A system and related methods include a durable component, an indicator component including an indicator zone comprising at least one colorimetric analyte sensing element, at least one moisture sensor, and a fluid collection reservoir. The durable component contains at least one spectrophotometer, a computing system, and means for electronic communication between the computing system and at least one external device. The indicator component includes at least one colorimetric analyte sensing element and a fluid transport layer in fluid communication with the indicator zone, and it is arranged and configured for attachment to the durable component. In addition, the moisture sensor is arranged and configured to communicate the presence of moisture to initiate a predetermined delay in measuring the concentration of at least one analyte. The fluid collection reservoir is releasable from at least one of the indicator components and the durable component at a predetermined breaking point for clinical analysis.

A rapid testing mechanism for respiratory viral pathogens includes a filter material positioned to capture exhaled breath particles from a respiratory tract. At least a portion of the filter material includes a pathogen binding adsorptive reagent, wherein the pathogen binding adsorptive reagent is a sulfated cellulose membrane. When the exhaled breath particles pass through the filter material, the following occur: when the binding adsorptive reagent reacts, a positive test for respiratory viral pathogens is indicated by the filter material; and when the pathogen binding adsorptive reagent does not react, a negative test for respiratory viral pathogens is individuated by the filter material.

Provided are a diagnostic system and methods for detecting the presence or absence of one or more targets that represent virus infection, inflammatory diseases and/or respiratory disorders by optically and noninvasively observing changing in color of the diagnostic system upon binding of the antigen of interest. Exemplary diagnostic systems and methods include detection of SARS-CoV-2 virus S protein or receptor of advanced glycation end products (RAGE) for diagnosis of COVID-19 infection or inflammatory/respiratory diseases.

A method for conducting environmental sampling includes determining to environmentally sample a particular paper transit ticket for a specific residue. Using an automated ticket device, at least one chemical reagent is applied to the particular paper transit ticket. The chemical reagent is selected based on the specific residue. The particular paper transit ticket is collected after the particular paper transit ticket was used as a fare media within a transit system. The at least one chemical reagent is analyzed to determine whether any of the specific residue is present.

Multiplex polymeric dye devices are provided. Aspects of the devices include a solid support, and first and second dried polymeric dye compositions distinctly positioned relative to a surface of the solid support. Aspects of the invention further include methods of making and using the devices, e.g., in analyte detection applications, as well as kits containing the devices.

Disclosed are systems and methods for the robust passive detection of airborne toxins using a colorimetric sensor coating onto a optically transparent substrate. In certain embodiments, the substrate is affixed to an adhesive material (tape). In certain embodiments, the sensor and substrate are transparent. In various embodiments, multiple sensors are coated onto selected substrate for the simultaneous detection of multiple toxins. In various embodiments, the sensed or detected toxins include a number of chemical warfare agents and toxic industrial chemicals. In various implementations, the tape is affixed to a remote surface, which may be visually monitored by a camera directly by focusing the camera on the tape or may be affixed to a camera lens by an adhesive backing, such that colorimetric sensor changes may be observed through the lens itself. Sensor claddings consist of optical grade polymers immobilized with colorimetric and/or fluorescent indicators that undergo optical changes upon exposure to their target analyte. Typical substrate cross-linked polymers are urethane acrylate polymer based, co-polymerized with silicone backbone such as dimethyl siloxane, which in general is chemically inert, yet leaves the polymer with the large free-volume necessary for rapid target diffusion. The polymer is cured after immobilization with target indicator mixture, and simultaneously cross-linked by UV light or heat.

Disclosed herein is a functionalized eyewear device that is adapted for collecting and analyzing disease biomarkers. Specifically, exemplified is a contact lens that has aptamer molecules associated therewith for binding to a specific biomarker (or biomarkers). The eyewear device is useful for detecting and diagnosing medical conditions.

A probe compound, contact lens containing bound probe, and method are disclosed for use in detecting analytes in basal tears on the surface of the eye of a subject. The probe composition preferably includes a hydrophilic portion, an analyte-sensitive portion and a fluorophore portion as well as a hydrophobic portion that allows binding to the contact lens material and optionally can modify the binding affinity of the fluorophore probe. The analyte-sensitive portion is configured to bind to a specific analyte in aqueous solution or to be quenched by the analyte. The fluorophore portion is configured to change an optical property of fluorescent light emitted in response to incident excitation light when the probe composition changes between a first state in which the analyte is not bound to the analyte-binding portion and a second state in which the analyte binds to the analyte-binding portion.

A condom for detection of STDs, including an interior condom surface having a control band and a test band, and an exterior condom surface that also has a control band and test band. Each test band allows binding and subsequent detection of STD antigens. A separate developing antibody produces a color change if bound to the antigen/antibody conjugate. The developing antibody may be present on the condom or applied separately after use.

A collection and test device for a rapid test is provided. The device comprises a test fluid accommodation part having a test fluid accommodation space, a test paper accommodation part having a test paper accommodation space, and a collection probe having a channel for the test fluid to flow out from the collection probe. The two ends of the test paper accommodation part are respectively connected to the test fluid accommodation part and the collection probe, and the test paper accommodation space communicates with the channel of the collection probe. The test fluid accommodation space and the test paper accommodation space are separated from each other by a temporary barrier. The temporary barrier can be manually removed or broken to make the test fluid accommodation space communicate with the test paper accommodation space. The device of the present invention can provide the test results conveniently and rapidly.