Disclosed herein are methods for production of 2-phenylethanol by microbial fermentation of substrates comprising carbon monoxide and/or carbon dioxide and further disclosed are genetically modified microorganisms for use in such methods. Additionally, the processes disclosed herein are improved methods of 2-PE production that alleviate dependence on natural and petrochemical processes.

The present invention belongs to the field of plant genetic engineering. Specifically, the invention relates to a method for creating novel herbicide resistant plants by base editing techniques and a method for screening endogenous gene mutation sites capable of conferring herbicide resistance in plants. The invention also relates to the use of the identified endogenous gene mutantation sites in crop breeding.

The invention relates to novel methods and compositions for conferring tolerance to glyphosate to plants. The invention also provides glyphosate-tolerant plants, seeds, tissue, cells, and plant parts comprising modified EPSP synthases and recombinant DNA molecules encoding modified EPSP synthases, as well as methods of producing the same and the use thereof.

The invention relates generally to peptide biomarkers with specific ionization characteristics to directly quantify one or more transgenic target proteins in biological samples, including transgenic plant samples, by liquid chromatography coupled tandem mass spectrometry multiple reaction monitoring (MRM). The peptide biomarkers in combination with MRM-based methods may be used to quantify a single transgenic target protein or multiple transgenic target proteins within a stacked transgenic crop, such as maize, utilizing selected peptide biomarkers either alone or in combination. The present disclosure allows for broad based, reliable quantitation in different biological matrices, including plant matrices. The peptide biomarkers of the invention can further be used as trait biomarkers to support identification and/or selection of specific transgenic Events. Also provided are different peptide biomarker combinations that can be used to perform the methods of the invention.

This invention relates in part to soybean event pDAB8264.44.06.1 and includes a novel expression cassettes and transgenic inserts comprising multiple traits conferring resistance to glyphosate, aryloxyalkanoate, and glufosinate herbicides. This invention also relates in part to methods of controlling resistant weeds, plant breeding and herbicide tolerant plants. In some embodiments, the event sequence can be “stacked” with other traits, including, for example, other herbicide tolerance gene(s) and/or insect-inhibitory proteins. This invention further relates in part to endpoint TaqMan PCR assays for the detection of Event pDAB8264.44.06.1 in soybeans and related plant material. Some embodiments can perform high throughput zygosity analysis of plant material and other embodiments can be used to uniquely identify the zygosity of and breed soybean lines comprising the event of the subject invention. Kits and conditions useful in conducting these assays are also provided.

A paddy rice EPSPS mutant, and an encoding gene and use thereof, relating to the technical field of genetic engineering. The paddy rice EPSPS mutant has an amino acid sequence shown as SEQ ID NO. 1. The paddy rice EPSPS mutant has glyphosate resistance and can resist glyphosate having a concentration of 100 mM. The paddy rice EPSPS mutant has very wide use prospect in the field of cultivating glyphosate-resistant plants.

Provided are a glyphosate-resistant gene screening method, an EPSPS mutant gene having glyphosate resistance screened by the method, an EPSPS and C-P Lyase deficient strain and a use thereof.

Provided are a plant EPSPS mutant containing mutations L195P and S247G and an encoding gene and the use thereof, related to the field of genetic engineering technology. Comparing the plant EPSPS mutant with E. coli EPSPS, the amino acid sequence of the plant EPSPS mutant has the mutation L195P at position 195 corresponding to E. coli EPSPS and/or the mutation S247G at position 247 corresponding to E. coli EPSPS. The mutation of either of the two sites or the simultaneous mutation of the two sites can confer or improve the resistance of the plant EPSPS mutant to glyphosate. Plants or recombinant bacteria for transforming the plant EPSPS mutant can grow normally in the presence of glyphosate.

Plants with resistance to glyphosate are disclosed herein. In one embodiment, the disclosure relate to human induced non-transgenic mutations in the EPSPS gene in plants.

Bacterial cultures are provided that comprise a modified Pseudomonas aeruginosa bacterium missing or deficient in two or more virulence factors. The two or more virulence factors can be selected from exotoxin A, hemolytic phospholipase C, phenazine-specific methyltransferase, alpha-1,3-rhamnosyltransferase, and 3-phosphoshikimate 1-carboxyvinyltransferase. Certain of the modified Pseudomonas aeruginosa bacteria are also missing or deficient in one or more alginate acetylation enzymes including the alginate Oacetyltransferases AlgI, AlgJ, AlgF, AlgX, and/or the C5-mannuronan epimerase AlgG. Methods of producing alginate are also provided along with compositions comprising alginate produced by the modified Pseudomonas aeruginosa bacteria.