The present invention provides a method of determining the cytotoxic effect of an effector molecule or cell on a target cell presenting a heterologous peptide comprising: (a) incubating said effector molecule or cell with said target cell, wherein said target cell is immobilised on a substrate comprising at least one pair of electrodes; and (b) determining the impedance during and/or after step a) wherein a decrease in impedance is indicative of target cell death. The invention further provides the use of impedance to determine the cytotoxic effect of an effector cell and/or molecule on a target cell presenting a heterologous peptide and a kit for use in the method of the invention, comprising (a) a target cell presenting a heterologous peptide or a target cell and a heterologous peptide or a nucleic acid comprising a nucleotide sequence encoding said heterologous peptide; (b) a capture molecule for immobilising said target cell to a substrate; (c) instructions for use of (a) and (b) in a method of the invention; and optionally (d) a substrate comprising at least one pair of electrodes; and/or (e) an effector molecule and/or cell which specifically binds to the heterologous peptide presented by the target cell, or an effector cell and a nucleic acid comprising a nucleotide sequence encoding a receptor which specifically binds to the heterologous peptide presented by the target cell.

Provided is an impedance measuring device for biological samples including one or a plurality of biological sample holding units configured to hold a biological sample, an applying unit configured to apply an AC voltage to a pair of electrodes in contact with the biological sample held by the biological sample holding unit, a measuring unit configured to measure an impedance of the biological sample obtained by an AC voltage being applied to the biological sample by the applying unit, and a measurement condition control unit configured to control a measuring time and/or a measuring frequency in the measuring unit.

Disclosed herein are hydrogels comprising a polynucleotide-based structural component. Methods of altering a property of a hydrogel based on user-defined nucleic acid input sequences are also disclosed. In addition, various applications are described that utilize these hydrogels and methods.

Concrete can be one of the most durable building materials and structures made of concrete can have a long service life. Consumption is projected to reach approximately 40 billion tons in 2017. Despite this the testing of concrete at all stages of its life cycle is still in its early stages although testing for corrosion is well established. Further many of the tests today are time consuming, expensive, and provide results only after it has been poured and set. Embodiments of the invention provide concrete suppliers, construction companies, regulators, architects, and others with rapid testing and performance data regarding the cure, performance, corrosion of concrete at different points in its life cycle based upon a simple electrical tests that remove subjectivity, allow for rapid assessment, are integrable to the construction process, and provided full life cycle assessment. Wireless sensors can be embedded from initial loading through post-cure into service life.

The embodiments provide a method making it possible to safely and inexpensively measure concentrations of combustible gases, such as methanol, at room temperature even in high concentration atmospheres, and also provide a sensor making it possible to carry out the above measurement method. The measurement method comprises: arranging a film containing nanoparticles of tungsten oxide and a pair of electrodes which are separated from each other and which individually keep in contact with said film in said atmosphere, exposing said film to light, measuring electric resistance change of said film before and after exposing said film to light, and determining said concentration based on said change

A method of identifying a therapeutic compound for treating cancer in a human subject, the method including: providing a device that measures cell-substrate impedance; culturing cancer cells in the at least two wells, wherein the cancer cells are obtained from a human subject and have a receptor tyrosine kinase (RTK) pathway; adding to a first well a proposed therapeutic compound that affects an RTK pathway and an RTK stimulating factor for the RTK pathway to form a test well, and adding to another well the RTK stimulating factor to form a control well; continuously monitoring cell-substrate impedance of the at least two wells; and determining a difference in impedance or optionally in cell index between the test well and control well; and if significantly different, concluding the proposed therapeutic compound is therapeutically active in the RTK pathway within the cancer cells of the human subject.

A system and method for sensing wave impedance of a material using an RF power source with a sensor structure comprised of a resonant electromagnetic radiative filter (MEF). The wave impedance is determined by processing a differential RF signal level within an interrogator comprising an impedance calculator. A differential RF signal between a source signal level and a response signal level affected by field coupling of the REF with a material of interest. In embodiments based on frequency scanning transmissometry (FST), the impedance spectrometer determines both the real and imaginary part of the wave impedance of the material. In embodiments the impedance spectrometer comprises an RFID transponder. In embodiments, the interrogator is disposed as payload on a UAV drone. In embodiments, the impedance spectrometer is a node within a communications network.

Embodiments herein include a kinetic response system for measuring analyte presence on a chemical sensor element. The chemical sensor element includes one or more discrete binding detectors, each discrete binding detector including a graphene varactor. The kinetic response system includes a measurement circuit having an excitation voltage generator for generating a series of excitation cycles over a time period. Each excitation cycle includes delivering a DC bias voltage to the discrete binding detectors at multiple discrete DC bias voltages across a range of DC bias voltages. The kinetic response system includes a capacitance sensor to measure capacitance of the discrete binding detectors resulting from the excitation cycles. The kinetic response system includes a controller circuit to determine the kinetics of change in at least one of a measured capacitance value and a calculated value based on the measured capacitance over the time period. Other embodiments are also included herein.

A system and method for sensing wave impedance of a material using an RF power source with a sensor structure comprised of a resonant electromagnetic radiative filter (MEF). The wave impedance is determined by processing a differential RF signal level within an interrogator comprising an impedance calculator. A differential RF signal between a source signal level and a response signal level affected by field coupling of the REF with a material of interest. In embodiments based on frequency scanning transmissometry (FST), the impedance spectrometer determines both the real and imaginary part of the wave impedance of the material. In embodiments the impedance spectrometer comprises an RFID transponder. In embodiments, the interrogator is disposed as payload on a UAV drone. In embodiments, the impedance spectrometer is a node within a communications network.

A system and method for antibiotic susceptibility testing efficiently determines whether bacteria are alive or have been killed by antibiotic treatment. The antibiotic susceptibility testing device includes at least one reservoir into which a bacteria solution is introduced and a microfluidic channel connected to the reservoir, wherein the cross-sectional size of the microfluidic channel is selected to be comparable to the size of the bacterium to be tested. Furthermore, the electrical resistance or voltage signal across the microchannel is monitored as bacteria swim into and out of the channel. Alternatively, a small population of bacteria can be immobilized in the microchannel. The resistance or voltage signal fluctuates when the bacteria are alive and moving in and out of the channel or wiggling on the microchannel walls. If the bacteria are dead, they have limited motility and the signal fluctuations are significantly smaller. By monitoring the signal fluctuations, the antibiotic susceptibility testing device can determine whether or not bacteria are alive, thus enabling antibiotic susceptibility testing of bacteria.