Systems and methods are provided for labelling nucleic acids in a cell of a cell culture or of a tissue. The systems and methods include contacting the cell culture or tissue with a first label and applying an electrical field to the cell, thereby increasing the permeability of the cell membrane, allowing the first label to be introduced into the cell. The cell culture or tissue can be disposed on a multielectrode array, and the electrical field can be applied by operating one or more electrodes of the array that are proximate to (e.g., beneath) the cell in order to control the location of the cell whose permeability is increased and/or to control the timing of such permeability increase.

The present invention relates to the field of stem cells. More specifically, the present invention provides compositions and methods for using optogenetics to sustain the pluripotency of stem cells. In one embodiment, a vector comprises a nucleotide sequencing encoding a fusion protein comprising the intracellular domain of fibroblast growth factor 1 receptor (FGFR1) and a photoactivatable domain.

The present invention relates to the field of stem cells. More specifically, the present invention provides compositions and methods for using optogenetics to sustain the pluripotency of stem cells. In one embodiment, a vector comprises a nucleotide sequencing encoding a fusion protein comprising the intracellular domain of fibroblast growth factor 1 receptor (FGFR1) and a photoactivatable domain.

Aspects of the disclosure are directed to a technique for sequential electroporation that provides for a delivery of multiple electrical pulses separated in time to cells, cell particles, lipid vesicles, liposomes, or to increase efficiency of entry of one or more agents of interest into cells, cell particles, lipid vesicles, liposomes, tissues, or derivatives thereof, and to minimize damage by electrical arc or heat shock; increase loading efficiency of an agent of interest; and maintain viability of the cells, cell particles, lipid vesicles, or tissues and the ability of the cells, cell particles, lipid vesicles, liposomes, or tissues to produce a clinical effect.

Aspects of the disclosure are directed to a technique for sequential electroporation that provides for a delivery of multiple electrical pulses separated in time to cells, cell particles, lipid vesicles, liposomes, or to increase efficiency of entry of one or more agents of interest into cells, cell particles, lipid vesicles, liposomes, tissues, or derivatives thereof, and to minimize damage by electrical arc or heat shock; increase loading efficiency of an agent of interest; and maintain viability of the cells, cell particles, lipid vesicles, or tissues and the ability of the cells, cell particles, lipid vesicles, liposomes, or tissues to produce a clinical effect.

A disposable, fabrication-free, high-volume electroporation device may process cell samples of large volume without compromising transformation efficiency and cell viability, while precipitously reducing the entire processing time and effort. An embodiment includes at least two hollow, tubular conductive elements and an insulating structure, defining a channel, that fluidically couples the at least two conductive elements to define an electroporation flow path in the channel for flow-through electroporation. The high-volume electroporation device can be an alternative to cuvettes for typical volume electroporation, but can also be an indispensable tool to process large volume samples for applications, such as creation of modified sample libraries.

A disposable, fabrication-free, high-volume electroporation device may process cell samples of large volume without compromising transformation efficiency and cell viability, while precipitously reducing the entire processing time and effort. An embodiment includes at least two hollow, tubular conductive elements and an insulating structure, defining a channel, that fluidically couples the at least two conductive elements to define an electroporation flow path in the channel for flow-through electroporation. The high-volume electroporation device can be an alternative to cuvettes for typical volume electroporation, but can also be an indispensable tool to process large volume samples for applications, such as creation of modified sample libraries.

Methods for characterizing mechanical properties of cells at different stress levels. The disclosed inventions can determine the impact of shear stress on cells in bioproduction processes.

According to the present disclosure, there is provided a cell stimulation and culture platform using a ultrasonic hologram, including a culture vessel in which a culture well with an open lower portion is formed; a transmission sheet which is installed to cover a lower surface of the culture vessel and where a biological sample is seated; a platform body that is filled with a liquid medium and an open upper end is covered by the transmission sheet; an ultrasonic transducer that is installed inside the platform body in a state of being spaced apart from the biological sample; and an ultrasonic hologram lens that is installed on the ultrasonic transducer, and spatially modulates the phase of the ultrasonic waves using a surface structure designed to have different height distributions to focus the ultrasonic waves in a set pattern shape on a target surface on which the biological sample is located.

According to the present disclosure, there is provided a cell stimulation and culture platform using a ultrasonic hologram, including a culture vessel in which a culture well with an open lower portion is formed; a transmission sheet which is installed to cover a lower surface of the culture vessel and where a biological sample is seated; a platform body that is filled with a liquid medium and an open upper end is covered by the transmission sheet; an ultrasonic transducer that is installed inside the platform body in a state of being spaced apart from the biological sample; and an ultrasonic hologram lens that is installed on the ultrasonic transducer, and spatially modulates the phase of the ultrasonic waves using a surface structure designed to have different height distributions to focus the ultrasonic waves in a set pattern shape on a target surface on which the biological sample is located.