The present disclosure provides recombinant nucleic acids comprising one or more polynucleotides encoding one or more cosmetic proteins (e.g., one or more human collagen proteins); viruses comprising the recombinant nucleic acids; compositions (e.g., cosmetic formulations) comprising the recombinant nucleic acids and/or viruses; methods of their use; and articles of manufacture or kits thereof.

A mesenchymal stem cell extract and its use are provided, wherein the mesenchymal stem cell extract comprises a trophic factor(s), such as bone morphogenetic protein-7 (BMP-7), stromal cell-derived factor-1 (SDF-1), vascular endothelial growth factor (VEGF), C-X-C chemokine receptor type-4 (CXCR4), brain-derived neurotrophic factor (BDNF), and/or interleukin-17 (IL-17), and wherein the extract is especially suitable for repairing skin aging.

A therapeutic serum suitable for inclusion in a cosmetic preparation may be produced by stressing a co-culture including proliferative cells. The co-culture of cells may be obtained by first establishing a monolayer of cells on a surface. After a monolayer of first culture is established, a second culture comprising more resilient and/or aggressive cells may be over-seeded on the monolayer and established. Additional cultures comprising increasingly dominant cells may then be over-seeded and established until a monolayer having the desired population of cells is obtained. The monolayer is then stressed to obtain a serum by conditioning a collection medium. The obtained serum may be combined with a suitable cosmetic base to provide a cosmetic preparation.

A method for inducing tailored pluripotent stem cells by reprogramming differentiated cells of an adult by using an extract of plant stem cells and plant dedifferentiated stem cells (callus); pluripotent stem cells produced by means of the method; and a cell therapy agent comprising the pluripotent stem cells are disclosed. It is possible to overcome ethical problems since eggs are not used in the making of pluripotent stem cells having abilities like embryonic stem cells. It is possible to produce pluripotent stem cells of ensured safety because a plant stem cell extract which above all has been verified to be harmless to the body is used. It is possible to develop an immunocompatible cell therapy agent tailored to different individuals.

A therapeutic serum suitable for inclusion in a cosmetic preparation may be produced by stressing a co-culture including proliferative cells. The co-culture of cells may be obtained by first establishing a monolayer of cells on a surface. After a monolayer of first culture is established, a second culture comprising more resilient and/or aggressive cells may be over-seeded on the monolayer and established. Additional cultures comprising increasingly dominant cells may then be over-seeded and established until a monolayer having the desired population of cells is obtained. The monolayer is then stressed to obtain a serum by conditioning a collection medium. The obtained serum may be combined with a suitable cosmetic base to provide a cosmetic preparation.

The invention provides compositions comprising at least one rhamnolipid and at least one siloxane, characterized in that the weight ratio of rhamnolipid to siloxane is from 5,000,000:1 to 100:1, preferably from 500,000:1 to 1000:1, particularly preferably from 25,000:1 to 2500:1. In another embodiment, the invention provides compositions include rhamnolipid in an amount of from 0.5% by weight to 70% by weight, preferably from 2% by weight to 60% by weight, particularly preferably from 3% by weight to 50% by weight, where the percentages by weight refer to the total composition.

Disclosed herein is a method of preparing a subject's cells for topical treatment of a subject in need. Also provided is a topical formulation and methods of treating a subject suffering a skin disorder and pain using the topical formulation. Also disclosed herein are devices and systems for micronizing an aspirate in preparation of such a topical formulation.

A cosmetic composition for skin whitening, wrinkle improvement or skin regeneration includes, as an active ingredient, exosomes derived from stem cells comprising proliferating stem cells.

Disclosed herein is a method of preparing a subject's cells for topical treatment of a subject in need. Also provided is a topical formulation and methods of treating a subject suffering a skin disorder and pain using the topical formulation. Also disclosed herein are devices and systems for micronizing an aspirate in preparation of such a topical formulation.

A composition for inducing differentiation into adipocytes or regenerating adipose tissues comprises, as an ingredient, exosomes derived from stem cells differentiating into adipocytes, or exosomes derived from proliferating stem cells.