The present invention relates to a method for differentiating hair follicle cells into germline stem cells, germline stem cells differentiated by the method, and use of the same germline stem cells. A method of differentiating hair follicle cells into germline stem cells according to the present invention can differentiate hair follicle cells into germline stem cells using culture conditions only, without genetic modification. Capable of inducing differentiation of cells of specific individual types, such as hair follicle cells, into cells of different types such as germline cells, the present invention is therefore expected to be usefully used for the understanding of reproductive biology and the clinical application thereof.

The present invention relates to compositions of minoxidil and Sonic Hedgehog (Shh) pathway activators and optionally, Wnt agonists and methods of using them to induce self-renewal of hair follicle stem cells, including inducing the hair follicle stem cells to proliferate while maintaining, in the daughter cells, the capacity to differentiate into hair follicle epithelial cells.

The present invention relates to compositions of Sonic Hedgehog (Shh) pathway activators and Wnt agonists and methods of using them to induce self-renewal of hair follicle stem cells, including inducing the hair follicle stem cells to proliferate while maintaining, in the daughter cells, the capacity to differentiate into hair follicle epithelial cells.

The present disclosure provides a reprosome that can induce reprogramming of a cell, in which the reprosome is characterized by including RNA of a gene involved in chromatin remodeling, in which the gene includes a kinase gene on a mitogen-activated protein kinase (MAPK) signal transduction system, and a gene having histone modification activity. The reprosome may be obtained from a stem cell difficult to process and/or from a readily obtainable somatic cell via a simple process including ultrasonic treatment. A reprogramming of one kind of a cell into another kind of a cell with a desired function can be achieved at a high efficiency in a short time via a simple treatment including a co-culturing between the reprosome and the cell. The cell thus obtained has the desired function without introduction of a chemical or foreign transcription factor into a genome and thus is more suitable for cell replacement therapies. Further, the present disclosure provides a composition including the reprosome and a method for regenerating a tissue by treating a body site with the composition to promote reprogramming of a cell present in the treated body site to a target cell having a desired function.

The present invention relates to compositions and methods utilizing hair follicle derived Non-Bulbar Dermal Sheath cells for use in the treatment or prevention of the tendon injuries.

A composition and in vitro method for generating a desired cell type and/or tissue type from hair follicular stem cells. The composition and in vitro method are particularly suitable for generating an autologous desired cell type and/or tissue type. Furthermore, the composition and method are especially efficient and suitable for use in the context of cosmetic cell and/or tissue transplantation in recipient areas of a subject experiencing cell and/or tissue loss caused by, for example, a wound, scar, burn injury, tissue degeneration, and aging. The composition and in vitro method are also suitable to circumvent complications related to infections and/or immune rejection of a cosmetic cell and/or tissue implant or graft.

Cosmetic methods providing a significant improvement over existing hair transplantation methods by providing greater hair density per hair implant, which is achieved through the process of an enhanced hair multiplication in vivo (e.g. in the scalp of a subject). The cosmetic methods also provide improved aesthetic results, particularly, more realistic and natural looking results, relative to the results obtained by traditional methods. The cosmetic methods are particularly suitable for hair transplantation in recipient areas of a subject experiencing baldness or lack of hair, optionally due to Androgenic alopecia, burn injuries, cancer chemotherapy, or other genetic or environmental factors or scarring.

The disclosure provides a cell culturing article. The cell culturing article includes a substrate having a surface, a hydrophilic copolymer layer positioned on the surface of the substrate, and a plurality of peptide chains individually conjugated to a surface of the hydrophilic copolymer layer. The hydrophilic copolymer layer is copolymerized by a plurality of polyvinyl alcohol units, a plurality of polyvinyl alcohol derivative units, and a plurality of units containing at least one carboxyl group. A method for manufacturing the cell culturing article, a method for culturing undifferentiated stem cells and a method for regulating stem cell differentiation are also provided herein.

The present invention is concerned with a composition and in vitro method for generating a desired cell type and/or tissue type from hair follicular stem cells. The composition and in vitro method are particularly suitable for generating an autologous desired cell type and/or tissue type. Furthermore, the composition and method are especially efficient and suitable for use in the context of cosmetic cell and/or tissue transplantation in recipient areas of a subject experiencing cell and/or tissue loss caused by, for example, a wound, scar, burn injury, tissue degeneration, and aging. The composition and in vitro method are also suitable to circumvent complications related to infections and/or immune rejection of a cosmetic cell and/or tissue implant or graft.

The present invention relates to compositions and methods utilizing hair follicle derived Non-Bulbar Dermal Sheath cells for use in the treatment or prevention of the tendon injuries.