Dual-gate ion-sensitive field effect transistors (ISFETs) for disease diagnostics are disclosed herein. An exemplary dual-gate ISFET includes a gate structure and a fluidic gate structure disposed over opposite surfaces of a device substrate. The gate structure is disposed over a channel region defined between a source region and a drain region in the device substrate. The fluidic gate structure includes a sensing well that is disposed over the channel region. The sensing well includes a sensing layer and an electrolyte solution. The electrolyte solution includes a constituent that can react with a product of an enzymatic reaction that occurs when an enzyme-modified detection mechanism detects an analyte. The sensing layer can react with a first ion generated from the enzymatic reaction and a second ion generated from a reaction between the product of the enzymatic reaction and the constituent, such that the dual-gate ISFET generates an enhanced electrical signal.

This invention is about a product of a flip chip thin film hybrid screen printed electrode. It combines a primary screen printed electrode (SPE) device and a thin film material coated chip, in order to make a hybridized product. The product is used as a test strip for electrochemical analysis, such as environmental, bio-electrochemical and biomedical sensors. The hybridized electrodes design takes the benefits of low cost of screen printing technology, and high sensitivity of thin film coating nanotechnology. This invention is also about applying a flip chip method to manufacture the hybrid electrode. A chip of thin film material coated solid state substrate is surface mounted to a preliminary perforated SPE by a flip chip method/process. This method/process is fast, easy, cheap, uniform, and suitable for large scale manufacturing.

Frequency division multiplexing-based techniques for FET-based sensor arrays are provided. In one aspect, a sensor device includes: an array of FET-based sensors, wherein the sensors are grouped into multiple channels, and wherein each of the sensors includes an insulator on a substrate, a local gate embedded in the insulator, a channel material over the local embedded gate, and source and drain electrodes in contact with opposite ends of the channel material, and wherein a surface of the channel material is functionalized to react with at least one target molecule. The sensors in a given channel can be modulated (via the local gate) to enable the signal read out from the channel to be divided in the frequency domain based on the different frequencies used to modulate the sensors.

According to one embodiment, a semiconductor device includes FET having a threshold changing according to a chemical state in a gate portion, a time-varying signal application section configured to apply a time-varying signal to at least one of a source, a drain and a back gate of the FET, and a signal reading section configured to read a change in the threshold of the FET resulting from the application

An array of sensor devices, each sensor including a set of semiconducting nanotraces having a width less than about 100 nm is provided. Method for fabricating the arrays is disclosed, providing a top-down approach for large arrays with multiple copies of the detection device in a single processing step. Nanodimensional sensing elements with precise dimensions and spacing to avoid the influence of electrodes are provided. The arrays may be used for multiplex detection of chemical and biomolecular species. The regular arrays may be combined with parallel synthesis of anchor probe libraries to provide a multiplex diagnostic device. Applications for gas phase sensing, chemical sensing and solution phase biomolecular sensing are disclosed.

Provided are a device for detecting chemical and physical phenomenon suitable for high integration, and a method therefor. Rather than using a TG section signal to select pixels that require charge measurement, the on-off timing (the timing for moving the charge from a sensing section to an FD section) of the TG section is harmonized for all pixels, and the release or injection of the charge to the sensing section is separately controlled, whereby the charge is held only in sensing sections of pixels that require charge measurement, and the charge is emptied in sensing sections of pixels that do not require charge measurement. In this state, the TG section of all pixels can be opened at the same time, whereby the charge is transferred to the FD section from only the sensing sections holding a charge, and the charge level of the pixel is detected.

Methods and apparatus relating to FET arrays including large FET arrays for monitoring chemical and/or biological reactions such as nucleic acid sequencing-by-synthesis reactions. Some methods provided herein relate to improving signal (and also signal to noise ratio) from released hydrogen ions during nucleic acid sequencing reactions.

A dyadic sensor includes: an analyte gate; a transition metal dichalcogenide layer disposed on the analyte gate and including a transition metal dichalcogenide; a source electrode disposed on the two-dimensional active layer and in electrical communication with the two-dimensional active layer; a drain electrode disposed on the two-dimensional active layer and in electrical communication with the two-dimensional active layer and in electrical communication with the source electrode via the two-dimensional active layer; and a control gate disposed on the two-dimensional active layer and controlling the communication of electrical current in the two-dimensional active layer between the source electrode and the drain electrode, wherein the electrical current communicated in the two-dimensional active layer is changed in response to a change in an electrical charge present at the analyte gate due to the analyte.

The present invention is intended to provide a method and a device for detecting a biomolecule with high sensitivity and high throughput over a wide dynamic range without requiring concentration adjustments of a sample in advance. The present invention specifically binds charge carriers to a detection target biomolecule, and detects the detection target biomolecule one by one by measuring a current change that occurs as the conjugate of the biomolecule and the charge carriers passes through a micropore. High-throughput detection of a biomolecule sample is possible with an array of detectors.

Sensors having dimensions on the order of nanometers can be arranged in an array. The sensors can detect substances found in an environment. The array of sensors can be disposed on a substrate along with circuitry to control the operation of the array of sensors.