A CRISPR electrochemical biosensing system (E-CRISPR) for detection of analytes includes a disposable, micro-fabricated three-electrode sensor that includes a working electrode, a counter electrode, a reference electrode, and a nonspecific ssDNA reporter with an electrochemical tag for signal transduction tethered to a surface of the working electrode; and a Cas12a-crRNA duplex that is designed to specifically recognize and cleave target nucleic acid strand based on the protospacer adjacent motif (PAM) sequence of the target and crRNA sequence, wherein the PAM recognition depends on specific 5′ TTTN nucleic acid sequence located at an opposite strand of a recognition strand, and wherein only upon the recognition of the PAM sequence by the Cas protein, the Cas protein, acting as a DNA helicase, unwinds the target DNA.

There is provided a method of detecting the presence of a nucleic acid target sequence in which two oligonucleotides are used to forma three-way junction with the target sequence to allow detection of the target sequence. Alternatively, three oligonucleotides can be used to form a four-way junction with the target sequence to allow detection of the target sequence.

There is provided a method of detecting the presence of a nucleic acid target sequence in which two oligonucleotides are used to forma three-way junction with the target sequence to allow detection of the target sequence. Alternatively, three oligonucleotides can be used to form a four-way junction with the target sequence to allow detection of the target sequence.

Methods of analyzing nucleic acids of a cell are provided.

Methods of analyzing nucleic acids of a cell are provided.

Disclosed herein is a transmembrane nanosensor device comprising a lipid conjugated DNA tweezer, and methods of using the same.

Disclosed herein is a transmembrane nanosensor device comprising a lipid conjugated DNA tweezer, and methods of using the same.

The present invention describes a method of prognosing high or low probability of developing an inflammatory bowel disease (IBD) in a subject and a method of diagnosing an inflammatory bowel disease (IBD) in a subject. The invention further provides for a method of identifying genes/genetic loci associated with a disease condition, such as IBD, CD and/or UC.

The present invention describes a method of prognosing high or low probability of developing an inflammatory bowel disease (IBD) in a subject and a method of diagnosing an inflammatory bowel disease (IBD) in a subject. The invention further provides for a method of identifying genes/genetic loci associated with a disease condition, such as IBD, CD and/or UC.

A method for analyzing target nucleic acid from a cell, including: 1) providing a discrete partition: target nucleic acid derived from a single cell and added with an oligonucleotide adaptor sequence, and a solid support with at least one oligonucleotide tag attached, wherein each oligonucleotide tag includes a first and second strand, the first strand includes a barcode sequence and a hybridization sequence located at the 3′-end of the barcode sequence, the second strand includes a first portion, complementary to the hybridization sequence of the first strand, and a second portion, complementary to the oligonucleotide adaptor sequence attached to the target nucleic acid, and the first and second strand form a partial double-strand, or the second strand and target nucleic acid attached form a partial double-strand; and (2) in the discrete partition, the oligonucleotide tag is linked to the target nucleic acid attached, thereby producing barcoded target nucleic acid.