The present invention relates to a method for preparing fructose or xylulose from biomass comprising glucose or xylose, and a method for separating a mixture of glucose and fructose and a mixture of xylose and xylulose.

A method for purifying a mixture in a multicolumn chromatography system. The method successively and cyclically collects a raffinate, injects the mixture to be separated, collects an extract, and injects eluent. The mixture to be separated contains fructose and has a dry matter mass concentration of 45 to 55%. The method is carried out at a temperature of 50 to 62° C.

A method for purifying a mixture in a multicolumn chromatography system. The method successively and cyclically collects a raffinate, injects the mixture to be separated, collects an extract, and injects eluent. The mixture to be separated contains fructose and has a dry matter mass concentration of 45 to 55%. The method is carried out at a temperature of 50 to 62° C.

Described herein is a method of adjusting the composition of a chromatography product to achieve a target enriched proportion of a desired component from an input feed having a lower proportion of the desired component using simulated moving bed (“SMB”) chromatography wherein the eluent for the SMB apparatus may comprise the very input feed being enriched. The method is exemplified by enriching a high fructose corn syrup from a 42% fructose syrup to a 55% fructose syrup without substantially reducing the dissolved solids concentration of the 55% syrup relative to the input 42% syrup. The 42% syrup is also used as the eluent for the SMB apparatus and may be reconstituted from the raffinate stream by passing the raffinate stream over a glucose isomerase column alone or in combination with a dextrose feed. The method reduces water usage and saves energy by minimizing the need for evaporation to obtain a 55% fructose syrup with a high dissolved solids content.

Described herein is a method of adjusting the composition of a chromatography product to achieve a target enriched proportion of a desired component from an input feed having a lower proportion of the desired component using simulated moving bed (“SMB”) chromatography wherein the eluent for the SMB apparatus may comprise the very input feed being enriched. The method is exemplified by enriching a high fructose corn syrup from a 42% fructose syrup to a 55% fructose syrup without substantially reducing the dissolved solids concentration of the 55% syrup relative to the input 42% syrup. The 42% syrup is also used as the eluent for the SMB apparatus and may be reconstituted from the raffinate stream by passing the raffinate stream over a glucose isomerase column alone or in combination with a dextrose feed. The method reduces water usage and saves energy by minimizing the need for evaporation to obtain a 55% fructose syrup with a high dissolved solids content.

A method of processing an aqueous solution, wherein the aqueous solution comprises one or more dissolved sugar, one or more dissolved sugar alcohol, or a mixture thereof, wherein the method comprises bringing the aqueous solution into contact with a collection of resin beads, wherein the resin beads comprise functional groups of structure (S1).

The present invention relates to a production process, composition and use of an artificial honey based on the use of inverted sugar, preferably using the enzyme invertase from yeast, with the addition of nutritive components such that the final syrup has a formulation similar to that of natural honey and can have improved pharmaceutical functions and biological functions, being healthier. The artificial honey of the present invention relates to a product of strictly plant origin, which is produced without the use of animal work or biological materials, and can be commercialized in various sectors, including the vegan sector. More specifically, the artificial honey of the present invention is produced from inverted sucrose, preferably by means of the enzymatic catalysis of VHP/VVHP/demerara brown sugars rich in minerals, vitamins and natural antioxidants of great pharmacological interest and various types of substances of interest. The artificial honey of the present invention has no chemical substances that are harmful to human health (e.g. pigments, preservatives or other chemical additives) added to it or produced in it, but can be flavored and/or aromatized with artificial honey flavoring, flavoring identical to natural honey and, optionally, natural honey flavoring, and may also contain fiber, vitamin supplements, minerals, vitamins, amino acids, natural extracts, being optionally directly consumed or used for manufacturing other food products.

The present invention relates to a production process, composition and use of an artificial honey based on the use of inverted sugar, preferably using the enzyme invertase from yeast, with the addition of nutritive components such that the final syrup has a formulation similar to that of natural honey and can have improved pharmaceutical functions and biological functions, being healthier. The artificial honey of the present invention relates to a product of strictly plant origin, which is produced without the use of animal work or biological materials, and can be commercialized in various sectors, including the vegan sector. More specifically, the artificial honey of the present invention is produced from inverted sucrose, preferably by means of the enzymatic catalysis of VHP/VVHP/demerara brown sugars rich in minerals, vitamins and natural antioxidants of great pharmacological interest and various types of substances of interest. The artificial honey of the present invention has no chemical substances that are harmful to human health (e.g. pigments, preservatives or other chemical additives) added to it or produced in it, but can be flavored and/or aromatized with artificial honey flavoring, flavoring identical to natural honey and, optionally, natural honey flavoring, and may also contain fiber, vitamin supplements, minerals, vitamins, amino acids, natural extracts, being optionally directly consumed or used for manufacturing other food products.

Systems comprising a first polynucleotide comprising a regulatory element operatively linked to an open reading frame encoding an invertase enzyme and a second polynucleotide comprising a repressible regulatory element operatively linked to an open reading frame encoding a glucose oxidase enzyme wherein the repressible element inhibits transcription in response to hydrogen peroxide are provided. Cells and compositions comprising a system of the invention, as well as methods using the system of the invention, are also provided, as is artificial honey.

Systems comprising a first polynucleotide comprising a regulatory element operatively linked to an open reading frame encoding an invertase enzyme and a second polynucleotide comprising a repressible regulatory element operatively linked to an open reading frame encoding a glucose oxidase enzyme wherein the repressible element inhibits transcription in response to hydrogen peroxide are provided. Cells and compositions comprising a system of the invention, as well as methods using the system of the invention, are also provided, as is artificial honey.