C12N15/8212

Methods to Monitor Post-Translational Gene Silencing Activity in Plant Tissues/Cell Types Relevant for Pathogen Entry, Propagation or Replication
20170349907 · 2017-12-07 ·

The invention relates to transgenic plants comprising an inverted-repeat construct which triggers post-transcriptional gene silencing of an endogenous visual reporter gene driven by a tissue-specific promoter wherein said tissue is relevant for pathogen entry, propagation or replication and their uses for screening natural or synthetic molecules, microorganisms or extracts from micro- or macro-organisms for their potential ability to inhibit pathogen entry, propagation or replication in plants by enhancing PTGS or for characterizing the mode of action of natural or synthetic molecules that are known to enhance plant disease resistance through an ill-defined mode of action.

TOMATO PLANTS HAVING SUPPRESSED MEIOTIC RECOMBINATION
20230183737 · 2023-06-15 ·

The present invention relates to a tomato plant comprising in its genome at least one chromosome comprising a mutant allele of the wild type male sterility 10 (MS10) gene and a mutant allele of the wild type anthocyanin absent (AA) gene wherein in said plant the meiotic recombination frequency is reduced between said mutant allele of the wild type MS10 gene and said mutant allele of the wild type AA gene when compared to the meiotic recombination frequency between the MS10 gene and the AA gene in a wild type Solanum lycopersicum plant. The present invention further relates to a seed from which a plant according to present invention can be grown and a part of a plant according to the present invention. The present invention further relates to a method of identifying and/or selecting a male sterile plant, said method comprising growing a plant according to the present invention and determining whether anthocyanin is absent in the hypocotyls of said plant. The present invention further relates to a method of identifying and/or selecting a plant or plant part according to the present invention. The present invention further relates to a method of producing tomato plant or tomato plant part having male sterility and anthocyanin absent hypocotyls, wherein in said plant or plant part the meiotic recombination frequency between the male sterility trait and the anthocyanin absent hypocotyls trait is reduced when compared to the meiotic recombination frequency between the MS10 gene and the AA gene in a wild type Solanum lycopersicum plant.

DOMINANT GENE SUPPRESSION TRANSGENES AND METHODS OF USING SAME

Pairs of plants are provided in which complementing constructs result in suppression of a parental phenotype in the progeny. Methods to generate and maintain such plants and methods of use of said plants, are provided, including use of parental plants to produce sterile plants for hybrid seed production. Also provided are methods for maintaining a homozygous recessive condition and for repressing transmission of transgenes.

Light-absorbing compositions and methods of use

The present disclosure relates to methods and compositions comprising naturally occurring light absorbing molecules for preventing damages from light exposure. Specific embodiments of this disclosure include fluorescent proteins from Brachiostoma lanceolatum.

Nanobionic engineering of organelles and photosynthetic organisms

In one aspect, a composition can include an organelle, and a nanoparticle having a zeta potential of less than −10 mV or greater than 10 mV contained within the organelle. In a preferred embodiment, the organelle can be a chloroplast and the nanoparticle can be a single-walled carbon nanotube associated with a strongly anionic or strongly cationic polymer.

Photoprotein, Substrate Thereof and Use of Same
20210371477 · 2021-12-02 · ·

Provided is a method for causing a subject including a luminescent protein to emit light. The method provided is a method for causing a subject to emit light, wherein the subject is a plant body that has a fusion luminescent protein in a cell wall thereof, or a processed product of the same; the method includes the step of bringing a substrate composition into contact with the fusion luminescent protein; the fusion luminescent protein is a protein that includes a chemiluminescent protein moiety, a fluorescent protein moiety, and a moiety that connects the chemiluminescent protein moiety and the fluorescent protein moiety so that resonance energy transfer can occur from the chemiluminescent protein moiety to the fluorescent protein moiety; and the substrate composition contains a substrate of the chemiluminescent protein.

COLOR-BASED AND/OR VISUAL METHODS FOR IDENTIFYING THE PRESENCE OF A TRANSGENE AND COMPOSITIONS AND CONSTRUCTS RELATING TO THE SAME
20230266293 · 2023-08-24 ·

Described herein are color-based and/or visual methods for identifying the presence of a transgene (e.g., the presence of a transgene in a cell, seed, plant part, and/or plant) along with composition, systems, and constructs relating to the same.

Methods for producing transgenic plants

A method of producing a stably transformed corn plant in a single container is demonstrated. This method allows for the automation of the transformation process and reduces labor, material, and ergonomic costs associated with traditional plant tissue culture systems.

METHODS FOR PRODUCING TRANSGENIC PLANTS

A method of producing a stably transformed corn plant in a single container is demonstrated. This method allows for the automation of the transformation process and reduces labor, material, and ergonomic costs associated with traditional plant tissue culture systems.

SPRAYABLE CELL-PENETRATING PEPTIDES FOR SUBSTANCE DELIVERY IN PLANTS

The invention relates to a complex comprising a Cell Penetrating Peptide and one or more nucleic acids which can be applied to a plant by spraying and which can trigger a physiological outcome. Hereto, the one or more nucleic acids complex with a Cell Penetrating Peptide can be dissolved in water without the presence of additional components in the solution.