The present disclosure provides a technique which makes it possible to evaluate a state of a cell aggregation of one or more spheroids. In the culture state determination device according to the present disclosure, a plurality of light sources sequentially illuminate a plurality of cell aggregations put on an image sensor. The image sensor acquires captured images of the plurality of the cell aggregations each time when the plurality of the light sources illuminate the plurality of the cell aggregations. Control circuitry extracts a region including an image of the cell aggregation in the captured image; generates three-dimensional image information of the region using a plurality of the captured images; extracts an outer shape of the cell aggregation and a cavity part inside the cell aggregation using the three-dimensional image information; calculates a first volume that is a volume based on the outer shape of each of the cell aggregation and a second volume that is a volume of the cavity part based on the cavity part of each of the cell aggregation in the three-dimensional image information; and determines a culture state of the cell aggregations using the first volume and the second volume.

A structure of an electrochemical unit includes a substrate, a first metal layer disposed on the substrate, and an array of electrochemical cells disposed on the first metal layer. The array of the electrochemical cells includes a plurality of electrochemical cells. Each of the electrochemical cells includes the first metal layer disposed on the substrate, a first electrode disposed on the first metal layer, a polymer layer disposed on the substrate and adjacent to the first metal layer and the first electrode. A second metal layer is disposed on the polymer layer, and a second electrode is disposed on the second metal layer. A pore is constituted between the polymer layers of every the two electrochemical cells. A cavity located above the first electrode is defined between every the two electrochemical cells, wherein the cavity is communicated with the pore.

A chemically differentiated sensor array system includes a plurality of environmentally-gated transistors and an environmental gate, wherein the environmental gate includes a liquid solution and each environmentally-gated transistor includes a drain, a source, and a Carbon-based substrate channel, the drain electrically couples to a first location on the substrate channel, the source electrically couples to a second location on the substrate channel separated by a gap from the first location on the substrate channel, the environmental gate covers and contacts the substrate channel, a first insulating layer covers and separates the drain from the environmental gate, and a second insulating layer covers and separates the source from the environmental gate.

According to one embodiment, a biosensor includes a substrate and a sensor matrix that is present in a two-dimensional region on the substrate. The sensor matrix includes a plurality of basic blocks. Each of the basic blocks includes at least three types of sensor elements.

Provided herein are systems and method for measuring cell stiffness. In particular, provided herein are microelectrode configuration and systems for measuring platelet deformation and stiffness.

A system for obtaining a pH measurement includes a disposable probe and a reader. The disposable probe comprises multiple indicating electrodes and at least one reference electrode. The reader is configured to operably engage with the disposable probe and provide pH information of a sample.

Provided herein are systems and method for measuring cell stiffness. In particular, provided herein are microelectrode configuration and systems for measuring platelet deformation and stiffness.

A device for analysis of cells comprises: an integrated circuit arrangement on a substrate; a dielectric layer formed above the integrated circuit arrangement; a microelectrode array layer formed above the dielectric layer, said microelectrode array layer comprising a plurality of individual electrodes, wherein each electrode is connected to the integrated circuit arrangement through a via in the dielectric layer; and wherein a plurality of longitudinal trenches in the dielectric layer and the microelectrode array layer are for stimulating cell growth on a surface of the device

The present disclosure provides a three-dimensional micro-electrode that comprises an electrically conductive, elongate body with: a base that is electrically connectible to a recording system; a tip that is opposite the base and that is configured to establish electrical communication with an excitable cellular-network or a cell therein; and an elongate portion between the base and the tip. The elongate portion is covered with at least one layer of an electrical-insulator coating that extends from the base to proximal the tip. The present disclosure also provides a micro-electrode array comprising at least two three-dimensional micro-electrodes that are electrically connective to at least one recording system. The present disclosure also provides a method of making and using a three-dimensional micro-electrode and an array that comprises three-dimensional micro-electrodes.

A cell observation system 1 is a cell observation system 1 for observing a cell held by a microplate 20 having a plurality of wells 21 arranged therein for holding a sample S including the cell and comprises a microplate holder 11 for mounting the microplate 20, an electrical stimulator 16 arranged with a plurality of electrode pairs 17 including positive and negative electrodes 17b, 17a, and a data analyzer 50 for controlling a position of the electrical stimulator 16 so as to place the electrode pairs 17 within the wells 21 of the microplate 20, while a leading end of the negative electrode 17a on the well 21 side extends longer than a leading end of the positive electrode 17b on the well 21 side.