A new liquid flow cell assembly for light scattering measurements is disclosed which utilized a floating manifold system. The assembly operates with minimal stacked tolerances by aligning the cell to the windows within a manifold and independently aligning the cell to the read head directly. This configuration enables the ability to replace the flow cell or the flow cell/manifold assembly within a light scattering instrument without the need to realign the flow through elements with the light scattering illumination source while still maintaining reproducible, quality data. Some embodiments employ wide bore cells which enable the measurement of process analytic technology (PAT) including online monitoring of reactions.

Systems and methods are provided for a UV-VIS spectrophotometer, such as a UV-VIS detector unit included in a high-performance liquid chromatography system. In one example, a system for the UV-VIS detector unit may include a first light source, a signal detector, a flow path positioned intermediate the first light source and the signal detector, a second light source, and a reference detector. The first light source, the signal detector, and the flow path may be aligned along a first axis, and the second light source and the reference detector may be aligned along a second axis, different than the first axis.

An apparatus includes a flow cell body, a plurality of electrodes, an imaging assembly, and one or more barrier features. The flow cell body defines one or more flow channels and a plurality of wells defined as recesses in the floor of each flow channel. Each well is fluidically coupled with the corresponding flow channel. The flow cell body further defines interstitial surfaces between adjacent wells. Each well defines a corresponding depth. Each electrode is positioned in a corresponding well of the plurality of wells. The electrodes are to effect writing of polynucleotides in the wells. The imaging assembly is to capture images of polynucleotides written in the wells. The one or more barrier features are positioned in the wells, between the wells, or above the wells. The one or more barrier features contain reactions in each well, reduce diffusion between the wells, or reduce optical cross-talk between the wells.

The present invention relates to method of using spectroscopy for real time measuring of concentration of desired product and using measured data for monitoring and control of chromatography. It develops a method and system for measuring real-time concentration of clarified harvest and that of flow through of loading step of the chromatography and using measured data for determining breakthrough in real-time. The two modes of operation are used viz. first mode (Part A) uses a single near infrared spectroscopy (NIR) flow cell prior to the continuous chromatography column to ensure optimal loading in each cycle based on dynamic binding capacity studies carried out previously with the desired Protein A resin and second mode (Part B) uses two near infrared spectroscopy (NIR) flow cells, one before and one after the column, to detect the breakthrough curve (from 1% breakthrough onwards).

A chromatography detector comprises: a flow cell with a flow path where the sample and a solution flow, the flow cell including outlet tubing that discharges the sample and the solution from the flow path; a connecting member configured to connect the outlet tubing of the flow cell and external tubing outside the flow cell; a wall member including a through-hole; a securing member configured to secure the connecting member to the wall member; and a tray below the securing member. The securing member includes a holder configured to hold the connecting member, an attachment configured to be inserted through the through-hole of the wall member, and a fluid guide formed in such a manner as to, upon the solution leaking out of the connecting member, guide the leaked solution to the tray.

A chromatography detector comprises: a flow cell with a flow path where the sample and a solution flow, the flow cell including outlet tubing that discharges the sample and the solution from the flow path; a connecting member configured to connect the outlet tubing of the flow cell and external tubing outside the flow cell; a wall member including a through-hole; a securing member configured to secure the connecting member to the wall member; and a tray below the securing member. The securing member includes a holder configured to hold the connecting member, an attachment configured to be inserted through the through-hole of the wall member, and a fluid guide formed in such a manner as to, upon the solution leaking out of the connecting member, guide the leaked solution to the tray.

A flow channel structure includes a substrate including a supply flow channel that guides a measurement target liquid toward inside; a separation element accommodating unit that accommodates a separation element that separates components included in the measurement target liquid; and a detection unit that guides the measurement target liquid passing through the separation element accommodating unit, wherein measuring light for measuring information about the components is to be irradiated onto the measurement target liquid. The detection unit includes a measurement flow channel part that guides the measurement target liquid, an incident part that is provided at an end of the measurement flow channel part and that guides the measuring light toward inside the measurement flow channel part, and an emission part that is provided at the other end of the measurement flow channel part and that derives the measuring light from the measurement flow channel part.

The present disclosure relates to optical flow cells for use in chromatography or extraction systems. The optical flow cells include an inlet portion configured to receive a highly compressible fluid and an inlet transition portion or gasket having an internal volume and internal geometry configured to receive the highly compressible fluid from the inlet portion. The optical flow cell also includes an optical path portion configured to receive the highly compressible fluid from the inlet transition portion and direct the highly compressible fluid along an optical flow path. The internal volume and the internal geometry of the inlet transition portion configured to minimize turbulence and eddies within the highly compressible fluid as it travels through the optical flow path.

A method to simulate distillation of a petroleum stream by gas chromatography can include separating the petroleum stream with a gas chromatograph as a function of boiling point; passing the separated petroleum stream through a vacuum ultraviolet detector to yield data comprising a vacuum ultraviolet signal as a function of boiling point; integrating the vacuum ultraviolet signal as a function of boiling point over two or more wavelength ranges to derive relative concentrations of two or more components of the separated petroleum stream that correspond to the two or more wavelength ranges.

A new liquid flow cell assembly for light scattering measurements is disclosed which utilized a floating manifold system. The assembly operates with minimal stacked tolerances by aligning the cell to the windows within a manifold and independently aligning the cell to the read head directly. This configuration enables the ability to replace the flow cell or the flow cell/manifold assembly within a light scattering instrument without the need to realign the flow through elements with the light scattering illumination source while still maintaining reproducible, quality data. Some embodiments employ wide bore cells which enable the measurement of process analytic technology (PAT) including online monitoring of reactions.