C12Y402/0103

FERMENTATION PROCESS TO PRODUCE BIOACROLEIN AND BIOACRYLIC ACID
20230357806 · 2023-11-09 ·

A method for producing bioacrolein using renewable glycerol as a feedstock as well as a method for producing bioacrylic acid using bioacrolein as a feedstock are provided in the present invention. Also provided in the present invention are recombinant microbial cells useful in producing 3-hydroxypropionaldhyde from glycerol, method of converting the 3-hydroxypropionaldhyde into bioacrolein and a process for recovering acrolein using a fractional distillation process.

METHOD FOR PRODUCING 3-HYDROXYPROPIONIC ACID
20230340547 · 2023-10-26 ·

Provided is a two-step production method for 3-HP, comprising: a first step of culturing cells at a high concentration; and a second step of producing 3-HP using the high concentration-cultured cells as a catalyst, in which during the two-step culture, the energy and/or coenzyme balance are adjusted to produce 3-HP and/or improve the productivity of 3-HP. The productivity and yield of 3-HP can be improved.

Glycerol free ethanol production

The invention relates to a recombinant yeast comprising a recombinant yeast comprising a nucleotide sequence coding for a glycerol dehydrogenase, a nucleotide sequence coding for a ribulose-1,5-biphosphate carboxylase oxygenase (EC 4.1.1.39); a nucleotide sequence coding for a phosphoribulokinasey (EC 2.7.1.19); a nucleotide sequence allowing the expression of a glucoamylase (EC 3.2.1.20 or 3.2.1.3); and optionally a nucleotide sequence coding for a glycerol transporter. This cell can be used for the production of ethanol and advantageously produces little or no glycerol.

POLY(3-HYDROXYPROPIONATE-B-LACTATE) BLOCK COPOLYMER USING MICROORGANISMS

The present invention relates to a novel 3-hydroxypropionate-lactate block copolymer [P(3HP-b-LA)], and a method for preparing same, and more specifically, provides a method for preparing a 3-hydroxypropionate-lactate block copolymer, and a 3-hydroxypropionate-lactate block copolymer produced thereby, the method comprising: a first culture step in which, by using recombinant E. coli improved so as to be incapable of biosynthesizing lactic acid, P(3HP) is biosynthesized at the early stage of culturing by having glycerol as a carbon source and through 3-hydroxypropionate-generating genes and an enhanced PHA synthase; and a second culture step in which P(3HP) production is inhibited by using a carbon catabolic repression system for selectively introducing only glucose into E. coli when glycerol and glucose are supplied together as carbon sources, and in which polylactate is biosynthesized to an interrupted P(3HP) terminus by the enabling of the expression of a lactate synthase and a lactyl-CoA converting enzyme through an IPTG induction system.

Yeast cells for glycerol free ethanol production

The invention relates to a recombinant yeast comprising a nucleotide sequence allowing the expression of a glucoamylasey (EC 3.2.1.20 or 3.2.1.3). This cell can be used for the production of ethanol and advantageously produces little or no glycerol.

Poly(3-hydroxypropionate-b-lactate) block copolymer using microorganisms

Provided are a novel 3-hydroxypropionate-lactate block copolymer [P(3HP-b-LA)], and a method for preparing same, comprising: a) transforming a recombinant microorganism modified to be incapable of biosynthesizing lactic acid with a vector including a 3-hydroxypropionyl-CoA biosynthesis gene and a polyhydroxyalkanoate (PHA) synthetase gene, and a vector including a lactate biosynthesis gene and a gene of an enzyme that converts lactate to lactyl-CoA; (b) synthesizing poly(3-hydroxypropionate) (P(3HP)) by culturing the recombinant microorganism using a glycerol as a carbon source; and (c) inhibiting P(3HP) production by adding IPTG and glucose, and biosynthesizing polylactate (PLA) at the end of P(3HP) synthesized in step (b) by enabling the expression of a lactate biosynthesis enzyme and an enzyme that converts lactate to lactyl-CoA. Also provided is a recombinant microorganism produced in step a).

Poly(3-hydroxypropionate-b-lactate) block copolymer using microorganisms

The present invention relates to a novel 3-hydroxypropionate-lactate block copolymer [P(3HP-b-LA)], and a method for preparing same, and more specifically, provides a method for preparing a 3-hydroxypropionate-lactate block copolymer, and a 3-hydroxypropionate-lactate block copolymer produced thereby, the method comprising: a first culture step in which, by using recombinant E. coli improved so as to be incapable of biosynthesizing lactic acid, P(3HP) is biosynthesized at the early stage of culturing by having glycerol as a carbon source and through 3-hydroxypropionate-generating genes and an enhanced PHA synthase; and a second culture step in which P(3HP) production is inhibited by using a carbon catabolic repression system for selectively introducing only glucose into E. coli when glycerol and glucose are supplied together as carbon sources, and in which polylactate is biosynthesized to an interrupted P(3HP) terminus by the enabling of the expression of a lactate synthase and a lactyl-CoA converting enzyme through an IPTG induction system.

VARIANT MICROORGANISM HAVING ABILITY TO PRODUCE 1,3-PROPANEDIOL, AND METHOD FOR PREPARING 1,3-PDO BY USING SAME

The present disclosure relates to a mutant microorganism in which a glycerol catabolic pathway and a 1,3-PDO biosynthetic pathway are introduced into a microorganism incapable of using glycerol as a carbon source, and a method of producing 1,3-PDO using the same. According to the present disclosure, it is possible to produce 1,3-PDO while growing a mutant microorganism having 1,3-PDO production ability by using the inexpensive raw material glycerol as a single carbon source. Thus, the present disclosure is useful for the economical production of 1,3-PDO.

RECOMBINANT CORYNEBACTERIUM HAVING 1,3-PDO PRODUCTIVITY AND REDUCED 3-HP PRODUCTIVITY, AND METHOD FOR PRODUCING 1,3-PDO BY USING SAME

The present invention relates to recombinant Corynebacterium having 1,3-PDO productivity and reduced 3-HP productivity, and a method for producing 1,3-PDO by using same. When a Corynebacterium glutamicum variant according to the present invention is used, the productivity of 3-HP, which is a by-product, is inhibited by using low-cost glycerol as a carbon source, and thus 1,3-PDO can be produced with high efficiency.

IMPROVED GLYCEROL FREE ETHANOL PRODUCTION

The invention relates to a recombinant yeast comprising a nucleotide sequence allowing the expression of a glucoamylasey (EC 3.2.1.20 or 3.2.1.3). This cell can be used for the production of ethanol and advantageously produces little or no glycerol.