DEVICE FOR REAL-TIME IN VIVO MOLECULAR ANALYSIS

Abstract

The invention relates to a biological material molecular analysis device characterized in that it includes:—a laser (34) optionally using an optical parametric oscillator (OPO), configured to emit a wavelength between 2.5 ym and 12 put, said configured laser (34) being intended to ablate said biological material by ejecting charged and/or uncharged particles;—a mass spectrometer (31); and—a probe (S, 10) comprising at least one first analysis fiber (A, 14), connected to the laser (34), and a transfer tube (T, 21), connected to the mass spectrometer (31).

Claims

1. A molecular analysis device for a biological material comprising: a laser (34), optionally assisted with an optical parametric oscillator (OPO), configured for emitting a wavelength comprised between 2.5 μm and 12 μm, said thereby configured laser (34) being intended to ablate said biological material by ejecting charged and/or non-charged particles; a mass spectrometer (31); and a probe (S, 10) including: at least one first analysis fiber (A, 14) connected to the laser (34), and a transfer tube (T, 21) connected to the mass spectrometer (31).

2. The device according to claim 1, characterized in wherein the laser (34), optionally assisted with an optical parametric oscillator (OPO) is configured for emitting a wavelength comprised between 2.5 μm and 3.5 μm.

3. The device according to claim 1, wherein the laser (34), optionally assisted with an optical parametric oscillator (OPO), is configured for emitting a wavelength comprised between 2.8 μm and 3.2 μm.

4. The device according to claim 1, wherein the laser (34) is a pulsed laser configured for generating a beam for which the energy is comprised between 2 mJ/pulse and 15 mJ/pulse and the surface of which is comprised between 30 μm2 and 3 mm2.

5. The device according to claim 1, wherein the laser (34) is a pulsed laser configured for generating a beam for which the energy is comprised between 5 mJ/pulse and 12 mJ/pulse, for example between 5 mJ/pulse and 10 mJ/pulse and the surface is comprised between 30 μm2 and 3 mm2.

6. The device according to claim 4, wherein the laser (34) is a configured for generating a beam for which the surface area is comprised between 0.5 mm2 and 2 mm2.

7. The device according to claim 1, wherein a system (42) for focussing and/or transferring, molecular ions intended to be formed during ablation of biological material, is interposed between said transfer tube (T, 21) and the mass spectrometer (31).

8. The device according to claim 7, wherein said focussing and/or transfer system (42) is a transfer capillary.

9. The device according to claim 1, wherein said transfer tube (T, 21) is provided with a heating means (44).

10. The device according to claim 6, further comprising a de-pressurization capillary (41) inserted between said transfer tube (T, 21) and said focussing and/or transfer system (42), the inner diameter of this de-pressurization capillary (41) being less than that of the transfer tube (T, 21).

11. The device according to claim 10, wherein the de-pressurization capillary (41) is a metal capillary and in that a means is provided for generating a potential difference between said de-pressurization capillary (41) and the mass spectrometer (31).

12. The device according to claim 1, wherein a metal grid (48) is introduced between said transfer tube (T, 21) and said mass spectrometer (31).

13. The device according to claim 10, further comprising an electrospray capillary (45) connected to said de-pressurization capillary (41) and further connected to a means for distributing solvent (46).

14. The device according to claim 1, is no electrospray capillary connected to a means for distributing solvent is provided between the transfer tube (T, 21) and the mass spectrometer (31).

15. The device according to claim 10, further comprising a distribution capillary connected to said de-pressurization capillary and further connected to a gas distributor.

16. The device according to claim 1, is no distribution capillary connected to a gas distributor is provided between the transfer tube (T, 21) and the mass spectrometer (31).

17. The device according to claim 1, wherein said probe (S, 10) includes a second analysis fiber (15).

18. The device according to claim 1, wherein said probe (S, 10) further includes a therapy fiber (16).

19. The device according to claim 18, wherein said therapy fiber (16) is connected to a therapy laser (36).

20. The device according to claim 1, wherein said probe (S, 10) further includes an illumination channel (12) and an image shooting channel (13).

