The present disclosure relates to compositions and methods for determining the quality of stored blood and detecting Autologous Blood Transfusions (ABT) and blood doping.

The present disclosure relates to compositions and methods for determining the quality of stored blood and detecting Autologous Blood Transfusions (ABT) and blood doping.

An object of the present invention is to provide a blood analysis method and a blood test kit, which are for performing quantitative analysis of components by precisely obtaining a dilution factor. According to the present invention, provided is a blood analysis method including a step of diluting a collected blood sample with a diluent solution; a step of determining a dilution factor by using a normal value of a normal component which is homeostatically present in blood; and a step of analyzing a concentration of a target component in the blood sample, in which the blood analysis method uses a member selected from the group consisting of a first storing instrument for storing a diluent solution, a separation instrument for separating and recovering blood plasma from the blood sample diluted with the diluent solution, a holding instrument for holding the separation instrument, a second storing instrument for storing the recovered blood plasma, and a sealing instrument for keeping the stored blood plasma within the second storing instrument, in which the diluent solution defines an amount of the normal component which is derived from the diluent solution and/or the members and may be contained in the diluent solution, and in which a volume of the blood sample is 50 μL or less, and a dilution factor of a blood plasma component in the blood sample is 14 times or more.

The present invention relates to a biological sample quality apparatus for determining the quality of a biological sample. The apparatus includes a sample receiver for receiving the biological sample. One or more light sources are provided for supplying light to the sample. An image sensor is provided for capturing an image of the lit sample. The apparatus also includes an image processor for image processing the captured image to determine the quality of the sample. Advantageously, image processing may be used to determine the quality of a sample for use in collection sites and screening laboratories so that acceptability can be determined prior to analyzing the sample. Determination that the sample is of sufficient quality (e.g. sufficient biomaterial) prior to analyzing saves wastage of laboratory time and expense of materials and chemicals. The apparatus may be in the form of desktop or hand-held portable variations.

Methods and compositions are disclosed for preparing a predetermined standardized calibration curve, which can be stored on a label, barcode or tag. A lyophilized product containing assay reagents for use in a bioassay and a known amount of analyte. A predetermined calibration curve associated with the lyophilized product. A predetermined calibration curve makes the preparation of a new calibration curve unnecessary. The methods and compositions are useful in bioassays such as a FRET assay, a real-time reverse transcription polymerase (RT-PCR) assay, a chemiluminescence assay, or any other bioassay that elicits a detectable response based on a change in, or appearance of, color, fluorescence, or reflectance.

Methods and compositions are disclosed for preparing a predetermined standardized calibration curve, which can be stored on a label, barcode or tag. A lyophilized product containing assay reagents for use in a bioassay and a known amount of analyte. A predetermined calibration curve associated with the lyophilized product. A predetermined calibration curve makes the preparation of a new calibration curve unnecessary. The methods and compositions are useful in bioassays such as a FRET assay, a real-time reverse transcription polymerase (RT-PCR) assay, a chemiluminescence assay, or any other bioassay that elicits a detectable response based on a change in, or appearance of, color, fluorescence, or reflectance.

An exemplary embodiment of the present disclosure provides a biosensor that can facilitate detection of the molecules creatinine, creatine and/or sarcosine in a minimal-equipment, portable, and low-cost fashion. Some aspects of the disclosure relate to methods and systems to enable quantitative measurement of certain biomarkers at the point of care without expensive instrumentation.

An exemplary embodiment of the present disclosure provides a biosensor that can facilitate detection of the molecules creatinine, creatine and/or sarcosine in a minimal-equipment, portable, and low-cost fashion. Some aspects of the disclosure relate to methods and systems to enable quantitative measurement of certain biomarkers at the point of care without expensive instrumentation.

Calibration and/or quality control reagents are disclosed for use in serology immunoassays for antibodies to a microorganism. In the reagents, antibodies specific to an antigen of the microorganism are complexed with anti-human Ig antibody to form a complex. Kits and microfluidics devices containing the reagents are also disclosed, along with methods of producing and using the reagents.

Calibration and/or quality control reagents are disclosed for use in serology immunoassays for antibodies to a microorganism. In the reagents, antibodies specific to an antigen of the microorganism are complexed with anti-human Ig antibody to form a complex. Kits and microfluidics devices containing the reagents are also disclosed, along with methods of producing and using the reagents.