A system includes a focus optic configured to converge an electromagnetic radiation (EMR) beam to a focal region located along an optical axis. The system also includes a detector configured to detect a signal radiation emanating from a predetermined location along the optical axis. The system additionally includes a controller configured to adjust a parameter of the EMR beam based in part on the signal radiation detected by the detector. The system also includes a window located a predetermined depth away from the focal region, between the focal region and the focus optic along the optical axis, wherein the window is configured to make contact with a surface of a tissue.

Apparatus and method for facilitating a microscopic imaging of at least one anatomical structure can be provided. For example, with a spectrally-encoded confocal microscopy (SECM) system, it is possible to provide at least one first electro-magnetic radiation to the anatomical structure(s). In addition, a mobile device can be provided which can communicate with the SECM system. The mobile device can have a sensor arrangement, and with such sensor arrangement, it is possible to receive at least one second electro-magnetic radiation that is based on the first radiation(s) from at least one section of the SECM system. The mobile device can further include a computer arrangement, with which it is possible to display at least one portion of the anatomical structure(s) as a microscopic image based on the second radiation(s) received by the sensor arrangement.

A scanner includes a camera, a light source for generating a probe light incorporating a spatial pattern, an optical system for transmitting the probe light towards the object and for transmitting at least a part of the light returned from the object to the camera, a focus element within the optical system for varying a position of a focus plane of the spatial pattern on the object, unit for obtaining at least one image from said array of sensor elements, unit for evaluating a correlation measure at each focus plane position between at least one image pixel and a weight function, a processor for determining the in-focus position(s) of each of a plurality of image pixels for a range of focus plane positions, or each of a plurality of groups of image pixels for a range of focus plane positions, and transforming in-focus data into 3D real world coordinates.

The present invention relates to a topical agent that binds specifically to myelin basic protein and its method of use and determining myelination in the subject by detecting the agent present in the subject. A kit containing the agent or its derivatives for use in detecting myelin basic protein is also provided.

The present invention is directed to a software algorithm that measures the number of corneal nerve fibers in images captured by microscopy including images from patients obtained by in vivo corneal confocal microscopy, a noninvasive technique. The present invention solves a complicated segmentation problem, by exploiting the piece wise linear nature of the nerve fibers—i.e., the nerves are made up of a lot of straight line segments. The image is split into sub-regions, where each sub-region contains nerves mostly running in the same, straight direction. Having the nerves all in straight-lines within a single 2d image region dramatically simplifies the segmentation problem. The image intensities are summed in the direction of the nerves to reduce the 2d representation to a 1d signal having pronounced peaks where the nerves are located.

Apparatus and method can be provided for obtaining at least one anatomical sample. For example, it is possible to provide and insert a housing into a body or provided on a hydrated anatomical structure. Further, with a source, it is possible to emit an electromagnetic radiation which causes at least the anatomical sample(s) to attach to at least one portion of the housing. A compound can be provided on a surface of the housing, and the source provides the radiation to the compound and changes properties thereof to be adhesive. The source can provide the radiation to the housing, and can change properties of a surface thereof to be adhesive. A component can be provided on a surface of the anatomical structure, and the source provides the radiation to the compound and changes properties thereof to be adhesive.

The invention provides a system, apparatus and method for providing remote and rapid access to image data. The invention can be employed during surgery to enable health care personnel, located away from a location where the surgery is being performed, to view and evaluate tissue excised from a patient during the surgery. Such health care personnel can provide feedback to the surgeon to enhance an outcome of the surgery.

The present disclosure relates to plasma generation systems which utilize plasma for semiconductor processing. The plasma generation system disclosed herein employs a hybrid matching network. The plasma generation system includes a RF generator and a matching network. The matching network includes a first-stage to perform low-Q impedance transformations during high-speed variations in impedance. The matching network includes a second-stage to perform impedance matching for high-Q impedance transformations. The matching network further includes a sensor coupled to the first-stage and the second-stage to calculate the signals that are used to engage the first and second-stages. The matching network includes a first-stage network that is agile enough to tune each state in a modulated RF waveform and a second-stage network to tune a single state in a RF modulated waveform. The plasma generation system also includes a plasma chamber coupled to the matching network.

A device and method and system for utilizing confocal measurement and an optical arrangement to produce 3D color images. A color measurement optical path and a 3D measurement optical path may coincide at least at an object side of the device, and a minimal amount of light is extracted from a monitoring beam for color measurement without affecting 3D measurement.

Sub-diffraction limited fluorescent images using a fiber-based stimulated emission depletion (STED) microscope are reported. Both excitation and depletion beams are transported through polarization-maintaining fiber and a lateral resolution of 100 nm has been achieved.