Development chambers suitable for use in thin layer chromatography are disclosed. Methods of making and using development chambers in thin layer chromatography are also disclosed.

Development chambers suitable for use in thin layer chromatography are disclosed. Methods of making and using development chambers in thin layer chromatography are also disclosed.

A method for simulataneously separating and detecting aldehydes or ketones from a plurality of samples containing the samein a simple and rapid manner by using a rotary microdevice capable of integrating derivatization and TLC separation of aldehydes and ketones, and the method providing reliable TLC separation, control of moving speed of an eluent on a TLC plate, and improved TLC resolution.

A method for simulataneously separating and detecting aldehydes or ketones from a plurality of samples containing the samein a simple and rapid manner by using a rotary microdevice capable of integrating derivatization and TLC separation of aldehydes and ketones, and the method providing reliable TLC separation, control of moving speed of an eluent on a TLC plate, and improved TLC resolution.

A method for screening adulteration of fibrate anti-hyperlipidemia chemicals in tea by combined method of high performance thin layer chromatography (HPTLC) and bioluminescence, which belongs to the field of food inspection. The method includes: firstly, formulating standard solutions of bezafibrate and ciprofibrate, and preparing a tea sample; pre-washing a thin-layer plate and then performing HPTLC spotting; performing HPTLC separation to move original mixed target objects mixed originally onto different positions of the thin-layer plate according to different molecular structures, so as to form a physical isolation; and subsequently, simultaneous detection of multiple targets in the tea sample could be realized conveniently through luminous bacteria coupled with the thin-layer plate by an immersed manner. The present disclosure establishes a method capable of detecting anti-hyperlipidemia chemicals in tea rapidly and quantitatively by the combined detection method of HPTLC and bioluminescence, which has the advantages of being economic, rapid, simple and convenient.

A thin-layer chromatography development chamber (1) that can be used for developing thin-layer chromatography plates (21) by introducing a liquid solvent onto a sample arranged on the surface of a thin-layer chromatography plate (21), whereby the solvent and the thin-layer chromatography plate (21) can be inserted into the development chamber (1), comprises a housing (2) with an opening (3). The opening (3) of the development chamber (1) can be closed with a cover (4) that comprises a data input unit (10) for entering or collecting data related to a development process performed within the development chamber (1). The cover (4) further comprises a data communication unit (16) that enables a wireless data transmission of the data related to the development process to a thin-layer chromatography data storage device (24).

A thin-layer chromatography development chamber (1) that can be used for developing thin-layer chromatography plates (21) by introducing a liquid solvent onto a sample arranged on the surface of a thin-layer chromatography plate (21), whereby the solvent and the thin-layer chromatography plate (21) can be inserted into the development chamber (1), comprises a housing (2) with an opening (3). The opening (3) of the development chamber (1) can be closed with a cover (4) that comprises a data input unit (10) for entering or collecting data related to a development process performed within the development chamber (1). The cover (4) further comprises a data communication unit (16) that enables a wireless data transmission of the data related to the development process to a thin-layer chromatography data storage device (24).

The present invention relates to a method for simultaneously qualitatively and quantitatively analyzing compounds of aldehydes and/or ketones in a short time by using an optimal TLC plate, a proportion of a developing solvent, a sample amount, etc., and can provide an analysis result equivalent to a conventional analysis result in a shorter time by providing the most optimal conditions when using the TLC method.

The present invention relates to a method for simultaneously qualitatively and quantitatively analyzing compounds of aldehydes and/or ketones in a short time by using an optimal TLC plate, a proportion of a developing solvent, a sample amount, etc., and can provide an analysis result equivalent to a conventional analysis result in a shorter time by providing the most optimal conditions when using the TLC method.

A method is provided for preparing a sample of a specimen to be analysed from a thin-layer plate in thin-layer chromatography. The sample is removed from a surface of the thin-layer plate by means of a blade disposed at a receiving opening of a cannula and fed through the cannula to a specimen chamber connected to the cannula. A reduced pressure can be generated in the specimen chamber to suck the sample removed from the thin-layer plate by means of the cannula through the receiving opening into and through the cannula and into the specimen chamber. After removal and feed of the sample, the specimen chamber can be filled with a solvent to dissolve the sample. The cannula with the blade can be deflected against a spring force or the blade can be deflected against a spring force relative to the receiving opening of the cannula at the receiving opening of the cannula to prevent the blade from penetrating into the thin-layer plate with an excessive contact pressure during the removal of the sample from the thin-layer plate.