Described are compositions and methods relating to phosphotransacetylase (PTA) genes that improve ethanol production in yeast harboring an engineered PKL pathway, and yeast expressing these PTA genes. Such yeast is particularly useful for large-scale ethanol production from starch substrates, where acetate in an undesirable by-product.

The present invention relates to a leader peptide which promotes the secretion of recombinant proteins and a nucleic acid sequence encoding the leader peptide as well as expression cassettes, vectors and host cells comprising this leader sequence. Also disclosed is a method for producing a protein using this leader peptide.

Provided is a hepatitis B treatment vaccine based on an inactivated whole recombinant Hansenula polymorpha cell which expresses HBsAg and HBcAg. An HBsAgVLP and an HBcAgVLP expressed in the recombinant Hansenula polymorpha cell are used as antigens, the amino acid sequence of the HBsAg expressed by the recombinant Hansenula polymorpha contains a total of 19 CTL epitopes, the amino acid sequence of the HBcAg expressed by the recombinant Hansenula polymorpha contains a total of 19 CTL epitopes, and the inactivated whole recombinant Hansenula polymorpha cell is used as an adjuvant.

According to exemplary embodiments, provided herein are methods for nutrient fortification, including determining a nutrient profile for a first food product, modeling a micronutrient profile based on the nutrient profile, culturing yeast in the micronutrient profile and applying the cultured yeast to a second food product.

A DNA sequence encoding Der p1 protein having a particular base sequence is codon-optimized for the Pichia pastoris expression system, which is conducive to expressing Der p1 in Pichia pastoris. After gene optimization and adding an activating element to increase the expression of Der p1 in molecular level, it was found that Der p1 is expressed at a higher level as compared with the prior art and has biological activity similar to the natural protein.

Disclosed is oleaginous yeast and its application, belonging to the technical field of microorganisms. The oleaginous yeast provided by the disclosure has been deposited in China Center for Type Culture Collection on May 21, 2020, with an accession number of CCTCC NO: M 2020139. The oleaginous yeast provided by the disclosure can use a hydrolysate of cheap lignocellulose biomass as the substrate. Xylose and glucose in the hydrolysate are simultaneously utilized to produce oil, so the sugar utilization efficiency and production intensity are enhanced. After 8 days of fed-batch fermentation, the oil yield can reach 31.33 g/L, and the oil content can reach 60.83%. The waste resources can be reutilized, and the production cost is reduced, so the oleaginous yeast has wide application prospects.

The present invention relates to a method for producing fermented and vacuum-treated coffee using Kopi Luwak enterobacteria, in which the coffee contains a low concentration of caffeine and a high concentration of γ-aminobutyric acid (GABA). According to the present invention, the fermented and vacuum-treated coffee produced using Kopi Luwak enterobacteria according to the present invention has advantages in that it is produced by rapid fermentation, uniquely smells like caramel, chocolate, grass, etc., unlike unfermented green coffee beans, and has a reduced bitter taste and a deep and heavy taste with an appropriate sour taste.

Provided is a Pichia pastoris mutant strain for expressing an exogenous gene. Specifically, provided is a Pichia pastoris mutant strain comprising, with respect to Pichia pastoris mutant strain GS115 or CICC32806, one or more of the following six mutations: BQ9382_C1-2260, EKK deletions at positions 308-310, a hypothetical protein; BQ9382_C1-3800, E129K, 60S ribosomal subunit assembly/exported protein LOC1; BQ9382_C1-5700, I312M, mitochondrial external NADH dehydrogenase, type II NAD(P)H:quinone oxidoreductase; BQ9382_C2-3950, Q145X, an essential protein having a binding partner Psr1p and used for completely activating a general stress response; BQ9382_C3-2220, E188K, a hypothetical protein; and BQ9382_C3-4370, W196X, orotidine 5\′-phosphate decarboxylase. The provided Pichia pastoris mutant strain is an effective commonly employed host for exogenous expression, and can efficiently express different proteins, especially phospholipase and lipase.

A microorganism of the present invention is Dirkmeia churashimaensis (NITE BP-03054), Papiliotrema flavescens (NITE BP-03051), Papiliotrema flavescens (NITE BP-03052), or Apiotrichum porosum (NITE BP-03053).

A process for the preparation of bio-based malonic acid and crystalline calcium malonate is provided. The calcium malonate is highly pure and provides a source of malonic acid made from a renewable carbon source rather than existing processes which rely on the use of petroleum-based products. The calcium malonate provides an improved source of malonic acid, which is important to many industrial processes.