A single-use bioreactor is provided. The single-use bioreactor may include a bioprocess container, a shell, at least one agitator, at least one sparger, at least one gas filter inlet port for the sparger(s) and headspace overlay, at least one fill port, at least one harvest port, at least one sample port, and at least one probe. In examples, at least one controller may monitor and control one or more parameters associated with the single-use bioreactor A method to cultivate and propagate mammalian cells is also provided. The method may include cultivating under suitable conditions and in a suitable culture medium in a first single-use bioreactor, transferring the medium containing the cells obtained by propagation from the at least one mammalian cell is into a second single-use bioreactor, transferring the medium containing the cells obtained by propagation from the at least one mammalian cell is into a third single-use bioreactor, and cultivating the cells in the third bioreactor.

Embodiments for coating a bioreactor with a reagent are described. The embodiments may provide for changing flow rates, direction of flow, and/or bioreactor rotation to enhance the coating of the bioreactor prior to growing cells in the bioreactor.

The present disclosure relates to a cell culture apparatus, methods for cell cultivation by using the cell culture apparatus, and a cell culture incubator comprising the cell culture apparatus. The cell culture apparatus comprises a plurality of cell culture modules arranged adjacent to each other. Each cell culture module comprises two or more culture medium reservoirs connected by two or more flow channels. The flow channels go through a basal chamber arranged under an apical chamber. The apical and basal chambers are separated by a porous membrane. The bottom part of the at least two culture medium reservoirs comprise a holding member configured to be compatible with a liquid handling system. The basal chamber has a bottom part arranged higher than a bottom part of each of the culture medium reservoirs. The cell culture apparatus further comprises a cavity under the bottom part of the basal chamber.

A fluid supply interface includes a line component having a first coupling formation for the temporary coupling of a first fluid channel, a second coupling formation for the temporary coupling of a second fluid channel, and a third coupling formation for the temporary or permanent coupling of a third fluid channel, each of said coupling formations being penetrated by a fluid line section, wherein in the line component, a fluid line assembly is formed, by means of which each fluid line section of the first, second and third coupling formations is or can be connected to each fluid line section of the other two coupling formations for the purpose of fluid transport.

To provide a closed system culture vessel for realizing culturing in which an aseptic state is maintained at the time of culturing, and making the reduction of the shear stress generated during conveyance after manufacturing possible. A closed system culture vessel 201 holds a second vessel 302 consisting of an insert vessel on the inside of a first vessel 301, and a lid portion 303 of the closed system culture vessel 201 forms a closed space with the first vessel 301 and has a convex portion 305 contacting the entire outer circumference of the second vessel 302. During conveyance, the closed system culture vessel is conveyed in a state in which the culture medium for conveyance is filled within the second vessel 302.

A cell culture apparatus comprises at least one storage space, at least one barrier, a working space, a sterilization space, and a control space. The at least one storage space comprises a container shelf, an upright track, a rotatable holder, and a light tube. The working space comprises a filling station and an inspecting station. The filling station comprises a first delivering device, an open-close device, and a filling mechanism. The inspecting station comprises a first inspecting mechanism and a second delivering device. Also provided is a method of using the cell culture apparatus including steps of filling, inspecting, or collecting cell cultures, therefore, the cell culture apparatus performs multiple cell culture processes automatically.

A fluid filtration assembly includes a filter housing having first and second ends and a connector for fluid communication with a fluid storage vessel. A filter element is disposable within the filter housing, and first and second pumps are coupled at the first and second ends of the filter housing. A controller may coordinate the operation of the first and second pumps to induce alternating tangential flow of fluid between the filter housing and the first and second pumps. At least one of the first and second pumps is a diaphragm pump or a plunger pump. The fluid storage vessel can be a bioreactor.

The invention relates to devices, methods and systems for collecting and solidifying waste material from bioreactor cell cultures in order to facilitate waste disposal. The invention finds particular utility in mammalian cell culture applications. A transverse wall divides the interior of a waste bag into two chambers, the first chamber containing an inlet receiving waste liquid from a bioreactor, the second chamber containing an absorbent material to solidify the liquid into a gel. The transverse wall acts to direct the flow of waste media into the second chamber where it is converted into a gel and further prevents the inlet from being blocked by any gel or particulate materials. Methods and uses regarding this invention are described.

A bioprocess vessel includes a flexible bag or substantially rigid container that defines an interior volume and having a bottom surface, the bottom surface being open or containing an aperture therein for the passage of fluid. A pump is secured to the bottom surface of the flexible bag or substantially rigid container. In some embodiments is secured directly to the flexible bag or substantially rigid container. In other embodiments, the pump is secured indirectly the flexible bag or substantially rigid container using, for example, a port that extends through the aperture on the bottom surface. The port or flanged surface may also be integrated into the pump, which is secured to the vessel. An optional mixing adaptor may be provided inside the interior volume of the flexible bag or substantially rigid container and at least partially covers the inlet that leads to the pump.

Provided is a cell-culturing apparatus including a culturing unit in which a culturing vessel is placed and an incubator in which a plurality of culturing units are placed in an interior thereof, and that can maintain the interior thereof at an environment that is appropriate for cell culturing, wherein the incubator includes openings that are provided for each of the plurality of culturing units and through which the culturing units can separately be moved into and out of the incubator, and closing means that are provided at the openings and that seals off the internal environment of the incubator from outside when the culturing unit is taken out of the incubator.