The present invention relates to a composition comprising one or more novel Streptococcus thermophilus strain(s), and the use of said composition for producing a fermented product such as a dairy product with e.g. an increased sweetness. The invention also relates to novel Streptococcus thermophilus strain(s) as such.

Provided are a Bacillus subtilis genetically engineered bacterium for producing tagatose and a method for preparing tagatose. The genetically engineered bacterium comprises constructing thermostable ?-glucan phosphorylases, thermostable glucose phosphomutases, thermostable glucose phosphate isomerases, thermostable 6-tagatose phosphate epimerases, and thermostable 6-tagatose phosphate phosphatases which are independently expressed or co-expressed. The usage of the genetically engineered bacterium can effectively convert starch into tagatose. Compared with existing methods for producing tagatose, the method has advantages such as suitability for whole-cell recycling, high safety, high yield, simple production process, low cost, and easiness in large-scale preparation.

Disclosed herein, are compositions and methods useful in expressing a functional PGM1 protein in a subject by administration of a recombinant adeno-associated virus vector containing a transgene encoding PGM1. Also disclosed herein are methods for treating a PGM1 gene deficiency in a subject in need thereof.

Provided herein, in some embodiments, are systems, methods, and compositions (e.g., cells and cell lysates) for enzymatically converting a polymeric glucose carbohydrate (e.g., starch) to sugar.

The present invention relates to genetically engineered organisms, especially microorganisms such as bacteria and yeasts, for the production of added value bio-products such as specialty saccharide, activated saccharide, nucleoside, glycoside, glycolipid or glycoprotein. More specifically, the present invention relates to host cells that are metabolically engineered so that they can produce said valuable specialty products in large quantities and at a high rate by bypassing classical technical problems that occur in biocatalytical or fermentative production processes.

The invention relates to preparation of food ingredients enriched with a low-glycemic sugar replacement through enzymatic conversion. Food ingredients may be enriched with, for example, D-tagatose, D-allulose, D-allose, D-mannose, D-talose, and/or inositol by enzymatically converting saccharides found in flour, meal, ground tuber, ground pulse, ground bark, starch, malted grain or malt extract, maltodextrin, cellulose, cellodextrin, any of their derivatives (e.g., amylose, amylopectin, dextrin, cellobiose, etc.), and/or sucrose into D-tagatose, D-allulose, D-allose, D-mannose, D-talose and/or inositol. The enriched material can be used as a food ingredient instead of the low-glycemic sugar being purified for use as a food ingredient.

A method for preparing immobilized cells for producing mannose, and a method using same for producing mannose, comprising: fermenting to separately obtain fermentation broths of Escherichia coli or Bacillus subtilis expressing a-glucan phosphorylase, phosphoglucomutase, glucose phosphoisomerase, mannose-6-phosphate isomerase and mannose-6-phosphate phosphatase, and mixing the fermentation broths to obtain a mixed fermentation broth.

A method for preparing glucosamine includes the steps of converting fructose-6-phosphate (F6P) and an ammonium salt to glucosamine-6-phosphate (GlcN6P) under the catalysis of glucosamine-6-phosphate deaminase (EC 3.5.99.6, GlmD); and producing glucosamine (GlcN) by the dephosphorylation of GlcN6P under the catalysis of an enzyme capable of catalyzing the dephosphorylation. Such a method can be used to prepare glucosamine by in vitro enzymatic biosystem.

Provided herein, in some embodiments, are systems, methods, and compositions (e.g., cells and cell lysates) for enzymatically converting a polymeric glucose carbohydrate (e.g., starch) to sugar.

Disclosed herein are methods of producing hexoses from saccharides by enzymatic processes. The methods utilize fructose 6-phosphate and at least one enzymatic step to convert it to a hexose.