Description

[0039] The present invention will now appear in a more detailed way within the scope of the description which follows of an exemplary embodiment given as an illustration with reference to the appended figures which illustrate:

[0040] FIG. 1 shows a perspective scheme of a probe according to a first embodiment of the invention,

[0041] FIG. 2 shows a perspective scheme of an endoscopic probe according to the invention,

[0042] FIG. 3 shows a scheme of this probe connected to the equipment required for its application,

[0043] FIG. 4 shows a scheme of the transfer line which connects the probe to the mass spectrometer,

[0044] FIG. 5 shows a spectrometry diagram relative to the analysis of a biological tissue ex vivo and notably of a bovine liver, more particularly:

[0045] FIG. 5a shows the total ion current over the whole of the acquisition period,

[0046] FIG. 5b, illustrates the spectrum obtained during the laser irradiation period,

[0047] FIG. 6 shows a comparison of an ex vivo analysis between the liver and the brain of a rat, more particularly,

[0048] FIG. 6a shows the results obtained on the liver,

[0049] FIG. 6b shows the results obtained on the brain,

[0050] FIG. 7 shows an analysis of a cancer biopsy of a lymphoma of a dog

[0051] FIG. 8 shows analyses of digital imprints, a comparison man/woman (in vivo analysis on fingers).

[0052] The elements present in several figures are assigned a single and same reference.

[0053] With reference to FIG. 1, a probe has been illustrated according to the invention in quasi-contact with a biological material to be analyzed P, the skin of a patient in this case (but this may quite also be an organ).

[0054] The probe S appears as a cylinder in which appear an analysis fiber A and a transfer tube T. Both of these elements are flushed with the front face of the probe, the one which comes in proximity to the biological material to be analyzed. The function of these elements is explained later on.

[0055] This is the embodiment at the basis of the invention which gives the possibility of carrying out an external analysis on the skin, the hair, the nails or internally on an organ during open surgery.

[0056] According to a development of the invention, the probe is in fact an endoscopic probe which includes additional elements.

[0057] With reference to FIG. 2, the endoscopic probe 10 appears here as a cylinder having an axial recess 11.

[0058] It has an analysis face or a front face which is visible in the figure and which also has an opposite face, the rear face which does not appear in the figure.

[0059] The actual recess 11 is also cylindrical and a transfer tube 21 is inserted therein. The function of this transfer tube is detailed further on.

[0060] In parallel with the recess 11 are placed several elements which then also are cylindrical.

[0061] First, an illumination channel 12 such as an optical fiber opens onto the rear face so as to be connected to an illumination device not shown in this figure.

[0062] Secondly, an image shooting channel 13 is positioned in proximity to the illumination channel 12. It includes an image shooting apparatus such as a camera and the output on a rear face is accomplished through a video link 23.

[0063] Thirdly, a first optical analysis fiber 14 which is flushed with the analysis face opens onto the rear face. Its connection is explained subsequently.

[0064] Advantageously, a second optical analysis fiber 15 may be provided which also opens onto the rear face.

[0065] For the case when the probe is also used for treatment by laser therapy, the latter further includes a laser therapy fiber 16 which again opens onto the rear face.

[0066] With reference to FIG. 3, the different connections of the probe 10 are explained.

[0067] The illumination channel 12 is connected to an illumination device 32.

[0068] The video link 23 is connected to a viewing screen 33.

[0069] The transfer tube 21 is connected to a mass spectrometer 31. The detail of this connection is provided later on.

[0070] This transfer tube preferably consists of a material aiming at minimizing the absorption phenomena in order to ensure an efficient transfer of the ablated material through the analysis fiber. This material is for example PTFE.

[0071] The first analysis fiber 14 is connected to a first ablation laser 34. This laser 34 has the function of sampling the tissue which it irradiates thereby causing ejection of charged particles (molecular ions) and/or non-charged particles in a gaseous phase.

[0072] The wavelength of this laser may be selected in the domain which extends from infrared to ultraviolet, preferentially in the infrared.

[0073] For example this is a laser with a wavelength comprised between 2.8 μm and 3.2 μm, typically an erbium-YAG laser emitting at a wavelength of 2.94 μm.

[0074] Mention may also be made of: [0075] Neodymium-YAG lasers: 1.064 μm; 0.532 μm; 0.355 μm; 0.266 μm [0076] Xe—Ne lasers: from 2 μm to 4 μm [0077] HF (Hydrogen fluoride) lasers: 2.6 μm [0078] Fiber lasers of the Ytterbium type, doped with bismuth, thulium or holmium: from 1.07 μm to 2.1 μm.

