The invention belongs to the technical field of natural medicinal chemistry and quality control thereof, and relates to a method for determining the weight average molecular weight and the purity of a soluble salt of an acidic saccharide. The method comprises using metal ion content in the soluble salt of an acidic saccharide to correct the weight average molecular weight and the content of the of acid saccharide obtained by the combined use of the molecular sieve chromatography and a multi-angle laser scattering detector SEC-MALS. The method of the present invention can be used to more quickly and accurately determine the weight average molecular weight and content of acidic saccharide soluble salts.

The present disclosure relates to a marker for identifying Korean ginseng cultivars and a method of identifying Korean ginseng cultivars using the same. Specifically, the present disclosure provides a marker for identification of Korean ginseng cultivars, which is obtained by identifying and separating an active ingredient, which is contained only in Korean ginseng cultivars, among non-saponin components. In addition, the present disclosure makes it possible to determine whether a product sold as Korean ginseng is Korean ginseng by separating and purifying the active ingredient without using a conventional molecular biological method.

The present disclosure relates to a marker for identifying Korean ginseng cultivars and a method of identifying Korean ginseng cultivars using the same. Specifically, the present disclosure provides a marker for identification of Korean ginseng cultivars, which is obtained by identifying and separating an active ingredient, which is contained only in Korean ginseng cultivars, among non-saponin components. In addition, the present disclosure makes it possible to determine whether a product sold as Korean ginseng is Korean ginseng by separating and purifying the active ingredient without using a conventional molecular biological method.

A main controller 201 of a main substrate 20 performs serial communications with a sub controller 214 of each flowrate control substrate 21. The flowrate of the carrier gas is controlled with the flowrate control circuit 213 under the control performed by each sub controller 214. Thus, the main controller 201 only needs to execute the processing of performing the serial communications with each sub controller 214. As a result, the processing executed by the main controller 201 can be reduced, and the processing executed by the main controller 201 is less likely to overwhelm its processing capability even when the number of flowrate control substrates 21 is increased. In addition, a signal line 40 between the main controller 201 and each sub controller 214 can be made long. Thus, the distance between the main substrate 20 and each of the flowrate control substrates 21 can be made long.

A device and a method is provided for profiling particles such as proteins. The device comprises: a liquid chromatography column (16) in a mixture separation module (10); a fractionation device (22, 24) and a plurality of microfluidic analysis modules (26, 28) in a microfluidic network (14). The microfluidic analysis modules are configured to provide multi-dimensional analysis of the particles. Furthermore, a fluidic flow adaptor (20) allows for controlled flow between separator (16) and the microfluidic network to provide a continuous fluid flow.

A device and a method is provided for profiling particles such as proteins. The device comprises: a liquid chromatography column (16) in a mixture separation module (10); a fractionation device (22, 24) and a plurality of microfluidic analysis modules (26, 28) in a microfluidic network (14). The microfluidic analysis modules are configured to provide multi-dimensional analysis of the particles. Furthermore, a fluidic flow adaptor (20) allows for controlled flow between separator (16) and the microfluidic network to provide a continuous fluid flow.

A multivariate analysis operation unit (43) represents each of chromatogram data at a specific wavelength λ1 in data acquired by a PDA detector (2) and mass spectrum data repeatedly obtained by a mass spectrometer (3) in the form of a matrix, and then calculates a regression coefficient matrix by performing a PLS operation with a two-dimensional matrix based on the mass spectrum data as an explanatory variable and a one-dimensional matrix based on the chromatogram data as an explained variable. A regression coefficient is obtained with respect to each m/z value, and an m/z value having a high regression coefficient indicates an m/z value of which the chromatogram wavelength at a specific wavelength is similar to an extracted ion chromatogram (XIC). Accordingly, an m/z-value extracting unit (44) compares the regression coefficient with a threshold and extracts a significant m/z value, and an XIC creating unit (46) creates an XIC of the extracted m/z value. By specifying the wavelength λ1 that an operator's target partial chemical structure specifically absorbs, an XIC corresponding to a molecular species containing the partial chemical structure can be obtained without waveform processing manually performed by the operator.

A multivariate analysis operation unit (43) represents each of chromatogram data at a specific wavelength λ1 in data acquired by a PDA detector (2) and mass spectrum data repeatedly obtained by a mass spectrometer (3) in the form of a matrix, and then calculates a regression coefficient matrix by performing a PLS operation with a two-dimensional matrix based on the mass spectrum data as an explanatory variable and a one-dimensional matrix based on the chromatogram data as an explained variable. A regression coefficient is obtained with respect to each m/z value, and an m/z value having a high regression coefficient indicates an m/z value of which the chromatogram wavelength at a specific wavelength is similar to an extracted ion chromatogram (XIC). Accordingly, an m/z-value extracting unit (44) compares the regression coefficient with a threshold and extracts a significant m/z value, and an XIC creating unit (46) creates an XIC of the extracted m/z value. By specifying the wavelength λ1 that an operator's target partial chemical structure specifically absorbs, an XIC corresponding to a molecular species containing the partial chemical structure can be obtained without waveform processing manually performed by the operator.

A flow cell for a detector in a liquid chromatography system includes a flow cell body, an inlet, an outlet, at least two windows situated on opposing sides of the flow cell body, which are transparent for light, and at least two electrodes configured for a conductivity measurement. A detection channel is formed within said flow cell body, fluidly connecting said inlet and outlet, includes an optical path situated in between said two windows such that a light absorption measurement may be performed for a liquid passing through the detection channel, and a conductivity path formed by at least partially drilling through said electrodes to allow for a physical contact of a liquid passing through the detection channel. A detector or liquid chromatography system including such a flow cell as well as uses of the flow cell, detector or liquid chromatography system are also described.

A system comprises: first and second mass spectrometers; at least one liquid chromatograph configured to simultaneously supply a first stream of chromatographic eluate derived from a sample to the first mass spectrometer and a second stream of chromatographic eluate to the second mass spectrometer; and a computer or electronic controller electronically coupled to both of the first and second mass spectrometers and comprising computer-readable instructions operable to: input a mass spectrometric analysis of a chromatographic fraction of the sample obtained by the first mass spectrometer; determine whether an additional mass spectrometric analysis of the chromatographic fraction of the sample is required, based on the mass spectrometric analysis of the chromatographic fraction obtained by the first mass spectrometer; and, if the determination is affirmative, cause the second mass spectrometer to perform, after a time delay, the additional mass spectrometric analysis of the chromatographic fraction of the sample.