Electrochemical sensors can include at least two electrodes, over which an electrolyte is formed. The electrodes can be isolated from one another in order for reduction/oxidation reactions to occur at the electrodes and for an electric current to flow therebetween. The present disclosure describes the use of a barrier in the electrochemical sensor that is configured to isolate electrodes from one another for the purpose of preventing electrode shorting. Additionally, the physical structure of the barrier can also act as a stencil for shaping the electrodes.

Electrochemical sensors can include at least two electrodes, over which an electrolyte is formed. The electrodes can be isolated from one another in order for reduction/oxidation reactions to occur at the electrodes and for an electric current to flow therebetween. The present disclosure describes the use of a barrier in the electrochemical sensor that is configured to isolate electrodes from one another for the purpose of preventing electrode shorting. Additionally, the physical structure of the barrier can also act as a stencil for shaping the electrodes.

A electrochemical sensor (100) for sensing an analyte in an associated volume (106), the sensor comprising a first solid element (126), a second solid element (128) being joined to the first solid element, a chamber (110) being placed at least partially between the first solid element and the second solid element, a working electrode (104) in the chamber (110) and wherein one or more analyte permeable openings (122) connect the chamber with the associated volume (106) and wherein the electrochemical sensor (100) further comprises an analyte permeable membrane (124) in said one or more analyte permeable openings, and wherein the one or more analyte permeable openings are arranged so that a distance from any point in at least one cross-sectional plane to the nearest point of a wall of said opening is 25 micrometer or less.

A electrochemical sensor (100) for sensing an analyte in an associated volume (106), the sensor comprising a first solid element (126), a second solid element (128) being joined to the first solid element, a chamber (110) being placed at least partially between the first solid element and the second solid element, a working electrode (104) in the chamber (110) and wherein one or more analyte permeable openings (122) connect the chamber with the associated volume (106) and wherein the electrochemical sensor (100) further comprises an analyte permeable membrane (124) in said one or more analyte permeable openings, and wherein the one or more analyte permeable openings are arranged so that a distance from any point in at least one cross-sectional plane to the nearest point of a wall of said opening is 25 micrometer or less.

Devices and methods for measuring phosphate levels in a fluid, such as natural water, are disclosed. The devices and methods rely upon the anodic dissolution of molybdenum to generate a reagent from a phosphate-containing fluid, which is then measured electrochemically to determine a level of phosphate ions in the fluid. The different embodiments of devices used for performing this technique are transportable and have extended lifetimes when compared to existing devices used to measure phosphate levels in fluid. They can be used in situ at the source of a site to generate results within about two minutes, within about thirty seconds, and even within about ten seconds. They also consume much less energy and molybdenum per measurement. The disclosed embodiments include devices having one or two molybdenum electrodes, with one of the electrodes disposed near a working electrode. Various methods for determining phosphate levels in fluids are also provided.

Devices and methods for measuring phosphate levels in a fluid, such as natural water, are disclosed. The devices and methods rely upon the anodic dissolution of molybdenum to generate a reagent from a phosphate-containing fluid, which is then measured electrochemically to determine a level of phosphate ions in the fluid. The different embodiments of devices used for performing this technique are transportable and have extended lifetimes when compared to existing devices used to measure phosphate levels in fluid. They can be used in situ at the source of a site to generate results within about two minutes, within about thirty seconds, and even within about ten seconds. They also consume much less energy and molybdenum per measurement. The disclosed embodiments include devices having one or two molybdenum electrodes, with one of the electrodes disposed near a working electrode. Various methods for determining phosphate levels in fluids are also provided.

Electrochemical sensors (100) include at least two electrodes (110A, HOB), over which an electrolyte (114) is formed. The electrodes are isolated from one another in order for reduction/oxidation reactions to occur at the electrodes and for an electric current to flow therebetween. The present disclosure describes the use of a barrier (121) in the electrochemical sensor that is configured to isolate electrodes from one another for the purpose of preventing electrode shorting. Additionally, the physical structure of the barrier can also act as a stencil for shaping the electrodes.

Electrochemical sensors (100) include at least two electrodes (110A, HOB), over which an electrolyte (114) is formed. The electrodes are isolated from one another in order for reduction/oxidation reactions to occur at the electrodes and for an electric current to flow therebetween. The present disclosure describes the use of a barrier (121) in the electrochemical sensor that is configured to isolate electrodes from one another for the purpose of preventing electrode shorting. Additionally, the physical structure of the barrier can also act as a stencil for shaping the electrodes.

Methods, apparatuses and systems for providing gas detecting apparatuses (e.g., electrochemical detectors) are disclosed herein. An example gas detecting apparatus may comprise a sensing component comprising: a first sensing electrode configured to generate a first concentration level indication associated with a first portion of a sample gaseous substance disposed within the sensing component; and a second sensing electrode operatively coupled to a filtering element that is configured to absorb at least one substance from the sample gaseous substance, wherein the second sensing electrode is configured to generate a second concentration level indication associated with a second portion of the sample gaseous substance.

The invention relates to a system (100) for monitoring deviations of a state of a cell culture in a bioreactor (104, 106) from a reference state of a cell culture in a reference bioreactor (102). The bioreactor comprises the same medium (M1) as the reference bioreactor. The system comprises: •—a storage medium (114) comprising: •a PACO-reference profile (116) indicative of a deviation of a CO2 off gas rate (ACO.sub.R-M-.sub.ti) measured in the reference bioreactor from a predicted CO2 off gas rate (ACO.sub.R-EXP-ti) of the reference bioreactor; •a data object comprising a medium-specific relation (136) between the pH value of the medium (M1) and a respective fraction of CO2 gas in a gas volume when said medium is in pH-CO2 equilibrium state with said gas volume and lacks the cell culture; •—an interface (128) for receiving (212) a current CO2 off gas rate (ACO.sub.Bi-M-ti, ACO.sub.B2-M-.sub.t i) and a current pH value (pH.sub.Bi-ti) of the medium of the bioreactor (104, 106); •—a comparison unit (130) configured for computing (214, 216): •a PACO value (PACO.sub.B1-tir PACO.sub.Bi-ti) the PACO-value being indicative of a deviation of a CO2 off gas rate (ACO.sub.Bi-M-ti, ACO.sub.B2-M-.sub.ti) measured in the bioreactor from a predicted CO2 off gas rate (ACO.sub.B1-EXP-ti, ACO.sub.B2-E xp-.sub.t i). a difference between the computed PACO value (PACO.sub.Bi-ti, PACO.sub.B2-ti) and a respective reference PACO value (PACO.sub.R-ti) in the PACO-reference profile (116).