The present invention relates to a method for improving the production of a difficult-to-express recombinant protein in a recombinant microorganism, and more particularly to a method for improving the production of a difficult-to-express recombinant protein by use of a recombinant microorganism into which a gene encoding a target protein and an sRNA against a gene encoding ribonuclease P are introduced. According to the present invention, expressions of a large recombinant protein, a difficult-to-express protein and a useful protein can be dramatically increased by reducing expression of the rnpA gene.

A composition for detecting more than one target nucleic acid sequence in a nucleic acid sample by polymerase chain reaction (PCR) is provided. Also provided is a kit comprising the above composition with additional reagents for performing PCR using the plasmid, dye, primers and probes to detect the more than one target nucleic acid sequence. Additionally provided is a method for detecting more than one target nucleic acid sequence in a nucleic acid sample by polymerase chain reaction (PCR).

The disclosure relates to a method for increasing the resistance of a plant to a plant RNA virus, comprising expressing in said plant a mutant protein-only RNase P enzyme lacking a nuclear localization signal domain or an organelle targeting sequence domain, and related compositions.

The present invention relates to analysis of an RNA molecule. It further relates to the use of this method for the quality control of an RNA molecule produced by in vitro transcription or for the quality control of an RNA molecule produced by chemical synthesis.

The present invention relates to a method for improving the production of a difficult-to-express recombinant protein in a recombinant microorganism, and more particularly to a method for improving the production of a difficult-to-express recombinant protein by use of a recombinant microorganism into which a gene encoding a target protein and an sRNA against a gene encoding ribonuclease P are introduced. According to the present invention, expressions of a large recombinant protein, a difficult-to-express protein and a useful protein can be dramatically increased by reducing expression of the rnpA gene.

The present invention relates to analysis of an RNA molecule. It further relates to the use of this method for the quality control of an RNA molecule produced by in vitro transcription or for the quality control of an RNA molecule produced by chemical synthesis.

A method is described for improving the production of a difficult-to-express recombinant protein in a recombinant microorganism, and more particularly a method for improving the production of a difficult-to-express recombinant protein by use of a recombinant microorganism into which a gene encoding a target protein and an sRNA against a gene encoding ribonuclease P are introduced. By the disclosed method, expressions of a large recombinant protein, a difficult-to-express protein and a useful protein can be dramatically increased by reducing expression of the rnpA gene.

The present disclosure is directed to polycistronic guide RNAs, DNA encoding polycistronic gRNA, multiplex CRISPR vectors, a plurality of component DNA fragments for assembly into a DNA encoding a polycistronic gRNA array, a plurality of primer pairs for making a plurality of component DNA fragments to be assembled into a DNA encoding a polycistronic gRNA, and methods of making multiplex CRISPR vectors. The current disclosure is directed to multiplexed CRISPR technologies that have great potential for pathway engineering and genome editing. In the current disclosure describes efficient assembly of tRNA/Csy4/Ribozyme-based gRNA arrays which can be produced in a quick and effective process.

The present disclosure relates to the field of molecular biology and immunology. More specifically. the present disclosure provides compositions and methods for detecting anti-Th/To autoantibodies in the serum of subject with a systemic autoimmune disease, such as systemic sclerosis (SSc).

The present disclosure relates to the field of molecular biology and immunology. More specifically, the present disclosure provides compositions and methods for detecting anti-Th/To autoantibodies in the serum of subject with a systemic autoimmune disease, such as systemic sclerosis (SSc).