The present invention discloses an E. coli engineering bacteria producing 1,5pentanediamine through a whole cell catalysis and its application. The engineering bacteria according to the present invention, is Escherichia coli (E. coli) strain B or its derivative strains with the overexpression of a lysine decarboxylase gene and a proper expression of a lysinecadaverine antiporter gene cadB. The engineering bacteria according to the present invention is the engineering bacteria producing 1,5pentanediamine through the whole cell catalysis constructed from Escherichia coli B derivative strains, which has an overexpression of a lysine decarboxylase gene cadA and a proper expression of the lysinecadaverine antiporter gene cadB; The present invention further discloses a method of producing a 1,5pentanediamine catalyzed by the engineering bacteria, the yield and production intensity of 1,5pentanediamine in biobased production could be significantly improved through the method, hence it could be applied to mass production and convenient for extending applications.

The present application relates to a variant of ornithine decarboxylase or protein, a polynucleotide encoding the same, a microorganism containing the same, and a method for producing putrescine using the same. The present invention achieves effects of increasing putrescine productivity, production efficiency or production selectivity, suppressing side reactions, and saving the cost involved in purifying putrescine.

The present invention relates to: a novel lysine decarboxylase; a microorganism transformed with a gene coding for the activity concerned; and a method for producing cadaverine by using the same.

The expression plasmid vectors comprise a polynucleotide sequence encoding Ldc2 polypeptide, a fragment, and/or a mutant. A backbone plasmid is capable of autonomous replication in a host cell. The host cell is not a P. aeruginosa cell. Transformants are transformed with expression plasmid vector. The transformants are not P. aeruginosa. Mutant host cells comprise a polynucleotide sequence encoding Ldc2 polypeptide, a fragment and/or a mutant that has been integrated into the host cell chromosome. A polypeptide, a fragment and/or a mutant comprise Ldc2. A non-naturally occurring polynucleotide, and/or a mutant encodes polypeptide comprising Ldc2. Biobased cadaverine is produced using the transformants and the biobased cadaverine is prepared by the method. Polyamides are formed using the biobased cadaverine and compositions.

The present invention relates to 25 hitherto undescribed genes of B. licheniformis and gene products derived thereform and all sufficiently homologous nucleic acids and proteins thereof. They occur in five different metabolic pathways for the formation of odorous substances. The metabolic pathways in question are for the synthesis of: 1) isovalerian acid (as part of the catabolism of leucine), 2) 2-methylbutyric acid and/or isobutyric acid (as part of the catabolism of valine and/or isoleucine), 3) butanol and/or butyric acid (as part of the metabolism of butyric acid), 4) propyl acid (as part of the metabolism of propionate) and/or 5) cadaverine and/or putrescine (as parts of the catabolism of lysine and/or arginine). The identification of these genes allows biotechnological production methods to be developed that are improved to the extent that, to assist these nucleic acids, the formation of the odorous substances synthesised via these metabolic pathways can be reduced by deactivating the corresponding genes in the micro-organism used for the biotechnological production. In addition, these gene products are thus available for preparing reactions or for methods according to their respective biochemical properties.

The present invention relates to a recombinant microbial cell for producing N5-aminopentyl-N-(hydroxy)-succinamic acid of Formula I from at least one simple carbon source:

##STR00001## where n is 2 and
wherein the simple carbon source is selected from the group consisting of glucose, sucrose, xylose, arabinose, mannose, glycerol and combinations thereof and wherein the cell comprises a further genetic modification to increase production of L-lysine from at least one of the simple carbon sources.

This invention provides acid decarboxylase-prion subunit fusion polypeptides, nucleic acid sequences, expression vectors, and host cells expression such fusion polypeptides to produce various amino acids and derivatives of the amino acids such as polyamines.

The present invention relates to a recombinant microbial cell for producing at least one compound having structural Formula III from at least one simple carbon source:

##STR00001## wherein the simple carbon source is selected from the group consisting of glucose, sucrose, xylose, arabinose, mannose and glycerol; and wherein the cell comprises a further genetic modification to increase production of L-lysine in the cell from at least one of the simple carbon sources.

A recombinant microbial cell is capable of producing at least one compound having structural Formula II from a carbon source:

##STR00001##

In Formula II, n=2-3; R.sub.1=H or COCH.sub.3 or CH.sub.2CH.sub.2COX with X=OH or O; R.sub.2=CH.sub.3 or CH.sub.2CH.sub.2COX with X=OH or O where the cell comprises a genetic modification to increase activity relative to its wild-type cell of E.sub.4 where E.sub.4 is a desferrioxamine or bisucaberin synthetase (EC 6.3.-.-) (E.sub.4i) capable of converting N5-aminopentyl-N-(hydroxy)-succinamic acid to desferrioxamine B or H or at least one other linear desferrioxamine or bisucaberin according to Formula II.

The present invention provides engineered lysine decarboxylases that can be used to synthesize 1,5-diaminopentane under industrially relevant conditions. The present invention also provides polynucleotides encoding engineered lysine decarboxylases, host cells capable of expressing the engineered lysine decarboxylases, and methods for preparing 1,5-diaminopentane using the engineered lysine decarboxylases. The engineered lysine decarboxylase of the present invention was developed from a wild-type lysine decarboxylase through a creative process of directed evolution, and the engineered lysine decarboxylase of the present invention has a better activity and/or stability and tolerates a high substrate concentration compared to other lysine decarboxylases for the preparation of 1,5-diaminopentane, and thus has a good prospect for industrial application.