A method of coating a surface with nanoparticles for biological analysis of cells that includes the steps of cleaning the surface with an oxidizing acid, treating the surface with an organosilane, coating the surface with nanoparticles, and then growing cells on the surface coated with the nanoparticles. The surface may be a glass surface, a silica-based surface, a plastic-based surface or a polymer-based surface. The nanoparticles may be gold-based nanomaterials.

The present invention relates to a method of preserving cells in a cell preservative solution. The cell preservative solution comprises a lower alcohol having 1 to 6 carbon atoms and a divalent metal ion in an aqueous solvent, wherein the concentration of the divalent metal ion is from about 6 mmol/L to about 82 mmol/L.

Devices and methods for capturing and imaging a specimen, such as a living organism, are disclosed. In some embodiments, a method for imaging a living organism in vivo includes placing at least one specimen into a hydrogel polymer on a substrate, and curing the hydrogel to embed the at least one specimen within the cured hydrogel such that the at least one specimen is kept alive and the movement of the at least one specimen within the cured hydrogel is restricted. The at least one embedded specimen can be imaged.

The disclosure relates to devices, solutions and methods for collecting and processing samples of bodily fluids containing cells (as well as embodiments for the collection, and processing and/or analysis of other fluids including toxic and/or hazardous substances/fluids). In addition, the disclosure relates generally to function genomic studies and to the isolation and preservation of cells from saliva and other bodily fluids (e.g., urine), for cellular analysis. With respect to devices for collection of bodily fluids, some embodiments include two mating bodies, a cap and a tube (for example), where, in some embodiments, the cap includes a closed interior space for holding a sample preservative solution and mates with the tube to constitute the (closed) sample collection device. Upon mating, the preservation solution flows into the closed interior space to preserve cells in the bodily fluid. The tube is configured to receive a donor sample of bodily fluid (e.g., saliva, urine), which can then be subjected to processing to extract a plurality of cells. The plurality of cells can be further processed to isolate one and/or another cell type therefrom. The plurality of cells, as well as the isolated cell type(s), can be analyzed for functional genomic and epigenetic studies, as well as biomarker discovery.

The present invention relates to a method for three-dimensional nucleic acid imaging diagnosis of a tissue by using isothermal nucleic acid amplification. The method for three-dimensional nucleic acid imaging diagnosis of tissue according to the present invention can allow a specific molecular biomarker to be clearly seen in tissue through clearing of the tissue, enhance diagnostic accuracy by three-dimensionally reconstituting the tissue since all of the inside of the tissue is visualized, and facilitate three-dimensional imaging of a molecular biomarker such as DNA or RNA containing genetic information in the human body through isothermal nucleic acid amplification in tissue, and thus this method can be effectively used in diagnosis of various diseases including cancer.

The present invention is directed to a mass spectrometry approach to identifying non-Spitzoid melanoma, and distinguishing non-Spitzoid nevi from non-Spitzoid malignant melanoma.

The present invention relates to a method for the treatment of a biological material, comprising the steps i) providing a biological material, and ii) contacting the biological material with a first non-aqueous composition comprising: (a1) 10 to 90 vol. % methanol, and (a2) at least one additional additive, and (a3) optionally an acid. iii) transferring the biological material into a second composition (B) comprising up to 99 vol. % ethanol as well as to a new composition for preservation of a biological material usable in said method and the biological material resulting from this method, a method for the analysis of a treated biological material, various kits as well as the use of the composition in such a method.

The present invention relates to specific complexes comprising heavy metal ions having an atomic number of 29 or higher and 83 or lower (preferably 29 or higher and 81 or lower) and one or more ligand(s) selected from the group consisting of specific xanthene derivatives, preferably eosin Y and/or erythrosin B ligand(s). In particular, the invention relates to the use of the complexes as ex vivo contrast agents for a computed tomography scanning of a biological sample. Moreover, the invention relates to specific ex vivo methods for investigating a biological sample by means of computed tomography scanning methods, wherein the method comprises staining the biological sample with a solution comprising one or more of the complex(es); or wherein the method comprises staining the biological sample with a staining solution comprising one or more specific xanthenes derivatives (e.g. eosin Y and/or erythrosin B), and separately contacting the biological sample with one or more staining solution(s) comprising one or more heavy metal ions having an atomic number of 29 or higher and 83 or lower (preferably 29 or higher and 81 or lower).

Disclosed are a composition for clearing spheroids, a method for clearing spheroids using same, and a kit comprising same. The composition for clearing spheroids can clear the spheroids in a convenient and rapid manner and thus may be usefully used for imaging of spheroids and in identifying the causes of various diseases, treating the diseases, and predicting the therapeutic effects and toxicity of drugs. In addition, the composition may be used in combination with various medical devices, and in particular, can be prepared as a kit and usefully used as an in vitro medical diagnostic device.

The present invention relates to an immunoassay method and an immunoassay device.

According to an aspect of the present invention, provided is an immunoassay device including: a stage capable of accommodating a plurality of cartridges having a plurality of wells; a solution transfer unit including a plurality of tips, which are movable relative to the stage and disposed to correspond to a position of the cartridge so as to suction a solution stored in each of the wells or discharge the suctioned solution from the well; and a control unit controlling the stage and the solution transfer unit to be relatively movable and controlling suction or discharge of a content into/from the plurality of tips.