Disclosed are a novel Korean isolate of tomato yellow leaf curl virus (TYLCV) and infectious clones thereof, wherein: the use of an inventive recombinant vector comprising the nucleotide sequence of tomato yellow leaf curl virus (TYLCV) and Agrobacterium tumefaciens comprising the recombinant vector can effectively induce tomato yellow leaf curl virus infection in a crop even without an insect vector; an inventive infection system, compared with existing TYLCV Korean isolates, shows excellent infectivity in a plant, and thus can induce symptoms even in TYLCV-resistant varieties of tomatoes having existing widely used Ty-1 or Ty-2 resistant gene, so that the infectious system can be utilized as an artificial infection system in the future and can be applied in various studies in the future, such as a host plant range for tomato yellow leaf curl virus (TYLCV) and the correlation with the host plant.

An object of the present invention is to provide a novel method of controlling TYLCV whereby the infection cycle of TYLCV can be broken. The present invention provides a novel virus that is not transmitted by an insect vector as a tomato yellow leaf curl virus. The present invention also provides a method of controlling a tomato yellow leaf curl disease and a method of preventing transmission of a yellow leaf curl virus by using a tomato yellow leaf curl virus not transmitted by an insect vector.

A method for simply constructing a two-component virus vector and related applications. The two-component plant viral vector is optimized, so that a two-component virus genome is placed in a single plasmid. Due to the use of the single plasmid, the activation, resuspension and mixing processes of auxiliary bacteria are avoided, the workload of an Agrobacterium experimental stage is simplified by half, the potential cross-contamination risk in the bacteria mixing process is avoided, and the virus infectivity is improved, so that the use of the two-component viral vector is greatly simplified.

A method for simply constructing a two-component virus vector and related applications. The two-component plant viral vector is optimized, so that a two-component virus genome is placed in a single plasmid. Due to the use of the single plasmid, the activation, resuspension and mixing processes of auxiliary bacteria are avoided, the workload of an Agrobacterium experimental stage is simplified by half, the potential cross-contamination risk in the bacteria mixing process is avoided, and the virus infectivity is improved, so that the use of the two-component viral vector is greatly simplified.