross-section schematic diagram of a guiding device of another two-dimensional freedom degree motion mechanism of the present invention.

[0055] FIG. 9 is a planar schematic diagram of tube puncturing of another two-dimensional freedom degree motion mechanism of the present invention.

[0056] FIG. 10 is a stereoscopic schematic diagram of a structure of another two-dimensional freedom degree motion mechanism of the present invention.

DETAILED DESCRIPTION OF THE EMBODIMENTS

Example 1

[0057] Referring to FIGS. 1 and 2, a specific embodiment of a sampling device of the present invention is illustrated. The sampling device uses two horizontal guiding shafts 2 and 5 for guiding. A first stepping motor 4 drives a synchronous belt 3 to cause a sampling component to move in the horizontal direction (the sampling component is fixedly connected with the synchronous belt 3 by a sampling component bracket 10). A second stepping motor 1 installed on the sampling component bracket 10 drives a synchronous belt 6 to drive a sampling needle 7 to move in the vertical direction under the guide of two vertical guiding shafts 8 and 9. The two-dimensional freedom degree motion of the sampling needle 7 in the horizontal direction and the vertical direction is conducive to achieving the functions of tube puncturing, sample aspirating, sample conveying and the like in a hematology analyzer device.

Example 2

[0058] FIGS. 3 to 10 show another sampling device having a two-dimensional freedom degree motion mechanism of the present invention. The two-dimensional freedom degree means that the motion mechanism or components therein can respectively move along the directions where two sides of a certain fixed or unfixed included angle are located. The said fixed included angle can be 150 degrees, 120 degrees, 90 degrees, 60 degrees, 30 degrees or any other suitable angle. The movement trajectory of the two-dimensional freedom degree can be a straight line, a smooth curve, a smooth parabola, a broken line or a combination thereof. In a preferred embodiment of the present invention, the two-dimensional freedom degree refers to two straight line directions which are vertical to each other. In a more preferred embodiment, the said two-dimensional freedom degree motion comprises a motion part in the horizontal direction and a motion part in the vertical direction. Specific embodiments of the present invention will be illustrated below by using two-dimensional freedom degree motion in the horizontal direction and the vertical direction as one of the specific embodiments of the present invention. The specific embodiments of two-dimensional freedom degree motions in other angles and in other directions can be obtained by analogy.

[0059] Referring to FIGS. 3, 4 and 5, a horizontal motion device comprises a first stepping motor 116 (the main body is shielded and only a rotating shaft is exposed), a first synchronous belt 109, a first synchronous belt wheel 113, a horizontal guide track 108 and a sampling component 99. The said sampling component 99 comprises a sampling component bracket 112, a second guiding shaft 115 fixed to a bracket 112, a sampling needle holder block 114 that is sleeved on the second guiding shaft 115 and can slide along the second guiding shaft 115 in parallel, and a sampling needle 111 fixed to the sampling needle holder block 114, etc. The first stepping motor 116 is fixed to a sampling device frame 100 by a screw, a bolt or a rivet or the like. A rotating shaft of the first stepping motor 116 synchronously rotates with the first synchronous belt wheel 113. The first synchronous belt 109 is installed on the first synchronous belt wheel 113. Preferably, the outer contour of the first synchronous belt wheel 113 is a gear, and the first synchronous belt 109 is a gear belt matched with the gear. The first synchronous belt 109 is driven by the first synchronous belt wheel 113 to move in the horizontal direction. The sampling component bracket 112 is fixed to the first synchronous belt 109 by a screw and a clamping plate (not shown in the figures) and keeps synchronous motion with the first synchronous belt 109. At least one first sliding block 118 is fixed to the sampling component bracket 112. At least one horizontal guide track 108 is fixed to the sampling device frame 100. The first sliding block 118 is in slide fit with the horizontal guide track 108. In a process where the first stepping motor 116 drives the first synchronous belt 109 to move, the first synchronous belt 109 drives the sampling component bracket 112 (together with the entire sampling component 99) to move along the horizontal guide track 108 in the horizontal direction so as to achieve the motion of the device in the horizontal direction.

