A chromogenic assay includes a substrate comprising an array of 5 or more dyes which react with volatile organic compounds, wherein the dyes are chromogenic when reacted with volatile organic chemical biomarkers, wherein the volatile organic chemical biomarkers comprise acids, alcohols, aldehydes, alkenes, amines, antioxidants, aromatic compounds, esters, ethylene, lactones, ketones, organosulfur compounds, sulfides, reactive oxygen species, terpenes, or a combination thereof. A method of detecting volatile organic chemical biomarkers includes contacting the chromogenic assay with a sample or sample headspace, wherein the sample or sample headspace is suspected of containing volatile organic chemical biomarkers, and identifying, based on a colorimetric pattern on the chromogenic assay after contacting, the source of the volatile organic chemical biomarkers. Also included are articles and systems including the chromogenic assay.

To provide a particle labeling reagent having low luminescent characteristics and improved water solubility.

Provided is a particle labeling reagent containing a compound represented by the following general formula (I-1) or (I-2).

##STR00001## (In the above general formula (I-1), p represents an integer of 1 to 3. In the above general formula (I-1), M represents a hydrogen atom or a mono- to tri-valent metal atom. In the above general formula (I-1), L.sup.1 represents a single bond or a (p+1)-valent group. In the above general formulas (I-1) and (I-2), L.sup.2 and L.sup.3 each independently represent a hydrogen atom or a photodegradable protecting group, and L.sup.2 and L.sup.3 may be the same or different. Provided that at least one of L.sup.2 and L.sup.3 represents a photodegradable protecting group. In the above general formula (I-2), L.sup.4 represents a monovalent group.)

The inventive concept discloses a time series quantification method of supravital dye uptake of a cell using a lens-free imaging system.

Activity-based probes that can be used to selectively identify and characterize enzymes that are involved in different phases of xenobiotic metabolism in a host and its microbiota population(s) are described. The activity-based probes described specifically label only their target active enzymes involved in xenobiotic metabolism and therefore provide a measurement of true protein functional activity rather than transcript or protein abundance. The activity-based probes also provide multimodal profiling of these active enzymes. Methods for preparing the activity based probes and exemplary methods for their use also are disclosed.

Aspects of the application provide methods and compositions for identifying and evaluating putative therapeutic agents for cancer by live cell imaging. Cell samples comprising cancerous cells that have been pre-treated with a test agent are contacted with a BH3 peptide, and samples are imaged by live cell imaging over a time interval. Methods of the application can be used to determine whether a patient is likely to benefit from treatment with a particular test agent.

The disclosed technology relates to chemical entities for the detection of wounds, e.g., chronic wounds or infected wounds, including compositions, substrates, kits, dressing materials, and articles, and systems containing such compounds. The disclosed technology further relates to methods of using these compositions, kits and systems in diagnostic assays, and in the diagnosis and/or detection of chronic or infected wounds based on enzymatic action on specific moieties and/or reaction sites. The disclosed technology additionally relates to detection of pathogenic, e.g., bacterial and/or viral substances, such as enzymes and substrates, at the wound situs. Additional disclosure relates to methods of characterizing wounds based on expression of a plurality of markers and using such information to treat, manage, and follow-up patients suffering from chronic or infected wounds.

The present invention generally relates to method for forming an immunolabeling complex, the immunolabeling complex comprising a labeled monovalent biotin-binding composition. The invention also related to the use of the antibody-reporter molecule complex for detecting a target in a sample.

Disclosed herein are immunoassays for detecting an anti-thyroid peroxidase antibody in a biological sample from a subject and/or diagnosing a thyroid disease in a subject. The disclosed immunoassays employ a recombinant cynomolgus monkey thyroid peroxidase (rTPO) and assess the level of anti-thyroid peroxidase antibody-induced formation or disruption of complexes comprising a solid support and the rTPO.

Rapid detection of analytes including, for example, systems, kits, and methods for growth, isolation, and/or monitoring of analytes are generally disclosed. In some embodiments, the systems and methods described herein are generally directed to the capture and/or concentrating of a target species (e.g., analyte) to be detected and/or monitored. In some embodiments, the materials, systems, and methods described herein may be used to create luminescent signals in response to the presence of selected analytes such as bacteria, viruses, and parasites. In some cases, the target analyte is a pathogenic bacteria, a pathogenic virus, a pathogenic parasite, or toxin.

Dried dye reagent devices are provided. Aspects of the devices include a container having positioned therein one or more dried dye compositions that include one or more dyes stably associated with a high surface area solid support. Aspects of the invention further include methods of making and using the devices, e.g., in analyte detection applications, as well as kits containing the devices.