The present disclosure describes an apparatus, method, and system of measuring attributes of a viral gene delivery vehicle sample via separation. In an embodiment, the method, system, and computer program product include executing a set of logical operations analyzing a viral gene delivery vehicle sample on a set of analytical instruments, where the set includes at least one separation instrument, at least one static light scattering instrument, and at least two concentration detectors, resulting in a capsid protein mass of the sample, m.sub.A, a modifier mass of the sample, m.sub.B, and a modifier molar mass of the sample, M.sub.B, receiving a capsid protein molar mass of the sample, M.sub.A, from a capsid protein molar mass data source, receiving an injection volume of the sample, v, from an injection volume data source, and executing a set of logical operations calculating a total VGDV particle concentration of the sample, C.sub.A.

An apparatus chemical analysis of a sample includes: a chromatographic module configured to separate compounds that make up a mixture to be analyzed and which is configured to determine a linear retention index of the separated compounds; a module for mass spectrometry, configured to detect and/or determine mass spectra of the separated compounds, from the chromatographic module; a module for IR spectroscopy, configured to detect and/or determine the IR spectra of the separated compounds, from said chromatographic module; at least one processing unit configured to receive data, acquired by the chromatographic module; the module for mass spectrometry; and the module for IR spectroscopy; and at least one memory unit associated with the at least one processing unit, having at least one organized file containing the retention indices, the mass and IR spectra of a plurality of known and predefined compounds.

An apparatus chemical analysis of a sample includes: a chromatographic module configured to separate compounds that make up a mixture to be analyzed and which is configured to determine a linear retention index of the separated compounds; a module for mass spectrometry, configured to detect and/or determine mass spectra of the separated compounds, from the chromatographic module; a module for IR spectroscopy, configured to detect and/or determine the IR spectra of the separated compounds, from said chromatographic module; at least one processing unit configured to receive data, acquired by the chromatographic module; the module for mass spectrometry; and the module for IR spectroscopy; and at least one memory unit associated with the at least one processing unit, having at least one organized file containing the retention indices, the mass and IR spectra of a plurality of known and predefined compounds.

Automated systems and methods for obtaining of the concentration of impurities in a liquid ethylene oxide product stream are shown and described. The systems and methods employ remote injection and flash vaporization of small volumes of liquid ethylene oxide into a carrier gas to minimize polymerization of the ethylene oxide and accumulation of polymerized ethylene oxide. Ethylene oxide peaks are diverted from the gas chromatograph effluent detector to stabilize baseline signal errors and avoid errors in the calculation of an impurity with an adjacent retention time peak. The systems and methods may be used for feedback, feedforward, dynamic matrix, and/or model-based predictive control of ethylene oxide purity. The systems and methods reduce lag times and errors associated with relying on laboratory analyses to make process adjustments.

Automated systems and methods for obtaining of the concentration of impurities in a liquid ethylene oxide product stream are shown and described. The systems and methods employ remote injection and flash vaporization of small volumes of liquid ethylene oxide into a carrier gas to minimize polymerization of the ethylene oxide and accumulation of polymerized ethylene oxide. Ethylene oxide peaks are diverted from the gas chromatograph effluent detector to stabilize baseline signal errors and avoid errors in the calculation of an impurity with an adjacent retention time peak. The systems and methods may be used for feedback, feedforward, dynamic matrix, and/or model-based predictive control of ethylene oxide purity. The systems and methods reduce lag times and errors associated with relying on laboratory analyses to make process adjustments.

The invention belongs to the technical field of natural medicinal chemistry and quality control thereof, and relates to a method for determining the weight average molecular weight and the purity of a soluble salt of an acidic saccharide. The method comprises using metal ion content in the soluble salt of an acidic saccharide to correct the weight average molecular weight and the content of the of acid saccharide obtained by the combined use of the molecular sieve chromatography and a multi-angle laser scattering detector SEC-MALS. The method of the present invention can be used to more quickly and accurately determine the weight average molecular weight and content of acidic saccharide soluble salts.

The invention relates to a method for analysing hydrocarbons, comprising: the implementation of a gas chromatography separation according to a first controlled temperature profile, to separate a sample into a plurality of analytes; the detection of at least one of said analytes by measurement of a variation of the resonance frequency of at least one resonator of nano-electromechanical system (NEMS) type covered with a functional layer made to vibrate at the resonance frequency thereof, under the effect of an adsorption or desorption of the analyte by the functional layer, said method being characterised in that the resonator is subjected to a second controlled temperature profile, lower than the first profile.

The invention relates to a method for analysing hydrocarbons, comprising: the implementation of a gas chromatography separation according to a first controlled temperature profile, to separate a sample into a plurality of analytes; the detection of at least one of said analytes by measurement of a variation of the resonance frequency of at least one resonator of nano-electromechanical system (NEMS) type covered with a functional layer made to vibrate at the resonance frequency thereof, under the effect of an adsorption or desorption of the analyte by the functional layer, said method being characterised in that the resonator is subjected to a second controlled temperature profile, lower than the first profile.

In order to achieve a system capable of analyzing a wide range of compounds while saving time and energy consumption, a progressive cellular architecture is presented for vapor collection and gas chromatographic separation. Each cell includes a preconcentrator and separation column that are adapted for collecting and separating compounds only within a specific volatility range. A wide volatility range can therefore be covered by the use of multiple cells that are cascaded in the appropriate order. The separation columns within each cell are short enough to reduce the heating and pumping requirements. The gas flow for vapor collection and separation is provided by low-power gas micropumps that use ambient air. The system is also configurable to incorporate capabilities of detecting and reducing vapor overload. The progressive cellular architecture directly address the compromise between low power and broad chemical analyses.

In order to achieve a system capable of analyzing a wide range of compounds while saving time and energy consumption, a progressive cellular architecture is presented for vapor collection and gas chromatographic separation. Each cell includes a preconcentrator and separation column that are adapted for collecting and separating compounds only within a specific volatility range. A wide volatility range can therefore be covered by the use of multiple cells that are cascaded in the appropriate order. The separation columns within each cell are short enough to reduce the heating and pumping requirements. The gas flow for vapor collection and separation is provided by low-power gas micropumps that use ambient air. The system is also configurable to incorporate capabilities of detecting and reducing vapor overload. The progressive cellular architecture directly address the compromise between low power and broad chemical analyses.