Integrated devices that include a sample preparation component integrated with a detection component are disclosed. The sample preparation component may be a digital microfluidics module or a surface acoustic wave module which modules are used for combing a sample droplet with a reagent droplet and for performing additional sample preparation step leading to a droplet that contains beads/particles/labels that indicate presence or absence of an analyte of interest in the sample. The beads/particles/labels may be detected by moving the droplet to the detection component of the device, which detection component includes an array of wells.

A test device by immunochromatography comprises a case including a dropping window for a specimen and a detection window, and a test piece in the case. The test piece includes first and second detection portions, and a dropping area common to the first and second detection portions. When the specimen is dropped on the dropping area through the dropping window, the specimen migrates downstream and the same specimen reaches the first and second detection portions. The first detection portion traps a complex of a first labeled body and a first antibody contained in the specimen. The second detection portion traps a complex of a second labeled body and a second antibody contained in the specimen. The first antibody is produced by vaccination or infection with a predetermined virus. The second antibody is produced by infection with the virus. Thus, a test result from each of the first and second detection portions, both visually observed through the detection window, can be determined in parallel and at the same time.

This invention is in the field of medical devices. Specifically, the present invention provides fluidic systems having a plurality of reaction sites surrounded by optical barriers to reduce the amount of optical cross-talk between signals detected from various reaction sites. The invention also provides a method of manufacturing fluidic systems and methods of using the systems.

A method is provided for manipulating objects in a cavity including a liquid, the method including providing in at least one region of the cavity objects capable of absorbing light in a given wavelength range, forming an aggregate of the objects by submitting them to an acoustic field, and disrupting the aggregate by submitting the aggregate to a light beam emitting at the given wavelength range. Also provided is a device for manipulating objects.

The present disclosure relates to biosensors (10) having a receptor layer (5) and a mediator layer (6), the receptor layer including ethylene receptor molecules. The present disclosure also relates to sensor units (20) comprising one or more biosensors (10) and a controller (11). In some embodiments, one or more sensor units (20) may be in wireless communication with a receiver module or a network gateway.

The invention generally relates to mass tag analysis for rare cells and cell free molecules. In certain embodiments, the invention provides an apparatus including an essentially non-absorbent membrane having at least one pore, a microwell operably associated with the essentially non-absorbent membrane, and an electric field generator. The apparatus may be configured such that an electric field produced by the electric field generator operably interacts with a sample in the microwell and expels a droplet of the sample through the at least one pore in the essentially non-absorbent membrane. In certain embodiments, apparatuses of the invention are used for detection, and optionally quantification, of a target analyte from a heterogeneous sample, such as a rare target analyte (e.g., rare cell) from a biological sample.

The invention provides methods and tools for the directed and indirect detection of infection with microorganisms pathogens in biological and non- biological samples, and specifically applications of XRF (X-ray fluorescence) methodology for the detection of infections with viral and bacterial pathogens responsible for the widespread epidemics in mammals and humans, including the current pandemic of COVID-19.

A system for time-resolved fluoroimmunoassay detection is disclosed. The system comprises a receptacle with a sample volume adapted for receiving a sample therein; a light source adapted to emit pulsed excitation light illumination optics adapted to collect the pulsed excitation light from the light source and to deliver the pulsed excitation light to the sample volume in the receptacle; a detection device adapted for gated detection of fluorescence radiation at least in a detection spectral range; detection optics adapted to collect fluorescence radiation from the receptacle at least in the detection spectral range and deliver the fluorescence radiation to the detection device; and an optical filter device configured for the separation of excitation light and detection light. A method for time-resolved fluoroimmunoassay detection is also provided.

A method for determining the concentration of lactulose in a blood sample comprises the steps of converting lactulose in the blood sample into an entity including galactose, and converting the galactose into D-galacto-hexodialdose and hydrogen peroxide, which is detected. The detected quantity is processed to generate an output which is representative of the concentration of lactulose in the blood sample.

The present invention discloses a pencil-like optical fiber sensor probe, including an inner tube, a light screening casing, a clamping device, an optical fiber and an optical probe; a portable immunosensor, including the pencil-like optical fiber sensor probe, an immersion immune response reagent strip, a touch-screen computer, a compact battery-powered sensitive photon counting detector and a case; and a use of the immunosens in detecting inflammatory markers. The design of the pencil-like optical sensor probe greatly simplifies the immune analysis process by combining the immersion immune response reagent strip. Each optical probe allows for up to 10 immunoassays, which reduces the experimental cost and avoids frequent replacement of the probe. The integrated detecting system is powered by battery which is suitable for in-situ analysis and detection. The sensor also has a high stability and sensitivity.