This invention is in the field of homeostasis analysis. More particularly, it relates to systems and methods for analyzing persistent homeostatic perturbations, i.e., chronic stress, by measuring levels of biomarkers that are related to chronic stress. This invention is also directed to systems and methods for analyzing the molecular mechanisms of chronic stress.

The present invention generally relates to in vitro methods for mimicking in vivo pathological or physiologic conditions. The methods comprise applying shear forces to a cell type or cell type plated on a surface within a cell culture container. Methods for testing drugs or compounds in such systems are also described.

The present invention relates to a method for mimicking the effects of ionizing radiations on cells, wherein cells are contacted with bleomycin.

Disclosed are methods for assessing severity, determining future risk, matching with a drug treatment, and measuring response to treatment, for stress disorders. Also disclosed are new methods of use for drugs and natural compounds repurposed for use in reducing stress severity, as well as for preventing and treating stress disorders. All the above mentioned methods are computer assisted methods analyzing the expression of panels of genes, clinical measures, and drug databases. A universal approach in everybody, as well as a personalized approaches by gender, and by diagnosis, are disclosed.

The present invention generally relates to in vitro methods for mimicking in vivo pathological or physiologic conditions. The methods comprise applying shear forces to a cell type or cell type plated on a surface within a cell culture container. Methods for testing drugs or compounds in such systems are also described.

Provided herein are methods and devices for the detection of conditions or disorders by detecting altered levels of stress response pathway biomarkers. Also provided are methods and reagents for identifying panels of biomarkers associated with a condition or disorder.

The present disclosure relates to methods, kits and systems for assessing cytotoxicity of immunoresponsive cells using a pleural effusion. The present disclosure also relates to methods, kits and systems for assessing effect of an immunotherapeutic agent on cytotoxicity of immunoresponsive cells using a pleural effusion.

Provided herein are kits, compositions and methods for biomarker detection, and the use of relative or absolute levels of these markers for non-invasive detection of stress and/or relaxation levels in human subjects. In particular, the present invention relates to cortisol, isomer and metabolite markers for stress and/or relaxation levels.

Provided herein are methods and devices for the detection of conditions or disorders by detecting altered levels of stress response pathway biomarkers. Also provided are methods and reagents for identifying panels of biontarkers associated with condition or disorder.

Disclosed herein are nucleic acids comprising reporter constructs for detecting cellular signaling changes in a stressed cell. Also provided are methods for detecting cellular signaling changes in cells undergoing stress, as well as vectors and cells comprising nucleic acids comprising reporter constructs for detecting cellular signaling changes in a stressed cell.