A microdevice includes a plurality of calibration curve liquids, a plurality of first microchannels respectively filled with the plurality of calibration curve liquids, at least one second microchannel filled with a measurement target liquid, and a sealing member that closes openings of the first microchannels to seal the calibration curve liquids. Each of the calibration curve liquids includes a measurement target substance of a predetermined concentration, the predetermined concentration in each of the plurality of calibration curve liquid being mutually different, an antibody that specifically binds to the measurement target substance, and a fluorescent-labeling derivative that fluorescently labels the measurement target substance and competes with the measurement target substance to specifically bind to the antibody. The measurement target liquid includes an unknown concentration of the measurement target substance, the antibody, and the fluorescent-labeling derivative.

A bio-chip is provided. The bio-chip includes a first substrate, a polarizing array, and a plurality of reaction sites. The polarizing array is disposed on the first substrate, and includes first sub-polarizing units having a first polarizing angle and second sub-polarizing units having a second polarizing angle. The first polarizing angle is different from the second polarizing angle. The reaction sites are disposed on the polarizing array. Each of the reaction sites corresponds to one of the first sub-polarizing units or one of the second sub-polarizing units.

An analyzing system includes a detection area with a transparent window past which an analyte moves and emits or transmits light. One or more polarizing elements receive and polarize the light into respective two or more different polarization components. One or more optical detectors receive the respective two or more polarization components and generate respective at least two signals in response. A processor is coupled to the optical detectors and configured to determine a polarization status of the light from the analyte based on the at least two signals.

A bio-detection device is provided. The bio-detection device includes a plurality of pixel units. Each of the pixel units includes a substrate, one or more pairs of reflective sub-polarizing units, and a plurality of reaction sites. The pairs of reflective sub-polarizing units are disposed on the substrate. The difference of the absolute value between respective polarizing angles of the reflective sub-polarizing units in each pair of reflective sub-polarizing units is 90°. The reaction sites are defined above the one or more pairs of reflective sub-polarizing units. The reaction sites and the reflective sub-polarizing units are in one-to-one correspondence.

The invention generally relates to methods for quantifying an amount of enzyme molecules. Systems and methods of the invention are provided for measuring an amount of target by forming a plurality of fluid partitions, a subset of which include the target, performing an enzyme-catalyzed reaction in the subset, and detecting the number of partitions in the subset. The amount of target can be determined based on the detected number.

A fluorescence polarization immunoassay that uses a fluorescent labeling substance in which a single domain antibody is labeled with a fluorescent dye. A fluorescent labeling substance obtained by labeling a single domain antibody, such as a VHH antibody or a vNAR antibody, having a binding ability to a target substance with a fluorescent dye is used. The fluorescence polarization immunoassay includes a binding step for binding the fluorescent labeling substance to a target substance; and a measuring step for measuring a change in the fluorescence polarization of the fluorescent labeling substance to which the target substance is bound.

Methods and systems for insulin detection. The methods may include contacting one or more islets with glucose to produce a first stream that is then contacted with an anti-insulin antibody and a labeled insulin to produce a second stream. The second stream may be analyzed to determine a ratio of antibody-bound (B) labeled insulin to free (F) labeled insulin in the second stream, wherein the ratio of B:F is inversely related to a concentration of target insulin.

The present invention provides a method and an apparatus for easily and precisely detecting an effect of sunscreen (12) based on results of clinical examinations even if various cosmetic layers (14) are provided on the skin (10). The present invention provides an apparatus (1) for detecting a sunscreen (12), comprising: a light source (2); an ultraviolet light pass filter (4) provided between the light source (2) and an irradiation target (10); a visible light sensor (6); and a polarizer (8) provided between the visible light sensor (6) and the irradiation target (10), wherein the ultraviolet light pass filter (4) is configured to pass light (16) having a wavelength in an ultraviolet light range among light emitted from the light source (2), wherein the irradiation target (10) includes at least one substance emitting fluorescent light (20) in response to the light irradiation (16), wherein the polarizer (8) is configured to block at least a part of light (18) emitted from the light source (2) and reflected by the irradiation target (10), and wherein the visible light sensor (6) is configured to determine an intensity of the fluorescent light (20) passing through the polarizer (8).

The invention generally relates to methods for quantifying an amount of enzyme molecules. Systems and methods of the invention are provided for measuring an amount of target by forming a plurality of fluid partitions, a subset of which include the target, performing an enzyme-catalyzed reaction in the subset, and detecting the number of partitions in the sunset. The amount of target can be determined based on the detected number.

Provided are a reagent and a measurement method for enabling a specimen test based on polarization anisotropy to be performed with high sensitivity and within a short period of time. Specifically, provided is an analysis method including measuring a value (R) for polarization anisotropy through use of a luminescent reagent that binds with a target substance, the analysis method including: a reaction step including mixing a sample containing the target substance with the luminescent reagent and a sensitizer, and subjecting the mixture to a reaction to obtain a reaction liquid; and a measurement step of measuring the R of the reaction liquid, the luminescent reagent including a luminescent particle substrate and a hydrophilic layer arranged on an outside of the luminescent particle substrate, the sensitizer containing a hydrophilic polymer.