A system for visualizing melanin present in tissue can include an imaging system to record a signal based on a presence of melanin in tissue and a display device to display an image based on the signal. A first laser source can emit a Stokes pulse train and a second laser source can emit a pump pulse train. Both the first laser source and the second laser source comprise a tunable center wavelength or frequency. An energy difference between a frequency of the Stokes pulse train and a frequency of the pump pulse train is from 1750 cm.sup.−1 to 2250 cm.sup.−1. The Stokes and the pump pulse train overlap in space and time. A scanning mechanism focuses the combined Stokes pulse train and pump pulse train within the tissue and scans across the tissue. A detector detects the signal based on a presence of melanin within the tissue.

Current glucose meters provide instantaneous results however are invasive and painful thus causing reduced compliance. A non-invasive, portable, wearable device would be ideal for monitoring and recording and provide a distinct advantage to current glucose monitors.

A method of assessing tissue health comprises the steps of obtaining depth-resolved spectra of a selected area of in vivo tissue, and assessing the health of the selected area based on the depth-resolved structural information of the scatterers. Obtaining depth-resolved spectra of the selected area comprises directing a sample beam towards the selected area at an angle, and receiving an angle-resolved scattered sample beam. The angle-resolved scattered sample beam is cross-correlated with the reference beam to produce an angle-resolved cross-correlated signal about the selected area, which is spectrally dispersed to yield an angle-resolved, spectrally-resolved cross-correlation profile having depth-resolved information about the selected area. The angle-resolved, spectrally-resolved cross-correlation profile is processed to obtain depth-resolved information about scatterers in the selected area.

An optical sensor is provided. The optical sensor has an emitting system including at least one light emitting device which emits light onto an object; and a detecting system detecting the light which has been emitted by the emitting system and which has propagated through the object. The light emitting device is capable of emitting a plurality of light beams with different wavelengths onto substantially the same position of the object.

Infrared imaging devices are provided which are configured to implement side-scan infrared imaging for, e.g., medical applications. For example, an imaging device includes a ring-shaped detector element comprising a circular array of infrared detectors configured to detect thermal infrared radiation, and a focusing element configured to focus incident infrared radiation towards the circular array of infrared detectors. The imaging device can be an ingestible imaging device (e.g., swallowable camera) or the imaging device can be implemented as part of an endoscope device, for example.

A measurement apparatus comprises optical components arranged to provide parallel measurements of a biological sample. The parallel sample measurements provide improved accuracy with lower detection limit thresholds. The parallel measurements may comprise one or more of Raman spectroscopy measurements or infrared spectroscopy measurements. The parallel measurements can be combined with a light source. In many embodiments, the light source comprises one or more wavelengths corresponding to resonance frequencies of one or more molecules of the sample, such as wavelengths of ultraviolet light. The wavelengths of light corresponding to resonance frequencies can provide an increased signal to noise ratio. The parallel array optical configuration can be combined with wavelengths of light corresponding to resonance frequencies in order to provide increased measurement accuracy and detection of metabolites.

Devices, systems, and methods are disclosed for improved laser speckle imaging of samples, such as vascularized tissue, for the determination of the rate of movement of light scattering particles within the sample. The system includes a structure adjoining a light source and a photo-sensitive detector. The structure can be positioned adjacent the sample (e.g., coupled to the sample) and configured to orient the light source and detector relative the sample such that surface reflections, including specular reflections and diffuse reflections, are discouraged from entering the detection field of the detector. The separation distance along the structure between the light source and the detector may further enable selective depth penetration into the sample and biased sampling of multiply scattered photons. The system includes an operably coupled processor programmed to derive contrast metrics from the detector and to relate the contrast metrics to a rate of movement of the light scattering particles.

A wavefront control apparatus includes a detector configured to detect a signal generated from a medium onto which light is irradiated, and a controller configured to control a wavefront of the light based on an output of the detector. The controller performs first processing for forming a first wavefront of the light based on the signal generated from a first measurement position in the medium, and second processing for forming a second wavefront of the light based on the signal generated from a second measurement position different from the first measurement position in the medium onto which the light having the first wavefront is irradiated.

A method of analysis using mass spectrometry and/or ion mobility spectrometry is disclosed comprising: (a) using a first device to generate smoke, aerosol or vapour from a target in vitro or ex vivo cell population; (b) mass analysing and/or ion mobility analysing said smoke, aerosol or vapour, or ions derived therefrom, in order to obtain spectrometric data; and (c) analysing said spectrometric data in order to identify and/or characterise said target cell population or one or more cells and/or compounds present in said target cell population.

Embodiments of the invention provide a method and system that allows parameters of a desired target image to be determined from hyperspectral imagery of scene. The parameters may be representative of various aspects of the scene being imaged, particularly representative of physical properties of the scene. For example, in some medical imaging contexts, the property being imaged may be blood perfusion or oxygenation saturation level information per pixel. In one embodiment the parameters are obtained by collecting lower temporal and spatial resolution hyperspectral imagery, and then building a virtual hypercube of the information having a higher spatial resolution using a spatiospectral aware demosaicking process, the virtual hypercube then being used for estimation of the desired parameters at the higher spatial resolution. Alternatively, in another embodiment, instead of building the virtual hypercube and then performing the estimation, a joint demosaicking and parameter estimation operation is performed to obtain the parameters. Various white level and spectral calibration operations may also be performed to improve the results obtained. While establishing functional and technical requirements of an intraoperative system for surgery, we present iHSI system embodiments that allows for real-time wide-field HSI and responsive surgical guidance in a highly constrained operating theatre. Two exemplar embodiments exploiting state-of-the-art industrial HSI cameras, respectively using linescan and snapshot imaging technology, were investigated by performing assessments against established design criteria and ex vivo tissue experiments. We further report the use of one real-time iHSI embodiment during an ethically-approved in-patient clinical feasibility case study as part of a spinal fusion surgery therefore successfully validating our assumptions that our invention can be seamlessly integrated into the operating theatre without interrupting the surgical workflow.