[0079] These lasers may be used as direct sources of emission or coupled with an OPO (Optical Parametric Oscillator). An OPO actually allows from a laser wave of a given wavelength to produce two waves with a larger wavelength. This therefore gives the possibility of widening the range of wavelength which may be used for the relevant laser, seen by the biological material to be ablated.

[0080] Generally, it is sought to cover the range of wavelengths from 2.5 μm to 12 μm. Indeed, in this range of wavelengths, the absorption bands of O—H, N—H, C—H, C═O, C═N, C═C, C—O, C—N and C—C bonds are covered, which may be present in the biological material to be ablated.

[0081] In particular, in this range of wavelengths from 2.5 μm to 12 μm, the laser gives the possibility of ablating the biological material by generating at least charged particles, in particular molecular ions, in a gas phase.

[0082] However it is possible to limit oneself to the range of wavelengths comprised between 2.5 μm and 3.5 μm. Indeed, in this range of wavelengths, the absorption bands of the O—H, N—H and C—H bonds are covered.

[0083] It is possible to limit oneself to a range of more limited wavelength, comprised between 2.8 μm to 3.2 μm. Indeed, in this range of wavelengths, the absorption bands of the O—H and N—H bonds are covered.

[0084] Moreover, the ablation laser 34 will advantageously be a pulsed laser configured for generating a beam for which the energy is advantageously comprised between 2 mJ/pulse and 15 mJ/pulse, the surface of the beam (focussing) being comprised between 30 μm.sup.2 to 3 mm.sup.2.

[0085] The energy of the beam may be comprised between 5 mJ/pulse and 12 mJ/pulse, or further between 5 mJ/pulse and 10 mJ/pulse, the surface area of the beam (focussing) being comprised between 30 μm.sup.2 to 3 mm.sup.2.

[0086] For the ranges of energies considered earlier, the laser beam may moreover have a surface area comprised between 100 μm.sup.2 and 3 mm.sup.2, between 10.sup.−3 mm.sup.2 and 3 mm.sup.2, between 10.sup.−2 mm.sup.2 and 3 mm.sup.2, between 10.sup.−1 mm.sup.2 and 3 mm.sup.2, between 0.5 mm.sup.2 and 3 mm.sup.2, or between 0.5 mm.sup.2 and 2 mm.sup.2. In particular, the beam of the laser may typically ablate a volume of biological material for which the base surface is of the order of the 1 mm.sup.2, which substantially corresponds to a beam for which the surface area or focussing is also of the order of 1 mm.sup.2.

[0087] The inventors have actually been able to ascertain that this selection in the energy provided by a pulse of the ablation laser 34 and in the surface area (focussing) of this beam ensured better production of molecular ions and therefore brought a synergistic effect with the selection of the range of wavelengths of the laser specified above.

[0088] Moreover it should be noted that the penetration depth of the laser beam is typically of a few microns per laser pulse.

[0089] If a second diagnostic fiber is provided, the latter is connected to a second ablation laser of a type different from the first. The possibilities of the diagnostic are thereby increased. Let us mention as an example the Neodymium-YAG laser at a wavelength of 0.532 μm. Let us also mention as an example also another laser, optionally coupled with an OPO for acting in the range from 2.5 μm to 3.5 μm. Said second analysis fiber advantageously giving the possibility of increasing the analysis possibilities.

[0090] The thereby ejected particles are managed by the transfer tube 21 which will forward them to the mass spectrometer 31.

[0091] The laser therapy fiber 16 is connected to a therapy laser 36. Actually, if the analysis carried out earlier reveals that the tissues have to be treated, the treatment may take place immediately, this without using any piece of additional equipment. Thus, for example it is possible to use for therapy, a laser (laser diode) emitting at 980 nm, as proposed by Gonzalez-Mertinez et al., “Robot-assisted stereoactic laser ablation in medically intractable epilepsy: operating technique, Neurosurgery, 2014, suppl 2: 167-172.

[0092] With reference to FIG. 4, a connection example is detailed between the probe 10 and the mass spectrometer 31.

[0093] The transfer tube 21 is extended with a de-pressurization capillary 41 on the opposite side to that of the probe 10. The capillary is preferably a metal capillary. It has a smaller internal diameter than that of the transfer tube 21. This gives the possibility of increasing the de-pressurization of the mass spectrometer 31, which induces an acceleration of the particles. If this capillary 41 is a metal capillary, it is possible to generate an electric field for generating a potential difference between this capillary and the inlet of the mass spectrometer 31.