[0060] As shown in FIG. 4, a second sliding block 119 in sliding connection with a transfer guide track 101 is fixedly connected with the sampling needle holder block 114 by a screw. The transfer guide track 101 and the horizontal guide track 108 should be parallel to each other, and therefore, when the first synchronous belt 109 drives the sampling component bracket 112 (together with the entire sampling component 99) to move along the horizontal guide track 108 in the horizontal direction, the sampling needle holder block 114 has no displacement in the vertical direction.

[0061] As shown in FIGS. 3 and 4, a vertical motion device comprises a second stepping motor 103, a second synchronous belt 105, a second synchronous belt wheel 106, a guide track holder block 104 and the transfer guide track 101. The second synchronous belt 105 is tensioned by a power output shaft of the second stepping motor 103 and the second synchronous belt wheel 106 and performs synchronous motion with the second stepping motor. Preferably, the second synchronous belt wheel 106 is a gear, and the second synchronous belt 105 is a gear belt matched with the synchronous belt wheel. The transfer guide track 101 is fixed to the second synchronous belt 105 by the guide track holder block 104 and performs synchronous motion with the second synchronous belt 105. The sampling needle holder block 114 is in slide fit with the transfer guide track 101 through the second sliding block 119 thereon, so that the sampling needle holder block can horizontally slide on the transfer guide track 101. The transfer guide track 101, the horizontal guide track 108 and the first synchronous belt 109 are horizontally parallel to each other. A first guiding shaft 107 is fixed to the frame 100, and the guide track holder block 104 is installed on the first guiding shaft 107 and can vertically slide on the first guiding shaft 107. A second guiding shaft 115 is fixed to the sampling component bracket 112, and the sampling needle holder block 114 is installed on the second guiding shaft 115 and can vertically slide on the second guiding shaft 115. The first guiding shaft 107, the second guiding shaft 115 and the second synchronous belt 105 are vertically parallel to each other. When the second stepping motor 103 is started, the second synchronous belt 105 performs synchronous motion with the second stepping motor 103. Since the second synchronous belt 105 is fixedly connected with the guide track holder block 104 and the transfer guide track 101, the transfer guide track 101 is driven by the second stepping motor 103 to move up and down in the vertical direction.

[0062] As shown in FIGS. 3 and 4, due to the requirement for guiding, the first guiding shaft 107, the second guiding shaft 115 and the second synchronous belt 105 need to be vertically placed in parallel. The sampling needle holder block 114 and the guide track holder block 104 have linear bearings (not shown in the figures) installed therein and can vertically slide on the second guiding shaft 115 and the first guiding shaft 107 respectively. The guide track holder block 104 fixes the relative positions of the guide track holder block 104 and the second synchronous belt 105 by a screw and a clamping plate (not shown in the figures). The guide track holder block 104 is provided with a groove 121 (as shown in FIG. 10), and the transfer guide track 101 is received in the groove 121, and the guide track holder block 104 is fixedly connected with the transfer guide track 101 by a screw. As mentioned above, the second sliding block 119 in sliding connection with a transfer guide track 101 is fixedly connected with the sampling needle holder block 114, so that the transfer guide track 101 is simultaneously connected with the guide track holder block 104 and the sampling needle holder block 114. The sampling needle holder block 114 and the guide track holder block 104 have the same or similar structures. When the second synchronous belt 105 is driven by the second stepping motor 103 (fixedly installed on the frame) to move in the vertical direction, the guide track holder block 104 indirectly drives, through the transfer guide track 101, the sampling needle holder block 114 to perform reciprocating motion in the vertical direction under the guide of the first guiding shaft 107 and the second guiding shaft 115. As the sampling needle 111 is fixedly connected with the sampling needle holder block 114, the sampling needle 111 can be driven by the second synchronous belt 105 to perform lifting motion in the vertical direction with the transfer guide track 101 so as to accomplish the operations of aspirating a liquid sample and conveying the liquid sample.

