An atomic level deposition for mass functionalization of a cavity filled with a pathogen sensitive antibody reagent to functionalize each biosensor using atomic level vapor phase deposition enables high volume production of this sensor technology. A biosensor has a first substrate and a second substrate with a cavity formed in the first substrate to form a membrane. Holes are formed through the second substrate. An aluminum oxide layer is formed over the cavity and into the holes to form cores. The cavity is filled with a pathogen sensitive antibody reagent. A biofluid sample with the pathogen is deposited over the membrane. The biofluid is drawn through the cores to mix with the antibody reagent. The antibodies combine with the pathogen to change the impedance along the current path. The presence of the pathogen changes the ionic current flow through the biosensor for a positive detection of the pathogen.

A biological detecting chip and a biological detecting method are disclosed. The biological detecting chip includes a plurality of transistors in parallel. Each of the transistors includes a substrate layer, a floating gate, an extending gate and a biological detecting layer. The substrate layer includes a shared source, a shared drain and a channel area. The floating gate is disposed on the channel area. The floating gate includes a poly oxide layer to extend to an extending metal connect. The extending gate is disposed on the extending metal connect and is electrically connected to the floating gate. The biological detecting layer is disposed on the extending gate. The biological detecting layer includes a plurality of biological probes. The biological detecting layer of the transistors forms a plurality of biological detecting area on the surface of the biological detecting chip.

The present invention provides a bio-sensing device comprising: a source electrode and a drain electrode which are arranged to be spaced apart from each other; a sensing film, which is a channel between the source electrode and the drain electrode and has an electrical resistance value that can be changed by a target analyte; and a gate electrode arranged to be spaced apart from the sensing film, wherein the sensing film is formed from a semiconductor including an indium (In) element.

Methods for detecting analytes using a biological field-effect transistor (BioFET) are disclosed. In some implementations, the method includes exposing a three-dimensional (3D) graphene layer biofunctionalized with a biological recognition element to a target analyte, providing a well region containing an electrolyte solution configured to retain the target analyte, allowing the target analyte to disperse throughout the electrolyte solution and bind with the biological recognition element, detecting a change in electrical properties of the 3D graphene layer in response to the target analyte binding with the biological recognition element, determining a presence of the target analyte based on the change in electrical properties, and outputting an indication of the determined presence of the target analyte. In some aspects, the 3D graphene layer may operate as a channel for the BioFET.

A method for manufacturing a biological field-effect transistor (BioFET) is disclosed. In some implementations, the method may include preparing a carbonaceous dispersion by adding a three-dimensional (3D) graphene into a solvent; depositing the carbonaceous dispersion onto a p-type silicon wafer; spin-coating a positive photoresist over the carbonaceous dispersion; forming source and drain terminals on the p-type silicon wafer, the source and drain terminals in contact with the 3D graphene of the carbonaceous dispersion; removing residual photoresist from the carbonaceous dispersion by placing the p-type silicon wafer in 1-methyl-2-pyrrolidone (NMP); and biofunctionalizing the carbonaceous dispersion with a molecular recognition element configured to alter one or more electrical properties of the Bio-FET in response to exposure of the molecular recognition element to the analyte.

The present disclosure describes a throughput-scalable sensing system. The system includes a plurality of semiconductor dies sharing a common semiconductor substrate and a plurality of transmembrane pore based sensors configured to detect a change of current flow as a result of analyzing biological or chemical samples. Two immediately neighboring transmembrane pore based sensors are arranged on respective two semiconductor dies separated by a dicing street. Each transmembrane pore based sensor is arranged on a separate semiconductor die of the plurality of semiconductor dies. At least one transmembrane pore based sensor includes one or more detection electrodes disposed above the common semiconductor substrate and a lipid bilayer disposed above the one or more detection electrodes.

Disclosed herein are biosensors, and, more particularly, biosensors using carbon nanotubes, an electronic reader for use with the biosensors, and systems and methods employing them. The biosensors employ preserved biologics on the carbon nanotubes which results in shelf-stable, robust biosensors.

An integrated circuit device includes a device layer, an interconnect structure, a conductive layer, a passivation layer and a bioFET. The device layer has a first side and a second side and include source/drain regions and a channel region between the source/drain regions. The interconnect structure is disposed at the first side of the device layer. The conductive layer is disposed at the second side of the device layer. The passivation layer is continuously disposed on the conductive layer and the channel region and exposes a portion of the conductive layer. The bioFET includes the source/drain regions, the channel region and a portion of the passivation layer on the channel region.

An apparatus for separating an analyte from a test sample, such as bacteria from blood components, based on their dielectric properties, localizing or condensing the analyte, flushing substantially all remaining waste products from the test sample, and detecting low concentrations of the analyte. The module array includes a plurality of microfluidic channels with connecting microfluidic waste channels for directing undesired material away from the analyte. An electric field is applied causing a positive dielectrophoretic force to the analyte to capture the analyte. The electric field is applied to at least one electrode having a plurality of concentric rings or concentric arcs extending radially outwards from a center point, electrically connected to a voltage source such that when voltage is applied to the at least one electrode, the concentric rings or concentric arcs alternate in voltage potential.

Biosensor including a device base having a sensor array of light sensors and a guide array of light guides. The light guides have input regions that are configured to receive excitation light and light emissions generated by biological or chemical substances. The light guides extend into the device base toward corresponding light sensors and have a filter material. The device base includes device circuitry electrically coupled to the light sensors and configured to transmit data signals. A passivation layer extends over the device base and forms an array of reaction recesses above the light guides. The biosensor also includes peripheral crosstalk shields that at least partially surround corresponding light guides of the guide array to reduce optical crosstalk between adjacent light sensors.