[0094] The de-pressurization capillary 41 is extended at the inlet of the mass spectrometer 31 with a system for focussing and transferring ions 42 integrated inside the mass spectrometer.

[0095] Alternatively, this system 42 may be positioned outside the mass spectrometer, upstream from the latter, with reference to the path of charged or non-charged particles.

[0096] Moreover, also alternatively, this system 42 may be a system for focalizing ions or a system for transferring ions.

[0097] This system 42 gives the possibility of guiding the aerosol including charged particles or not towards the mass spectrometer.

[0098] The transfer tube 21 may be provided with a heating means 44 for increasing its temperature.

[0099] It is also possible to provide a nebulization capillary 45 which will be connected to the de-pressurization capillary 41. This nebulization capillary 45 is supplied with a distributor of solvent 46. A control member 47 is provided for regulating the distributor 46 so that the solvent flow rate is the desired one. This solvent gives the possibility of reproducing a conventional electrospray process in order to increase the production yield of charged molecules (molecular ions). The advantage of introducing the solvent in this location is the absence of toxicity both towards the users and towards biological tissues.

[0100] However, this is only proposed as an option. Also, it is possible to contemplate that no electrospray means connected to a solvent distribution means is provided between the transfer tube T, 21 and the mass spectrometer 31. Indeed, an advantage of the invention is that the ablation process of the biological material gives the possibility of ejecting at least charged particles (molecular ions) in a sufficient amount of their subsequent analysis with the mass spectrometer.

[0101] Moreover, it is also possible to provide a distribution capillary which will be connected to the de-pressurization capillary. This distribution capillary is then supplied by a gas distributor containing GH+ ions. This gives the possibility of inducing, by collision, a transfer protons to the particles to be analyzed and thereby increase the yield of the ion production. This case is not illustrated in the appended figures, but the implantation of this distribution capillary and of the gas distributor may be similar to that of the electrospray capillary 45 and to its solvent distributor 46, respectively.

[0102] However, this is only proposed as an option. Indeed, it may be contemplated that no distribution capillary connected to a gas distributor is provided between the transfer tube T, 21 and the mass spectrometer 31. Indeed and as a reminder, an advantage of the invention is that the ablation process of the biological material gives the possibility of ejecting at least charged particles (molecular ions) in a sufficient amount for their subsequent analysis by the mass spectrometer.

[0103] It is also possible to provide a metal grid 48 in the transfer line which goes from the probe 10 to the mass spectrometer 31.

[0104] This grid is for example positioned between the transfer tube 21 and the de-pressurization capillary 41, as illustrated in FIG. 4.

[0105] This is an extremely thin grid of the type of those which are used in electron microscopy. Its function is to break the particles or the aggregates of particles ejected by the ablation laser 34 so that they are finally accelerated intended for the mass spectrometer 31. This grid 48 does not break the molecules, some of them are in an ionic form in the transfer tube T, 21, but larger particles.

[0106] A mass spectrometer conventionally comprises and in the following order: [0107] a source [0108] a system for transferring and focussing ions [0109] at least one mass analyzer

[0110] It also comprises a detection system.

[0111] According to a particular embodiment of the invention, the mass spectrometer does not include any source and comprises at least one system for focussing and/or transferring ions interposed between the transfer tube and said mass analyzer.

[0112] In a non-limiting way, mention may be made as examples of system for focussing and/or transferring ions, a transfer capillary, a skimmer, a focussing lens, a transfer system with multipolar fields, an ion funnel, an electrostatic lens.

[0113] The mass spectrometer may also include elements aiming at improving its performances such as for example an ion mobility system.

[0114] According to a possible embodiment, the system for focussing and transferring ions is a transfer capillary.

[0115] The spectrometer comprises a mass analyzer 49. The mass analyzer used may be of any type but should be simple (eg. Simple Magnetic Sector (B) or with double focussing (BE, EB), Quadripole (Q), Ion trap (IT), time of flight (TOF), cyclotronic ion resonance (CIR), orbitrap), combined (eg. Triple Quadripole) or hybrid (eg. Q-orbitrap, Q-TOF).

[0116] Alternatively, the mass analyzer used may be another system for separating ions (e.g. ion mobility).

