A flying leads assembly includes a flying lead having an inner layer which forms a liquid passage and an outer layer which acts as a sacrificial layer in use, thereby allowing integrity of the passage to be maintained for a long period of time. A further layer is provided intermediate the inner and outer layers which can be used to provide support to the assembly. The flying lead assembly, or a number of assemblies, are then located in a guide with lubricant material.

A centrifuge apparatus includes a plurality of modules, each including a rotatable body, housing and flying leads, assembly. The modules can be selectively interconnected to allow adaptation of the processing capacity of the apparatus to process a liquid to remove components therefrom and/or to process a number of different liquids and allow the separation of one or more types of components therefrom simultaneously.

Disclosed is a device for chromatographic separations comprising: a manifold comprising a manifold body defining an elongate central duct, the central duct comprising a centrally-located closable duct valve providing selective fluid communication between a first portion of the central duct and an opposed second portion of the central duct, a first plurality of connectors, each connector of the first plurality of connectors for connecting to a distinct chromatographic separation column and/or feed or extraction tubing or to a connector of an adjacent manifold; a second plurality of connectors, each connector of the second plurality of connectors for connecting to a distinct chromatographic separation column and/or feed or extraction tubing or to a connector of an adjacent manifold; wherein said manifold body further defines: a first plurality of branch ducts, each branch duct of which extending from the first portion of the central duct to an individual one of the first plurality of connectors, each of the branch ducts of the first plurality of branch ducts comprising a closable branch valve providing selectable fluid communication between a respective connector and the first portion of the central duct, a second plurality of branch ducts, each branch duct of which extending from the second portion of the central duct to an individual one of the second plurality of connectors, each of the branch ducts of the second plurality of branch ducts comprising a closable branch valve providing selectable fluid communication between a respective connector and the second portion of the central duct; first and second ports in fluid communication with the centrally-located closable duct valve wherein said first port communicates with said first portion of the central duct and said second port communicates with said second portion of said central duct, wherein one of said first and second ports is further positioned to communicate with said central duct at a location between the centrally-located closable duct valve and the first and second plurality of branch ducts, respectively.

Systems for measuring a product quality attribute of an analyte of a biological sample include a first flow control device, a sample purification device, a second flow control device in fluid communication with first and second sample analyzers, where the first sample analyzer includes a first chromatography column, and a control unit configured so that during operation of the system, the control unit adjusts a configuration of the second flow control device to direct a portion of the biological sample to one of the first and second sample analyzers, and determines a product quality attribute of an analyte of the biological sample based on an analysis of the portion of the biological sample by the one of the first and second sample analyzers.

Disclosed is a device for chromatographic separations comprising: a manifold comprising a manifold body defining an elongate central duct, the central duct comprising a centrally-located closable duct valve providing selective fluid communication between a first portion of the central duct and an opposed second portion of the central duct, a first plurality of connectors, each connector of the first plurality of connectors for connecting to a distinct chromatographic separation column and/or feed or extraction tubing or to a connector of an adjacent manifold; a second plurality of connectors, each connector of the second plurality of connectors for connecting to a distinct chromatographic separation column and/or feed or extraction tubing or to a connector of an adjacent manifold; wherein said manifold body further defines: a first plurality of branch ducts, each branch duct of which extending from the first portion of the central duct to an individual one of the first plurality of connectors, each of the branch ducts of the first plurality of branch ducts comprising a closable branch valve providing selectable fluid communication between a respective connector and the first portion of the central duct, a second plurality of branch ducts, each branch duct of which extending from the second portion of the central duct to an individual one of the second plurality of connectors, each of the branch ducts of the second plurality of branch ducts comprising a closable branch valve providing selectable fluid communication between a respective connector and the second portion of the central duct; first and second ports in fluid communication with the centrally-located closable duct valve wherein said first port communicates with said first portion of the central duct and said second port communicates with said second portion of said central duct, wherein one of said first and second ports is further positioned to communicate with said central duct at a location between the centrally-located closable duct valve and the first and second plurality of branch ducts, respectively.

