The invention generally relates to analyzing yeast viability. More particularly, the invention relates to efficient and effective methods and compositions for accessing and measuring viability and concentration of yeast cells.

A rapid assay for determining the presence of bacteria in a sample, such as a contaminated food sample, is disclosed. The assay comprises contacting the sample with a bacteria-specific ligand associated with a substrate, wherein bacteria present in the sample bind the ligand; contacting the bound bacteria with a detection agent; detecting the presence of bacteria in the sample by measuring the quantity of detection agent associated with the sample.

A rapid assay for determining the presence of bacteria in a sample, such as a contaminated food sample, is disclosed. The assay comprises contacting the sample with a bacteria-specific ligand associated with a substrate, wherein bacteria present in the sample bind the ligand; contacting the bound bacteria with a detection agent; detecting the presence of bacteria in the sample by measuring the quantity of detection agent associated with the sample.

The present disclosure relates to approaches for assessing a sample or the presence of microorganisms. The sample, in certain implementations may be assessed for one or both of absence of microorganisms (sterility) and/or for concentration of said organisms (bio-burden). sample partition device may be employed that partitions the sample input volume into multiple discrete measurement zones with little or no loss of sample (e.g., zero-loss) and with little operator involvement, thereby reducing operator- and environment-based false positives.

The present disclosure relates to approaches for assessing a sample or the presence of microorganisms. The sample, in certain implementations may be assessed for one or both of absence of microorganisms (sterility) and/or for concentration of said organisms (bio-burden). sample partition device may be employed that partitions the sample input volume into multiple discrete measurement zones with little or no loss of sample (e.g., zero-loss) and with little operator involvement, thereby reducing operator- and environment-based false positives.

A method of selecting a ruminating animal having a desirable, hereditable trait is disclosed. The method comprises analyzing in the microbiome of the animal for an amount of a hereditable microorganism which is associated with the hereditable trait, wherein the amount of the hereditable microorganism is indicative as to whether the animal has a desirable hereditable trait.

One variation of a method for detecting pathogens in an environment includes, during a first sampling period: triggering collection of a pathogen sample from ambient air in the environment by an air sampler; and tracking a first organic load of the first pathogen sample via a detection subsystem integrated within the air sampler, the first organic load representative of a first amount of organic matter present in the first pathogen sample. In response to the first organic load exceeding a threshold organic load defined for the environment, the method further includes: interpreting presence of a set of pathogens in the environment via genetic analysis of the first pathogen sample; and, in response to detecting presence of a first pathogen, in the set of pathogens, in the first pathogen sample, transmitting a notification indicating presence of the first pathogen in the environment to a user associated with the environment.

One variation of a method for detecting pathogens in an environment includes, during a first sampling period: triggering collection of a pathogen sample from ambient air in the environment by an air sampler; and tracking a first organic load of the first pathogen sample via a detection subsystem integrated within the air sampler, the first organic load representative of a first amount of organic matter present in the first pathogen sample. In response to the first organic load exceeding a threshold organic load defined for the environment, the method further includes: interpreting presence of a set of pathogens in the environment via genetic analysis of the first pathogen sample; and, in response to detecting presence of a first pathogen, in the set of pathogens, in the first pathogen sample, transmitting a notification indicating presence of the first pathogen in the environment to a user associated with the environment.

Disclosed are methods of isolating T cells and TCRs having antigenic specificity for a mutated amino acid sequence encoded by a cancer-specific mutation. Also disclosed are related methods of preparing a population of cells, populations of cells, TCRs, pharmaceutical compositions, and methods of treating or preventing cancer.

The disclosure provides methods and compositions for the preservation of bacteria.