The present invention relates to novel bis-iridium-based Ir(III) complexes, labels manufactured using these complexes and a method for producing such complexes.

Devices, systems, and methods for detecting molecules of interest within a collected sample are described herein. In certain embodiments, self-contained sample analysis systems are disclosed, which include a reusable reader component, a disposable cartridge component, and a disposable sample collection component. In some embodiments, the reader component communicates with a remote computing device for the digital transmission of test protocols and test results. In various disclosed embodiments, the systems, components, and methods are configured to identify the presence, absence, and/or quantity of particular nucleic acids, proteins, or other analytes of interest, for example, in order to test for the presence of one or more pathogens or contaminants in a sample.

Methods of producing light in liquid media are provided using nanoparticles capable of generating electroluminescence when stimulated by an electrical signal. The nanoparticles are provided as a label on a target species or on a specific binding partner of the target species to be detected in a test method. The nanoparticle-labeled species are drawn into operable proximity to electrodes which, when energized by a power source, excite the nanoparticles to produce electroluminescence. Methods of performing binding assays are described using the disclosed methods.

The disclosure concerns methods for the detection of an analyte in a sample by electrochemiluminescence using new reagent compositions. New reagent compositions, reagent kits for measuring electrochemiluminscence (ECL) and electrochemiluminescence detection methods using the new reagent compositions are disclosed. In particular, the disclosure relates to the use of novel combinations of compounds which can be used in said measurements to provide improved assay performance.

The present invention relates to the use of a control marker for implementing analysis methods on spots, in particular in the context of multiplex analyses. The present invention thus relates to solid supports containing said control marker, their preparation method and their use in analysis methods. The present invention makes it possible to verify the presence, location and/or integrity of the spots at the end of the analysis method, and thus to secure the obtained results while guaranteeing that the yielded result indeed results from a present, intact and localized spot.

An electrochemiluminescence immunoassay system and a flow-through cell unit (1) thereof. The flow-through cell unit (1) comprises a working electrode (12), a counter electrode (11) and a reference electrode (13), wherein the working electrode (12) and the counter electrode (11) are vertically provided, and there is a liquid flow path between the two electrodes. When an electrochemical reaction occurs, and when reactants are evenly distributed on the working electrode (12) for a test. After the flow-through cell is cleaned, the liquid flow path is unblocked, so that the cleaning effect is better. A porous structure is provided on a connection surface where the reference electrode (13) is in communication with the liquid flow path, and the porous structure makes the reference electrode (13) have a good electrical conductivity, and prevents the reference electrode (13) from early ageing, and improves the durability of the flow-through cell unit (1).

Provided are azido-BODIPY compounds of formula (I), cyclooctyne-based fluorogenic probes of formula (IV), and activity-based probes of formula (VI). These compounds undergo azide-alkyne cycloadditions (AAC) with to form triazolyl products. The provided compounds are useful for detection and imaging of alkyne-, or azide-containing molecules. Methods for detection and imaging biomolecules using compounds of the present disclosure are disclosed.

The invention provides a method of probing for a nucleic acid comprising: contacting a nucleic acid solution with an oligonucleotide probe labelled with an electrochemically active marker, providing conditions at which the probe is able to at least partially hybridize with any complementary target sequence which may be present in the nucleic acid solution, selectively degrading either hybridized, partially hybridized or unhybridized nucleic acid probe, and electrochemically determining information relating to the electrochemically active marker. The invention further provides novel molecules with use in methods of the invention.

A method detects a target material in a sample, and includes: providing an aptasensor having a first aptamer capable of binding with hemin; a second aptamer capable of binding with the target material; and a nucleotide linker connecting the first aptamer and the second aptamer, adding hemin to the sample, contacting the aptasensor with the sample including hemin to bind the first aptamer with hemin and the second aptamer with the target material; adding a substrate for 1,1-oxalyldiimidazole (ODI) chemiluminescence (CL), and measuring intensity of CL.

Provided are dyes and compositions which are useful in a number of applications, such as the detection and monitoring protein aggregation, kinetic studies of protein aggregation, neurofibrillary plaques analysis, evaluation of protein formulation stability, and analysis of molecular chaperone activity.