Methods of culturing and detecting a biomarker which may be associated with autoimmune diseases, in particular inflammatory bowel disease and Crohn's disease; also a screening method for substances suitable for the treatment of inflammatory bowel disease including Crohn's disease; the method including culturing a biomarker in a culture media which includes a culture broth, OADC, PANTA and Mycobactin J.

A method for evaluating a condition of cells including: (A) measuring first exosomes having a first exosome membrane protein and second exosomes having a second exosome membrane protein in a culture supernatant of the cells; (B) calculating the ratio of the second exosomes to the first exosomes; and (C) determining the condition of the cells using the ratio as an index.

A method for evaluating a condition of cells including: (A) measuring first exosomes having a first exosome membrane protein and second exosomes having a second exosome membrane protein in a culture supernatant of the cells; (B) calculating the ratio of the second exosomes to the first exosomes; and (C) determining the condition of the cells using the ratio as an index.

The invention is directed towards methods and compositions for identifying the specific microorganisms present in a particular potion of a water process system. The method involves obtaining a sample from the process and determining if a problematic organism exceeds an acceptable threshold. If so a remedial bio-control procedure can be applied long before any unwanted defects or problems occur.

The invention is directed towards methods and compositions for identifying the specific microorganisms present in a particular potion of a water process system. The method involves obtaining a sample from the process and determining if a problematic organism exceeds an acceptable threshold. If so a remedial bio-control procedure can be applied long before any unwanted defects or problems occur.

The invention enables efficient, rapid, and sensitive enumeration of living cells by detecting microscopic colonies derived from in situ cell division using large area imaging. Microbial enumeration tests based on the invention address an important problem in clinical and industrial microbiology—the long time needed for detection in traditional tests—while retaining key advantages of the traditional methods based on microbial culture. Embodiments of the invention include non-destructive aseptic methods for detecting cellular microcolonies without labeling reagents. These methods allow for the generation of pure cultures which can be used for microbial identification and determination of antimicrobial resistance.

The invention enables efficient, rapid, and sensitive enumeration of living cells by detecting microscopic colonies derived from in situ cell division using large area imaging. Microbial enumeration tests based on the invention address an important problem in clinical and industrial microbiology—the long time needed for detection in traditional tests—while retaining key advantages of the traditional methods based on microbial culture. Embodiments of the invention include non-destructive aseptic methods for detecting cellular microcolonies without labeling reagents. These methods allow for the generation of pure cultures which can be used for microbial identification and determination of antimicrobial resistance.

The present disclosure relates to methods of diagnosing a dysbiosis in a subject, methods of determining a suitable treatment, and methods of treating a dysbiosis. In some aspects, the present disclosure relates to diagnosing or determining a subtype of irritable bowel syndrome (IBS).

The present disclosure relates to methods of diagnosing a dysbiosis in a subject, methods of determining a suitable treatment, and methods of treating a dysbiosis. In some aspects, the present disclosure relates to diagnosing or determining a subtype of irritable bowel syndrome (IBS).

The invention generally relates to analyzing yeast viability. More particularly, the invention relates to efficient and effective methods and compositions for accessing and measuring viability and concentration of yeast cells.