[0063] In summary, when the second stepping motor 103 does not work and the first stepping motor 116 works, the sampling component 99 can move to a specified position in the horizontal direction, for example, a sampling position, a reaction cell position, a counting cell position or the like. When the first stepping motor 116 does not work and the second stepping motor 103 works, the sampling needle 111 can be driven by the second synchronous belt 105 to perform the lifting motion in the vertical direction with the transfer guide track 101. The accurate locating of the motion in the horizontal and vertical direction and the motion in the vertical direction depends on a plurality of sensing optocouplers 102 installed on the frame 100. The working principle of the optocoupler is as follows: when a sensor chip shields the optocoupler, a switching signal is sent to a control system. Therefore, a steel sensor chip (not shown in the figures) is respectively installed on the sampling component bracket 112 and the guide track holder block 104. Referring to FIG. 10, the guide track holder block 104 is provided with the groove 121 matched with the transfer guide track 101, a screw 120 above the groove tightly abuts against the guide track, and two screws on the front face ensure the reliable connection with the guide track. The surface of the transfer guide track 101 is parallel to the horizontal guide track 108. The transfer guide track 101 has two functions: a. applying horizontal guide to the sampling component; and b. transferring a moment to the sampling component in the vertical direction to perform tube puncturing.

[0064] When the liquid sample is aspirated, sometimes the sampling needle needs to puncture a cover of a test tube containing the liquid sample, so certain thrust needs to be applied to the sampling needle. As shown in FIGS. 7 to 10, to achieve an optimal puncturing effect, the sampling component should be close to the guide track holder block 104 as much as possible so as to reduce the moment generated by the transfer guide track 101 as much as possible. To further reduce the pressure on the transfer guide track 101 when the test tube cover is punctured, a guiding column 110 is additionally designed in the present invention. Specifically, a guiding column 110 protrudes from one surface of the sampling needle holder block 114 facing to the transfer guide track 101. The guiding column 110 can be integrally designed with the sampling needle holder block 114 and can also be fixedly connected with the sampling needle holder block 114 by a threaded hole or in other manner. The guiding column 110 can be designed into a cylinder or a cone with a tip portion removed, can also be in any shape with a trapezoidal cross section. The guide track holder block 104 is also provided with a conical hole or a hole 122 in other shape matched with the outline of the guiding column 110 at a corresponding position. When the sampling component is in contact with the guide track holder block 104, the guiding column 110 is inserted in the hole 122 on the holder block 104 and keeps certain close fit. In order to reduce the moment necessary for the tube puncturing, when the sampling component moves into contact with the guide track holder block 104 in the horizontal direction, the sampling needle holder block 114 combines with the guide track holder block 104 mainly through the guiding column 110, and the guide track holder block 104 drives through the guiding column 110 the sampling needle holder block 114 to move downward under the traction of the second synchronous belt 105. The sampling needle holder block 114 further drives the sampling needle 111 to move downward so as to execute actions of puncturing the test tube cover and aspirating the sample. After the sample aspirating, the sampling needle holder block 114 can smoothly break away from the guide track holder block 104 and then drive the sampling needle 111 to convey the sample to the reaction cell position in the horizontal direction.

[0065] The sampling device and the sampling method of the present invention have wide applications. For example, they can be applied to the fields of blood cell analyzers and the like.

[0066] The foregoing contents are further detailed descriptions of the present invention in conjunction with the specific embodiments, but the specific implementations of the present invention cannot be deemed as being only limited to these illustrations. For those of ordinary skill in the art to which the present invention belongs, multiple simple deductions or replacements (for example, the transfer guide track in the embodiment is a linear ball guide track, which can be changed to a roller type linear slide track or the like) can also be made without departing from the concept of the present invention, and these simple deductions or replacements shall be encompassed within the protection scope of the present invention.