Identification of autoantibodies associated with Crohn's disease useful in diagnosis and management using an innovative protein array technology, namely nucleic acid programmable protein arrays (NAPPA) and applications relating thereto. Overall, reactivity of IgG autoantibodies was stronger than that of IgA autoantibodies; however, IgA autoantibodies showed greater differential reactivity between cases and controls. Four IgA autoantibodies against SNRPB, PRPH, PTTG1 and SNAI1 were newly identified with sensitivities above 15% at 95% specificity, among which anti-SNRPB-IgA had the highest sensitivity of 24.0%. Autoantibodies associated with specific disease subtypes were also found.

A method for detecting a presence or an absence of at least one zone of inhibition, the method including a step consisting in depositing a volume of the sample in liquid form along a deposition zone extending along an axis at the surface of the agar culture medium and a step consisting in depositing a determined amount of a chemical agent at the surface of the agar culture medium, the deposit defining a potential zone of inhibition, the axis of the zone of deposition of the sample intersecting the potential zone of inhibition.

A self-contained apparatus and methods for detecting the presence of any specified substance in any medium. A sample of the medium is placed in a capsule, along with a solvent and a sensor configured to test for a target analyte. The solvent comes into contact with the medium in the capsule, and the capsule is agitated to create a dispersion in the solvent of a portion of any target analyte present in the medium. A release mechanism configured to cause conduction of the dispersion to the sensor, so that the sensor produces an indication of presence of the target analyte if the target analyte is present in the medium. The apparatus uses a disposable capsule where the medium in question is placed and the disposable capsule is placed inside a reader and analyzed for presence of the harmful substance.

A system and method for detecting harmful substances within a consumable sample comprising: receiving a consumable sample at a first chamber of a test container; transforming the consumable sample into a homogenized sample upon processing of the consumable sample; delivering the homogenized sample to a second chamber of the test container, wherein the second chamber is configured to receive the homogenized sample comprises an outlet port; mixing the homogenized sample with a process reagent within the second chamber, thereby producing a dispersion; transmitting a volume of the dispersion to an analysis chamber, of the test container, configured to position a detection substrate proximal the port of the second chamber and comprising a detection window that enables detection of presence of the allergen; and detecting presence of the allergen within the consumable sample by way of an optical sensor configured to detect signals indicative of the allergen through the detection window.

An automated in situ heat induced antigen recovery and staining method and apparatus for treating a plurality of microscope slides. The process of heat induced antigen recovery and the process of staining the biological specimen on the microscope slide are conducted in the same apparatus, wherein the microscope slides do not need to be physically removed from one apparatus to another. The reaction conditions for treating a slide can preferably be controlled independently, including the individualized application of reagents to each slide and the individualized treatment of each slide.

An electrochemical lateral flow biosensor (ELFB) includes an ELFB strip and electronic detector unit coupled to the strip. The strip includes a polymeric hydrophobic membrane, conjugation pad, sampling pad, screen-printed electrode (SPE), and wick pad. The membrane provides a solid support and enables capillary flow along the strip. The conjugation pad is placed on the membrane at one end of the strip and contains adsorbed dehydrated labelled conjugate particles that are coated with the biorecognition element and coated or filled with the electrochemically active component. The sampling pad is placed on the conjugation pad and provides adsorption of the liquid sample. The SPE is coated with immobilized capture antibodies. The wick pad is placed on the SPE and provides absorption of excess reagents maintaining a lateral flow along the strip. The ELFB can be used for rapid and early detection and quantity analysis of biological liquids and wastewater.

Embodiments disclosed herein relate to a method monitoring a level of a biomarker found in a bodily fluid of a user. The client device receives an indication to capture a biomarker level reading of a test pad on a test strip. The test pad contains a reactant disposed thereon that, when placed into contact with a sample of the bodily fluid, displays a color related to a level of concentration of the biomarker in the bodily fluid. The client device identifies, with a camera, a portion of the test strip containing the test pad displaying the color related to the concentration level of the biomarker. The client device identifies the color displayed on the test strip. The client device correlates the identified color displayed on the test strip to the level of the biomarker. The client device updates the account of the user with the determined biomarker level.

Disclosed is method of improving flow of a liquid sample in an assay device, as well as a method of performing an assay on a liquid sample for the detection of one or more analytes of interest. The methods use the addition of a reagent, such as a wash reagent, to wet the fluid flow path of a sample prior to sample addition, thereby improving flow of the sample through the fluid flow path that has been wetted as opposed to flow of the sample through the fluid flow path that has not been wetted. The reagent wets the fluid flow path between the sample addition zone and the wicking zone.

Provided herein are devices and methods for facile, reliable, quantitative collection and transfer of liquid samples. In particular, the disclosure relates to strip-based systems for the quantitative collection and transfer of biological samples such as proteins, antibodies, DNA, bioanalytical reagents, and chemicals.

A test device for analyzing fluid samples. The test device includes a planar support member for supporting reagent pads, and a handle attached to, or for attaching to the planar support member. The test device can be treated with a fluid sample by disposing a fluid sample on the reagent pads. The fluid sample can be disposed onto the reagent pads by the handle, or by dipping the test device into the fluid sample.