Disclosed herein is a functionalized eyewear device that is adapted for collecting and analyzing disease biomarkers. Specifically, exemplified is a contact lens that has aptamer molecules associated therewith for binding to a specific biomarker (or biomarkers). The eyewear device is useful for detecting and diagnosing medical conditions.

A method of determining the presence of a threshold concentration of leukocyte cells in a sample, the method comprising (i) contacting the sample with a leukocyte detection means comprising (a1) an indicator compound; and (ii) examining the leukocyte detection means to determine whether leukocytes are present.

A reagent test paddle includes a contamination detection medium, a reference color bar, at least one chemical test medium, and a unique identifier. The contamination detection medium includes a reagent that changes color in the presence or when exposed to a hostile or inhospitable environment. Each chemical test medium includes a regent that is responsive to a respective analyte in a biological sample. The reference color bar includes reference color samples of different colors. The unique identifier, like a serial number, identifies the particular paddle and its chemical test medium so it can be uniquely and anonymously associated with a user. A method includes capturing and interpreting digital images of a biologically unexposed and subsequently exposed reagent test paddle at various delay times within an automatically calibrated environment; locating the paddle in a plurality of digital images, extracting the reference color bar and locating the contamination detection medium and chemical test medium in each digital image. Color changes of the chemical test medium and contamination medium are detected at various delay times after sample exposure. To determine validity of test results, the method further compares the detected colors of the contamination detection medium with predetermined colors expected for no contamination and contamination.

The present invention relates to a method for identifying deviations from a standard composition of urine and from standard physicochemical parameters, which method comprises separating a urine collection element from a diagnostic layer, wetting a urine indicator strip via the urine collection element, and reading the result of the analysis by comparison against a colorimetric scale. The invention likewise relates to an analysis kit for identifying any deviations from a standard.

Technology is disclosed for a cat litter that can be used to diagnose feline medical conditions. A cat litter contains an additive, e.g., a chemical agent, such as a natural and/or synthetic agent, that changes in color when it comes in contact with cat urine or feces. Based on the color it can be determined whether the cat has a medical condition, such as particular illness. For example, if the litter turns yellow when it comes in contact with the urine, it can indicate that the cat is healthy. If the litter turns to a color other than yellow, e.g., green, it can indicate that the cat has some illness. The cat then be taken to a veterinary doctor for further diagnosis. The chemical agent aids in detection and diagnosis through interaction of materials that change colors to indicate reactions with specific chemicals, cells, molecules, DNA and/or materials. The technology can be implemented for medical diagnosis and detectionfor humans and/or animals.

Multiplex polymeric dye devices are provided. Aspects of the devices include a solid support, and first and second dried polymeric dye compositions distinctly positioned relative to a surface of the solid support. Aspects of the invention further include methods of making and using the devices, e.g., in analyte detection applications, as well as kits containing the devices.

Systems, devices, kits, and methods for detecting and quantifying targeted compounds within a liquid (such as urine) are provided. Such systems, devices, and methods may be autonomous, noninvasive, and provide quick and accurate results. The systems and devices are at least partially disposable (single-use) and configured to be embedded within or applied to a conventional diaper or the like. Methods for using the systems and devices hereof include receiving a liquid to be tested within a portion of a disposable device, allowing the liquid to traverse through one or more channels defined within the device in a controlled fashion, reacting the liquid with one or more chemical reagents, using a sensing unit to collect photocurrent data regarding the chemical reach on(s), and wirelessly transmitting that data to a computing unit for storage and quantitative analysis.

Multiplex polymeric dye devices are provided. Aspects of the devices include a solid support, and first and second dried polymeric dye compositions distinctly positioned relative to a surface of the solid support. Aspects of the invention further include methods of making and using the devices, e.g., in analyte detection applications, as well as kits containing the devices.

A lateral flow device is disclosed. The device comprises a test layer comprising a test region between opposite first and second end regions of the test layer, a first permeable layer having a first contact area in contact with the first end region, and a second permeable layer having a second contact area in contact with the first end region. The device further comprises a first non-permeable layer between the first and second permeable layers and having a third contact area with the first end region. The first contact area and the second contact area are separated by the third contact area. The device further comprises a sample pad in fluid contact with the first and second permeable layers for delivery of a sample fluid to the first and second contact regions. The second contact area is further from the second end region than the first contact area is from the second end region.

Urine analysis system that couples or integrates with a toilet. Wirelessly links to a computer, such as a user's mobile device to accept control inputs and report urine test results. Accepts user input to initiate testing and deploys a urine collector into the toilet bowl above the water to collect a urine sample. The collected urine sample is dispensed onto multiple regions of a test matrix that may perform many urine tests simultaneously. A single test matrix may include multiple types of tests, such as immunochromatography and colorimetric assays. An optical imaging system detects reaction of the urine with reagents integrated into the test matrix. Analysis of images may be performed locally or on a remote server. The system may store an inventory of test matrices to support daily testing for long durations, for example 6 months or more before refilling.