Disclosed is a device for chromatographic separations comprising: a manifold comprising a manifold body defining an elongate central duct, the central duct comprising a centrally-located closable duct valve providing selective fluid communication between a first portion of the central duct and an opposed second portion of the central duct, a first plurality of connectors, each connector of the first plurality of connectors for connecting to a distinct chromatographic separation column and/or feed or extraction tubing or to a connector of an adjacent manifold; a second plurality of connectors, each connector of the second plurality of connectors for connecting to a distinct chromatographic separation column and/or feed or extraction tubing or to a connector of an adjacent manifold; wherein said manifold body further defines: a first plurality of branch ducts, each branch duct of which extending from the first portion of the central duct to an individual one of the first plurality of connectors, each of the branch ducts of the first plurality of branch ducts comprising a closable branch valve providing selectable fluid communication between a respective connector and the first portion of the central duct, a second plurality of branch ducts, each branch duct of which extending from the second portion of the central duct to an individual one of the second plurality of connectors, each of the branch ducts of the second plurality of branch ducts comprising a closable branch valve providing selectable fluid communication between a respective connector and the second portion of the central duct; first and second ports in fluid communication with the centrally-located closable duct valve wherein said first port communicates with said first portion of the central duct and said second port communicates with said second portion of said central duct, wherein one of said first and second ports is further positioned to communicate with said central duct at a location between the centrally-located closable duct valve and the first and second plurality of branch ducts, respectively.

A system, module and method for continuous or batch single-pass countercurrent tangential chromatography are disclosed for bind/elute and negative chromatography applications. The system includes binding, washing, elution (for bind/elute), regeneration, and equilibration single-pass modules. The resin slurry flows in a continuous single pass at steady-state through each module, while corresponding buffers flow countercurrent to the slurry facilitating efficient product and impurity extraction. The module and system include retentate pumps for better process robustness and control. A resin tank configured to be reversibly isolated from the single-pass modules facilitates a closed and disposable system. The method includes receiving unpurified product solution and resin slurry, isolating the resin tank, binding product (bind/elute) or impurities (negative) to the resin slurry, washing impurities from the resin slurry, eluting and capturing pure product from the resin slurry (bind/elute), regenerating the resin slurry following elution, and providing buffer solutions to all of the single-pass steps.

A system, module and method for continuous or batch single-pass countercurrent tangential chromatography are disclosed for bind/elute and negative chromatography applications. The system includes binding, washing, elution (for bind/elute), regeneration, and equilibration single-pass modules. The resin slurry flows in a continuous single pass at steady-state through each module, while corresponding buffers flow countercurrent to the slurry facilitating efficient product and impurity extraction. The module and system include retentate pumps for better process robustness and control. A resin tank configured to be reversibly isolated from the single-pass modules facilitates a closed and disposable system. The method includes receiving unpurified product solution and resin slurry, isolating the resin tank, binding product (bind/elute) or impurities (negative) to the resin slurry, washing impurities from the resin slurry, eluting and capturing pure product from the resin slurry (bind/elute), regenerating the resin slurry following elution, and providing buffer solutions to all of the single-pass steps.

There is provided a method and a corresponding system for determining binding capacities of a chromatography column. The method includes detecting a feed signal representative of the composition of a feed material provided to the inlet of the column. The method further includes detecting an effluent signal representative of the composition of the effluent from the column. The method further includes using the feed signal and the effluent signal to determine binding capacities of the column.

There is provided a method and a corresponding system for determining binding capacities of a chromatography column. The method includes detecting a feed signal representative of the composition of a feed material provided to the inlet of the column. The method further includes detecting an effluent signal representative of the composition of the effluent from the column. The method further includes using the feed signal and the effluent signal to determine binding capacities of the column.