A system and method of isolating extracellular vesicles. The method includes loading one or more of blood or bone marrow into an input port of a concentration system and centrifuging one or more of the blood or bone marrow to separate one or more of red blood cells, platelet poor plasma, or platelet rich plasma/bone marrow concentrate fractions via a centrifuge device. The method further includes pumping one or more of bone marrow/platelet rich plasma fractions and platelet poor plasma fractions into a receptacle of the concentration system and adding a concentrated aqueous two-phase solution to one or more of the bone marrow concentrate/platelet rich plasma fractions and platelet poor plasma fractions. The method also includes drawing the concentrated aqueous two-phase solution and one or more of the bone marrow concentrate/platelet rich plasma fractions or platelet poor plasma fractions back into the centrifuge device to isolate one or more of extracellular vesicles and platelet rich plasma/bone marrow concentrate fractions.

A method is provided for removing red blood cells from a suspension comprising red blood cells, white blood cells, platelets and plasma using a spinning membrane separator. The method comprises: a) flowing whole blood into the gap of the spinning membrane separator; b) collecting red blood cells and white blood cells in the gap and passing plasma and platelets through the membrane; c) introducing a first quantity of lysing buffer into the gap; d) incubating the red blood cells, white blood cells and lysing buffer in the gap for a period of time to cause a lysis reaction with the red blood cells; e) introducing a second quantity of lysing buffer into the gap to displace the first quantity of lysing buffer and a first quantity of red blood cell debris out of the gap; f) introducing a first quantity of wash buffer into the gap to quench the lysis reaction and displace the second quantity of lysing buffer and a second quantity of red blood cell debris out of the gap; and g) introducing a second quantity of wash buffer into the gap to flow washed white blood cells out of the housing.

A system consists of a detector and a detection card (9); the detector comprises a separation-detection turntable (4), a drive motor (3), a detection unit (5), a control and analysis unit (1), and a display unit (2); more than one detection card positions are arranged on the separation-detection turntable (4), and the separation-detection turntable (4) is driven by the drive motor (3) to rotate, so as to mix, separate and detect samples in the detection card; and the detection cards (9) are loaded on the detection card positions on the turntable, the detection card (9) is internally divided into more than two inner cavity pools, the divided inner cavity pools are respectively connected by narrow channels with a cross-sectional area less than 60% of a maximum cross-sectional area of each inner cavity pool, and all the inner cavity pools are unidirectionally connected in series.

Separation of the cellular components of whole blood, or other biological fluid, from plasma or serum can be achieved for assay analysis. A device for facilitating separation can include, for example, a capillary tube that accurately draws target blood volume, a pad that chemically interacts with red-blood cells, such that the red blood cells become chemically and/or physically trapped within pad material, a mechanism for plasma recovery from the pad upon diffusion or active mixing, and a dropper tip that facilitates dispensing the mixture onto a test device. The treatment of the cellular components can be performed prior to contact with a buffer solution, so release of the cellular components into the buffer solution is reduced or prevented. Additional filtration can be provided to filter any remaining cellular components in the mixture.

The present invention departs from using traditional modes of cell-to-cell communication and concerns exerting influence on a first cell or cell population by bringing a second cell or cell population into proximity with the first cell population without the use of mediating diffusible factors. Cell to cell communications traditionally occur by way of a variety of mechanisms including, for example, by direct coupling through gap junctions using antigen presentation or using ligand receptor interactions.

Methods for separating blood plasma from whole blood in the absence of performing centrifugation are provided. The method combines mechanical filtration and blood cell aggregation and is adapted for use in POC clinical testing.

A chip includes a micro-channel unit for hydraulically classifying cells in a blood sample. In a micro-channel unit, liquid flowing from a sub channel into a main channel pushes cells flowing in the main channel toward a side thereof on which a removal channel and a collection channel are disposed. Fluid containing non-nucleated RBCs among the pushed cells enters the removal channel, so that the non-nucleated RBCs are removed from a blood sample. A plurality of micro-channel units having the same patterns as each other are repeatedly stacked in a height direction. Inlets of the main channels, inlets of the sub channels, outlets of the removal channels, outlets of the collection channels, and outlets of the main channels, which are provided in the micro-channel units, are connected to respective pillar channels penetrating each of layers in a traversing manner.

Provided is a treatment method for damaging an erythrocyte and a leukocyte while suppressing damage to cells other than blood cells present in blood. In an embodiment, the disclosure relates to a method for treating a sample containing blood components, the method including mixing a sample containing blood components with a surfactant A, where the surfactant A is a nonionic surfactant represented by General formula R.sup.1—O—(EO)n-R.sup.2 (I).

Provided is a composition for mononuclear cell-containing plasma separation capable of suppressing a flow of the composition for mononuclear cell-containing plasma separation during storage, capable of satisfactorily forming a partition during centrifugation, and capable of yielding mononuclear cell-containing plasma with little admixture of blood cell components other than mononuclear cells. The composition for mononuclear cell-containing plasma separation according to the present invention contains an organic component having fluidity at 25° C. and two or more kinds of inorganic fine powders, and has a specific gravity of 1.060 or more and 1.080 or less at 25° C.

Blood containing cells is brought into contact with a porous surface of a porous material before classification of the cells in the blood by flowing the blood through a microchannel. In an example, the porous material is added to the blood containing the cells and mixed together, thereby bringing the blood containing the cells into contact with the porous surface. In an example, the porous material has particles with the porous surface including polysaccharides. The porous material is added to the blood containing the cells while being suspended in a liquid. In an example, the particles have a predetermined particle size distribution. A median particle size d50V in the volume-based cumulative distribution is 25 to 280 μm.