[0117] Let us now tackle the contemplated strategy upon using the present invention.

[0118] The device according to the invention operates on the basis of a laser ablation process giving the possibility of sampling the biological material to be analyzed. This leads to the ejection of particles in a gas phase (either charged or not). The ablated material is delivered in real time to the mass spectrometer 31 via the transfer tube 21. Indeed, the ablation process and the ejection of particles, related to this ablation, as well as the transit time in the transfer tube are short and may be described as real time. This gives the possibility of collecting the molecular profiles (signals stemming from the analysis of the biological material corresponding to biomolecules of the types of organic compounds, amino acids, metabolites, lipids, peptides, . . . ) characteristic of the analyzed area.

[0119] Advantageously, these profiles will be compared with a data bank of molecular profiles obtained by the use of the present device, in real time allowing information to be obtained in a rapid way.

[0120] The bank of molecular data is established by using the present device in an ex-vivo way on biopsies of patients illustrating different grades and stages of the relevant pathology. A cohort of samples from healthy patients or not is also integrated into the data bank.

[0121] However it is possible to establish the data bank in another way.

[0122] According to a particular use, the surgeon will displace the probe at the surface or at the inside of the patient over the relevant biological material in order to determine whether it is located in a cancer area or not allowing him/her to rapidly contemplate a treatment for the patient and notably the areas which he/she will have to remove surgically. These areas to be removed may advantageously be removed by the therapy fiber according to a particular embodiment of the device according to the invention.

[0123] The invention gave the possibility of obtaining the following results.

Result 1: Analysis of Biological Tissues ex vivo

[0124] A laser emitting nanosecond pulses at a frequency of 10 Hz (Quantel Easy Brillant, Les Ulis, France), connected to an OPO system with a crystal of the LiNbO.sub.3 (variable in wavelength between 2.5 and 4.5 μm, LaserSpec, Malonne, Belgium) adjusted to a wavelength of 2,940 nm is used. A Teflon transfer tube (inner diameter 10 mm) is used for transferring charged and non-charged particles, and is directly connected to a mass spectrometer of the ion trap type for which the source has been withdrawn (HCT Ultra, Bruker Daltonics, Bremen, Germany). The arrival of N.sub.2 of the mass spectrometer was disconnected in order to allow the addition of a pump aiming at increasing the suction flow rate in the transfer tube. The analysis of the compounds stemming from laser irradiation is carried out with the mass spectrometer in a negative mode over a mass over charge (m/z) ratio range comprised between 150 and 1,000.

[0125] The first experiment shown in FIG. 5 is the analysis ex vivo of a piece of bovine liver. On the latter, an irradiation of 7 mJ/laser shot over an area of 1 mm.sup.2 (1 laser shot=1 laser pulse) is achieved. A number of 3 phases was selected during the acquisition step: a first step in absence of laser irradiation, a phase with laser irradiation and another phase in the absence of laser irradiation. FIG. 5A illustrates the total ion current over the whole of the acquisition period and FIG. 5B shows the spectrum obtained during the laser irradiation period. The total ion current shows that the presence of a detected signal is in correlation with laser irradiation. Therefore, there are no compounds which adhere to the internal wall of the transfer tube and this shows a rapid analysis, in real time. Typically, the analysis time is less than 1 second.

[0126] In FIG. 6, a comparison of 2 organs in rat, the brain and the liver is carried out on an acquisition on each organ. An irradiation of 30 seconds at 7 mJ/laser shot over a region of each organ. FIG. 6 shows a m/z ratio difference between these 2 organs which shows a specificity of molecular composition of the brain as compared with the rat liver. In FIG. 7, an analysis of a cancer biopsy of a lymphoma of dogs is carried out. An irradiation of 30 seconds at 7 mJ/laser shot is carried out over a region of this biopsy. The recorded spectrum is selected at the laser irradiation period. It shows a signal generation corresponding to lipids and to fatty acids and a few signal ascribable to peptides. The present invention gives the possibility of carrying out analysis in real time without contamination at the wall of the Teflon tube but also between different organs stemming from a same animal. The real time analysis carried out on the different organs shows a significant number of signals which may correspond to fatty acids, metabolites and lipids. With reference to FIG. 6, the present invention has the capability of detecting different profiles according to the investigated organs and to their physiological status (e.g. healthy versus carcinogenic). The advantage of this analysis is a large disparity in the families of detected molecules which may add a significant value both on the data bank of molecular profiles but also on the characterization of the biological tissues.

