The present invention discloses a sterile PRP separation kit that has compartmentalized container having a cover that allows for a stage-specific exposure of sterile components of the sterile PRP separation kit housed within stage-specific compartments to a non-sterile environment commensurate with a specific stage of operation of a separation process of PRP. The sterile PRP separation kit includes a PRP tube with segregated portals for injection of blood into the PRP tube, aspiration of PRP from the PRP tube, and for maintaining an interior pressure of the PRP tube at equilibrium with ambient pressure during both injection and aspiration.

Embodiments of the technology described herein are based upon the discoveries that neutrophil extracellular traps (NETs) provide a stimulus for thrombus formation and that NETs are present in stored blood products. Accordingly, some embodiments relate to methods of treating and preventing toxicity of NETs and thrombosis caused by NETs. Additional embodiments are directed towards methods of treating stored blood products to prevent transfusion-related injuries.

A variety of fill options is provided for a cell processing system. Certain options relate to a filling system associated with the cell processing system, the filling system comprising one or more filling stations, each with at least one container that may receive product from a container associated with a cell processing system. Other options relate to a syringe assembly that receives a product from a container associated with a cell processing system.

The invention relates to a system, comprising: a) a sample processing unit, comprising an input port and an output port coupled to a rotating container having at least one sample chamber, the sample processing unit configured provide a first processing step to a sample or to rotate the container so as to apply a centrifugal force to a sample deposited in the chamber and separate at least a first component and a second component of the deposited sample; and b) a sample separation unit coupled to the output port of the sample processing unit, the cell separation unit comprising separation column holder (42), a pump (64) and a plurality of valves (1-11) configured to at least partially control fluid flow through a fluid circuitry and a separation column (40) positioned in the holder, the separation column configured to separate labeled and unlabeled components of sample flowed through the column.

The invention relates to a system, comprising: a) a sample processing unit, comprising an input port and an output port coupled to a rotating container having at least one sample chamber, the sample processing unit configured provide a first processing step to a sample or to rotate the container so as to apply a centrifugal force to a sample deposited in the chamber and separate at least a first component and a second component of the deposited sample; and b) a sample separation unit coupled to the output port of the sample processing unit, the cell separation unit comprising separation column holder (42), a pump (64) and a plurality of valves (1-11) configured to at least partially control fluid flow through a fluid circuitry and a separation column (40) positioned in the holder, the separation column configured to separate labeled and unlabeled components of sample flowed through the column.

A system and methodology for the preservation of red blood cells is described in which red blood cells are oxygen or oxygen and carbon dioxide depleted, treated and are stored in an anaerobic environment to optimize preparation for transfusion. More particularly, a system and method for extended storage of red blood cells from collection to transfusion that optimizes red blood cells prior to transfusion is described.

Bag system (1) for collecting blood from an animal, comprising a collection bag (2) fluidically connected, via a first tubing (4) associated with an inlet tubing of the collection bag (2), to a first sampling needle (3), a second sampling needle (4) being connected via a second tubing (6), said second sampling needle (5) having an outer diameter that is different from the first sampling needle (3).

A blood component collection system, which is one form of a biological component collection system, is equipped with a centrifugal separation device and a biological component collection device. The biological component collection device comprises a pressed soft portion pressed by a load detecting unit. The centrifugal separation device includes a collection and returning pump, and a control unit having an internal pressure computation unit which calculates a circuit internal pressure of the biological component collection device on the basis of a detection value of the load detecting unit. The internal pressure computation unit performs a zero reset process of setting a pressure value corresponding to the detection value of the load detecting unit, so as to become zero at each instance of a predetermined timing at which the collection and returning pump is stopped.

It is an object of the present invention a method to introduce compounds inside red blood cells comprising: —providing red blood cells from a subject; —providing one or more compounds to be encapsulated in said red blood cells; —providing a loading device comprising a microporous matrix; —feeding said loading device with a suspension comprising said red blood cells and said one or more compounds; —collecting the red blood cells exiting from said loading device, which are encapsulated red blood cells; characterized in that: —said red blood cells and said one or more compound are in suspension at a pH of between 6.8 and 7.8, preferably of between 7.35 and 7.45; —the pores in said microporous matrix have a minimum size of at least 3 times the size of a red blood cell, that is a minimum size of at least 20 μm; —the pores in said microporous matrix have an average size of between 30 and 500 μm, or of between 40 and 400 μm, or of between 50 and 350 μm, or of between 100 and 250 μm; —said porous matrix has a length L of at least 1 mm and a width W and a height H such that it comprises at least one unit cell, said unit cell being equal to the microporous matrix volume that, repeated by rototranslation through the vectors that generate the matrix, fills the whole matrix itself; wherein said loading device is fed with said suspension in such a way to obtain an average fluid speed of between 10.sup.−4 and 10 m/s. Further objects of the present invention are a microporous matrix, a loading device comprising the same, a fluidic circuit and a machine for implementing said method and red blood cells encapsulated with at least one compound according to said method.

The invention relates to a system, comprising: a) a sample processing unit, comprising an input port and an output port coupled to a rotating container having at least one sample chamber, the sample processing unit configured provide a first processing step to a sample or to rotate the container so as to apply a centrifugal force to a sample deposited in the chamber and separate at least a first component and a second component of the deposited sample; and b) a sample separation unit coupled to the output port of the sample processing unit, the cell separation unit comprising separation column holder (42), a pump (64) and a plurality of valves (1-11) configured to at least partially control fluid flow through a fluid circuitry and a separation column (40) positioned in the holder, the separation column configured to separate labeled and unlabeled components of sample flowed through the column.