A method of assessing a bodily fluid sample on a test strip may involve applying a periodic signal with a first electrode located at a first location in a microfluidic channel of the test strip, monitoring the applied periodic signal with a second electrode located at a second location in the microfluidic channel, and using a third electrode located at a third location in the microfluidic channel as a reference electrode. The method may also include: collecting the bodily fluid sample in the microfluidic channel; continuing to apply the periodic signal, monitor the periodic signal and use the third electrode as a reference electrode while collecting the bodily fluid sample; and determining that the bodily fluid sample is sufficient for analyzing, based at least in part on the applied and monitored periodic signal.

In one example in accordance with the present disclosure, a cell preparation system is described. The cell preparation system includes a fluidic channel to transport cells in single file past multiple detection devices. The cell preparation system also includes a series of detection devices. Each detection device includes 1) a constriction to deform a cell found therein and 2) a sensor to measure a state within the constriction. The cell preparation system also includes a controller. The controller analyzes outputs from multiple sensors to 1) verify a single file transport of cells through the system and 2) identify a cell that passes through the fluidic channel.

The present invention provides a nanopore single-molecule protein sequencer, which mainly includes a nanopore array chip system, an ultra-low current detection system and a data processing and construction system; the chip mainly includes a chip of peptide charge screening, a chip of amino acid sequence reading based on a series of specific nanopores and the like, the design principle of which is as follows: a series of biological nanopores with amino acid targeted identification are designed according to properties such as hydrophilicity and hydrophobicity, polarity, and chargeability of amino acids, and characteristic ion flow signals of amino acids forming a protein to be detected in nanopore are obtained one by one; characteristic information of a protein sequence in each of the nanopores is acquired by an arrayed ultra-low current measurement system; a standard model peptide sequence information base is used for identifying, correcting, integrating and reading amino acid sequences.

A portable detector is disclosed for detecting certain analytes of interest, such as genetic material (e.g., nucleic acids). The detector includes a reading component for the detection of the analytes, and control circuitry for controlling operation of the reading component. Processing circuitry may be included to perform both primary analysis of acquired data, and where desired, secondary analysis. Where desired, some or all of the computationally intensive tasks may be off-loaded to enhance the portability and speed of the device. The device may incorporate various types of interface, technologies for reading and analysis, positioning system interfaces, and so forth. A number of exemplary use cases and methods are also disclosed.

The invention relates to a new method of characterising a target RNA polynucleotide by taking one or more measurements as the target RNA polynucleotide moves with respect to a transmembrane pore. The movement is controlled by a DNA helicase. The invention also relates to a modified RNA construct wherein the RNA polynucleotide has been modified to increase DNA helicase binding thereto.

Provided herein are methods and devices for characterizing a biomolecule parameter by a nanopore-containing membrane, and also methods for making devices that can be used in the methods and devices provided herein. The nanopore membrane is a multilayer stack of conducting layers and dielectric layers, wherein an embedded conducting layer or conducting layer gates provides well-controlled and measurable electric fields in and around the nanopore through which the biomolecule translocates. In an aspect, the conducting layer is graphene.

Examples herein provide a device. The device includes a sample delivery component, which includes: a reagent chamber to contain at least one reagent; a sample chamber to contain a fluid sample; and a delivery channel extending from the reagent chamber and in fluid communication with the sample chamber and an output port, wherein the delivery channel is conducive mixing the at least one reagent and the fluid sample to form a mixture before the mixture reaches the output port and be discharged therefrom. The device includes a testing cassette detachable from the delivery component, which includes: an input port in fluid communication with a microfluidic reservoir, the input port to receive the discharged fluid sample from the output port; and a micro-fabricated integrated sensor in a microfluidic channel extending from the microfluidic reservoir.

Provided herein are devices and methods for analysis of male fertility. The invention provides self-contained, hand-held receptacles and systems for collection and analysis of semen samples and methods of using such devices to analyze semen samples. Also provided are processor-implemented and machine learning methods of analyzing semen sample data obtained using devices of the invention.

Provided herein are devices and methods for analysis of male fertility. The invention provides self-contained, hand-held receptacles and systems for collection and analysis of semen samples and methods of using such devices to analyze semen samples. Also provided are processor-implemented and machine learning methods of analyzing semen sample data obtained using devices of the invention.

A method to determine the throughput speed v of a pore, comprising the steps of feeding, by means of a driving force F, a filiform calibration element through the pore, the calibration element having a plurality of markers spaced apart by known distances and configured to produce an interaction event that transmits a signal away from the pore upon interaction with the pore, detecting a plurality of interaction events, and determining a time interval Δt between successive interaction events, and/or a frequency ω of interaction events.