Result 2: Analysis of Biological Tissues in vivo

[0127] In FIG. 8, a real time analysis in-vivo of the tissues of the skin of individuals of different gender is carried out at the fingers. An irradiation of 10 seconds at 9 mJ/laser shot (1 laser shot=1 laser pulse) for each individual is carried out by means of a laser emitting nanosecond pulses at a frequency of 10 Hz (Quantel Easy Brillant, Les Ulis, France), connected to an OPO system with a crystal of the LiNbO.sub.3 type (variable in wavelength between 2.5 and 4.5 μm, LaserSpec, Malonne, Belgium) adjusted to a wavelength of 2,940 nm. A Teflon transfer tube (inner diameter 10 mm) is used for transferring either charged and non-charged particles, and is directly connected to a mass spectrometer of the ion trap type for which the source has been withdrawn (HCT Ultra, Bruker Daltonics, Bremen, Germany). The arrival of N.sub.2 of the mass spectrometer was disconnected in order to allow the addition of a pump aiming at increasing the suction flow rate in the transfer tube. The analysis of the compounds stemming from laser irradiation is carried out with the mass spectrometer in a negative mode over a range of mass over charge ratio (m/z) comprised between 150 and 1,000. A number of 3 phases was selected during the acquisition step: a first phase in the absence of laser irradiation, a phase with laser irradiation and another phase in the absence of laser irradiation. A distinction of the different individuals is viewed in FIG. 8. The present invention gives the possibility of carrying out analysis in vivo in real time on individuals and to be able to observe molecular profile specific according to the gender of the individual. This analysis in vivo on individuals shows the non-invasive and painless effect of the present invention during several seconds of irradiation.

[0128] Another advantage of the present invention is the non-invasive effect on organs during the actual irradiation for several tens of seconds on a same point. Based on the molecular profile, the present invention is used for differentiation of very reduced areas (diameter of the irradiation area of 400 μm) of biological tissues.

[0129] The invention also relates to a method for molecular analysis of biological material, characterized in that it comprises the following steps: [0130] emitting a laser beam at a wavelength comprised between 2.5 μm and 12 μm towards a biological material, the interaction between this beam and the biological material causing the ablation of the latter and the ejection of at least charged particles, notably molecular ions; [0131] directing the ablated biological material including at least said charged particles towards a mass spectrometer, via a transfer tube; and [0132] analyzing the composition of the ablated biological material in the mass spectrometer.

[0133] This molecular analysis method of biological material may be carried out in vivo.

[0134] The laser beam may have a wavelength comprised between 2.5 μm and 3.5 μm or between 2.8 μm and 3.2 μm.

[0135] Moreover, the laser beam may be pulsed and have an energy comprised between 2 mJ/pulse and 15 mJ/pulse, the surface of the beam (focussing) being comprised between 30 μm.sup.2 and 3 mm.sup.2.

[0136] The energy of the beam may be comprised between 5 mJ/pulse and 12 mJ/pulse, or further between 5 mJ/pulse and 10 mJ/pulse, the surface of beam (focussing) being comprised between 30 μm.sup.2and 3 mm.sup.2.

[0137] For the ranges of energies considered earlier, the laser beam may moreover have a surface area comprised between 100 μm.sup.2 and 3 mm.sup.2, between 10.sup.−3 mm.sup.2 and 3 mm.sup.2, between 10.sup.−2 mm.sup.2 and 3 mm.sup.2, between 10.sup.−1 mm.sup.2 and 3 mm.sup.2, between 0.5 mm.sup.2 and 3 mm.sup.2, or between 0.5 mm.sup.2 and 2 mm.sup.2. In particular, the beam of the laser typically gives the possibility of ablating a volume of biological material for which the base surface is of the order of the 1 mm.sup.2, which substantially corresponds to a beam also of the order of 1 mm.sup.2.

[0138] Further, this method may provide a step for heating the ablated biological material.

[0139] This method may also provide a step during which the ablated biological material is sifted with a grid, for example a metal grid.

[0140] Finally, it should be noted that the invention also proposes the use of a molecular analysis device according to the invention for ablating said biological material by ejecting charged and/or non-charged particles, and more specially for ablating said biological material by ejecting at least charged particles, notably molecular ions.